BaheA.01246.a
triosephosphate isomerase
| CENTER ID: | BaheA.01246.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.01246.a.A1.GE26693 | Full length( BaheA.01246.a ) | 1 | 254 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.01246.a.A1.PS00381 | Full length( BaheA.01246.a ) | 1 | 254 |
Structures
3KXQ
DEPOSITED: 12/3/2009
DETERMINATION: XRay
CLONE: BaheA.01246.a.A1.GE26693
PROTEIN: BaheA.01246.a.A1.PS00381
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127002 |
| PATRIC ID: | fig|283166.5.peg.622 |
| RefSeq: | YP_033402.1 |
| UniProt: | Q8L1Z5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPNIRPFIA GNWKMNGTGE SLGELRAIAA GISSDLGRLF EALICVPATL LSRAFDILGG ENILLGGQNC HFDDYGPYTG DISAFMLKEA GASHVIIGHS ERRTVYQESD AIVRAKVQAA WRAGLVALIC VGETLEERKS NKVLDVLTRQ LEGSLPDGAT AENIIIAYEP VWAVGTGNTA TSADVAEVHA FIHHKMHSRF GDEGAKIRLL YGGSVKPSNA FELLSTAHVN GALIGGASLK AIDFLTICDV YRKL
NT Sequence
ATGTCTCCAA ATATTCGACC TTTTATTGCG GGAAATTGGA AAATGAATGG AACTGGTGAA TCTCTTGGAG AATTACGTGC CATTGCCGCT GGCATTAGCT CTGATTTAGG TCGTTTGTTT GAAGCGCTTA TTTGTGTTCC AGCAACGCTT TTATCCCGTG CTTTTGATAT TCTGGGTGGA GAAAATATTC TCTTAGGAGG GCAAAATTGT CATTTTGATG ATTATGGTCC TTATACTGGA GATATTTCAG CTTTTATGCT TAAAGAAGCA GGAGCAAGTC ATGTCATTAT TGGGCATTCA GAGCGTCGCA CAGTTTATCA GGAAAGTGAT GCAATTGTTC GTGCTAAAGT GCAAGCGGCA TGGCGAGCAG GTCTTGTTGC TCTTATTTGT GTTGGAGAAA CATTGGAAGA ACGCAAAAGT AATAAAGTGT TGGATGTGCT TACGCGGCAA CTTGAAGGAT CTTTACCAGA TGGTGCAACA GCAGAAAATA TCATTATCGC TTATGAACCT GTATGGGCTG TAGGGACAGG CAATACAGCG ACTTCAGCAG ATGTTGCAGA GGTACATGCG TTTATTCACC ATAAGATGCA TTCTCGTTTT GGTGATGAGG GGGCCAAGAT ACGTTTGCTG TATGGTGGAT CAGTAAAACC ATCCAATGCA TTCGAGCTTC TGAGTACTGC TCATGTGAAT GGTGCTCTGA TAGGTGGAGC AAGTTTAAAA GCGATTGATT TTTTAACTAT TTGCGATGTT TATCGTAAAT TA
Details for BaheA.01246.a.A1.GE26693
| HARVESTED ON: | 5/15/2009 |
| SEQUENCED ON: | 6/18/2009 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSPNIRPFI AGNWKMNGTG ESLGELRAIA AGISSDLGRL FEALICVPAT LLSRAFDILG GENILLGGQN CHFDDYGPYT GDISAFMLKE AGASHVIIGH SERRTVYQES DAIVRAKVQA AWRAGLVALI CVGETLEERK SNKVLDVLTR QLEGSLPDGA TAENIIIAYE PVWAVGTGNT ATSADVAEVH AFIHHKMHSR FGDEGAKIRL LYGGSVKPSN AFELLSTAHV NGALIGGASL KAIDFLTICD VYR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctc caaatattcg accttttatt gcgggaaatt ggaaaatgaa tggaactggt gaatctcttg gagaattacg tgccattgcc gctggcatta gctctgattt aggtcgtttg tttgaagcgc ttatttgtgt tccagcaacg cttttatccc gtgcttttga tattctgggt ggagaaaata ttctcttagg agggcaaaat tgtcattttg atgattatgg tccttatact ggagatattt cagcttttat gcttaaagaa gcaggagcaa gtcatgtcat tattgggcat tcagagcgtc gcacagttta tcaggaaagt gatgcaattg ttcgtgctaa agtgcaagcg gcatggcgag caggtcttgt tgctcttatt tgtgttggag aaacattgga agaacgcaaa agtaataaag tgttggatgt gcttacgcgg caacttgaag gatctttacc agatggtgca acagcagaaa atatcattat cgcttatgaa cctgtatggg ctgtagggac aggcaataca gcgacttcag cagatgttgc agaggtacat gcgtttattc accataagat gcattctcgt tttggtgatg agggggccaa gatacgtttg ctgtatggtg gatcagtaaa accatccaat gcattcgagc ttctgagtac tgctcatgtg aatggtgctc tgataggtgg agcaagttta aaagcgattg attttctaac tatttgcgat gtttatcgta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSPNIRPFI AGNWKMNGTG ESLGELRAIA AGISSDLGRL FEALICVPAT LLSRAFDILG GENILLGGQN CHFDDYGPYT GDISAFMLKE AGASHVIIGH SERRTVYQES DAIVRAKVQA AWRAGLVALI CVGETLEERK SNKVLDVLTR QLEGSLPDGA TAENIIIAYE PVWAVGTGNT ATSADVAEVH AFIHHKMHSR FGDEGAKIRL LYGGSVKPSN AFELLSTAHV NGALIGGASL KAIDFLTICD VYRKL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctccaaat attcgacctt ttattgcggg aaattggaaa atgaatggaa ctggtgaatc tcttggagaa ttacgtgcca ttgccgctgg cattagctct gatttaggtc gtttgtttga agcgcttatt tgtgttccag caacgctttt atcccgtgct tttgatattc tgggtggaga aaatattctc ttaggagggc aaaattgtca ttttgatgat tatggtcctt atactggaga tatttcagct tttatgctta aagaagcagg agcaagtcat gtcattattg ggcattcaga gcgtcgcaca gtttatcagg aaagtgatgc aattgttcgt gctaaagtgc aagcggcatg gcgagcaggt cttgttgctc ttatttgtgt tggagaaaca ttggaagaac gcaaaagtaa taaagtgttg gatgtgctta cgcggcaact tgaaggatct ttaccagatg gtgcaacagc agaaaatatc attatcgctt atgaacctgt atgggctgta gggacaggca atacagcgac ttcagcagat gttgcagagg tacatgcgtt tattcaccat aagatgcatt ctcgttttgg tgatgagggg gccaagatac gtttgctgta tggtggatca gtaaaaccat ccaatgcatt cgagcttctg agtactgctc atgtgaatgg tgctctgata ggtggagcaa gtttaaaagc gattgatttt ttaactattt gcgatgttta tcgtaaatta aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BaheA.01246.a.A1.PS00381
| PURIFICATION DATe: | 10/7/2009 |
| CONCENTRATION: | 21.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSPNIRPFI AGNWKMNGTG ESLGELRAIA AGISSDLGRL FEALICVPAT LLSRAFDILG GENILLGGQN CHFDDYGPYT GDISAFMLKE AGASHVIIGH SERRTVYQES DAIVRAKVQA AWRAGLVALI CVGETLEERK SNKVLDVLTR QLEGSLPDGA TAENIIIAYE PVWAVGTGNT ATSADVAEVH AFIHHKMHSR FGDEGAKIRL LYGGSVKPSN AFELLSTAHV NGALIGGASL KAIDFLTICD VYR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctc caaatattcg accttttatt gcgggaaatt ggaaaatgaa tggaactggt gaatctcttg gagaattacg tgccattgcc gctggcatta gctctgattt aggtcgtttg tttgaagcgc ttatttgtgt tccagcaacg cttttatccc gtgcttttga tattctgggt ggagaaaata ttctcttagg agggcaaaat tgtcattttg atgattatgg tccttatact ggagatattt cagcttttat gcttaaagaa gcaggagcaa gtcatgtcat tattgggcat tcagagcgtc gcacagttta tcaggaaagt gatgcaattg ttcgtgctaa agtgcaagcg gcatggcgag caggtcttgt tgctcttatt tgtgttggag aaacattgga agaacgcaaa agtaataaag tgttggatgt gcttacgcgg caacttgaag gatctttacc agatggtgca acagcagaaa atatcattat cgcttatgaa cctgtatggg ctgtagggac aggcaataca gcgacttcag cagatgttgc agaggtacat gcgtttattc accataagat gcattctcgt tttggtgatg agggggccaa gatacgtttg ctgtatggtg gatcagtaaa accatccaat gcattcgagc ttctgagtac tgctcatgtg aatggtgctc tgataggtgg agcaagttta aaagcgattg attttctaac tatttgcgat gtttatcgta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSPNIRPFI AGNWKMNGTG ESLGELRAIA AGISSDLGRL FEALICVPAT LLSRAFDILG GENILLGGQN CHFDDYGPYT GDISAFMLKE AGASHVIIGH SERRTVYQES DAIVRAKVQA AWRAGLVALI CVGETLEERK SNKVLDVLTR QLEGSLPDGA TAENIIIAYE PVWAVGTGNT ATSADVAEVH AFIHHKMHSR FGDEGAKIRL LYGGSVKPSN AFELLSTAHV NGALIGGASL KAIDFLTICD VYRKL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTCCAAAT ATTCGACCTT TTATTGCGGG AAATTGGAAA ATGAATGGAA CTGGTGAATC TCTTGGAGAA TTACGTGCCA TTGCCGCTGG CATTAGCTCT GATTTAGGTC GTTTGTTTGA AGCGCTTATT TGTGTTCCAG CAACGCTTTT ATCCCGTGCT TTTGATATTC TGGGTGGAGA AAATATTCTC TTAGGAGGGC AAAATTGTCA TTTTGATGAT TATGGTCCTT ATACTGGAGA TATTTCAGCT TTTATGCTTA AAGAAGCAGG AGCAAGTCAT GTCATTATTG GGCATTCAGA GCGTCGCACA GTTTATCAGG AAAGTGATGC AATTGTTCGT GCTAAAGTGC AAGCGGCATG GCGAGCAGGT CTTGTTGCTC TTATTTGTGT TGGAGAAACA TTGGAAGAAC GCAAAAGTAA TAAAGTGTTG GATGTGCTTA CGCGGCAACT TGAAGGATCT TTACCAGATG GTGCAACAGC AGAAAATATC ATTATCGCTT ATGAACCTGT ATGGGCTGTA GGGACAGGCA ATACAGCGAC TTCAGCAGAT GTTGCAGAGG TACATGCGTT TATTCACCAT AAGATGCATT CTCGTTTTGG TGATGAGGGG GCCAAGATAC GTTTGCTGTA TGGTGGATCA GTAAAACCAT CCAATGCATT CGAGCTTCTG AGTACTGCTC ATGTGAATGG TGCTCTGATA GGTGGAGCAA GTTTAAAAGC GATTGATTTT TTAACTATTT GCGATGTTTA TCGTAAATTA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA