BaheA.01383.a

tyrosyl-tRNA synthetase

CENTER ID: BaheA.01383.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01383.a.MB1.GE30411 Full length( BaheA.01383.a ) 1 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.916
RefSeq: YP_033671.1
UniProt: Q6G3B6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLAFKSDFLN IMSERGFIHQ ISDEKGLDAL FSKEVVSAYI GFDPTASSLH AGSLLQIMML YWLQKTGHRP IVLMGGGTGL IGDPSFKDEA RRLLTQDDIA ANIADIKKVF ARYLTFGERE TDSCIVNNAE WLCTLNYLEF LRDIGKHFSV NRMLSFDSVR LRLEREHSLS FLEFNYMILQ AYDFVELYKR YGLRMQMGGS DQWGNIINGI ELGHRLGTPQ LYALTSPLLT TSSGAKMGKS LNGAVWLNAD MLSPYQFWQY WRNTEDADVT RFLKLYTPLP MDEILKLSAL QGTEINEAKK ILATEITAML HGRDLANAVA ETARKTFEER TLGENLPTFE INANDLKMGT GLLILLVQAG LSKSNSEARR HIQGGGVRVN DHIIEDETHL IREEDINAQG IIKLSFGKKK HVLIKPL
NT Sequence
GTGCTTGCCT TTAAATCTGA TTTTTTAAAC ATTATGAGCG AACGTGGTTT TATCCACCAA ATTTCCGATG AAAAAGGCTT AGATGCTCTT TTTTCAAAAG AAGTTGTGAG CGCTTATATT GGATTTGATC CTACAGCATC AAGTCTCCAT GCTGGCAGTC TTCTTCAAAT TATGATGCTT TATTGGTTAC AAAAAACCGG TCATCGACCC ATTGTTTTGA TGGGAGGAGG AACAGGATTA ATCGGTGATC CTTCCTTTAA GGATGAAGCG AGACGTCTTC TGACACAAGA TGATATTGCT GCAAACATTG CTGATATTAA AAAGGTATTT GCTCGATATT TAACATTCGG TGAGAGAGAA ACCGATTCAT GCATAGTCAA TAATGCTGAA TGGCTGTGCA CCTTAAACTA CTTAGAATTT TTGCGTGATA TAGGAAAGCA TTTTTCCGTT AATCGTATGT TGTCATTTGA TTCTGTCAGA CTCAGACTTG AACGCGAGCA TTCTTTATCA TTCTTAGAGT TTAATTATAT GATCCTACAA GCTTATGATT TTGTTGAACT TTATAAGCGT TATGGGCTCC GTATGCAAAT GGGTGGCTCT GATCAATGGG GCAATATTAT TAACGGAATT GAACTAGGAC ATCGCTTAGG AACACCACAA TTATATGCTC TCACTTCACC ATTACTGACA ACGTCTTCCG GTGCAAAAAT GGGTAAGTCA TTAAATGGAG CTGTCTGGCT TAATGCTGAT ATGCTGTCAC CTTATCAATT TTGGCAATAT TGGCGAAACA CAGAAGATGC TGATGTTACA CGATTTTTAA AGCTTTATAC ACCATTGCCA ATGGACGAGA TTCTTAAACT TTCTGCATTA CAAGGGACTG AAATTAATGA AGCAAAGAAA ATTCTTGCAA CAGAAATTAC AGCAATGTTA CACGGACGCG ATCTTGCAAA TGCAGTAGCA GAAACTGCCC GTAAAACTTT TGAAGAAAGA ACTCTCGGAG AAAATCTTCC AACTTTTGAA ATTAACGCCA ATGATTTAAA AATGGGTACT GGATTGCTGA TTCTTTTAGT ACAAGCAGGA CTTTCTAAAT CGAACAGTGA AGCACGCCGA CATATTCAAG GTGGAGGAGT TCGTGTCAAT GATCACATTA TTGAGGATGA AACACATCTT ATTCGCGAAG AAGATATCAA TGCACAAGGA ATCATTAAAC TTTCTTTCGG TAAGAAAAAA CATGTTTTGA TAAAACCACT C
Details for BaheA.01383.a.MB1.GE30411
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 92kDa
OBSERVED MW: 89kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 39
Validated AA Sequence
MVLAFKSDFL NIMSERGFIH QISDEKGLDA LFSKEVVSAY IGFDPTASSL HAGSLLQIMM LYWLQKTGHR PIVLMGGGTG LIGDPSFKDE ARRLLTQDDI AANIADIKKV FARYLTFGER ETDSCIVNNA EWLCTLNYLE FLRDIGKHFS VNRMLSFDSV RLRLEREHSL SFLEFNYMIL QAYDFVELYK RYGLRMXMGG SDQWGNIING IELGHRLGTP QLYALTSPLL TTSXGAKMGK SLNGAVWLNA DMLSPYQFWQ YWRNTEXADV TRFLKLYTPL XXDEILKLSA LXGLKXMKXX KXLXXXXAMX XXXDXXXQXX KXXVXF
Validated NT Sequence
ggnnctcaga nccngggtcc tggttcgatg gtgcttgcct ttaaatctga ttttttaaac attatgagcg aacgtggttt tatccaccaa atttccgatg aaaaaggctt agatgctctt ttttcaaaag aagttgtgag cgcttatatt ggatttgatc ctacagcatc aagtctccat gctggcagtc ttcttcaaat tatgatgctt tattggttac aaaaaaccgg tcatcgaccc attgttttga tgggaggagg aacaggatta atcggtgatc cttcctttaa ggatgaagcg agacgtcttc tgacacaaga tgatattgct gcaaacattg ctgatattaa aaaggtattt gctcgatatt taacattcgg tgagagagaa accgattcat gcatagtcaa taatgctgaa tggctgtgca ccttaaacta cttagaattt ttgcgtgata taggaaagca tttttccgtt aatcgtatgt tgtcatttga ttctgtcaga ctcagacttg aacgcgagca ttctttatca ttcttagagt ttaattatat gatcctacaa gcttatgatt ttgttgaact ttataagcgt tatgggctcc gtatgcnaat gggtggctct gatcaatggg gcaatattat taacggnatt gaactaggac atcgcttagg aacaccacaa ttatatgctc tcacttcacc attactgaca acgtcttncg gtgcaaaaat gggtaagtca ttaaatggag ctgtctggct taatgctgat atgctgtcac cttatcaatt ttggcaatat tggcgaaaca cagaagangc tgatgttaca cgatttttaa agctttatac accattgcnn anggacgaga ttcttaaact ttctgcatta cnnggnctga aatnnatgaa gcnangaaaa tncttgnana nnnnnncagc aatgttncnc nnnngcgatn tnnnanngca gnancnnaaa ntgcncgtaa anttttgann nnnnnnnnnn nnaaannnnn nncttttnnn nnnnnnnnna ntnnnnannn nncnggnnng ctgannnnnt nnannngnnt tnntnannnn nnnnnnnnnn nncnnnnnnt nnnnnnnnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnnnanannn nnnnnnnnnn nnnnannc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MVLAFKSDFL NIMSERGFIH QISDEKGLDA LFSKEVVSAY IGFDPTASSL HAGSLLQIMM LYWLQKTGHR PIVLMGGGTG LIGDPSFKDE ARRLLTQDDI AANIADIKKV FARYLTFGER ETDSCIVNNA EWLCTLNYLE FLRDIGKHFS VNRMLSFDSV RLRLEREHSL SFLEFNYMIL QAYDFVELYK RYGLRMQMGG SDQWGNIING IELGHRLGTP QLYALTSPLL TTSSGAKMGK SLNGAVWLNA DMLSPYQFWQ YWRNTEDADV TRFLKLYTPL PMDEILKLSA LQGTEINEAK KILATEITAM LHGRDLANAV AETARKTFEE RTLGENLPTF EINANDLKMG TGLLILLVQA GLSKSNSEAR RHIQGGGVRV NDHIIEDETH LIREEDINAQ GIIKLSFGKK KHVLIKPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggt gcttgccttt aaatctgatt ttttaaacat tatgagcgaa cgtggtttta tccaccaaat ttccgatgaa aaaggcttag atgctctttt ttcaaaagaa gttgtgagcg cttatattgg atttgatcct acagcatcaa gtctccatgc tggcagtctt cttcaaatta tgatgcttta ttggttacaa aaaaccggtc atcgacccat tgttttgatg ggaggaggaa caggattaat cggtgatcct tcctttaagg atgaagcgag acgtcttctg acacaagatg atattgctgc aaacattgct gatattaaaa aggtatttgc tcgatattta acattcggtg agagagaaac cgattcatgc atagtcaata atgctgaatg gctgtgcacc ttaaactact tagaattttt gcgtgatata ggaaagcatt tttccgttaa tcgtatgttg tcatttgatt ctgtcagact cagacttgaa cgcgagcatt ctttatcatt cttagagttt aattatatga tcctacaagc ttatgatttt gttgaacttt ataagcgtta tgggctccgt atgcaaatgg gtggctctga tcaatggggc aatattatta acggaattga actaggacat cgcttaggaa caccacaatt atatgctctc acttcaccat tactgacaac gtcttccggt gcaaaaatgg gtaagtcatt aaatggagct gtctggctta atgctgatat gctgtcacct tatcaatttt ggcaatattg gcgaaacaca gaagatgctg atgttacacg atttttaaag ctttatacac cattgccaat ggacgagatt cttaaacttt ctgcattaca agggactgaa attaatgaag caaagaaaat tcttgcaaca gaaattacag caatgttaca cggacgcgat cttgcaaatg cagtagcaga aactgcccgt aaaacttttg aagaaagaac tctcggagaa aatcttccaa cttttgaaat taacgccaat gatttaaaaa tgggtactgg attgctgatt cttttagtac aagcaggact ttctaaatcg aacagtgaag cacgccgaca tattcaaggt ggaggagttc gtgtcaatga tcacattatt gaggatgaaa cacatcttat tcgcgaagaa gatatcaatg cacaaggaat cattaaactt tctttcggta agaaaaaaca tgttttgata aaaccactct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa