SSGCID: Target Page

BaheA.01664.a

Two-component sensor histidine kinase (BatR-BatS two-component system)

CENTER ID:	BaheA.01664.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01664.a.B2.GE35048	kinase domain		333	594
BaheA.01664.a.B3.GE37257	periplasmic domain		68	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01664.a.B2.PS01512	kinase domain		333	594

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.72
RefSeq:	YP_032926.1
UniProt:	A0A0H3LVQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKNTQLETL QKITPNGLPS SPFSILSDLR FRTQRLLRQL LFSSLTRRIV ILNLAALAIL VTGILYLNQF RDGLIEAKIK SLCIQGKIIA GAIAASATVD TNSILIDPQK LLELQAGESV TPAPQSTDSW DFPINPEQVA PLLRRLITPK TTRARIYDRD ATLLLDSRVL YSSGEVFSYD LPPLKTEKKL WERLYSWFSN ALYGNGLAID REQAKKRGIV HPEVYRALNG SLATAKRRNR QGQLIVSVAV PVQRYRAVVG ALLLSTTGSD IDDIVKGERL VIFKVFAVVG SVLLVLSLFL AHTIAHPLSK LSASANRVRN GNNKRVEIPD FSMREDEIGH LSTSIRDMTN ALYMRIEAIE RFAADVSHEL KNPLTSLRSA VETLPLAKNE EAQEQLIEII QHDVRRLDRL ITDISDASRL DAELARETAQ IVDVTLLLES LVHAVQEVYR NRQNIDISLN IIPRPHGKPY LVLGHELRLG QVISNLIENA RSFIPHDNGK ICITMKSNAA NLVLIIEDNG PGIRSENIER IFERFYTDRP SEDTFGQNSG LGLSISRQII EAHNGTITAE NIVDPELENS KTGARFIIVL PFAR

NT Sequence

atgacaaaaa atactcaact ggaaaccctg caaaaaataa caccaaatgg tcttccctca tctccctttt ctatactttc cgatttgcgc tttcggacac aacgtcttct cagacaattg cttttttcca gtcttacacg ccgtattgtc atcttaaatc ttgctgccct tgctattctg gttacaggca ttttatatct taatcaattt cgtgacggtt taattgaagc gaaaatcaaa agcttatgta tccaaggaaa aattatcgct ggagccattg ctgcttctgc aacagtagat acaaattcta ttctcattga tccccaaaag cttttagaat tacaagctgg agaaagcgtt acacctgcac ctcaatcaac agactcttgg gactttccca ttaatcctga acaagttgct cctcttttac gacgcctcat cacacctaaa acgacaaggg cacgtatcta tgatcgtgat gctactttgc ttcttgattc acgcgttctt tattctagtg gtgaagtttt cagctatgat ttacccccac ttaaaactga aaaaaaactg tgggagcgcc tctattcatg gttttcaaat gcactttatg gcaatggtct tgccatcgat agggagcaag caaaaaaacg gggtattgtt catccagaag tttaccgagc tcttaatgga tcacttgcaa ccgctaaacg acgcaacaga caaggtcaat taatcgtttc tgtcgccgtg cctgttcaac gctatcgagc tgtggtaggt gcgcttcttc tttcaaccac cggttcggat attgatgaca ttgtaaaagg tgaaagactg gttattttta aagtttttgc cgtcgtaggc agcgtgcttt tggtactttc tctcttttta gcccatacga ttgcgcatcc attgagtaaa ttatctgcct ccgccaatcg tgtgcgcaat ggcaataata agcgtgttga aattcccgat ttttcaatgc gcgaagatga aattggtcat ctttcaacct ctattcgtga catgaccaat gccctttata tgcgtattga agccattgag cgctttgctg ctgatgtaag ccacgaatta aaaaatccgc ttacctcact gcgtagtgct gttgaaaccc ttcctttggc taaaaatgaa gaagcacagg aacaattgat tgaaattatt caacatgatg tgcgtcgtct tgaccgtttg attactgata tttctgatgc atcgcgactt gatgcagaac ttgctcgtga aacggcgcaa attgttgatg taacactact tttggaaagc cttgttcatg ctgttcagga agtgtaccgt aataggcaaa acattgatat aagccttaat attatcccac gtcctcatgg aaaaccttac cttgtattgg gacatgaact ccgtttaggg caagtaattt ccaatctcat tgaaaatgca cgttctttca ttccccatga taacggcaaa atttgtataa ctatgaaaag taatgctgca aatcttgtct taattattga agataatggc cctggtattc gctcagaaaa tattgaacgc atttttgaac gcttttatac tgatcgacca agcgaagata catttggaca aaactcagga ctcggtcttt ctattagtcg acaaatcatt gaggctcata atgggacaat tacagcagaa aatattgttg accccgaact tgaaaatagt aaaactggcg cacgttttat tattgtcctt cccttcgctc gataa

Details for BaheA.01664.a.B2.GE35048

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR EDEIGHLSTS IRDMTNALYM RIEAIERFAA DVSHELKNPL TSLRSAVETL PLAKNEEAQE QLIEIIQHDV RRLDRLITDI SDASRLDAEL ARETAQIVDV TLLLESLVHA VQEVYRNRQN IDISLNIIPR PHGKPYLVLG HELRLGQVIS NLIENARSFI PHDNGKICIT MKSNAANLVL IIEDNGPGIR SENIERIFER FYTDRPSEDT FGQNSGLGLS ISRQIIEAHN GTITAENIVD PELENSKTGA RFIIVLPFAR

Validated NT Sequence

cctannttac tcacttatcg agcgaaggga aggacaataa taaaacgtgc gccagtttta ctattttcaa gttcggggtc aacaatattt tctgctgtaa ttgtcccatt atgagcctca atgatttgtc gactaataga aagaccgagt cctgagtttt gtccaaatgt atcttcgctt ggtcgatcag tataaaagcg ttcaaaaatg cgttcaatat tttctgagcg aataccaggg ccattatctt caataattaa gacaagattt gcagcattac ttttcatagt tatacaaatt ttgccgttat catggggaat gaaagaacgt gcattttcaa tgagattgga aattacttgc cctaaacgga gttcatgtcc caatacaagg taaggttttc catgaggacg tgggataata ttaaggctta tatcaatgtt ttgcctatta cggtacactt cctgaacagc atgaacaagg ctttccaaaa gtagtgttac atcaacaatt tgcgccgttt cacgagcaag ttctgcatca agtcgcgatg catcagaaat atcagtaatc aaacggtcaa gacgacgcac atcatgttga ataatttcaa tcaattgttc ctgtgcttct tcatttttag ccaaaggaag ggtttcaaca gcactacgca gtgaggtaag cggatttttt aattcgtggc ttacatcagc agcaaagcgc tcaatggctt caatacgcat ataaagggca ttggtcatgt cacgaataga ggttgaaaga tgaccaattt catcttcgcg catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg aagatgaaat tggtcatctt tcaacctcta ttcgtgacat gaccaatgcc ctttatatgc gtattgaagc cattgagcgc tttgctgctg atgtaagcca cgaattaaaa aatccgctta cctcactgcg tagtgctgtt gaaacccttc ctttggctaa aaatgaagaa gcacaggaac aattgattga aattattcaa catgatgtgc gtcgtcttga ccgtttgatt actgatattt ctgatgcatc gcgacttgat gcagaacttg ctcgtgaaac ggcgcaaatt gttgatgtaa cactactttt ggaaagcctt gttcatgctg ttcaggaagt gtaccgtaat aggcaaaaca ttgatataag ccttaatatt atcccacgtc ctcatggaaa accttacctt gtattgggac atgaactccg tttagggcaa gtaatttcca atctcattga aaatgcacgt tctttcattc cccatgataa cggcaaaatt tgtataacta tgaaaagtaa tgctgcaaat cttgtcttaa ttattgaaga taatggccct ggtattcgct cagaaaatat tgaacgcatt tttgaacgct tttatactga tcgaccaagc gaagatacat ttggacaaaa ctcaggactc ggtctttcta ttagtcgaca aatcattgag gctcataatg ggacaattac agcagaaaat attgttgacc ccgaacttga aaatagtaaa actggcgcac gttttattat tgtccttccc ttcgctcgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaheA.01664.a.B3.GE37257

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QFRDGLIEAK IKSLCIQGKI IAGAIAASAT VDTNSILIDP QKLLELQAGE SVTPAPQSTD SWDFPINPEQ VAPLLRRLIT PKTTRARIYD RDATLLLDSR VLYSSGEVFS YDLPPLKTEK KLWERLYSWF SNALYGNGLA IDREQAKKRG IVHPEVYRAL NGSLATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTTG SDIDDIVKGE R

Validated NT Sequence

agccggatcc tntcttactc acttatcttt caccttttac aatgtcatca atatccgaac cggtggttga aagaagaagc gcacctacca cagctcgata gcgttgaaca ggcacggcga cagaaacgat taattgacct tgtctgttgc gtcgtttagc ggttgcaagt gatccattaa gagctcggta aacttctgga tgaacaatac cccgtttttt tgcttgctcc ctatcgatgg caagaccatt gccataaagt gcatttgaaa accatgaata gaggcgctcc cacagttttt tttcagtttt aagtgggggt aaatcatagc tgaaaacttc accactagaa taaagaacgc gtgaatcaag aagcaaagta gcatcacgat catagatacg tgcccttgtc gttttaggtg tgatgaggcg tcgtaaaaga ggagcaactt gttcaggatt aatgggaaag tcccaagagt ctgttgattg aggtgcaggt gtaacgcttt ctccagcttg taattctaaa agcttttggg gatcaatgag aatagaattt gtatctactg ttgcagaagc agcaatggct ccagcgataa tttttccttg gatacataag cttttgattt tcgcttcaat taaaccgtca cgaaattgat tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHNQ FRDGLIEAKI KSLCIQGKII AGAIAASATV DTNSILIDPQ KLLELQAGES VTPAPQSTDS WDFPINPEQV APLLRRLITP KTTRARIYDR DATLLLDSRV LYSSGEVFSY DLPPLKTEKK LWERLYSWFS NALYGNGLAI DREQAKKRGI VHPEVYRALN GSLATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTTGS DIDDIVKGER

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcaat ttcgtgacgg tttaattgaa gcgaaaatca aaagcttatg tatccaagga aaaattatcg ctggagccat tgctgcttct gcaacagtag atacaaattc tattctcatt gatccccaaa agcttttaga attacaagct ggagaaagcg ttacacctgc acctcaatca acagactctt gggactttcc cattaatcct gaacaagttg ctcctctttt acgacgcctc atcacaccta aaacgacaag ggcacgtatc tatgatcgtg atgctacttt gcttcttgat tcacgcgttc tttattctag tggtgaagtt ttcagctatg atttaccccc acttaaaact gaaaaaaaac tgtgggagcg cctctattca tggttttcaa atgcacttta tggcaatggt cttgccatcg atagggagca agcaaaaaaa cggggtattg ttcatccaga agtttaccga gctcttaatg gatcacttgc aaccgctaaa cgacgcaaca gacaaggtca attaatcgtt tctgtcgccg tgcctgttca acgctatcga gctgtggtag gtgcgcttct tctttcaacc accggttcgg atattgatga cattgtaaaa ggtgaaagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaheA.01664.a.B2.PS01512

PURIFICATION DATe:	5/31/2012
CONCENTRATION:	87.85mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCG AAGATGAAAT TGGTCATCTT TCAACCTCTA TTCGTGACAT GACCAATGCC CTTTATATGC GTATTGAAGC CATTGAGCGC TTTGCTGCTG ATGTAAGCCA CGAATTAAAA AATCCGCTTA CCTCACTGCG TAGTGCTGTT GAAACCCTTC CTTTGGCTAA AAATGAAGAA GCACAGGAAC AATTGATTGA AATTATTCAA CATGATGTGC GTCGTCTTGA CCGTTTGATT ACTGATATTT CTGATGCATC GCGACTTGAT GCAGAACTTG CTCGTGAAAC GGCGCAAATT GTTGATGTAA CACTACTTTT GGAAAGCCTT GTTCATGCTG TTCAGGAAGT GTACCGTAAT AGGCAAAACA TTGATATAAG CCTTAATATT ATCCCACGTC CTCATGGAAA ACCTTACCTT GTATTGGGAC ATGAACTCCG TTTAGGGCAA GTAATTTCCA ATCTCATTGA AAATGCACGT TCTTTCATTC CCCATGATAA CGGCAAAATT TGTATAACTA TGAAAAGTAA TGCTGCAAAT CTTGTCTTAA TTATTGAAGA TAATGGCCCT GGTATTCGCT CAGAAAATAT TGAACGCATT TTTGAACGCT TTTATACTGA TCGACCAAGC GAAGATACAT TTGGACAAAA CTCAGGACTC GGTCTTTCTA TTAGTCGACA AATCATTGAG GCTCATAATG GGACAATTAC AGCAGAAAAT ATTGTTGACC CCGAACTTGA AAATAGTAAA ACTGGCGCAC GTTTTATTAT TGTCCTTCCC TTCGCTCGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT