BaheA.01599.a

serine hydroxymethyltransferase

CENTER ID: BaheA.01599.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01599.a.A1.GE27631 Full length( BaheA.01599.a ) 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01599.a.A1.PS00403 Full length( BaheA.01599.a ) 1 437

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.807
RefSeq: YP_033566.1
UniProt: Q6G3L3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKQGNDTQK RFFNDNLQTV DVAIFDAIRG EFERQQHEIE LIASENIVSR AVLEAQGSVL TNKYAEGYPG KRYYGGCQFV DVIENLAIER AKKLFGADFA NVQANSGSQM NQAVFLALLK PGDTFMGLDL NSGGHLTHGS SVNMSGKWFK SISYGVRKED QLLDMEAVER LAKEHKPKLI IAGGSAYSRL WDWKKFREIA DEIGAYLLVD MSHIAGLVAG GVHPSPVPHA HIVTTTTHKS LRGPRGGLIL TNDEILAKKI NSAIFPGLQG GPLMHVIAAK AVAFEEALQP VFKDYSANVV ANAKTLAKTL QSNGFDIVSG GTDNHLLLVD LRSKKVTGKC AELALGRAHI TCNKNSIPFD LETPFITSGI RLGSPAATTR GFAENEFIEI AHMISEILDN LGMAKSDEDN SAVEMVVRKK VEDMTNKFPL YSYLHTC
NT Sequence
ATGACCAAGC AAGGAAATGA TACACAAAAG CGTTTTTTTA ATGATAATTT ACAAACAGTT GATGTTGCAA TCTTTGATGC GATCAGGGGA GAGTTTGAGC GTCAACAACA TGAAATTGAG TTGATTGCGT CAGAGAATAT TGTTTCAAGG GCAGTCCTTG AAGCTCAAGG ATCAGTTTTG ACCAATAAAT ATGCAGAAGG TTATCCAGGA AAGCGCTATT ATGGAGGTTG TCAATTTGTT GATGTGATTG AAAATTTAGC AATTGAACGA GCTAAAAAGC TTTTTGGTGC TGATTTTGCA AACGTTCAGG CTAATTCTGG TAGCCAAATG AATCAGGCTG TGTTTTTAGC TTTACTAAAG CCGGGTGATA CATTTATGGG GTTGGATTTA AATTCCGGGG GTCATCTTAC ACATGGTTCA TCCGTTAATA TGTCAGGAAA ATGGTTTAAA TCTATTTCCT ATGGTGTACG TAAAGAAGAT CAGCTTCTTG ATATGGAGGC TGTTGAACGC CTTGCAAAGG AACATAAACC CAAACTTATT ATAGCAGGTG GATCGGCTTA TTCTCGCTTA TGGGATTGGA AAAAATTTCG TGAAATTGCA GATGAGATTG GTGCTTATCT CCTCGTTGAT ATGTCTCATA TTGCTGGTTT AGTTGCTGGT GGTGTTCATC CTTCACCTGT TCCACATGCG CACATTGTGA CAACGACAAC GCATAAATCA CTGAGAGGTC CTCGTGGTGG TTTAATTTTA ACAAATGATG AAATTTTAGC CAAAAAAATT AACTCAGCGA TTTTCCCTGG TCTTCAGGGT GGTCCTTTAA TGCACGTGAT TGCAGCTAAA GCTGTTGCAT TTGAGGAAGC TTTGCAACCT GTTTTCAAAG ATTATAGTGC CAATGTTGTT GCTAATGCAA AAACCTTAGC AAAAACACTA CAGAGCAACG GTTTTGATAT TGTTTCTGGC GGTACGGACA ATCATTTATT GTTGGTTGAT TTGCGTTCAA AAAAAGTGAC AGGAAAATGT GCCGAATTGG CTTTAGGGCG TGCTCATATT ACCTGCAATA AAAATAGTAT TCCTTTTGAT CTTGAAACAC CATTTATAAC ATCTGGAATT CGTCTTGGTT CACCTGCTGC GACGACACGT GGTTTTGCAG AAAATGAATT TATTGAAATT GCTCATATGA TTTCGGAGAT TCTTGATAAT CTCGGTATGG CAAAAAGTGA TGAAGACAAT AGTGCCGTTG AAATGGTTGT TAGGAAAAAG GTGGAAGATA TGACAAATAA ATTTCCTCTT TACTCTTACC TTCACACTTG T
Details for BaheA.01599.a.A1.GE27631
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 50kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKQGNDTQ KRFFNDNLQT VDVAIFDAIR GEFERQQHEI ELIASENIVS RAVLEAQGSV LTNKYAEGYP GKRYYGGCQF VDVIENLAIE RAKKLFGADF ANVQANSGSQ MNQAVFLALL KPGDTFMGLD LNSGGHLTHG SSVNMSGKWF KSISYGVRKE DQLLDMEAVE RLAKEHKPKL IIAGGSAYSR LWDWKKFREI ADEIGAYLLV DMSHIAGLVA GGVHPSPVPH AHIVTTTTHK SLRGPRGGLI LTNDEILAKK INSAIFPGLQ GGPLMHVIAA KAVAFEEALQ PVFKDYSANV VANAKTLAKT LQSNGFDIVS GGTDNHLLLV DLRSKKVTGK CAELALGRAH ITCNKNSIPF DLETPFITSG IRLGSPAATT RGFAENEFIE IAHMISEILD NLGMAKSDED NSAVEMVVRK KVEDMTNKFP LYSYLHTC
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaccaag caaggaaatg atacacaaaa gcgttttttt aatgataatt tacaaacagt tgatgttgca atctttgatg cgatcagggg agagtttgag cgtcaacaac atgaaattga gttgattgcg tcagagaata ttgtttcaag ggcagtcctt gaagctcaag gatcagtttt gaccaataaa tatgcagaag gttatccagg aaagcgctat tatggaggtt gtcaatttgt tgatgtgatt gaaaatttag caattgaacg agctaaaaag ctttttggtg ctgattttgc aaacgttcag gctaattctg gtagccaaat gaatcaggct gtgtttttag ctttactaaa gccgggtgat acatttatgg ggttggattt aaattccggg ggtcatctta cacatggttc atccgttaat atgtcaggaa aatggtttaa atctatttcc tatggtgtac gtaaagaaga tcagcttctt gatatggagg ctgttgaacg ccttgcaaag gaacataaac ccaaacttat tatagcaggt ggatcggctt attctcgctt atgggattgg aaaaaatttc gtgaaattgc agatgagatt ggtgcttatc tcctcgttga tatgtctcat attgctggtt tagttgctgg tggtgttcat ccttcacctg ttccacatgc gcacattgtg acaacgacaa cgcataaatc actgagaggt cctcgtggtg gtttaatttt aacaaatgat gaaattttag ccaaaaaaat taactcagcg attttccctg gtcttcaggg tggtccttta atgcacgtga ttgcagctaa agctgttgca tttgaggaag ctttgcaacc tgttttcaaa gattatagtg ccaatgttgt tgctaatgca aaaaccttag caaaaacact acagagcaac ggttttgata ttgtttctgg cggtacggac aatcatttat tgttggttga tttgcgttca aaaaaagtga caggaaaatg tgccgaattg gctttagggc gtgctcatat tacctgcaat aaaaatagta ttccttttga tcttgaaaca ccatttataa catctggaat tcgtcttggt tcacctgctg cgacgacacg tggttttgca gaaaatgaat ttattgaaat tgctcatatg atttcggaga ttcttgataa tctcggtatg gcaaaaagtg atgaagacaa tagtgccgtt gaaatggttg ttaggaaaaa ggtggaagat atgacaaata aatttcctct ttactcttac cttcacactt gttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKQGNDTQ KRFFNDNLQT VDVAIFDAIR GEFERQQHEI ELIASENIVS RAVLEAQGSV LTNKYAEGYP GKRYYGGCQF VDVIENLAIE RAKKLFGADF ANVQANSGSQ MNQAVFLALL KPGDTFMGLD LNSGGHLTHG SSVNMSGKWF KSISYGVRKE DQLLDMEAVE RLAKEHKPKL IIAGGSAYSR LWDWKKFREI ADEIGAYLLV DMSHIAGLVA GGVHPSPVPH AHIVTTTTHK SLRGPRGGLI LTNDEILAKK INSAIFPGLQ GGPLMHVIAA KAVAFEEALQ PVFKDYSANV VANAKTLAKT LQSNGFDIVS GGTDNHLLLV DLRSKKVTGK CAELALGRAH ITCNKNSIPF DLETPFITSG IRLGSPAATT RGFAENEFIE IAHMISEILD NLGMAKSDED NSAVEMVVRK KVEDMTNKFP LYSYLHTC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaagcaa ggaaatgata cacaaaagcg tttttttaat gataatttac aaacagttga tgttgcaatc tttgatgcga tcaggggaga gtttgagcgt caacaacatg aaattgagtt gattgcgtca gagaatattg tttcaagggc agtccttgaa gctcaaggat cagttttgac caataaatat gcagaaggtt atccaggaaa gcgctattat ggaggttgtc aatttgttga tgtgattgaa aatttagcaa ttgaacgagc taaaaagctt tttggtgctg attttgcaaa cgttcaggct aattctggta gccaaatgaa tcaggctgtg tttttagctt tactaaagcc gggtgataca tttatggggt tggatttaaa ttccgggggt catcttacac atggttcatc cgttaatatg tcaggaaaat ggtttaaatc tatttcctat ggtgtacgta aagaagatca gcttcttgat atggaggctg ttgaacgcct tgcaaaggaa cataaaccca aacttattat agcaggtgga tcggcttatt ctcgcttatg ggattggaaa aaatttcgtg aaattgcaga tgagattggt gcttatctcc tcgttgatat gtctcatatt gctggtttag ttgctggtgg tgttcatcct tcacctgttc cacatgcgca cattgtgaca acgacaacgc ataaatcact gagaggtcct cgtggtggtt taattttaac aaatgatgaa attttagcca aaaaaattaa ctcagcgatt ttccctggtc ttcagggtgg tcctttaatg cacgtgattg cagctaaagc tgttgcattt gaggaagctt tgcaacctgt tttcaaagat tatagtgcca atgttgttgc taatgcaaaa accttagcaa aaacactaca gagcaacggt tttgatattg tttctggcgg tacggacaat catttattgt tggttgattt gcgttcaaaa aaagtgacag gaaaatgtgc cgaattggct ttagggcgtg ctcatattac ctgcaataaa aatagtattc cttttgatct tgaaacacca tttataacat ctggaattcg tcttggttca cctgctgcga cgacacgtgg ttttgcagaa aatgaattta ttgaaattgc tcatatgatt tcggagattc ttgataatct cggtatggca aaaagtgatg aagacaatag tgccgttgaa atggttgtta ggaaaaaggt ggaagatatg acaaataaat ttcctcttta ctcttacctt cacacttgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BaheA.01599.a.A1.PS00403
PURIFICATION DATe: 11/10/2009
CONCENTRATION: 27.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKQGNDTQ KRFFNDNLQT VDVAIFDAIR GEFERQQHEI ELIASENIVS RAVLEAQGSV LTNKYAEGYP GKRYYGGCQF VDVIENLAIE RAKKLFGADF ANVQANSGSQ MNQAVFLALL KPGDTFMGLD LNSGGHLTHG SSVNMSGKWF KSISYGVRKE DQLLDMEAVE RLAKEHKPKL IIAGGSAYSR LWDWKKFREI ADEIGAYLLV DMSHIAGLVA GGVHPSPVPH AHIVTTTTHK SLRGPRGGLI LTNDEILAKK INSAIFPGLQ GGPLMHVIAA KAVAFEEALQ PVFKDYSANV VANAKTLAKT LQSNGFDIVS GGTDNHLLLV DLRSKKVTGK CAELALGRAH ITCNKNSIPF DLETPFITSG IRLGSPAATT RGFAENEFIE IAHMISEILD NLGMAKSDED NSAVEMVVRK KVEDMTNKFP LYSYLHTC
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaccaag caaggaaatg atacacaaaa gcgttttttt aatgataatt tacaaacagt tgatgttgca atctttgatg cgatcagggg agagtttgag cgtcaacaac atgaaattga gttgattgcg tcagagaata ttgtttcaag ggcagtcctt gaagctcaag gatcagtttt gaccaataaa tatgcagaag gttatccagg aaagcgctat tatggaggtt gtcaatttgt tgatgtgatt gaaaatttag caattgaacg agctaaaaag ctttttggtg ctgattttgc aaacgttcag gctaattctg gtagccaaat gaatcaggct gtgtttttag ctttactaaa gccgggtgat acatttatgg ggttggattt aaattccggg ggtcatctta cacatggttc atccgttaat atgtcaggaa aatggtttaa atctatttcc tatggtgtac gtaaagaaga tcagcttctt gatatggagg ctgttgaacg ccttgcaaag gaacataaac ccaaacttat tatagcaggt ggatcggctt attctcgctt atgggattgg aaaaaatttc gtgaaattgc agatgagatt ggtgcttatc tcctcgttga tatgtctcat attgctggtt tagttgctgg tggtgttcat ccttcacctg ttccacatgc gcacattgtg acaacgacaa cgcataaatc actgagaggt cctcgtggtg gtttaatttt aacaaatgat gaaattttag ccaaaaaaat taactcagcg attttccctg gtcttcaggg tggtccttta atgcacgtga ttgcagctaa agctgttgca tttgaggaag ctttgcaacc tgttttcaaa gattatagtg ccaatgttgt tgctaatgca aaaaccttag caaaaacact acagagcaac ggttttgata ttgtttctgg cggtacggac aatcatttat tgttggttga tttgcgttca aaaaaagtga caggaaaatg tgccgaattg gctttagggc gtgctcatat tacctgcaat aaaaatagta ttccttttga tcttgaaaca ccatttataa catctggaat tcgtcttggt tcacctgctg cgacgacacg tggttttgca gaaaatgaat ttattgaaat tgctcatatg atttcggaga ttcttgataa tctcggtatg gcaaaaagtg atgaagacaa tagtgccgtt gaaatggttg ttaggaaaaa ggtggaagat atgacaaata aatttcctct ttactcttac cttcacactt gttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKQGNDTQ KRFFNDNLQT VDVAIFDAIR GEFERQQHEI ELIASENIVS RAVLEAQGSV LTNKYAEGYP GKRYYGGCQF VDVIENLAIE RAKKLFGADF ANVQANSGSQ MNQAVFLALL KPGDTFMGLD LNSGGHLTHG SSVNMSGKWF KSISYGVRKE DQLLDMEAVE RLAKEHKPKL IIAGGSAYSR LWDWKKFREI ADEIGAYLLV DMSHIAGLVA GGVHPSPVPH AHIVTTTTHK SLRGPRGGLI LTNDEILAKK INSAIFPGLQ GGPLMHVIAA KAVAFEEALQ PVFKDYSANV VANAKTLAKT LQSNGFDIVS GGTDNHLLLV DLRSKKVTGK CAELALGRAH ITCNKNSIPF DLETPFITSG IRLGSPAATT RGFAENEFIE IAHMISEILD NLGMAKSDED NSAVEMVVRK KVEDMTNKFP LYSYLHTC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAAGCAA GGAAATGATA CACAAAAGCG TTTTTTTAAT GATAATTTAC AAACAGTTGA TGTTGCAATC TTTGATGCGA TCAGGGGAGA GTTTGAGCGT CAACAACATG AAATTGAGTT GATTGCGTCA GAGAATATTG TTTCAAGGGC AGTCCTTGAA GCTCAAGGAT CAGTTTTGAC CAATAAATAT GCAGAAGGTT ATCCAGGAAA GCGCTATTAT GGAGGTTGTC AATTTGTTGA TGTGATTGAA AATTTAGCAA TTGAACGAGC TAAAAAGCTT TTTGGTGCTG ATTTTGCAAA CGTTCAGGCT AATTCTGGTA GCCAAATGAA TCAGGCTGTG TTTTTAGCTT TACTAAAGCC GGGTGATACA TTTATGGGGT TGGATTTAAA TTCCGGGGGT CATCTTACAC ATGGTTCATC CGTTAATATG TCAGGAAAAT GGTTTAAATC TATTTCCTAT GGTGTACGTA AAGAAGATCA GCTTCTTGAT ATGGAGGCTG TTGAACGCCT TGCAAAGGAA CATAAACCCA AACTTATTAT AGCAGGTGGA TCGGCTTATT CTCGCTTATG GGATTGGAAA AAATTTCGTG AAATTGCAGA TGAGATTGGT GCTTATCTCC TCGTTGATAT GTCTCATATT GCTGGTTTAG TTGCTGGTGG TGTTCATCCT TCACCTGTTC CACATGCGCA CATTGTGACA ACGACAACGC ATAAATCACT GAGAGGTCCT CGTGGTGGTT TAATTTTAAC AAATGATGAA ATTTTAGCCA AAAAAATTAA CTCAGCGATT TTCCCTGGTC TTCAGGGTGG TCCTTTAATG CACGTGATTG CAGCTAAAGC TGTTGCATTT GAGGAAGCTT TGCAACCTGT TTTCAAAGAT TATAGTGCCA ATGTTGTTGC TAATGCAAAA ACCTTAGCAA AAACACTACA GAGCAACGGT TTTGATATTG TTTCTGGCGG TACGGACAAT CATTTATTGT TGGTTGATTT GCGTTCAAAA AAAGTGACAG GAAAATGTGC CGAATTGGCT TTAGGGCGTG CTCATATTAC CTGCAATAAA AATAGTATTC CTTTTGATCT TGAAACACCA TTTATAACAT CTGGAATTCG TCTTGGTTCA CCTGCTGCGA CGACACGTGG TTTTGCAGAA AATGAATTTA TTGAAATTGC TCATATGATT TCGGAGATTC TTGATAATCT CGGTATGGCA AAAAGTGATG AAGACAATAG TGCCGTTGAA ATGGTTGTTA GGAAAAAGGT GGAAGATATG ACAAATAAAT TTCCTCTTTA CTCTTACCTT CACACTTGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A