SSGCID: Target Page

BaheA.01215.a

ribonucleotide-diphosphate reductase subunit beta

CENTER ID:	BaheA.01215.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01215.a.A1.GE26689	Full length( BaheA.01215.a )		1	324

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01215.a.A1.PW26763	Full length( BaheA.01215.a )		1	324

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.224
RefSeq:	YP_033048.1
UniProt:	A0A0H3LVI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKITTKNPV VCAVNWNRLH DEKDLEVWNR LTGNFWLPEK VPLSNDIPSW ASLTEEERKL TIRVFTGLTL LDTIQNTVGA ISLMADAITE HEEAVLTNIA FMEAVHARSY SSIFSTLCST VEVDDAFRWS EENIHLQKKA RLVLERYEGN DPLKKKIAST LLESFLFYSG FYLPMYWASR AKLTNTADLI RLIIRDEAVH GYYIGYKFQL GFAKLDETKK KEVKDFAFNL LFDLYNIECK YTEDLYDALG LTEDVKIFLH YNANKALMNL GFEALFPSEV CRVNPAILAA LSPNSDENHD FFSGAGSSYV IGKAVATTDD DWEF

NT Sequence

ATGACAAAGA TTACAACTAA AAATCCTGTT GTGTGCGCTG TCAATTGGAA TAGATTGCAT GATGAGAAAG ATCTTGAGGT TTGGAATCGT TTAACGGGAA ATTTTTGGTT GCCTGAAAAG GTGCCGCTTT CCAATGATAT TCCTTCATGG GCAAGTCTAA CGGAAGAAGA ACGCAAGTTA ACCATTCGTG TTTTTACAGG GTTAACACTT CTGGATACGA TTCAAAATAC TGTAGGTGCT ATTTCGCTTA TGGCTGATGC GATAACAGAG CATGAGGAGG CAGTGTTAAC GAACATTGCT TTTATGGAAG CGGTTCATGC ACGTTCTTAT TCTTCTATTT TTTCAACATT GTGTTCGACG GTAGAAGTTG ATGATGCTTT TAGATGGTCA GAGGAAAATA TTCATTTACA AAAAAAAGCC AGATTAGTTC TTGAGCGTTA TGAAGGAAAT GACCCACTCA AGAAGAAGAT TGCCTCGACT TTACTTGAAA GCTTTTTATT TTATTCTGGT TTTTATTTAC CCATGTATTG GGCCAGCCGT GCTAAATTGA CCAATACAGC TGATCTCATT CGGCTTATCA TTCGTGATGA AGCAGTCCAT GGTTATTATA TAGGTTATAA ATTTCAATTA GGGTTTGCAA AACTTGATGA AACCAAAAAG AAGGAAGTTA AGGATTTTGC TTTTAACTTG CTCTTTGACC TTTATAATAT TGAATGTAAA TATACTGAAG ATCTTTATGA TGCACTTGGA TTGACAGAAG ATGTTAAAAT TTTTCTTCAT TATAATGCAA ACAAGGCTTT AATGAATTTA GGTTTTGAAG CTCTTTTCCC GTCTGAGGTT TGTCGTGTTA ATCCTGCTAT TTTAGCCGCT TTATCACCAA ACTCTGATGA AAATCATGAT TTTTTTTCAG GAGCTGGCTC TTCTTATGTT ATTGGCAAGG CGGTTGCTAC CACGGATGAT GATTGGGAGT TT

Details for BaheA.01215.a.A1.GE26689

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTKITTK NPVVCAVNWN RLHDEKDLEV WNRLTGNFWL PEKVPLSNDI PSWASLTEEE RKLTIRVFTG LTLLDTIQNT VGAISLMADA ITEHEEAVLT NIAFMEAVHA RSYSSIFSTL CSTVEVDDAF RWSEENIHLQ KKARLVLERY EGNDPLKKKI ASTLLESFLF YSGFYLPMYW ASRAKLTNTA DLIRLIIRDE AVHGYYIGYK FQLGFAKLDE TKKKEVKDFA FNLLFDLYNI ECKYTEDLYD ALGLTEDVKI FLHYNANKAL MNLGFEALFP SEVCRVNPAI LAALSPNSDE NHDFFSGAGS SYVIGKAVAT TDDDW

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacaaa gattacaact aaaaatcctg ttgtgtgcgc tgtcaattgg aatagattgc atgatgagaa agatcttgag gtttggaatc gtttaacggg aaatttttgg ttgcctgaaa aggtgccgct ttccaatgat attccttcat gggcaagtct aacggaagaa gaacgcaagt taaccattcg tgtttttaca gggttaacac ttctggatac gattcaaaat actgtaggtg ctatttcgct tatggctgat gcgataacag agcatgagga ggcagtgtta acgaacattg cttttatgga agcggttcat gcacgttctt attcttctat tttttcaaca ttgtgttcga cggtagaagt tgatgatgct tttagatggt cagaggaaaa tattcattta caaaaaaaag ccagattagt tcttgagcgt tatgaaggaa atgacccact caagaagaag attgcctcga ctttacttga aagcttttta ttttattctg gtttttattt acccatgtat tgggccagcc gtgctaaatt gaccaataca gctgatctca ttcggcttat cattcgtgat gaagcagtcc atggttatta tataggttat aaatttcaat tagggtttgc aaaacttgat gaaaccaaaa agaaggaagt taaggatttt gcttttaact tgctctttga cctttataat attgaatgta aatatactga agatctttat gatgcacttg gattgacaga agatgttaaa atttttcttc attataatgc aaacaaggct ttaatgaatt taggttttga agctcttttc ccgtctgagg tttgtcgtgt taatcctgct attttagccg ctttatcacc aaactctgat gaaaatcatg attttttttc aggagctggc tcttcttatg ttattggcaa ggcggttgct accacggatg atgattggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTKITTKNP VVCAVNWNRL HDEKDLEVWN RLTGNFWLPE KVPLSNDIPS WASLTEEERK LTIRVFTGLT LLDTIQNTVG AISLMADAIT EHEEAVLTNI AFMEAVHARS YSSIFSTLCS TVEVDDAFRW SEENIHLQKK ARLVLERYEG NDPLKKKIAS TLLESFLFYS GFYLPMYWAS RAKLTNTADL IRLIIRDEAV HGYYIGYKFQ LGFAKLDETK KKEVKDFAFN LLFDLYNIEC KYTEDLYDAL GLTEDVKIFL HYNANKALMN LGFEALFPSE VCRVNPAILA ALSPNSDENH DFFSGAGSSY VIGKAVATTD DDWEF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaagatt acaactaaaa atcctgttgt gtgcgctgtc aattggaata gattgcatga tgagaaagat cttgaggttt ggaatcgttt aacgggaaat ttttggttgc ctgaaaaggt gccgctttcc aatgatattc cttcatgggc aagtctaacg gaagaagaac gcaagttaac cattcgtgtt tttacagggt taacacttct ggatacgatt caaaatactg taggtgctat ttcgcttatg gctgatgcga taacagagca tgaggaggca gtgttaacga acattgcttt tatggaagcg gttcatgcac gttcttattc ttctattttt tcaacattgt gttcgacggt agaagttgat gatgctttta gatggtcaga ggaaaatatt catttacaaa aaaaagccag attagttctt gagcgttatg aaggaaatga cccactcaag aagaagattg cctcgacttt acttgaaagc tttttatttt attctggttt ttatttaccc atgtattggg ccagccgtgc taaattgacc aatacagctg atctcattcg gcttatcatt cgtgatgaag cagtccatgg ttattatata ggttataaat ttcaattagg gtttgcaaaa cttgatgaaa ccaaaaagaa ggaagttaag gattttgctt ttaacttgct ctttgacctt tataatattg aatgtaaata tactgaagat ctttatgatg cacttggatt gacagaagat gttaaaattt ttcttcatta taatgcaaac aaggctttaa tgaatttagg ttttgaagct cttttcccgt ctgaggtttg tcgtgttaat cctgctattt tagccgcttt atcaccaaac tctgatgaaa atcatgattt tttttcagga gctggctctt cttatgttat tggcaaggcg gttgctacca cggatgatga ttgggagttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BaheA.01215.a.A1.PW26763

PURIFICATION DATe:	7/7/2009
CONCENTRATION:	21.51mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	32
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAAAGATT ACAACTAAAA ATCCTGTTGT GTGCGCTGTC AATTGGAATA GATTGCATGA TGAGAAAGAT CTTGAGGTTT GGAATCGTTT AACGGGAAAT TTTTGGTTGC CTGAAAAGGT GCCGCTTTCC AATGATATTC CTTCATGGGC AAGTCTAACG GAAGAAGAAC GCAAGTTAAC CATTCGTGTT TTTACAGGGT TAACACTTCT GGATACGATT CAAAATACTG TAGGTGCTAT TTCGCTTATG GCTGATGCGA TAACAGAGCA TGAGGAGGCA GTGTTAACGA ACATTGCTTT TATGGAAGCG GTTCATGCAC GTTCTTATTC TTCTATTTTT TCAACATTGT GTTCGACGGT AGAAGTTGAT GATGCTTTTA GATGGTCAGA GGAAAATATT CATTTACAAA AAAAAGCCAG ATTAGTTCTT GAGCGTTATG AAGGAAATGA CCCACTCAAG AAGAAGATTG CCTCGACTTT ACTTGAAAGC TTTTTATTTT ATTCTGGTTT TTATTTACCC ATGTATTGGG CCAGCCGTGC TAAATTGACC AATACAGCTG ATCTCATTCG GCTTATCATT CGTGATGAAG CAGTCCATGG TTATTATATA GGTTATAAAT TTCAATTAGG GTTTGCAAAA CTTGATGAAA CCAAAAAGAA GGAAGTTAAG GATTTTGCTT TTAACTTGCT CTTTGACCTT TATAATATTG AATGTAAATA TACTGAAGAT CTTTATGATG CACTTGGATT GACAGAAGAT GTTAAAATTT TTCTTCATTA TAATGCAAAC AAGGCTTTAA TGAATTTAGG TTTTGAAGCT CTTTTCCCGT CTGAGGTTTG TCGTGTTAAT CCTGCTATTT TAGCCGCTTT ATCACCAAAC TCTGATGAAA ATCATGATTT TTTTTCAGGA GCTGGCTCTT CTTATGTTAT TGGCAAGGCG GTTGCTACCA CGGATGATGA TTGGGAGTTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA