BaheA.01213.a

phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID: BaheA.01213.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01213.a.A1.GE26094 Full length( BaheA.01213.a ) 1 254
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01213.a.A1.PS00252 Full length( BaheA.01213.a ) 1 254

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1016
RefSeq: YP_033762.1
UniProt: Q6G338

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRRHRIYEG KAKILYEGPE PGTYIQFFKD DATAFNAKKH EIIDGKGVLN NRISEHIFSH LGRLGIPTHF IKRINMREQL IKAVEIIPLE VVVRNVAAGS LSKRLGLEEG TPLSQSIIEF YYKNDSLDDP MVSEEHITAF GWAVPQEIED IMQLSIRIND FLSGLFAGVN IQLIDFKMEF GRLWEDEAMR IVLADEISPD SARLWDIQTQ EKMDKDRFRR DMGGLINAYQ EVAKRLGIIN ENEPSRPNGP VLVK
NT Sequence
ATGAATCGTC GCCACCGTAT TTATGAAGGC AAGGCCAAAA TTCTGTATGA AGGACCTGAA CCAGGTACAT ATATTCAATT TTTTAAAGAT GACGCAACTG CTTTTAATGC AAAAAAGCAT GAAATTATCG ATGGAAAAGG AGTTTTAAAT AACCGTATCT CAGAACATAT CTTTAGCCAT CTTGGTCGTT TAGGCATCCC AACACACTTT ATCAAGCGTA TAAATATGCG CGAACAACTC ATTAAAGCTG TTGAAATTAT TCCCTTGGAG GTTGTTGTTC GTAACGTTGC TGCTGGTTCT CTTTCTAAGC GTTTGGGATT GGAAGAAGGT ACCCCTCTTT CGCAATCCAT CATTGAGTTT TATTATAAAA ACGATTCTCT TGATGATCCC ATGGTAAGCG AAGAACATAT CACTGCTTTT GGCTGGGCTG TTCCACAAGA AATAGAAGAC ATTATGCAGC TTTCAATTCG TATTAATGAT TTTCTTTCTG GACTTTTTGC AGGAGTTAAT ATTCAACTCA TTGATTTTAA AATGGAGTTT GGCCGCTTGT GGGAAGATGA AGCGATGCGT ATTGTTCTTG CCGATGAAAT ATCGCCTGAT TCTGCCCGAT TATGGGATAT ACAAACACAA GAAAAAATGG ATAAAGATCG TTTTCGGCGT GATATGGGAG GGCTTATTAA CGCCTATCAA GAAGTGGCAA AGCGTCTTGG TATTATTAAT GAAAATGAAC CTTCACGACC AAACGGTCCA GTTTTAGTGA AG
Details for BaheA.01213.a.A1.GE26094
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MNRRHRIYEG KAKILYEGPE PGTYIQFFKD DATAFNAKKH EIIDGKGVLN NRISEHIFSH LGRLGIPTHF IKRINMREQL IKAVEIIPLE VVVRNVAAGS LSKRLGLEEG TPLSQSIIEF YYKNDSLDDP MVSEEHITAF GWAVPQEIED IMQLSIRIND FLSGLFAGVN IQLIDFKMEF GRLWEDEAMR IVLADEISPD SARLWDIQTQ EKMDKDRFRR DMGGLINAYQ EVAKRLGIIN ENEPSRPNGP VLVK
Validated NT Sequence
gggncctggt tcgatgaatc gtcgccaccg tatttatgaa ggcaaggcca aaattctgta tgaaggacct gaaccaggta catatattca attttttaaa gatgacgcaa ctgcttttaa tgcaaaaaag catgaaatta tcgatggaaa aggagtttta aataaccgta tctcagaaca tatctttagc catcttggtc gtttaggcat cccaacacac tttatcaagc gtataaatat gcgcgaacaa ctcattaaag ctgttgaaat tattcccttg gaggttgttg ttcgtaacgt tgctgctggt tctctttcta agcgtttggg attggaagaa ggtacccctc tttcgcaatc catcattgag ttttattata aaaacgattc tcttgatgat cccatggtaa gcgaagaaca tatcactgct tttggctggg ctgttccaca agaaatagaa gacattatgc agctttcaat tcgtattaat gattttcttt ctggactttt tgcaggagtt aatattcaac tcattgattt taaaatggag tttggccgct tgtgggaaga tgaagcgatg cgtattgttc ttgccgatga aatatcgcct gattctgccc gattatggga tatacaaaca caagaaaaaa tggataaaga tcgttttcgg cgtgatatgg gagggcttat taacgcctat caagaagtgg caaagcgtct tggtattatt aatgaaaatg aaccttcacg accaaacggt ccagttttag tgaagtaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNRRHRIYE GKAKILYEGP EPGTYIQFFK DDATAFNAKK HEIIDGKGVL NNRISEHIFS HLGRLGIPTH FIKRINMREQ LIKAVEIIPL EVVVRNVAAG SLSKRLGLEE GTPLSQSIIE FYYKNDSLDD PMVSEEHITA FGWAVPQEIE DIMQLSIRIN DFLSGLFAGV NIQLIDFKME FGRLWEDEAM RIVLADEISP DSARLWDIQT QEKMDKDRFR RDMGGLINAY QEVAKRLGII NENEPSRPNG PVLVK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatcgtcgc caccgtattt atgaaggcaa ggccaaaatt ctgtatgaag gacctgaacc aggtacatat attcaatttt ttaaagatga cgcaactgct tttaatgcaa aaaagcatga aattatcgat ggaaaaggag ttttaaataa ccgtatctca gaacatatct ttagccatct tggtcgttta ggcatcccaa cacactttat caagcgtata aatatgcgcg aacaactcat taaagctgtt gaaattattc ccttggaggt tgttgttcgt aacgttgctg ctggttctct ttctaagcgt ttgggattgg aagaaggtac ccctctttcg caatccatca ttgagtttta ttataaaaac gattctcttg atgatcccat ggtaagcgaa gaacatatca ctgcttttgg ctgggctgtt ccacaagaaa tagaagacat tatgcagctt tcaattcgta ttaatgattt tctttctgga ctttttgcag gagttaatat tcaactcatt gattttaaaa tggagtttgg ccgcttgtgg gaagatgaag cgatgcgtat tgttcttgcc gatgaaatat cgcctgattc tgcccgatta tgggatatac aaacacaaga aaaaatggat aaagatcgtt ttcggcgtga tatgggaggg cttattaacg cctatcaaga agtggcaaag cgtcttggta ttattaatga aaatgaacct tcacgaccaa acggtccagt tttagtgaag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BaheA.01213.a.A1.PS00252
PURIFICATION DATe: 4/30/2009
CONCENTRATION: 1.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MNRRHRIYEG KAKILYEGPE PGTYIQFFKD DATAFNAKKH EIIDGKGVLN NRISEHIFSH LGRLGIPTHF IKRINMREQL IKAVEIIPLE VVVRNVAAGS LSKRLGLEEG TPLSQSIIEF YYKNDSLDDP MVSEEHITAF GWAVPQEIED IMQLSIRIND FLSGLFAGVN IQLIDFKMEF GRLWEDEAMR IVLADEISPD SARLWDIQTQ EKMDKDRFRR DMGGLINAYQ EVAKRLGIIN ENEPSRPNGP VLVK
Validated NT Sequence
gggncctggt tcgatgaatc gtcgccaccg tatttatgaa ggcaaggcca aaattctgta tgaaggacct gaaccaggta catatattca attttttaaa gatgacgcaa ctgcttttaa tgcaaaaaag catgaaatta tcgatggaaa aggagtttta aataaccgta tctcagaaca tatctttagc catcttggtc gtttaggcat cccaacacac tttatcaagc gtataaatat gcgcgaacaa ctcattaaag ctgttgaaat tattcccttg gaggttgttg ttcgtaacgt tgctgctggt tctctttcta agcgtttggg attggaagaa ggtacccctc tttcgcaatc catcattgag ttttattata aaaacgattc tcttgatgat cccatggtaa gcgaagaaca tatcactgct tttggctggg ctgttccaca agaaatagaa gacattatgc agctttcaat tcgtattaat gattttcttt ctggactttt tgcaggagtt aatattcaac tcattgattt taaaatggag tttggccgct tgtgggaaga tgaagcgatg cgtattgttc ttgccgatga aatatcgcct gattctgccc gattatggga tatacaaaca caagaaaaaa tggataaaga tcgttttcgg cgtgatatgg gagggcttat taacgcctat caagaagtgg caaagcgtct tggtattatt aatgaaaatg aaccttcacg accaaacggt ccagttttag tgaagtaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNRRHRIYE GKAKILYEGP EPGTYIQFFK DDATAFNAKK HEIIDGKGVL NNRISEHIFS HLGRLGIPTH FIKRINMREQ LIKAVEIIPL EVVVRNVAAG SLSKRLGLEE GTPLSQSIIE FYYKNDSLDD PMVSEEHITA FGWAVPQEIE DIMQLSIRIN DFLSGLFAGV NIQLIDFKME FGRLWEDEAM RIVLADEISP DSARLWDIQT QEKMDKDRFR RDMGGLINAY QEVAKRLGII NENEPSRPNG PVLVK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATCGTCGC CACCGTATTT ATGAAGGCAA GGCCAAAATT CTGTATGAAG GACCTGAACC AGGTACATAT ATTCAATTTT TTAAAGATGA CGCAACTGCT TTTAATGCAA AAAAGCATGA AATTATCGAT GGAAAAGGAG TTTTAAATAA CCGTATCTCA GAACATATCT TTAGCCATCT TGGTCGTTTA GGCATCCCAA CACACTTTAT CAAGCGTATA AATATGCGCG AACAACTCAT TAAAGCTGTT GAAATTATTC CCTTGGAGGT TGTTGTTCGT AACGTTGCTG CTGGTTCTCT TTCTAAGCGT TTGGGATTGG AAGAAGGTAC CCCTCTTTCG CAATCCATCA TTGAGTTTTA TTATAAAAAC GATTCTCTTG ATGATCCCAT GGTAAGCGAA GAACATATCA CTGCTTTTGG CTGGGCTGTT CCACAAGAAA TAGAAGACAT TATGCAGCTT TCAATTCGTA TTAATGATTT TCTTTCTGGA CTTTTTGCAG GAGTTAATAT TCAACTCATT GATTTTAAAA TGGAGTTTGG CCGCTTGTGG GAAGATGAAG CGATGCGTAT TGTTCTTGCC GATGAAATAT CGCCTGATTC TGCCCGATTA TGGGATATAC AAACACAAGA AAAAATGGAT AAAGATCGTT TTCGGCGTGA TATGGGAGGG CTTATTAACG CCTATCAAGA AGTGGCAAAG CGTCTTGGTA TTATTAATGA AAATGAACCT TCACGACCAA ACGGTCCAGT TTTAGTGAAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA