SSGCID: Target Page

BaheA.01219.a

Ferredoxin-NADP reductase

CENTER ID:	BaheA.01219.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01219.a.A1.GE26691	Full length( BaheA.01219.a )		1	276

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1353
RefSeq:	YP_034018.1
UniProt:	A0A0H3M401

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTSASNVQS NISSVASDFP IPENVFALTV QEVYHYTDRL FKFRLNRPES FRFRSGEFVM IGLPNAEKPI YRAYSIASPF WDEQLEFFSI KVPGGPLTEH LQKIKIGDTV LMRKKSTGTL VLDALIPGKR LYLLSTGTGV APFASLIRDP ETYEKFSEVV LIQTTRECDE LNYAKDLVVS LQQDPLIGEY APQLKFYPMT TRESSEHMGR ITTVMESGAF FEMTGLPKIH ADEDRVMICG SMAMLKDCAR MCEVFGLVEG ANNAPATYVV ERAFVG

NT Sequence

ATGAATACGT CTGCTAGCAA TGTTCAGTCA AACATCAGCA GCGTTGCATC AGATTTTCCT ATTCCTGAAA ATGTATTCGC GCTTACTGTA CAGGAAGTTT ATCACTATAC AGACCGTTTG TTTAAATTTC GCTTGAATCG TCCTGAAAGT TTTCGTTTTC GTTCTGGTGA ATTTGTTATG ATTGGTTTGC CAAATGCAGA AAAGCCAATT TATCGTGCTT ATTCGATTGC AAGTCCATTT TGGGATGAGC AGCTTGAATT TTTTTCGATC AAAGTTCCAG GAGGGCCATT AACCGAACAT CTCCAAAAAA TTAAAATTGG TGATACCGTT CTTATGCGTA AAAAATCTAC TGGAACGCTG GTTCTTGATG CCCTTATTCC TGGAAAACGC CTTTATCTTT TGTCAACAGG GACAGGAGTT GCTCCCTTTG CAAGTCTTAT CCGTGATCCT GAAACTTATG AAAAATTTTC AGAAGTCGTT CTTATTCAAA CAACTCGTGA GTGCGATGAA CTTAATTATG CAAAAGATTT GGTTGTCTCT CTACAACAAG ATCCACTAAT TGGTGAATAC GCACCACAGC TGAAATTTTA TCCTATGACT ACGCGCGAAT CTTCTGAGCA TATGGGGCGC ATTACGACTG TTATGGAGAG TGGCGCTTTT TTTGAAATGA CTGGTTTGCC GAAGATTCAT GCTGATGAAG ATCGGGTGAT GATTTGTGGT TCCATGGCGA TGCTGAAAGA TTGTGCGAGA ATGTGTGAAG TCTTTGGTCT TGTTGAAGGT GCCAATAATG CACCGGCTAC ATATGTTGTG GAGCGTGCCT TTGTAGGG

Details for BaheA.01219.a.A1.GE26691

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	75

Validated AA Sequence

MNTSASNVQS NIXSVASXFP IPENVFALTV QEVYHYTDRS FKFRLNRPES FRFRSGEXXM IGLPNAEKPI YRAYSIASPF WDEQLEXFSI KVPGGPLTEH LQKIKIGDTV LMRKKSTGTL VLDALIPGKR LYLLSTGTGV APFASLIRDP ETYEKFSEVV LIQTTRECDE LNYAKDLVVS LQQDPLIXEX APQLKFYPMT TRESSEHMXR ITTVMESGAF FEMT

Validated NT Sequence

cagtcatttc aaaaaaagcg ccactctcca taacagtngt aatgcgnnnc atatgctcag aagattcgcg ngtagtcata ggataaaatt tcagctgtgg tgcgnattcn ncaattagtg gatcttgttg tagagagaca accaaatctt ttgcataatt aagttcatcg cactcacgag ttgtttgaat aagaacgact tctgaaaatt tttcataagt ttcaggatca cggataagac ttgcaaaggg agcaactcct gtccctgttg acaaaagata aaggcgtttt ccaggaataa gggcatcaag aaccagcgtt ccagtagatt ttttacgcat aagaacggta tcaccaattt taattttttg gagatgttcg gttaatggcc ctcctggaac tttgatcgaa aaaanttcaa gctgctcatc ccaaaatgga ctngcaatng aataagcacg ataaattggc ttttctgcat ttggcaaacc aatcataann aattcaccag aacgaaaacg aaaactttca ggacgattca agcgaaattt aaacgaacgg tctgtatagt gataaacttc ctgtacagta agcgcgaata cattttcagg aataggaaan nntgatgcaa cgctgntgat gtttgactga acattgctag cagacgtatt catcgaacca ggaccctggg t

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNTSASNVQ SNISSVASDF PIPENVFALT VQEVYHYTDR LFKFRLNRPE SFRFRSGEFV MIGLPNAEKP IYRAYSIASP FWDEQLEFFS IKVPGGPLTE HLQKIKIGDT VLMRKKSTGT LVLDALIPGK RLYLLSTGTG VAPFASLIRD PETYEKFSEV VLIQTTRECD ELNYAKDLVV SLQQDPLIGE YAPQLKFYPM TTRESSEHMG RITTVMESGA FFEMTGLPKI HADEDRVMIC GSMAMLKDCA RMCEVFGLVE GANNAPATYV VERAFVG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatacgtct gctagcaatg ttcagtcaaa catcagcagc gttgcatcag attttcctat tcctgaaaat gtattcgcgc ttactgtaca ggaagtttat cactatacag accgtttgtt taaatttcgc ttgaatcgtc ctgaaagttt tcgttttcgt tctggtgaat ttgttatgat tggtttgcca aatgcagaaa agccaattta tcgtgcttat tcgattgcaa gtccattttg ggatgagcag cttgaatttt tttcgatcaa agttccagga gggccattaa ccgaacatct ccaaaaaatt aaaattggtg ataccgttct tatgcgtaaa aaatctactg gaacgctggt tcttgatgcc cttattcctg gaaaacgcct ttatcttttg tcaacaggga caggagttgc tccctttgca agtcttatcc gtgatcctga aacttatgaa aaattttcag aagtcgttct tattcaaaca actcgtgagt gcgatgaact taattatgca aaagatttgg ttgtctctct acaacaagat ccactaattg gtgaatacgc accacagctg aaattttatc ctatgactac gcgcgaatct tctgagcata tggggcgcat tacgactgtt atggagagtg gcgctttttt tgaaatgact ggtttgccga agattcatgc tgatgaagat cgggtgatga tttgtggttc catggcgatg ctgaaagatt gtgcgagaat gtgtgaagtc tttggtcttg ttgaaggtgc caataatgca ccggctacat atgttgtgga gcgtgccttt gtagggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa