BaheA.01267.a

2-keto-3-deoxy-6-phosphogluconate aldolase

CENTER ID:	BaheA.01267.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01267.a.A1.GE26696	Full length( BaheA.01267.a )		1	221

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132952
PATRIC ID:	fig\|283166.5.peg.1349
RefSeq:	YP_034017.1
UniProt:	Q8L204

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCQKIEKLLS LLQEQTIIPV LHIDTLQNAV PLARALVKGG LRTIEVTLRT ANALDAIKAI TQEVPESIVG AGTILSTIHY KQAEHAGAKF IVSPGFSNKL IDYAKNSEIP LLPSALTPSE VMKALDKGYS YLKFFPAEAA GGITFVKALA APFSEIRFCP TGGIKQKNAA QWLQLSNVFC IGGSWIAPRN LITAKNWHAI SALAQTAAQL SSYPTKVCST I

NT Sequence

ATGTGCCAAA AAATAGAGAA GCTGTTATCA CTGCTCCAGG AACAAACCAT TATACCTGTT TTGCATATTG ATACTCTACA AAATGCCGTG CCTTTAGCAC GTGCCCTTGT CAAAGGAGGC TTAAGAACAA TTGAAGTCAC CTTGCGAACA GCAAATGCAC TCGACGCGAT TAAAGCCATT ACACAAGAAG TACCCGAATC AATCGTTGGA GCAGGAACAA TTCTTAGCAC TATACACTAT AAACAAGCAG AACATGCAGG AGCAAAATTT ATCGTAAGTC CTGGATTTTC AAACAAATTG ATTGATTATG CAAAAAACAG TGAAATCCCC CTTCTCCCAA GTGCTCTTAC ACCAAGTGAA GTAATGAAAG CACTGGACAA AGGCTATTCA TATCTTAAAT TTTTTCCTGC TGAAGCAGCA GGAGGTATTA CCTTTGTAAA AGCTTTAGCC GCCCCCTTCT CTGAAATCCG ATTTTGCCCG ACAGGCGGCA TAAAACAAAA AAATGCTGCT CAATGGCTCC AGCTTTCTAA TGTCTTCTGT ATTGGTGGTT CTTGGATAGC TCCTCGAAAT CTTATCACAG CAAAAAATTG GCATGCGATT AGCGCATTAG CACAAACTGC AGCACAACTT TCCTCTTATC CTACAAAAGT GTGTTCCACA ATA

Details for BaheA.01267.a.A1.GE26696

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMCQKIEKLL SLLQEQTIIP VLHIDTLQNA VPLARALVKG GLRTIEVTLR TANALDAIKA ITQEVPESIV GAGTILSTIH YKQAEHAGAK FIVSPGFSNK LIDYAKNSEI PLLPSALTPS EVMKALDKGY SYLKFFPAEA AGGITFVKAL AAPFSEIRFC PTGGIKQKNA AQWLQLSNVF CIGGSWIAPR NLITAKNWHA ISALAQTAAQ LSSYPTKVCS TI

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtgcc aaaaaataga gaagctgtta tcactgctcc aggaacaaac cattatacct gttttgcata ttgatactct acaaaatgcc gtgcctttag cacgtgccct tgtcaaagga ggcttaagaa caattgaagt caccttgcga acagcaaatg cactcgacgc gattaaagcc attacacaag aagtacccga atcaatcgtt ggagcaggaa caattcttag cactatacac tataaacaag cagaacatgc aggagcaaaa tttatcgtaa gtcctggatt ttcaaacaaa ttgattgatt atgcaaaaaa cagtgaaatc ccccttctcc caagtgctct tacaccaagt gaagtaatga aagcactgga caaaggctat tcatatctta aattttttcc tgctgaagca gcaggaggta ttacctttgt aaaagcttta gccgccccct tctctgaaat ccgattttgc ccgacaggcg gcataaaaca aaaaaatgct gctcaatggc tccagctttc taatgtcttc tgtattggtg gttcttggat agctcctcga aatcttatca cagcaaaaaa ttggcatgcg attagcgcat tagcacaaac tgcagcacaa ctttcctctt atcctacaaa agtgtgttcc acaatataat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgccaaaaa atagagaagc tgttatcact gctccaggaa caaaccatta tacctgtttt gcatattgat actctacaaa atgccgtgcc tttagcacgt gcccttgtca aaggaggctt aagaacaatt gaagtcacct tgcgaacagc aaatgcactc gacgcgatta aagccattac acaagaagta cccgaatcaa tcgttggagc aggaacaatt cttagcacta tacactataa acaagcagaa catgcaggag caaaatttat cgtaagtcct ggattttcaa acaaattgat tgattatgca aaaaacagtg aaatccccct tctcccaagt gctcttacac caagtgaagt aatgaaagca ctggacaaag gctattcata tcttaaattt tttcctgctg aagcagcagg aggtattacc tttgtaaaag ctttagccgc ccccttctct gaaatccgat tttgcccgac aggcggcata aaacaaaaaa atgctgctca atggctccag ctttctaatg tcttctgtat tggtggttct tggatagctc ctcgaaatct tatcacagca aaaaattggc atgcgattag cgcattagca caaactgcag cacaactttc ctcttatcct acaaaagtgt gttccacaat aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa