SSGCID: Target Page

BaheA.01386.a

Phosphomethylpyrimidine kinase

CENTER ID:	BaheA.01386.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01386.a.A1.GE26712	Full length( BaheA.01386.a )		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1895
RefSeq:	YP_034320.1
UniProt:	A0A0H3M4U8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLQVSQDTL FIPRILSIAG TDPSGGAGMQ ADLKVFSAMK TYGMSVVTAV VAQNTQGVRA FQALDASFVA DQIDAVFEDI HVDAVKIGMV ANAQIARTVA ERLAYHKARF IVFDPVMVAK SGDVLLKSDA IEIMRDVLVP MSTLITPNLP EASLLLGCEV KWSLDAMYQY GPQLLTLGCH AVLLKGGHLD VFSRTNEANY DYSSPDLYCD CEDLVMLEAP RFITTHDHGT GCTISAAIAA LLPTKPLVDA VKQAKDYLNG ALSTSSALQV GKGRGPLHHF YELWKNV

NT Sequence

ATGAAATTAC AGGTTAGCCA AGACACTCTT TTTATTCCAC GGATTTTATC TATTGCGGGA ACAGATCCTT CTGGTGGCGC TGGAATGCAA GCGGATTTGA AAGTTTTTTC AGCTATGAAA ACCTATGGAA TGAGTGTGGT GACCGCTGTG GTTGCACAAA ATACTCAAGG TGTACGTGCT TTTCAGGCGT TGGATGCTTC TTTTGTTGCT GACCAAATTG ATGCGGTTTT TGAAGATATT CATGTTGATG CTGTTAAAAT TGGTATGGTA GCAAATGCTC AAATTGCTCG GACTGTCGCA GAACGTCTTG CTTATCACAA AGCGCGCTTC ATTGTTTTTG ATCCCGTTAT GGTGGCAAAA AGTGGCGATG TCCTTTTAAA GTCTGATGCG ATTGAAATTA TGCGTGATGT TCTTGTGCCG ATGTCAACTT TGATCACGCC AAATTTACCT GAGGCATCTC TATTGTTGGG ATGTGAGGTA AAGTGGTCTT TAGACGCTAT GTACCAATAT GGTCCACAGC TTCTGACTCT AGGGTGTCAT GCAGTTCTTT TGAAGGGTGG GCATTTGGAC GTTTTTTCTC GTACAAATGA GGCGAATTAT GATTATTCTA GTCCAGATCT TTATTGTGAT TGTGAAGATC TTGTCATGCT TGAAGCCCCT CGTTTTATAA CCACTCATGA TCATGGTACA GGTTGTACTA TTTCAGCTGC CATCGCAGCT TTGTTGCCTA CAAAGCCTTT GGTTGATGCG GTTAAACAAG CAAAAGACTA TTTAAATGGG GCTTTATCTA CCTCAAGTGC TTTACAAGTA GGGAAAGGAC GTGGTCCCCT TCACCATTTT TATGAATTAT GGAAGAATGT A

Details for BaheA.01386.a.A1.GE26712

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKLQVSQDT LFIPRILSIA GTDPSGGAGM QADLKVFSAM KTYGMSVVTA VVAQNTQGVR AFQALDASFV ADQIDAVFED IHVDAVKIGM VANAQIARTV AERLAYHKAR FIVFDPVMVA KSGDVLLKSD AIEIXXDVLV PMSTLITPNL PEASLLLGCE VKWSLDAMYQ YGPQLLTLGC HAVLLKGGHL DVFSRTNEAN YDYSSPDLYC DCEDLVMLEA PRFITTHDHG TGCTISAAIA ALLPTKPLVD AVKQAKXYLX GALSTSSALQ VGKGRGPLHH FYELWK

Validated NT Sequence

tcttccataa ttcataaaaa tggtgaaggg gaccacgtcc tttccctact tgtaaagcac ttgaggtaga taaagccccn tttaaatant cttttgcttg tttaaccgca tcaaccaaag gctttgtagg caacaaagct gcgatggcag ctgaaatagt acaacctgta ccatgatcat gagtggttat aaaacgaggg gcttcaagca tgacaagatc ttcacaatca caataaagat ctggactaga ataatcataa ttcgcctcat ttgtacgaga aaaaacgtcc aaatgcccac ccttcaaaag aactgcatga caccctagag tcagaagctg tggaccatat tggtacatag cgtctaaaga ccactttacc tcacatccca acaatagaga tgcctcaggt aaatttggcg tgatcaaagt tgacatcggc acaagaacat cannnataat ttcaatcgca tcagacttta aaaggacatc gccacttttt gccaccataa cgggatcaaa aacaatgaag cgcgctttgt gataagcaag acgttctgcg acagtccgag caatttgagc atttgctacc ataccaattt taacagcatc aacatgaata tcttcaaaaa ccgcatcaat ttggtcagca acaaaagaag catccaacgc ctgaaaagca cgtacacctt gagtattttg tgcaaccaca gcggtcacca cactcattcc ataggttttc atagctgaaa aaactttcaa atccgcttgc attccagcgc caccagaagg atctgttccc gcaatagata aaatccgtgg aataaaaaga gtgtcttggc taacctgtaa tttcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatn gta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKLQVSQDT LFIPRILSIA GTDPSGGAGM QADLKVFSAM KTYGMSVVTA VVAQNTQGVR AFQALDASFV ADQIDAVFED IHVDAVKIGM VANAQIARTV AERLAYHKAR FIVFDPVMVA KSGDVLLKSD AIEIMRDVLV PMSTLITPNL PEASLLLGCE VKWSLDAMYQ YGPQLLTLGC HAVLLKGGHL DVFSRTNEAN YDYSSPDLYC DCEDLVMLEA PRFITTHDHG TGCTISAAIA ALLPTKPLVD AVKQAKDYLN GALSTSSALQ VGKGRGPLHH FYELWKNV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaattacag gttagccaag acactctttt tattccacgg attttatcta ttgcgggaac agatccttct ggtggcgctg gaatgcaagc ggatttgaaa gttttttcag ctatgaaaac ctatggaatg agtgtggtga ccgctgtggt tgcacaaaat actcaaggtg tacgtgcttt tcaggcgttg gatgcttctt ttgttgctga ccaaattgat gcggtttttg aagatattca tgttgatgct gttaaaattg gtatggtagc aaatgctcaa attgctcgga ctgtcgcaga acgtcttgct tatcacaaag cgcgcttcat tgtttttgat cccgttatgg tggcaaaaag tggcgatgtc cttttaaagt ctgatgcgat tgaaattatg cgtgatgttc ttgtgccgat gtcaactttg atcacgccaa atttacctga ggcatctcta ttgttgggat gtgaggtaaa gtggtcttta gacgctatgt accaatatgg tccacagctt ctgactctag ggtgtcatgc agttcttttg aagggtgggc atttggacgt tttttctcgt acaaatgagg cgaattatga ttattctagt ccagatcttt attgtgattg tgaagatctt gtcatgcttg aagcccctcg ttttataacc actcatgatc atggtacagg ttgtactatt tcagctgcca tcgcagcttt gttgcctaca aagcctttgg ttgatgcggt taaacaagca aaagactatt taaatggggc tttatctacc tcaagtgctt tacaagtagg gaaaggacgt ggtccccttc accattttta tgaattatgg aagaatgtaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a