BaheA.01351.a

S-adenosyl-methyltransferase MraW

CENTER ID: BaheA.01351.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01351.a.MB1.GE30410 Full length( BaheA.01351.a ) 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1182
RefSeq: YP_033904.1
UniProt: Q6G2P7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIDLKKHKS RRIILTKQGK RTERHIPVLL QPVLAGLIPL VGAKVIDGTF GAGGYTRALL NAGAQVIALD RDPHAIREGQ SLVDEFFPRL RLVQGNFSQL DCVVEEKVDA VILDIGVSSM QLDEAERGFS FQKDGPLDMR MAQTGFTAAD VVNRLKSDEL ARIFKILGEE RYARRIARMI EKRRCVQPFL RTGDLAHAIE VLVGRKSGER IHPATRVFQA LRIYVNDEIG ELARGLFAAE RVLKPGGRLG VVSFHSLEDR MVKRFFSARS GESVRSRYLP EIEMAPATFF PLFKGGITAN KEELERNPRS RSARLRIGVR TEAECLFADM KLFGLAKIAS FEGGKK
NT Sequence
ATGGCTATTG ATTTAAAAAA GCATAAATCA AGACGGATTA TTTTGACAAA ACAGGGTAAG AGAACTGAGC GCCATATTCC AGTGTTGTTA CAGCCAGTTT TAGCTGGGCT TATACCATTG GTTGGGGCAA AAGTGATTGA TGGCACCTTT GGTGCTGGTG GTTATACACG TGCTTTGTTA AATGCGGGTG CACAGGTTAT TGCTCTTGAT CGTGATCCTC ACGCTATTCG TGAAGGACAA TCGCTTGTTG ATGAATTTTT TCCACGACTT CGTTTGGTGC AAGGGAATTT TTCACAGTTG GATTGTGTGG TTGAAGAGAA GGTAGATGCC GTTATTTTGG ATATTGGTGT CTCTTCAATG CAGCTTGATG AAGCTGAAAG AGGATTTTCT TTTCAAAAAG ATGGTCCATT AGATATGCGA ATGGCTCAGA CTGGTTTTAC TGCTGCTGAT GTTGTGAATC GTTTAAAAAG TGATGAGTTA GCGCGAATCT TTAAAATATT AGGAGAAGAA CGCTATGCAA GGCGAATTGC ACGGATGATT GAAAAGCGCC GCTGTGTTCA ACCGTTTTTG CGTACAGGTG ATCTTGCTCA TGCCATTGAG GTGTTGGTGG GGCGTAAAAG CGGAGAGCGC ATTCATCCTG CAACGCGTGT ATTTCAGGCT CTTCGCATTT ATGTGAACGA TGAAATTGGT GAACTTGCGC GTGGCTTATT TGCTGCTGAA CGCGTTTTGA AACCAGGAGG TCGTTTGGGC GTCGTGAGTT TTCATTCTCT TGAAGATCGT ATGGTTAAAA GATTTTTTTC TGCTCGTTCA GGAGAAAGTG TGAGATCTCG CTATCTTCCT GAAATAGAGA TGGCTCCAGC AACATTTTTT CCTTTATTTA AAGGGGGGAT AACTGCAAAT AAAGAGGAGT TGGAGCGAAA TCCTCGTTCA CGTTCTGCGA GGTTGCGTAT TGGTGTACGT ACTGAGGCTG AGTGTCTTTT TGCAGATATG AAATTGTTTG GGTTAGCAAA GATTGCCAGT TTTGAAGGTG GCAAGAAA
Details for BaheA.01351.a.MB1.GE30410
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 83kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 29
Validated AA Sequence
MAIDLKKHKS RRIILTKQGK RTERHIPVLL QPVLAGLIPL VGAKVIDGTF GAGGYTRALL NAGAQVTALD RDPHAIREGQ SLVDEFFPRL RLVQGNFSQL DCVVEEKVDA VILDIGVSSM QLDEAERGFS FQKDGPLDMR MAQTGFTAAD VVNRLKSDEL ARIFKILGEE RYARRIARMI EKRXCVQPFL RTGDLAHAIE VLVGRKXGER IHPATRVFXX FAXM
Validated NT Sequence
ggnnctcaga cccagggtcc tggttcgatg gctattgatt taaaaaagca taaatcaaga cggattattt tgacaaaaca gggtaagaga actgagcgcc atattccagt gttgttacag ccagttttag ctgggcttat accattggtt ggggcaaaag tgattgatgg cacctttggt gctggtggtt atacacgtgc tttgttaaat gcgggtgcac aggttactgc tcttgatcgt gatcctcacg ctattcgtga aggacaatcg cttgttgatg aattttttcc acgacttcgt ttggtgcaag ggaatttttc acagttggat tgtgtggttg aagagaaggt agatgccgtt attttggata ttggtgtctc ttcaatgcag cttgatgaag ctgaaagagg attttctttt caaaaagatg gtccattaga tatgcgaatg gctcagactg gttttactgc tgctgatgtt gtgaatcgnt taaaaagtga tgagttagcg cgaatcttta aaatattagg agaagaacgc tatgcaaggc gaattgcacg gatgattgaa aagcgccnct gtgttcaacc gtttttgcgt acaggtgatc ttgctcatgc cattgaggtg ttggtggggc gtaaaancgg agagcgcatt catccngcaa cgcgtgtatt tcnnntcttc gcanttatgt gancnangaa attggtgaac ntgcgcgtgg ctnanttgct gctgaacncg ttttgnnacc nnganncgtt tgggcgtcgt gagttttcat tctcttgaag nnnnnanggn tnanngantt ttttnnngct cnntcannan aaagtgtgag ancnnnnnnn cttncnnaaa tnnnnatngc tccngcnnnn ntttttnncn ttntttnnnn nnnggnnnnt gnnnnnnnnn naannnnncn ntnannttnt nnnnngnnnn nnnnnntgta cnnnnnnnnn nngnnngncn ntnnnnnnnn nnnannngnt nngntnnnnn nnnnntnnnn nnncnnnnnn nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nna
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MAIDLKKHKS RRIILTKQGK RTERHIPVLL QPVLAGLIPL VGAKVIDGTF GAGGYTRALL NAGAQVIALD RDPHAIREGQ SLVDEFFPRL RLVQGNFSQL DCVVEEKVDA VILDIGVSSM QLDEAERGFS FQKDGPLDMR MAQTGFTAAD VVNRLKSDEL ARIFKILGEE RYARRIARMI EKRRCVQPFL RTGDLAHAIE VLVGRKSGER IHPATRVFQA LRIYVNDEIG ELARGLFAAE RVLKPGGRLG VVSFHSLEDR MVKRFFSARS GESVRSRYLP EIEMAPATFF PLFKGGITAN KEELERNPRS RSARLRIGVR TEAECLFADM KLFGLAKIAS FEGGKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc tattgattta aaaaagcata aatcaagacg gattattttg acaaaacagg gtaagagaac tgagcgccat attccagtgt tgttacagcc agttttagct gggcttatac cattggttgg ggcaaaagtg attgatggca cctttggtgc tggtggttat acacgtgctt tgttaaatgc gggtgcacag gttattgctc ttgatcgtga tcctcacgct attcgtgaag gacaatcgct tgttgatgaa ttttttccac gacttcgttt ggtgcaaggg aatttttcac agttggattg tgtggttgaa gagaaggtag atgccgttat tttggatatt ggtgtctctt caatgcagct tgatgaagct gaaagaggat tttcttttca aaaagatggt ccattagata tgcgaatggc tcagactggt tttactgctg ctgatgttgt gaatcgttta aaaagtgatg agttagcgcg aatctttaaa atattaggag aagaacgcta tgcaaggcga attgcacgga tgattgaaaa gcgccgctgt gttcaaccgt ttttgcgtac aggtgatctt gctcatgcca ttgaggtgtt ggtggggcgt aaaagcggag agcgcattca tcctgcaacg cgtgtatttc aggctcttcg catttatgtg aacgatgaaa ttggtgaact tgcgcgtggc ttatttgctg ctgaacgcgt tttgaaacca ggaggtcgtt tgggcgtcgt gagttttcat tctcttgaag atcgtatggt taaaagattt ttttctgctc gttcaggaga aagtgtgaga tctcgctatc ttcctgaaat agagatggct ccagcaacat tttttccttt atttaaaggg gggataactg caaataaaga ggagttggag cgaaatcctc gttcacgttc tgcgaggttg cgtattggtg tacgtactga ggctgagtgt ctttttgcag atatgaaatt gtttgggtta gcaaagattg ccagttttga aggtggcaag aaataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa