SSGCID: Target Page

BaheA.01300.a

bifunctional riboflavin kinase/FMN adenylyltransferase

CENTER ID:	BaheA.01300.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01300.a.A1.GE26699	Full length( BaheA.01300.a )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1440
RefSeq:	YP_034048.1
UniProt:	A0A0H3LZG3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLPLVWKGAV LALGNFDGVH CGHQVVLQKA LDLARMRNKP ALVLTFEPHP RSFFQHSAPI DRLTEAPEKA EIFKILGFNG VIEQPFDAHF AALSADEFIN VILKKAFEVS VVVTGENFQF GRQKSGNSRL LCQKGEEWGF EVVQIPCVSS APNSQSVIIS SSVIRKLLSQ GQVEKAARLL GYHYRVRSNI IQGEQLGRLL GFPTANQILP PQASLAYGVY AVRLRRANGV LYDGVASFGC RPTVVKNAAP FLETYLFDFN GDLYGENCTV SFFQFLRKQE KFDGLEPLIA QMHHDKKKAK AILAAVQPLS QLDQLLTFKN RT

NT Sequence

ATGCTTCCTT TGGTTTGGAA AGGGGCGGTA TTGGCACTTG GAAATTTTGA TGGTGTGCAT TGTGGTCATC AGGTGGTACT TCAAAAAGCG CTCGATTTAG CACGCATGAG AAATAAGCCA GCTCTTGTTT TAACCTTTGA GCCGCATCCA AGAAGTTTTT TTCAACACTC AGCTCCCATT GATCGCTTAA CAGAGGCTCC TGAAAAAGCT GAAATTTTTA AAATCCTCGG CTTTAATGGT GTGATTGAAC AGCCTTTTGA TGCACATTTT GCTGCTCTTT CAGCAGATGA ATTTATCAAT GTGATTTTAA AAAAAGCTTT CGAGGTTTCG GTTGTGGTAA CGGGTGAAAA TTTTCAATTT GGTCGTCAGA AAAGTGGAAA TTCACGTCTT CTTTGTCAAA AGGGAGAAGA ATGGGGATTT GAAGTTGTGC AAATTCCTTG TGTTTCGAGC GCACCAAATT CACAATCAGT GATCATTTCC TCTAGTGTTA TTCGTAAGCT TCTCTCACAA GGTCAGGTGG AAAAAGCAGC CCGTTTATTG GGCTATCATT ATCGGGTGCG CTCAAATATT ATTCAGGGCG AACAACTTGG TCGCCTTCTA GGGTTTCCTA CTGCTAACCA GATTCTTCCT CCTCAAGCCA GTTTAGCGTA TGGTGTTTAT GCAGTACGAT TGCGTCGTGC TAACGGTGTT TTGTATGATG GAGTTGCAAG TTTTGGTTGT CGTCCAACAG TTGTGAAAAA TGCAGCACCG TTTCTAGAAA CCTATTTATT TGATTTTAAC GGTGACCTTT ATGGAGAAAA CTGCACTGTT TCTTTCTTCC AGTTTTTACG AAAACAAGAA AAATTTGATG GGCTTGAGCC TTTAATTGCC CAAATGCATC ATGACAAAAA AAAAGCAAAA GCCATTTTGG CGGCAGTACA GCCATTGTCG CAGCTTGATC AGCTGCTTAC TTTTAAAAAT AGGACT

Details for BaheA.01300.a.A1.GE26699

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMLPLXWK GAVLALGNFD GVHCGHQVVL QKALDLARMR NKPALVLTFE PHPRSFFQHS APIDRLTEAP EKAEIFKILG FNGVIEQPFD AHFAALSADE FINVILKKAF EVSVVVTGEN FQFGRQKSGN SRLLCQKGEE WGFEVVQIPC VSSAPNSQSV IISSSVIRKL LSQGQVEKAA RLLGYHYRVR SNIIQGEQLG RLLGFPTANQ ILPPQASLAY GVYAVRLRRA NGVLYDGVAS FGCRPTVVKX AAPFLETYLF DFNGDLYGEN CTVSFFQFLR KQEKFDGLEP LIAQMHHDKK KAKAILAAVQ PLSQLDQLLT FKNRT

Validated NT Sequence

attaagtcct atttttaaaa gtaagcagct gatcaagctg cgacaatggc tgtactgccg ccaaaatggc ttttgctttt tttttgtcat gatgcatttg ggcaattaaa ggctcaagcc catcaaattt ttcttgtttt cgtaaaaact ggaagaaaga aacagtgcag ttttctccat aaaggtcacc gttaaaatca aataaatagg tttctagaaa cggtgctgcn tttttcacaa ctgttggacg acaaccaaaa cttgcaactc catcatacaa aacaccgtta gcacgacgca atcgtactgc ataaacacca tacgctaaac tggcttgagg aggaagaatc tggttagcag taggaaaccc tagaaggcga ccaagttgtt cgccctgaat aatatttgag cgcacccgat aatgatagcc caataaacgg gctgcttttt ccacctgacc ttgtgagaga agcttacgaa taacactaga ggaaatgatc actgattgtg aatttggtgc gctcgaaaca caaggaattt gcacaacttc aaatccccat tcttctccct tttgacaaag aagacgtgaa tttccacttt tctgacgacc aaattgaaaa ttttcacccg ttaccacaac cgaaacctcg aaagcttttt ttaaaatcac attgataaat tcatctgctg aaagagcagc aaaatgtgca tcaaaaggct gttcaatcac accattaaag ccgaggattt taaaaatttc agctttttca ggagcctctg ttaagcgatc aatgggagct gagtgttgaa aaaaacttct tggatgcggc tcaaaggtta aaacaagagc tggcttattt ctcatgcgtg ctaaatcgag cgctttttga agtaccacct gatgaccaca atgcacacca tcaaaatttc caagtgccaa taccgcccct ttccaaanca aaggaagcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgan ggngangagc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLPLVWKGA VLALGNFDGV HCGHQVVLQK ALDLARMRNK PALVLTFEPH PRSFFQHSAP IDRLTEAPEK AEIFKILGFN GVIEQPFDAH FAALSADEFI NVILKKAFEV SVVVTGENFQ FGRQKSGNSR LLCQKGEEWG FEVVQIPCVS SAPNSQSVII SSSVIRKLLS QGQVEKAARL LGYHYRVRSN IIQGEQLGRL LGFPTANQIL PPQASLAYGV YAVRLRRANG VLYDGVASFG CRPTVVKNAA PFLETYLFDF NGDLYGENCT VSFFQFLRKQ EKFDGLEPLI AQMHHDKKKA KAILAAVQPL SQLDQLLTFK NRT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttcctttg gtttggaaag gggcggtatt ggcacttgga aattttgatg gtgtgcattg tggtcatcag gtggtacttc aaaaagcgct cgatttagca cgcatgagaa ataagccagc tcttgtttta acctttgagc cgcatccaag aagttttttt caacactcag ctcccattga tcgcttaaca gaggctcctg aaaaagctga aatttttaaa atcctcggct ttaatggtgt gattgaacag ccttttgatg cacattttgc tgctctttca gcagatgaat ttatcaatgt gattttaaaa aaagctttcg aggtttcggt tgtggtaacg ggtgaaaatt ttcaatttgg tcgtcagaaa agtggaaatt cacgtcttct ttgtcaaaag ggagaagaat ggggatttga agttgtgcaa attccttgtg tttcgagcgc accaaattca caatcagtga tcatttcctc tagtgttatt cgtaagcttc tctcacaagg tcaggtggaa aaagcagccc gtttattggg ctatcattat cgggtgcgct caaatattat tcagggcgaa caacttggtc gccttctagg gtttcctact gctaaccaga ttcttcctcc tcaagccagt ttagcgtatg gtgtttatgc agtacgattg cgtcgtgcta acggtgtttt gtatgatgga gttgcaagtt ttggttgtcg tccaacagtt gtgaaaaatg cagcaccgtt tctagaaacc tatttatttg attttaacgg tgacctttat ggagaaaact gcactgtttc tttcttccag tttttacgaa aacaagaaaa atttgatggg cttgagcctt taattgccca aatgcatcat gacaaaaaaa aagcaaaagc cattttggcg gcagtacagc cattgtcgca gcttgatcag ctgcttactt ttaaaaatag gactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa