SSGCID: Target Page

BaheA.01340.a

dihydrolipoamide dehydrogenase

CENTER ID:	BaheA.01340.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01340.a.A1.GE26705	Full length( BaheA.01340.a )		1	486

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01340.a.A1.PS00327	Full length( BaheA.01340.a )		1	486

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.633
RefSeq:	YP_033412.1
UniProt:	A0A0H3LWB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MANLYDVIVI GSGPGGYVTA IRAAQCGFKT AIVEREHLGG ICLNWGCIPT KALLRSAEIK HFSEHLKDYG LKLNGSIEAD IKDVVARSRA VSARLNAGVG FLMKKNKIDI IWGEAKFTKK AKGSQLVEIT VSSSSKPIMQ PQNPVPKGTL GEGIYQAKHI IIATGARPRI LPGIEPDGKL IWTYFEAMIP HVMPKSLLVM GSGAIGIEFA SFYHDMGASV TVVEMMPHIM PAEDIEISTF ARKRLEKKGI RILSQAKVTK VEKAVDSITA HIDVKGKRET ITADRLISAV GVQGNIENLG LEALGIKTDR GCIVTDEWSW TGVEGIYAIG DVAGPPMLAH KAEEEGVICI ERLARLENIH PLDKRKIPGC TYCTPQVASV GLSEAAAKEA GYDIRVGRYS FSANGKAIAL GEDQGLVKTI FDKKTGQLLG AHMVGAEVTE LIQGFVIAMN LETTEEELMH TVFPHPTLSE MMKESVLDAY GQVLNA

NT Sequence

GTGGCGAATC TTTATGATGT AATTGTGATT GGATCAGGTC CTGGCGGATA TGTAACCGCA ATTCGTGCGG CACAATGTGG CTTTAAGACT GCGATTGTTG AACGTGAACA TCTAGGTGGG ATTTGCTTAA ATTGGGGTTG TATTCCAACA AAGGCACTTT TACGTTCAGC GGAAATAAAG CATTTTTCTG AACATTTGAA GGATTATGGT TTAAAGCTCA ACGGTTCAAT TGAGGCTGAT ATCAAAGATG TCGTGGCACG TTCGCGTGCA GTTTCAGCGC GTTTAAATGC TGGTGTTGGT TTTTTGATGA AAAAAAACAA AATCGATATT ATTTGGGGTG AAGCGAAATT TACAAAGAAG GCGAAAGGGA GTCAGCTTGT GGAAATTACG GTTTCGTCAT CTTCCAAACC GATTATGCAA CCGCAAAATC CAGTACCAAA AGGAACATTA GGAGAAGGGA TTTATCAAGC AAAGCACATC ATTATTGCAA CGGGTGCACG TCCTCGTATC CTTCCTGGTA TTGAGCCAGA TGGGAAGCTC ATTTGGACTT ATTTTGAAGC TATGATTCCC CACGTAATGC CGAAATCGCT CTTGGTCATG GGATCTGGGG CCATTGGCAT TGAATTTGCT TCTTTTTATC ATGATATGGG GGCAAGCGTA ACTGTTGTTG AAATGATGCC TCACATCATG CCAGCTGAGG ATATTGAAAT TTCAACATTT GCTCGTAAAC GGTTAGAGAA AAAAGGCATT CGCATTCTTA GCCAAGCAAA AGTGACAAAG GTTGAAAAAG CTGTCGATTC CATTACTGCA CATATTGATG TGAAGGGTAA AAGAGAAACG ATCACAGCGG ATCGATTGAT TTCCGCTGTT GGGGTTCAGG GTAATATTGA GAATCTTGGA TTAGAAGCAT TAGGTATAAA AACTGATCGT GGATGCATTG TAACTGATGA GTGGAGTTGG ACAGGTGTAG AGGGTATTTA TGCGATTGGT GATGTCGCTG GTCCACCTAT GTTAGCACAT AAAGCAGAAG AAGAAGGTGT AATATGCATT GAACGTCTTG CGAGATTGGA GAATATTCAC CCTCTTGATA AAAGGAAAAT TCCAGGCTGT ACATATTGTA CACCGCAAGT CGCCTCTGTA GGGCTTTCAG AAGCGGCGGC AAAAGAAGCA GGCTATGATA TACGTGTTGG TCGTTATTCC TTTTCAGCGA ATGGCAAAGC GATTGCTTTG GGTGAAGATC AGGGATTAGT GAAAACTATT TTTGATAAAA AAACAGGACA GCTTCTTGGT GCGCATATGG TAGGGGCAGA AGTAACAGAA TTGATTCAAG GTTTTGTTAT TGCTATGAAT CTTGAAACAA CCGAGGAAGA ATTGATGCAT ACTGTCTTTC CACATCCGAC ATTATCGGAA ATGATGAAAG AGAGTGTGTT GGATGCTTAT GGTCAAGTTT TAAATGCT

Details for BaheA.01340.a.A1.GE26705

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	98

Validated AA Sequence

MXTLEAQTQG PGSMXXNLYD XIXIGSGPGG YXTAIRAAQC GFKTAIVERE HLXGICLNWG CIPTKALLXS XXIKHXSEHL KDYGLKLXGS IEADIKXVVX RSRAVSARLN AGVGFLMKXN KIDIIWGEAK FTKKAKGSQL VEITVSSSSK PIMQPQNPXP KGTLXEXIYX AKHIIIATGA RPRILPGIEP DGKLXWTYFX XMXPHVXXXX LXVMXXXXXX XEXASFYXXX XXSXXXXXMM PXIMXXXXIX ISXFARKRXX XKXXXXLAKX KXQXXXXLSI XXXHXLMXKG XRETITADRL IXXVXXXXNI ENLGLEALGI KTDRGXIVTD EWSWTGVEGI YAIGDVAGPP MLAHKAEEEG VICIERXARL ENIHPLDKRK IPGCTYCTPQ VASVGLSEAA AKEAGYDIRV GRYSFSXNGK AIALGEXQGL VKXIFDKKTG QLLGAHMVGA EVTELIRGFV IAMNLETTEE ELMHTVFPHP TLSEMMKESV LDAYGQVL

Validated NT Sequence

ttaaaacttg accataagca tccaacacac tctctttcat catttccgat aatgtcggat gtggaaagac agtatgcatc aattcttcct cggttgtttc aagattcata gcaataacaa aacctcgaat caattctgtt acttctgccc ctaccatatg cgcaccaaga agctgtcctg tttttttatc aaaaatantt ttcactaatc cctgntcttc acccaaagca atcgctttgc cattcnctga aaaggaataa cgaccaacac gtatatcata gcctgcttct tttgccgccg cttctgaaag ccctacagag gcgacttgcg gtgtacaata tgtacagcct ggaattttcc ttttatcaag agggtgaata ttctccaatc tcgcangacg ttcaatgcat attacacctt cttcttctgc tttatgtgct aacataggtg gaccagcgac atcaccaatc gcataaatac cctctacacc tgtccaactc cactcatcag ttacaatgna tccacgatca gtttttatac ctaatgcttc taatccaaga ttctcaatat tancnngaan ccnaacnnnn nnaatcaatc gatccgctgt gatcgtttct ctttnnccct tnnacatcaa tangtgnngn nnngnaatcg acagnttttn nnnnntttgn cactttnnct tggctaanan nnnnnntgnc tttntncnnn nncgtttacg agcaaannnt gaaattnnaa tnncntnann nngcatgatn ngaggcatca tttnnnnnnc agnnncgctn nnnnccannn nnngataaaa agaagcnant tcnnnnncnn tgnnccnnna nnccatgacc angagcgnnn tngncnntac gtggggantc atannntcaa aataagtcca anngagcttn ccatctggct caatnccagg aaggatacga ggacgtgcac ccgttgcaat aatgatgtgc tttgcntgat aaatccnttc tcntaatgtt ccttttggta ntggattttg cggttgcata atcggtttgg aagatgacga aaccgtaatt tccacaagct gactcccttt cgccttcttt gtaaatttcg cttcncccca aataatatcg attttgtttt ntttcatcaa aaaaccaaca ccagcattta ancgcgcnga aactgcacgc gaacgnnnca cgacancttt gatatcagcc tcaattgaac cgntgagctt taanccataa tccttcaaat gttcaganaa atgctttatt tncnctgann ntaaaagngc ctttgttgga atacaacccc aatttaagca aatcccnnct agatgttcac gttcaacaat cgcagtctta aagccacatt gngccgcacg aattgcggtt ncatatccgc caggncctga tccaatcnca attnnatcat aaagattngn cnccatcgaa ccaggaccct gggtctgagc ttccagggtn nncatatgat ggtgatggtg atgagcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVANLYDVI VIGSGPGGYV TAIRAAQCGF KTAIVEREHL GGICLNWGCI PTKALLRSAE IKHFSEHLKD YGLKLNGSIE ADIKDVVARS RAVSARLNAG VGFLMKKNKI DIIWGEAKFT KKAKGSQLVE ITVSSSSKPI MQPQNPVPKG TLGEGIYQAK HIIIATGARP RILPGIEPDG KLIWTYFEAM IPHVMPKSLL VMGSGAIGIE FASFYHDMGA SVTVVEMMPH IMPAEDIEIS TFARKRLEKK GIRILSQAKV TKVEKAVDSI TAHIDVKGKR ETITADRLIS AVGVQGNIEN LGLEALGIKT DRGCIVTDEW SWTGVEGIYA IGDVAGPPML AHKAEEEGVI CIERLARLEN IHPLDKRKIP GCTYCTPQVA SVGLSEAAAK EAGYDIRVGR YSFSANGKAI ALGEDQGLVK TIFDKKTGQL LGAHMVGAEV TELIQGFVIA MNLETTEEEL MHTVFPHPTL SEMMKESVLD AYGQVLNA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgaat ctttatgatg taattgtgat tggatcaggt cctggcggat atgtaaccgc aattcgtgcg gcacaatgtg gctttaagac tgcgattgtt gaacgtgaac atctaggtgg gatttgctta aattggggtt gtattccaac aaaggcactt ttacgttcag cggaaataaa gcatttttct gaacatttga aggattatgg tttaaagctc aacggttcaa ttgaggctga tatcaaagat gtcgtggcac gttcgcgtgc agtttcagcg cgtttaaatg ctggtgttgg ttttttgatg aaaaaaaaca aaatcgatat tatttggggt gaagcgaaat ttacaaagaa ggcgaaaggg agtcagcttg tggaaattac ggtttcgtca tcttccaaac cgattatgca accgcaaaat ccagtaccaa aaggaacatt aggagaaggg atttatcaag caaagcacat cattattgca acgggtgcac gtcctcgtat ccttcctggt attgagccag atgggaagct catttggact tattttgaag ctatgattcc ccacgtaatg ccgaaatcgc tcttggtcat gggatctggg gccattggca ttgaatttgc ttctttttat catgatatgg gggcaagcgt aactgttgtt gaaatgatgc ctcacatcat gccagctgag gatattgaaa tttcaacatt tgctcgtaaa cggttagaga aaaaaggcat tcgcattctt agccaagcaa aagtgacaaa ggttgaaaaa gctgtcgatt ccattactgc acatattgat gtgaagggta aaagagaaac gatcacagcg gatcgattga tttccgctgt tggggttcag ggtaatattg agaatcttgg attagaagca ttaggtataa aaactgatcg tggatgcatt gtaactgatg agtggagttg gacaggtgta gagggtattt atgcgattgg tgatgtcgct ggtccaccta tgttagcaca taaagcagaa gaagaaggtg taatatgcat tgaacgtctt gcgagattgg agaatattca ccctcttgat aaaaggaaaa ttccaggctg tacatattgt acaccgcaag tcgcctctgt agggctttca gaagcggcgg caaaagaagc aggctatgat atacgtgttg gtcgttattc cttttcagcg aatggcaaag cgattgcttt gggtgaagat cagggattag tgaaaactat ttttgataaa aaaacaggac agcttcttgg tgcgcatatg gtaggggcag aagtaacaga attgattcaa ggttttgtta ttgctatgaa tcttgaaaca accgaggaag aattgatgca tactgtcttt ccacatccga cattatcgga aatgatgaaa gagagtgtgt tggatgctta tggtcaagtt ttaaatgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BaheA.01340.a.A1.PS00327

PURIFICATION DATe:	7/17/2009
CONCENTRATION:	58.02mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	100µl
PERCENT IDENTITY:	81
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCGAAT CTTTATGATG TAATTGTGAT TGGATCAGGT CCTGGCGGAT ATGTAACCGC AATTCGTGCG GCACAATGTG GCTTTAAGAC TGCGATTGTT GAACGTGAAC ATCTAGGTGG GATTTGCTTA AATTGGGGTT GTATTCCAAC AAAGGCACTT TTACGTTCAG CGGAAATAAA GCATTTTTCT GAACATTTGA AGGATTATGG TTTAAAGCTC AACGGTTCAA TTGAGGCTGA TATCAAAGAT GTCGTGGCAC GTTCGCGTGC AGTTTCAGCG CGTTTAAATG CTGGTGTTGG TTTTTTGATG AAAAAAAACA AAATCGATAT TATTTGGGGT GAAGCGAAAT TTACAAAGAA GGCGAAAGGG AGTCAGCTTG TGGAAATTAC GGTTTCGTCA TCTTCCAAAC CGATTATGCA ACCGCAAAAT CCAGTACCAA AAGGAACATT AGGAGAAGGG ATTTATCAAG CAAAGCACAT CATTATTGCA ACGGGTGCAC GTCCTCGTAT CCTTCCTGGT ATTGAGCCAG ATGGGAAGCT CATTTGGACT TATTTTGAAG CTATGATTCC CCACGTAATG CCGAAATCGC TCTTGGTCAT GGGATCTGGG GCCATTGGCA TTGAATTTGC TTCTTTTTAT CATGATATGG GGGCAAGCGT AACTGTTGTT GAAATGATGC CTCACATCAT GCCAGCTGAG GATATTGAAA TTTCAACATT TGCTCGTAAA CGGTTAGAGA AAAAAGGCAT TCGCATTCTT AGCCAAGCAA AAGTGACAAA GGTTGAAAAA GCTGTCGATT CCATTACTGC ACATATTGAT GTGAAGGGTA AAAGAGAAAC GATCACAGCG GATCGATTGA TTTCCGCTGT TGGGGTTCAG GGTAATATTG AGAATCTTGG ATTAGAAGCA TTAGGTATAA AAACTGATCG TGGATGCATT GTAACTGATG AGTGGAGTTG GACAGGTGTA GAGGGTATTT ATGCGATTGG TGATGTCGCT GGTCCACCTA TGTTAGCACA TAAAGCAGAA GAAGAAGGTG TAATATGCAT TGAACGTCTT GCGAGATTGG AGAATATTCA CCCTCTTGAT AAAAGGAAAA TTCCAGGCTG TACATATTGT ACACCGCAAG TCGCCTCTGT AGGGCTTTCA GAAGCGGCGG CAAAAGAAGC AGGCTATGAT ATACGTGTTG GTCGTTATTC CTTTTCAGCG AATGGCAAAG CGATTGCTTT GGGTGAAGAT CAGGGATTAG TGAAAACTAT TTTTGATAAA AAAACAGGAC AGCTTCTTGG TGCGCATATG GTAGGGGCAG AAGTAACAGA ATTGATTCAA GGTTTTGTTA TTGCTATGAA TCTTGAAACA ACCGAGGAAG AATTGATGCA TACTGTCTTT CCACATCCGA CATTATCGGA AATGATGAAA GAGAGTGTGT TGGATGCTTA TGGTCAAGTT TTAAATGCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A