SSGCID: Target Page

BaheA.01216.a

ABC transporter ATP-binding protein

CENTER ID:	BaheA.01216.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01216.a.A1.GE26690	Full length( BaheA.01216.a )		1	226

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.311
RefSeq:	YP_033127.1
UniProt:	Q6G4Q8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGIIKFDKVT QVFGDLYVLR NITVQLTERR IAVIGANGSG KSTFVRLING LQLPSHGFVS VDGLDTKNDA KAIKHKVGFV FQNPDNQIVL PLVEEDLSFG LKNLKLSKEE VKERVDEILQ RYDLQNFRNH AVHLLSGGQK QLVAISGVVA MKPDYIIFDE PTTLLDLRNK RLITQVIEEL SQTAIVVSHD LEFIRNFDRV LVFDKGEIVV DDIPLVAIKE YIRRMS

NT Sequence

TTGGGGATTA TAAAGTTTGA TAAGGTGACA CAAGTCTTTG GTGATTTGTA TGTGTTGAGG AATATTACCG TACAGCTTAC CGAAAGACGA ATCGCTGTTA TTGGTGCGAA TGGTTCTGGA AAAAGTACAT TTGTTCGTCT CATCAATGGG CTACAATTAC CCTCTCATGG TTTTGTGAGT GTTGATGGGC TTGATACCAA GAACGATGCA AAGGCAATAA AGCATAAAGT AGGATTTGTT TTCCAAAATC CTGATAATCA AATTGTTTTA CCATTAGTGG AAGAGGATTT ATCTTTTGGT CTCAAAAATC TAAAACTGAG TAAAGAGGAA GTTAAGGAGC GTGTAGATGA AATTTTACAG CGCTATGATC TTCAAAATTT TAGAAATCAC GCAGTGCATT TATTAAGTGG TGGACAAAAA CAACTCGTTG CGATTTCCGG TGTAGTCGCA ATGAAACCAG ACTATATTAT TTTTGATGAG CCAACAACAT TACTTGATTT GCGAAACAAG CGTCTCATTA CGCAAGTTAT AGAAGAATTA TCACAAACGG CAATTGTTGT ATCACATGAT TTGGAGTTTA TCAGAAACTT TGATAGAGTA TTAGTTTTTG ATAAGGGGGA GATAGTTGTT GATGATATAC CTTTGGTTGC TATCAAGGAA TATATAAGAA GAATGTCA

Details for BaheA.01216.a.A1.GE26690

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	69

Validated AA Sequence

MXTLEAQTQG PGSMLGIIKF DKVTQVFXDL YVLRNITVQL TERRIAVIGA NGSGKSTFVR LINGLQLPSH GFVSVDGLDT KNDAKAIKHX XXFVFXNPDN QIVLPLVEED LSFGLKNLKL SKXXVKERVD EILQRYDLQN FRNHAVHLLS GGQKQLXXIS GVXAMXPXYI IFD

Validated NT Sequence

catcaaaaat aatatantnn ggtntcattg cnnnnacacc ggaaatcncn ncgagttgtt tttgtccacc acttaataaa tgcactgcgt gatttctaaa attttgaaga tcatagcgct gtaaaatttc atctacacgc tccttaactn nntctttact cagttttaga tttttgagac caaaagataa atcctcttcc actaatggta aaacaatttg attatcagga ttttngaaaa caaatnntnn nttatgcttt attgcctttg catcgttctt ggtatcaagc ccatcaacac tcacaaaacc atgagagggt aattgtagcc cattgatgag acgaacaaat gtactttttc cagaaccatt cgcaccaata acagcgattc gtctttcggt aagctgtacg gtaatattcc tcaacacata caaatcanca aagacttgtg tcaccttatc aaactttata atccccaaca tcgaaccagg accctgggtc tgagcttcca gggtanccat atgatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLGIIKFDK VTQVFGDLYV LRNITVQLTE RRIAVIGANG SGKSTFVRLI NGLQLPSHGF VSVDGLDTKN DAKAIKHKVG FVFQNPDNQI VLPLVEEDLS FGLKNLKLSK EEVKERVDEI LQRYDLQNFR NHAVHLLSGG QKQLVAISGV VAMKPDYIIF DEPTTLLDLR NKRLITQVIE ELSQTAIVVS HDLEFIRNFD RVLVFDKGEI VVDDIPLVAI KEYIRRMS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggggatt ataaagtttg ataaggtgac acaagtcttt ggtgatttgt atgtgttgag gaatattacc gtacagctta ccgaaagacg aatcgctgtt attggtgcga atggttctgg aaaaagtaca tttgttcgtc tcatcaatgg gctacaatta ccctctcatg gttttgtgag tgttgatggg cttgatacca agaacgatgc aaaggcaata aagcataaag taggatttgt tttccaaaat cctgataatc aaattgtttt accattagtg gaagaggatt tatcttttgg tctcaaaaat ctaaaactga gtaaagagga agttaaggag cgtgtagatg aaattttaca gcgctatgat cttcaaaatt ttagaaatca cgcagtgcat ttattaagtg gtggacaaaa acaactcgtt gcgatttccg gtgtagtcgc aatgaaacca gactatatta tttttgatga gccaacaaca ttacttgatt tgcgaaacaa gcgtctcatt acgcaagtta tagaagaatt atcacaaacg gcaattgttg tatcacatga tttggagttt atcagaaact ttgatagagt attagttttt gataaggggg agatagttgt tgatgatata cctttggttg ctatcaagga atatataaga agaatgtcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a