BaheA.01161.a

pyruvate kinase

CENTER ID: BaheA.01161.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01161.a.A1.GE28393 Full length( BaheA.01161.a ) 1 478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01161.a.A1.PS00701 Full length( BaheA.01161.a ) 1 478

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1741
RefSeq: YP_034210.1
UniProt: Q8L200

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRARKVKII ATLGPSSFSI SMIEKLFMAG ADVFRLNMSH TDRETMVDLV QRIRKVEKTV RRPIGILVDL QGPKLRVGCF DKGQEDLRIG QSFTLDDRDI LGDAQRVFFP HGEVLAAIKP GDRLLIDDGK LELLAQVCDG HSVQCLVVAG NRISDRKGVS LPDTVLPFGP MTLKDKADLQ AVLEQPVDWV ALSFIQRPED IIAVRALTKS KVSLMAKIEK PQALEHIEEI IDVADGIMIA RGDLGVEMPL ERVPAIQMEL IKACRLAGKP VVVATQMLES MITSSVPTRA EVSDVATAVY TGTDAVMLSA ESASGRYPEE AVLMMDRIAQ QIEQDQTYAA QVGAQHPAPE STGTDAISLA ARQIAETLQL TAIVAYTASG TTGVRTSRER PNRPIIALSP IVKTARRLAL VWGLHCVVTE DARDLEDMVD RAAAIAFQEG FCQGGDRFLV TAGVPLGTPG ATNLLRIASV SQDGTKGV
NT Sequence
GTGAAGCGTG CACGTAAAGT AAAAATTATA GCCACTCTTG GCCCTTCTTC GTTTTCTATT TCCATGATTG AGAAGCTTTT TATGGCGGGA GCAGATGTTT TTCGTTTGAA TATGAGCCAT ACTGACCGTG AAACAATGGT TGATTTGGTT CAGCGCATAC GGAAGGTTGA AAAAACTGTT CGCCGGCCAA TCGGTATTTT AGTAGATCTC CAGGGACCAA AATTACGCGT CGGCTGTTTT GATAAAGGGC AAGAAGATTT GCGTATTGGG CAAAGTTTTA CCTTGGATGA TCGTGATATT CTTGGTGATG CGCAGCGTGT TTTTTTTCCG CACGGGGAGG TGCTGGCAGC TATTAAACCC GGTGATCGGT TGTTAATTGA TGATGGAAAG TTGGAACTGC TTGCACAGGT GTGTGATGGT CATTCTGTGC AGTGCCTTGT GGTTGCTGGG AACCGTATTT CTGATCGAAA AGGGGTAAGT CTTCCCGATA CAGTTTTGCC TTTTGGGCCA ATGACTCTCA AGGATAAAGC CGATCTTCAG GCAGTTTTGG AGCAGCCTGT TGATTGGGTT GCTCTGTCTT TTATCCAGCG TCCGGAAGAT ATTATAGCAG TGCGTGCGTT GACCAAAAGC AAAGTGTCTT TGATGGCTAA GATCGAAAAA CCTCAAGCTT TGGAGCATAT AGAAGAGATT ATTGATGTTG CCGATGGTAT TATGATTGCA CGCGGAGATC TGGGGGTAGA AATGCCCTTG GAGAGGGTTC CTGCCATTCA GATGGAACTT ATAAAAGCTT GTCGTTTAGC TGGAAAGCCC GTCGTGGTGG CCACGCAAAT GCTCGAGTCA ATGATTACGT CATCTGTTCC TACAAGGGCA GAAGTTTCTG ATGTTGCGAC AGCAGTTTAT ACGGGAACTG ACGCTGTCAT GTTGTCTGCG GAATCTGCCT CTGGTCGTTA TCCTGAAGAA GCTGTGCTGA TGATGGATCG AATCGCACAA CAAATTGAAC AAGATCAGAC TTATGCGGCT CAAGTTGGCG CACAGCATCC TGCTCCAGAA TCAACGGGGA CCGATGCGAT TTCTCTCGCT GCGCGTCAGA TCGCCGAAAC ACTGCAATTG ACAGCTATCG TTGCTTATAC TGCTTCAGGC ACAACGGGTG TGCGCACATC ACGGGAGCGT CCAAACAGGC CCATTATTGC CTTATCGCCT ATTGTAAAAA CAGCAAGACG TTTGGCTTTG GTCTGGGGAC TTCATTGTGT GGTCACTGAA GACGCGCGGG ATTTGGAGGA TATGGTTGAT CGTGCGGCTG CGATTGCTTT TCAAGAAGGC TTTTGCCAAG GGGGAGATCG CTTCCTTGTG ACAGCTGGTG TTCCTCTTGG TACACCTGGT GCTACCAATC TCTTGCGTAT CGCTTCTGTT TCTCAAGATG GAACAAAGGG TGTT
Details for BaheA.01161.a.A1.GE28393
HARVESTED ON: data unavailable
SEQUENCED ON: 4/16/2012
EXPECTED MW: 54kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 44
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRARKVK IIATLGPSSF SISMIEKLFM AGADVFRLNM SHTDRETMVD LVQRIRKVEK TVRRPIGILV DLQGPKLRVG CFDKGQEDLR IGQSFTLDDR DILGDAQRVF FPHGEVLAAI KPGDRLLIDD GKLELLAQVC DGHSVQCLVV AGNRISDRKG VSLPDTVLPF GPMTLKDKAD LQAVLEQPVD WVALSFIQRP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agcgtgcacg taaagtaaaa attatagcca ctcttggccc ttcttcgttt tctatttcca tgattgagaa gctttttatg gcgggagcag atgtttttcg tttgaatatg agccatactg accgtgaaac aatggttgat ttggttcagc gcatacggaa ggttgaaaaa actgttcgcc ggccaatcgg tattttagta gatctccagg gaccaaaatt acgcgtcggc tgttttgata aagggcaaga agatttgcgt attgggcaaa gttttacctt ggatgatcgt gatattcttg gtgatgcgca gcgtgttttt tttccgcacg gggaggtgct ggcagctatt aaacccggtg atcggttgtt aattgatgat ggaaagttgg aactgcttgc acaggtgtgt gatggtcatt ctgtgcagtg ccttgtggtt gctgggaacc gtatttctga tcgaaaaggg gtaagtcttc ccgatacagt tttgcctttt gggccaatga ctctcaagga taaagccgat cttcaggcag ttttggagca gcctgttgat tgggttgctc tgtcttttat ccagcgtccg taagatatta tagcagttcg tgcgttgacc aaaagcaaag tgtctttgat ggctaagatc gaaaaacctc aagctttgga gcatatagaa gagattattg atgttgccga tggtattatg attgcacgcg gagatctggg ggtagaaatg cccttggaga gggttcctgc cattcagatg gaacttataa aagcttgtcg tttagctgga aagcccgtcg tggtggccac gcaaatgctc gagtcaatga ttacgtcatc tgttcctaca agggcagaag tttctgatgt tgcgacagca gtttatacgg gaactgacgc tgtcatgttg tctgcggaat ctgcctctgg tcgttatcct gaagaagctg tgctgatgat ggatcgaatc gcacaacaaa tcgaacaaga tcagacttat gcggctcaag ttggcgcaca gcatcctgct ccagaatcaa cggggaccga tgcgatttct ctcgctgcgc gtcagatcgc cgaaacactg caattgacag ctatcgttgc ttatactgct tcaggcacaa cgggtgtgcg cacatcacgg gagcgtccaa acaggcccat tattgcctta tcgcctattg taaaaacagc aagacgtttg gctttggtct ggggacttca ttgtgtggtc actgaagacg cgcgggattt ggaggatatg gttgatcgtg cggctgcgat tgcttttcaa gaaggctttt gccaaggggg agatcgcttc cttgtgacag ctggtgttcc tcttggtaca cctggtgcta ccaatctctt gcgtatcgct tctgtttctc aagatggaac aaagggtgtt taat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRARKVK IIATLGPSSF SISMIEKLFM AGADVFRLNM SHTDRETMVD LVQRIRKVEK TVRRPIGILV DLQGPKLRVG CFDKGQEDLR IGQSFTLDDR DILGDAQRVF FPHGEVLAAI KPGDRLLIDD GKLELLAQVC DGHSVQCLVV AGNRISDRKG VSLPDTVLPF GPMTLKDKAD LQAVLEQPVD WVALSFIQRP EDIIAVRALT KSKVSLMAKI EKPQALEHIE EIIDVADGIM IARGDLGVEM PLERVPAIQM ELIKACRLAG KPVVVATQML ESMITSSVPT RAEVSDVATA VYTGTDAVML SAESASGRYP EEAVLMMDRI AQQIEQDQTY AAQVGAQHPA PESTGTDAIS LAARQIAETL QLTAIVAYTA SGTTGVRTSR ERPNRPIIAL SPIVKTARRL ALVWGLHCVV TEDARDLEDM VDRAAAIAFQ EGFCQGGDRF LVTAGVPLGT PGATNLLRIA SVSQDGTKGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagcgt gcacgtaaag taaaaattat agccactctt ggcccttctt cgttttctat ttccatgatt gagaagcttt ttatggcggg agcagatgtt tttcgtttga atatgagcca tactgaccgt gaaacaatgg ttgatttggt tcagcgcata cggaaggttg aaaaaactgt tcgccggcca atcggtattt tagtagatct ccagggacca aaattacgcg tcggctgttt tgataaaggg caagaagatt tgcgtattgg gcaaagtttt accttggatg atcgtgatat tcttggtgat gcgcagcgtg ttttttttcc gcacggggag gtgctggcag ctattaaacc cggtgatcgg ttgttaattg atgatggaaa gttggaactg cttgcacagg tgtgtgatgg tcattctgtg cagtgccttg tggttgctgg gaaccgtatt tctgatcgaa aaggggtaag tcttcccgat acagttttgc cttttgggcc aatgactctc aaggataaag ccgatcttca ggcagttttg gagcagcctg ttgattgggt tgctctgtct tttatccagc gtccggaaga tattatagca gtgcgtgcgt tgaccaaaag caaagtgtct ttgatggcta agatcgaaaa acctcaagct ttggagcata tagaagagat tattgatgtt gccgatggta ttatgattgc acgcggagat ctgggggtag aaatgccctt ggagagggtt cctgccattc agatggaact tataaaagct tgtcgtttag ctggaaagcc cgtcgtggtg gccacgcaaa tgctcgagtc aatgattacg tcatctgttc ctacaagggc agaagtttct gatgttgcga cagcagttta tacgggaact gacgctgtca tgttgtctgc ggaatctgcc tctggtcgtt atcctgaaga agctgtgctg atgatggatc gaatcgcaca acaaattgaa caagatcaga cttatgcggc tcaagttggc gcacagcatc ctgctccaga atcaacgggg accgatgcga tttctctcgc tgcgcgtcag atcgccgaaa cactgcaatt gacagctatc gttgcttata ctgcttcagg cacaacgggt gtgcgcacat cacgggagcg tccaaacagg cccattattg ccttatcgcc tattgtaaaa acagcaagac gtttggcttt ggtctgggga cttcattgtg tggtcactga agacgcgcgg gatttggagg atatggttga tcgtgcggct gcgattgctt ttcaagaagg cttttgccaa gggggagatc gcttccttgt gacagctggt gttcctcttg gtacacctgg tgctaccaat ctcttgcgta tcgcttctgt ttctcaagat ggaacaaagg gtgttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BaheA.01161.a.A1.PS00701
PURIFICATION DATe: 8/2/2010
CONCENTRATION: 14.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 44
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRARKVK IIATLGPSSF SISMIEKLFM AGADVFRLNM SHTDRETMVD LVQRIRKVEK TVRRPIGILV DLQGPKLRVG CFDKGQEDLR IGQSFTLDDR DILGDAQRVF FPHGEVLAAI KPGDRLLIDD GKLELLAQVC DGHSVQCLVV AGNRISDRKG VSLPDTVLPF GPMTLKDKAD LQAVLEQPVD WVALSFIQRP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agcgtgcacg taaagtaaaa attatagcca ctcttggccc ttcttcgttt tctatttcca tgattgagaa gctttttatg gcgggagcag atgtttttcg tttgaatatg agccatactg accgtgaaac aatggttgat ttggttcagc gcatacggaa ggttgaaaaa actgttcgcc ggccaatcgg tattttagta gatctccagg gaccaaaatt acgcgtcggc tgttttgata aagggcaaga agatttgcgt attgggcaaa gttttacctt ggatgatcgt gatattcttg gtgatgcgca gcgtgttttt tttccgcacg gggaggtgct ggcagctatt aaacccggtg atcggttgtt aattgatgat ggaaagttgg aactgcttgc acaggtgtgt gatggtcatt ctgtgcagtg ccttgtggtt gctgggaacc gtatttctga tcgaaaaggg gtaagtcttc ccgatacagt tttgcctttt gggccaatga ctctcaagga taaagccgat cttcaggcag ttttggagca gcctgttgat tgggttgctc tgtcttttat ccagcgtccg taagatatta tagcagttcg tgcgttgacc aaaagcaaag tgtctttgat ggctaagatc gaaaaacctc aagctttgga gcatatagaa gagattattg atgttgccga tggtattatg attgcacgcg gagatctggg ggtagaaatg cccttggaga gggttcctgc cattcagatg gaacttataa aagcttgtcg tttagctgga aagcccgtcg tggtggccac gcaaatgctc gagtcaatga ttacgtcatc tgttcctaca agggcagaag tttctgatgt tgcgacagca gtttatacgg gaactgacgc tgtcatgttg tctgcggaat ctgcctctgg tcgttatcct gaagaagctg tgctgatgat ggatcgaatc gcacaacaaa tcgaacaaga tcagacttat gcggctcaag ttggcgcaca gcatcctgct ccagaatcaa cggggaccga tgcgatttct ctcgctgcgc gtcagatcgc cgaaacactg caattgacag ctatcgttgc ttatactgct tcaggcacaa cgggtgtgcg cacatcacgg gagcgtccaa acaggcccat tattgcctta tcgcctattg taaaaacagc aagacgtttg gctttggtct ggggacttca ttgtgtggtc actgaagacg cgcgggattt ggaggatatg gttgatcgtg cggctgcgat tgcttttcaa gaaggctttt gccaaggggg agatcgcttc cttgtgacag ctggtgttcc tcttggtaca cctggtgcta ccaatctctt gcgtatcgct tctgtttctc aagatggaac aaagggtgtt taat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRARKVK IIATLGPSSF SISMIEKLFM AGADVFRLNM SHTDRETMVD LVQRIRKVEK TVRRPIGILV DLQGPKLRVG CFDKGQEDLR IGQSFTLDDR DILGDAQRVF FPHGEVLAAI KPGDRLLIDD GKLELLAQVC DGHSVQCLVV AGNRISDRKG VSLPDTVLPF GPMTLKDKAD LQAVLEQPVD WVALSFIQRP EDIIAVRALT KSKVSLMAKI EKPQALEHIE EIIDVADGIM IARGDLGVEM PLERVPAIQM ELIKACRLAG KPVVVATQML ESMITSSVPT RAEVSDVATA VYTGTDAVML SAESASGRYP EEAVLMMDRI AQQIEQDQTY AAQVGAQHPA PESTGTDAIS LAARQIAETL QLTAIVAYTA SGTTGVRTSR ERPNRPIIAL SPIVKTARRL ALVWGLHCVV TEDARDLEDM VDRAAAIAFQ EGFCQGGDRF LVTAGVPLGT PGATNLLRIA SVSQDGTKGV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGCGT GCACGTAAAG TAAAAATTAT AGCCACTCTT GGCCCTTCTT CGTTTTCTAT TTCCATGATT GAGAAGCTTT TTATGGCGGG AGCAGATGTT TTTCGTTTGA ATATGAGCCA TACTGACCGT GAAACAATGG TTGATTTGGT TCAGCGCATA CGGAAGGTTG AAAAAACTGT TCGCCGGCCA ATCGGTATTT TAGTAGATCT CCAGGGACCA AAATTACGCG TCGGCTGTTT TGATAAAGGG CAAGAAGATT TGCGTATTGG GCAAAGTTTT ACCTTGGATG ATCGTGATAT TCTTGGTGAT GCGCAGCGTG TTTTTTTTCC GCACGGGGAG GTGCTGGCAG CTATTAAACC CGGTGATCGG TTGTTAATTG ATGATGGAAA GTTGGAACTG CTTGCACAGG TGTGTGATGG TCATTCTGTG CAGTGCCTTG TGGTTGCTGG GAACCGTATT TCTGATCGAA AAGGGGTAAG TCTTCCCGAT ACAGTTTTGC CTTTTGGGCC AATGACTCTC AAGGATAAAG CCGATCTTCA GGCAGTTTTG GAGCAGCCTG TTGATTGGGT TGCTCTGTCT TTTATCCAGC GTCCGGAAGA TATTATAGCA GTGCGTGCGT TGACCAAAAG CAAAGTGTCT TTGATGGCTA AGATCGAAAA ACCTCAAGCT TTGGAGCATA TAGAAGAGAT TATTGATGTT GCCGATGGTA TTATGATTGC ACGCGGAGAT CTGGGGGTAG AAATGCCCTT GGAGAGGGTT CCTGCCATTC AGATGGAACT TATAAAAGCT TGTCGTTTAG CTGGAAAGCC CGTCGTGGTG GCCACGCAAA TGCTCGAGTC AATGATTACG TCATCTGTTC CTACAAGGGC AGAAGTTTCT GATGTTGCGA CAGCAGTTTA TACGGGAACT GACGCTGTCA TGTTGTCTGC GGAATCTGCC TCTGGTCGTT ATCCTGAAGA AGCTGTGCTG ATGATGGATC GAATCGCACA ACAAATTGAA CAAGATCAGA CTTATGCGGC TCAAGTTGGC GCACAGCATC CTGCTCCAGA ATCAACGGGG ACCGATGCGA TTTCTCTCGC TGCGCGTCAG ATCGCCGAAA CACTGCAATT GACAGCTATC GTTGCTTATA CTGCTTCAGG CACAACGGGT GTGCGCACAT CACGGGAGCG TCCAAACAGG CCCATTATTG CCTTATCGCC TATTGTAAAA ACAGCAAGAC GTTTGGCTTT GGTCTGGGGA CTTCATTGTG TGGTCACTGA AGACGCGCGG GATTTGGAGG ATATGGTTGA TCGTGCGGCT GCGATTGCTT TTCAAGAAGG CTTTTGCCAA GGGGGAGATC GCTTCCTTGT GACAGCTGGT GTTCCTCTTG GTACACCTGG TGCTACCAAT CTCTTGCGTA TCGCTTCTGT TTCTCAAGAT GGAACAAAGG GTGTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA