BaheA.01272.a

Pyruvate dehydrogenase E1 component, alpha subunit

CENTER ID: BaheA.01272.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01272.a.B1.GE26937 Full length( BaheA.01272.a ) 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.630
RefSeq: YP_033409.1
UniProt: Q8L1Z6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAERLKKNSA SVVHNALSNT TKKAKIANFT KEEEIDAYRE MLLIRRFEEK AGQLYGMGLI GGFCHLYIGQ EAVVIGTLKA AKEGDQVITS YRDHGHMLAV GMSPRGVMAE LTGRQGGFSK GKGGSMHMFS KEKNFYGGHG IVGAQVPIGS GLAFSNQYLG KDNVTLVYFG DGAANQGQVY ESFNMASLWK LPVVYIIENN QYAMGTSVAR ASAETDFSRR GLSFEIPGIV VDGMDVRAVK GAADEAITWT RSGKGPIILD MQTYRYRGHS MSDPAKYRSK EEVQKIKEEQ DPIDQVRNRI LQQGFASEDD LKSIDKEVRA IVADAVDFAQ SDQEPDASEL YTDILV
NT Sequence
ATGGCAGAAC GATTAAAAAA AAATTCTGCG TCGGTGGTGC ATAATGCATT ATCAAACACC ACAAAGAAAG CAAAAATAGC TAATTTTACA AAAGAAGAGG AAATTGATGC CTACCGTGAG ATGCTTTTAA TTCGCCGTTT TGAAGAAAAA GCAGGCCAAC TTTATGGTAT GGGGCTTATC GGAGGATTTT GTCATCTCTA TATTGGTCAA GAAGCTGTTG TTATTGGAAC GCTGAAGGCA GCAAAAGAAG GTGATCAGGT TATTACATCT TACCGTGATC ACGGACACAT GTTGGCGGTT GGGATGAGTC CCCGTGGGGT TATGGCAGAG TTAACAGGGC GTCAAGGTGG TTTTTCTAAG GGGAAAGGGG GATCGATGCA TATGTTTTCT AAAGAAAAGA ATTTTTATGG TGGTCATGGC ATTGTTGGTG CACAGGTTCC GATTGGTTCT GGTTTGGCGT TTTCGAATCA GTATCTTGGT AAAGATAATG TAACATTGGT GTATTTTGGT GATGGTGCAG CCAATCAGGG GCAGGTTTAC GAAAGTTTTA ATATGGCTTC GCTTTGGAAG CTTCCTGTTG TCTATATTAT TGAAAATAAC CAGTATGCTA TGGGGACATC AGTTGCTCGT GCCTCTGCAG AAACTGATTT TTCTCGTCGT GGTCTTTCCT TTGAAATTCC AGGTATTGTT GTTGACGGTA TGGATGTTCG AGCAGTAAAA GGGGCTGCTG ATGAAGCAAT TACTTGGACA CGTTCAGGTA AAGGGCCTAT TATTCTTGAT ATGCAAACTT ATCGTTATCG TGGTCACTCC ATGTCTGATC CAGCGAAATA TCGCTCAAAG GAAGAAGTGC AGAAAATAAA AGAGGAACAG GATCCGATTG ATCAGGTAAG AAATCGAATT CTTCAACAAG GTTTTGCGAG CGAAGACGAT TTAAAGTCTA TTGATAAAGA GGTGCGTGCG ATTGTCGCTG ATGCGGTAGA TTTTGCGCAA AGTGATCAAG AGCCAGATGC TTCTGAGCTC TATACTGACA TTTTAGTT
Details for BaheA.01272.a.B1.GE26937
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/15/2009
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAERLKKNSA SVVHNALSNT TKKAKIANFT KEEEIDAYRE MLLIRRFEEK AGQLYGMGLI GGFCHLYIGQ EAVVIGTLKA AKEGDQVITS YRDHGHMLAV GMSPRGVMAE LTGRQGGFSK GKGGSMHMFS KEKNFYGGHG IVGAQVPIGS GLAFSNQYLG KDNVTLVYFG DGAANQGQVY ESFNMASLWK LPVVYIIENN QYAMGTSVAR ASAETDFSRR GLSFEIPGIV VDGMDVRAVK GAADEAITWT RSGKGPIILD MQTYRYRGHS MSDPAKYRSK EEVQKIKEEQ DPIDQVRNRI LQQGFASEDD LKSIDKEVRA IVADAVDFAQ SDQEPDASEL YTDI
Validated NT Sequence
aaatgtcagt atagagctca gaagcatctg gctcttgatc actttgcgca aaatctaccg catcagcgac aatcgcacgc acctctttat caatagactt taaatcgtct tcgctcgcaa aaccttgttg aagaattcga tttcttacct gatcaatcgg atcctgttcc tcttttattt tctgcacttc ttcctttgag cgatatttcg ctggatcaga catggagtga ccacgataac gataagtttg catatcaaga ataataggcc ctttacctga acgtgtccaa gtaattgctt catcagcagc cccttttact gctcgaacat ccataccgtc aacaacaata cctggaattt caaaggaaag accacgacga gaaaaatcag tttctgcaga ggcacgagca actgatgtcc ccatagcata ctggttattt tcaataatat agacaacagg aagcttccaa agcgaagcca tattaaaact ttcgtaaacc tgcccctgat tggctgcacc atcaccaaaa tacaccaatg ttacattatc tttaccaaga tactgattcg aaaacgccaa accagaacca atcggaacct gtgcaccaac aatgccatga ccaccataaa aattcttttc tttagaaaac atatgcatcg atcccccttt ccccttagaa aaaccacctt gacgccctgt taactctgcc ataaccccac ggggactcat cccaaccgcc aacatgtgtc cgtgatcacg gtaagatgta ataacctgat caccttcttt tgctgccttc agcgttccaa taacaacagc ttcttgacca atatagagat gacaaaatcc tccgataagc cccataccat aaagttggcc tgctttttct tcaaaacggc gaattaaaag catctcacgg taggcatcaa tttcctcttc ttttgtaaaa ttagctattt ttgctttctt tgtggtgttt gataatgcat tatgcaccac cgacgcagaa ttttttttta atcgttctgc catatggtgg tggtggtggt gagcc
Expected Protein Sequence
MAHHHHHHMA ERLKKNSASV VHNALSNTTK KAKIANFTKE EEIDAYREML LIRRFEEKAG QLYGMGLIGG FCHLYIGQEA VVIGTLKAAK EGDQVITSYR DHGHMLAVGM SPRGVMAELT GRQGGFSKGK GGSMHMFSKE KNFYGGHGIV GAQVPIGSGL AFSNQYLGKD NVTLVYFGDG AANQGQVYES FNMASLWKLP VVYIIENNQY AMGTSVARAS AETDFSRRGL SFEIPGIVVD GMDVRAVKGA ADEAITWTRS GKGPIILDMQ TYRYRGHSMS DPAKYRSKEE VQKIKEEQDP IDQVRNRILQ QGFASEDDLK SIDKEVRAIV ADAVDFAQSD QEPDASELYT DILV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag aacgattaaa aaaaaattct gcgtcggtgg tgcataatgc attatcaaac accacaaaga aagcaaaaat agctaatttt acaaaagaag aggaaattga tgcctaccgt gagatgcttt taattcgccg ttttgaagaa aaagcaggcc aactttatgg tatggggctt atcggaggat tttgtcatct ctatattggt caagaagctg ttgttattgg aacgctgaag gcagcaaaag aaggtgatca ggttattaca tcttaccgtg atcacggaca catgttggcg gttgggatga gtccccgtgg ggttatggca gagttaacag ggcgtcaagg tggtttttct aaggggaaag ggggatcgat gcatatgttt tctaaagaaa agaattttta tggtggtcat ggcattgttg gtgcacaggt tccgattggt tctggtttgg cgttttcgaa tcagtatctt ggtaaagata atgtaacatt ggtgtatttt ggtgatggtg cagccaatca ggggcaggtt tacgaaagtt ttaatatggc ttcgctttgg aagcttcctg ttgtctatat tattgaaaat aaccagtatg ctatggggac atcagttgct cgtgcctctg cagaaactga tttttctcgt cgtggtcttt cctttgaaat tccaggtatt gttgttgacg gtatggatgt tcgagcagta aaaggggctg ctgatgaagc aattacttgg acacgttcag gtaaagggcc tattattctt gatatgcaaa cttatcgtta tcgtggtcac tccatgtctg atccagcgaa atatcgctca aaggaagaag tgcagaaaat aaaagaggaa caggatccga ttgatcaggt aagaaatcga attcttcaac aaggttttgc gagcgaagac gatttaaagt ctattgataa agaggtgcgt gcgattgtcg ctgatgcggt agattttgcg caaagtgatc aagagccaga tgcttctgag ctctatactg acattttagt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat