SSGCID: Target Page

BaheA.01643.a

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase

CENTER ID:	BaheA.01643.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01643.a.B1.GE43958	Full length( BaheA.01643.a )		1	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1179
RefSeq:	YP_033901.1
UniProt:	A0A0H3LZ19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFGTIFTEC LEDENLSSME ITGISADSRQ ILPGYIFVAL QGHQDDGKHY INDALKRGAQ AIVTDCNVVL ENLSVPLLRV LDARHSLAVA AARFYGSQPE TVVAVTGTSG KTSVVSFLRQ IWTKVGLCAA SIGTLGVVSP KRNDYGSLTT PDPVFLQRLL SEIANEGVTH AALEASSHGL DQCRLDGVRL TAAAFTNLGR DHMDYHTSVE DYLRAKMRLF DTLLPRDASA LIFADDIYSQ KVIDTLTQAR RHVLTIGRKG QFITINRIEH QRSKQCVECR LENNIYTFNL PLAGDFQVNN ALMAAGLAIS TGISPDKVFR SLETLQGAPG RLELVGKTEN NASVYIDYAH KPEALEQVLL SVRPFTQGRL ILVFGCGGDR DQGKRPLMGK IAENKADIVI ITDDNPRTEM PAKIRKDILQ AAPKAIEIAD RGEAITYAVG LLKAGDTLII AGKGHENGQI IGQKTYPFSD RLKAIEALQE RIR

NT Sequence

ATGTTGTTTG GAACAATTTT TACAGAATGT CTTGAAGATG AGAATCTTTC TTCAATGGAA ATAACAGGAA TCAGTGCAGA TTCTCGGCAA ATCTTGCCAG GCTATATTTT TGTAGCTCTT CAAGGTCATC AAGACGATGG GAAACACTAT ATCAATGATG CACTAAAACG TGGTGCACAA GCAATCGTTA CAGATTGTAA TGTTGTTTTG GAGAATTTAT CTGTTCCCCT TTTGCGTGTT TTGGATGCTC GTCATAGCTT AGCGGTAGCA GCTGCTCGTT TTTATGGTTC TCAACCTGAA ACTGTCGTTG CTGTGACTGG GACAAGTGGA AAAACATCAG TTGTCTCTTT TCTTCGGCAA ATTTGGACAA AGGTTGGATT ATGTGCTGCG AGTATAGGAA CGCTAGGTGT TGTTTCTCCT AAACGAAATG ATTATGGTTC TCTTACAACA CCTGACCCGG TCTTCTTGCA GCGTCTTCTT TCTGAAATTG CTAATGAAGG TGTTACACAT GCAGCTCTTG AAGCATCTTC GCATGGGCTT GATCAGTGCC GACTTGACGG AGTACGTCTT ACTGCTGCTG CCTTTACCAA TTTAGGACGT GATCATATGG ATTATCACAC CAGCGTAGAG GATTATTTGC GTGCTAAAAT GAGGTTATTT GATACACTTT TACCTCGAGA TGCTTCTGCT TTGATTTTTG CGGATGATAT TTATTCGCAA AAGGTTATTG ATACCCTCAC ACAAGCACGT CGTCATGTTT TAACAATTGG GCGTAAAGGG CAATTTATCA CTATTAATCG TATTGAACAT CAACGTTCGA AACAGTGTGT TGAATGTCGT TTGGAAAACA ATATCTACAC ATTTAATTTA CCTTTAGCGG GAGATTTTCA AGTGAATAAT GCACTTATGG CGGCGGGACT TGCGATTTCA ACAGGTATAT CACCTGATAA GGTATTTCGT TCTCTTGAAA CTTTGCAGGG AGCACCTGGT CGTTTAGAGT TGGTTGGAAA GACAGAAAAT AATGCTTCTG TTTATATTGA TTATGCACAT AAACCGGAAG CTTTGGAACA AGTGCTGCTT TCTGTTCGTC CCTTCACGCA AGGGCGTTTA ATCCTTGTTT TTGGTTGTGG AGGTGATCGC GATCAAGGAA AAAGACCTTT AATGGGAAAA ATTGCAGAAA ATAAAGCTGA TATTGTTATT ATAACGGATG ATAACCCTCG CACGGAAATG CCAGCGAAAA TACGCAAGGA TATTTTACAA GCAGCGCCAA AAGCCATCGA AATAGCAGAT CGTGGTGAAG CTATTACTTA TGCAGTTGGG CTTTTAAAAG CAGGGGATAC ATTAATCATT GCTGGAAAAG GTCATGAGAA TGGTCAAATT ATAGGGCAAA AAACATATCC TTTTTCAGAT CGTTTGAAAG CGATTGAAGC TTTACAGGAA CGGATTAGA

Details for BaheA.01643.a.B1.GE43958

HARVESTED ON:	12/3/2020
SEQUENCED ON:	12/16/2020
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHML FGTIFTECLE DENLSSMEIT GISADSRQIL PGYIFVALQG HQDDGKHYIN DALKRGAQAI VTDCNVVLEN LSVPLLRVLD ARHSLAVAAA RFYGSQPETV VAVTGTSGKT SVVSFLRQIW TKVGLCAASI GTLGVVSPKR NDYGSLTTPD PVFLQRLLSE IANEGVTHAA LEASSHGLDQ CRLDGVRLTA AAFTNLGRDH MDYHTSVEDY LRAKMRLFDT LLPRDASALI FADDIYSQKV IDTLTQARRH VLTIGRKGQF ITINRIEHQR SKQCVECRLE NNIYTFNLPL AGDFQVNNAL MAAGLAISTG ISPDKVFRSL ETLQGAPGRL ELVGKTENNA SVYIDYAHKP EALEQVLLSV RPFTQGRLIL VFGCGGDRDQ GKRPLMGKIA ENKADIVIIT DDNPRTEMPA KIRKDILQAA PKAIEIADRG EAITYAVGLL KAGDTLIIXG KGXEXXXXIX XXXXPXXXXX

Validated NT Sequence

atggctcacc accaccacca ccatatgttg tttggaacaa tttttacaga atgtcttgaa gatgagaatc tttcttcaat ggaaataaca ggaatcagtg cagattctcg gcaaatcttg ccaggctata tttttgtagc tcttcaaggt catcaagacg atgggaaaca ctatatcaat gatgcactaa aacgtggtgc acaagcaatc gttacagatt gtaatgttgt tttggagaat ttatctgttc cccttttgcg tgttttggat gctcgtcata gcttagcggt agcagctgct cgtttttatg gttctcaacc tgaaactgtc gttgctgtga ctgggacaag tggaaaaaca tcagttgtct cttttcttcg gcaaatttgg acaaaggttg gattatgtgc tgcgagtata ggaacgctag gtgttgtttc tcctaaacga aatgattatg gttctcttac aacacctgac ccggtcttct tgcagcgtct tctttctgaa attgctaatg aaggtgttac acatgcagct cttgaagcat cttcgcatgg gcttgatcag tgccgacttg acggagtacg tcttactgct gctgccttta ccaatttagg acgtgatcat atggattatc acaccagcgt agaggattat ttgcgtgcta aaatgaggtt atttgataca cttttacctc gagatgcttc tgctttgatt tttgcggatg atatttattc gcaaaaggtt attgataccc tcacacaagc acgtcgtcat gttttaacaa ttgggcgtaa agggcaattt atcactatta atcgtattga acatcaacgt tcgaaacagt gtgttgaatg tcgtttggaa aacaatatct acacatttaa tttaccttta gcgggagatt ttcaagtgaa taatgcactt atggcggcgg gacttgcgat ttcaacaggt atatcacctg ataaggtatt tcgttctctt gaaactttgc agggagcacc tggtcgttta gagttggttg gaaagacaga aaataatgct tctgtttata ttgattatgc acataaaccg gaagctttgg aacaagtgct gctttctgtt cgtcccttca cgcaagggcg tttaatcctt gtttttggtt gtggaggtga tcgcgatcaa ggaaaaagac ctttaatggg aaaaattgca gaaaataaag ctgatattgt tattataacg gatgataacc ctcgcacgga aatgccagcg aaaatacgca aggatatttt acaagcagcg ccaaaagcca tcgaaatagc agatcgtggt gaagctatta cttatgcagt tgggctttta aaagcagggg atacattaat cattgntgga aaaggtcang agaangnnnn nnnnatagnn cannnnncnn ntccnnnnnn ncntngtnng aaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgt ttggaacaat ttttacagaa tgtcttgaag atgagaatct ttcttcaatg gaaataacag gaatcagtgc agattctcgg caaatcttgc caggctatat ttttgtagct cttcaaggtc atcaagacga tgggaaacac tatatcaatg atgcactaaa acgtggtgca caagcaatcg ttacagattg taatgttgtt ttggagaatt tatctgttcc ccttttgcgt gttttggatg ctcgtcatag cttagcggta gcagctgctc gtttttatgg ttctcaacct gaaactgtcg ttgctgtgac tgggacaagt ggaaaaacat cagttgtctc ttttcttcgg caaatttgga caaaggttgg attatgtgct gcgagtatag gaacgctagg tgttgtttct cctaaacgaa atgattatgg ttctcttaca acacctgacc cggtcttctt gcagcgtctt ctttctgaaa ttgctaatga aggtgttaca catgcagctc ttgaagcatc ttcgcatggg cttgatcagt gccgacttga cggagtacgt cttactgctg ctgcctttac caatttagga cgtgatcata tggattatca caccagcgta gaggattatt tgcgtgctaa aatgaggtta tttgatacac ttttacctcg agatgcttct gctttgattt ttgcggatga tatttattcg caaaaggtta ttgataccct cacacaagca cgtcgtcatg ttttaacaat tgggcgtaaa gggcaattta tcactattaa tcgtattgaa catcaacgtt cgaaacagtg tgttgaatgt cgtttggaaa acaatatcta cacatttaat ttacctttag cgggagattt tcaagtgaat aatgcactta tggcggcggg acttgcgatt tcaacaggta tatcacctga taaggtattt cgttctcttg aaactttgca gggagcacct ggtcgtttag agttggttgg aaagacagaa aataatgctt ctgtttatat tgattatgca cataaaccgg aagctttgga acaagtgctg ctttctgttc gtcccttcac gcaagggcgt ttaatccttg tttttggttg tggaggtgat cgcgatcaag gaaaaagacc tttaatggga aaaattgcag aaaataaagc tgatattgtt attataacgg atgataaccc tcgcacggaa atgccagcga aaatacgcaa ggatatttta caagcagcgc caaaagccat cgaaatagca gatcgtggtg aagctattac ttatgcagtt gggcttttaa aagcagggga tacattaatc attgctggaa aaggtcatga gaatggtcaa attatagggc aaaaaacata tcctttttca gatcgtttga aagcgattga agctttacag gaacggatta gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat