SSGCID: Target Page

BaheA.01135.a

succinyl-CoA synthetase subunit alpha

CENTER ID:	BaheA.01135.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01135.a.A1.GE30631	Full length( BaheA.01135.a )		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1925
RefSeq:	YP_034345.1
UniProt:	A0A0H3LYT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSILVNKDTK VLVQGLTGKT GTFHTEQALA YHGTQMVGGV NPKKGGETWE GSKGETLPIF ASVAEGKEKT GADASVIYVP PAGAAAAIIE AVEAEIRLII CITEGIPVMD MVKVKARLEK SKSRLIGPNC PGILTPNECK IGIMPGSIFR KGSVGVVSRS GTLTYEAVFQ TSHEGLGQTT AIGIGGDPVK GTEFIDVLEM FLADDETQSI VMIGEIGGSA EEEAAQFLQD EAKKGRKKPV VGFIAGRTAP PGRTMGHAGA VISGGKGGAE DKIAAMESAG IKVSPSPSQI GKTLMSVLKG

NT Sequence

ATGTCAATTC TTGTGAATAA AGATACGAAA GTTCTCGTTC AAGGGCTTAC AGGAAAAACA GGAACTTTTC ATACAGAACA AGCGCTTGCT TATCATGGTA CCCAAATGGT TGGCGGCGTT AATCCTAAAA AGGGTGGTGA GACATGGGAG GGTTCAAAAG GTGAAACTCT TCCTATTTTT GCTAGTGTTG CGGAAGGAAA AGAAAAAACA GGTGCAGATG CTTCCGTTAT TTATGTTCCT CCTGCAGGGG CAGCAGCAGC TATTATAGAG GCTGTTGAAG CTGAAATCCG TTTAATTATC TGTATTACGG AAGGCATTCC TGTTATGGAT ATGGTGAAAG TAAAAGCGAG ATTAGAAAAA TCAAAATCAC GTCTCATTGG TCCTAATTGT CCTGGCATTC TCACACCAAA TGAATGTAAA ATCGGTATTA TGCCTGGTTC TATTTTTAGA AAAGGCTCTG TTGGGGTTGT CTCACGGTCG GGAACTTTGA CTTATGAAGC CGTCTTTCAA ACCAGTCATG AAGGTCTTGG ACAGACAACA GCTATTGGTA TTGGCGGCGA TCCTGTTAAG GGCACTGAAT TTATTGATGT GTTAGAAATG TTTTTAGCAG ATGATGAAAC TCAATCTATT GTTATGATTG GTGAGATTGG TGGTTCTGCC GAAGAAGAAG CAGCACAGTT TCTCCAAGAT GAGGCAAAAA AAGGCCGTAA AAAGCCGGTG GTTGGTTTTA TTGCTGGTCG TACAGCTCCT CCAGGACGCA CAATGGGGCA TGCGGGTGCT GTTATTTCTG GTGGAAAAGG TGGTGCAGAA GATAAAATCG CAGCAATGGA ATCAGCAGGA ATTAAAGTTT CTCCTTCACC CTCACAGATA GGAAAGACAT TGATGTCAGT TTTGAAGGGA

Details for BaheA.01135.a.A1.GE30631

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	33kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	59

Validated AA Sequence

MGTLEAQTQG PGSMSILVNK DTKVLVQGLT GKTGTFHTEQ ALAYHGTQMV GGVNPKKGGE TWEGSKGETL PIFASVAEGK EKTGADASVI YVPPAGAAAA IIEAVEAEIR LIICITEGIP VMDMVKVKAR LEKSKSRLIG PNCPGILTPN ECKIGIMPGS IFRKGSVGVV SRXGTLTYEA VFQTXHEXLG Q

Validated NT Sequence

ggctcntcac cntcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcaatt cttgtgaata aagatacgaa agttctcgtt caagggctta caggaaaaac aggaactttt catacagaac aagcgcttgc ttatcatggt acccaaatgg ttggcggcgt taatcctaaa aagggtggtg agacatggga gggttcaaaa ggtgaaactc ttcctatttt tgctagtgtt gcggaaggaa aagaaaaaac aggtgcagat gcttccgtta tttatgttcc tcctgcaggg gcagcagcag ctattataga ggctgttgaa gctgaaatcc gtttaattat ctgtattacg gaaggcattc ctgttatgga tatggtgaaa gtaaaagcga gattagaaaa atcaaaatca cgtctcattg gtcctaattg tcctggcatt ctcacaccaa atgaatgtaa aatcggtatt atgcctggtt ctatttttag aaaaggctct gttggggttg tctcacggnc gggaactttg acttatgaag ccgtctttca aaccantcat gaangtcttg gacagac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSILVNKDT KVLVQGLTGK TGTFHTEQAL AYHGTQMVGG VNPKKGGETW EGSKGETLPI FASVAEGKEK TGADASVIYV PPAGAAAAII EAVEAEIRLI ICITEGIPVM DMVKVKARLE KSKSRLIGPN CPGILTPNEC KIGIMPGSIF RKGSVGVVSR SGTLTYEAVF QTSHEGLGQT TAIGIGGDPV KGTEFIDVLE MFLADDETQS IVMIGEIGGS AEEEAAQFLQ DEAKKGRKKP VVGFIAGRTA PPGRTMGHAG AVISGGKGGA EDKIAAMESA GIKVSPSPSQ IGKTLMSVLK G

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaattctt gtgaataaag atacgaaagt tctcgttcaa gggcttacag gaaaaacagg aacttttcat acagaacaag cgcttgctta tcatggtacc caaatggttg gcggcgttaa tcctaaaaag ggtggtgaga catgggaggg ttcaaaaggt gaaactcttc ctatttttgc tagtgttgcg gaaggaaaag aaaaaacagg tgcagatgct tccgttattt atgttcctcc tgcaggggca gcagcagcta ttatagaggc tgttgaagct gaaatccgtt taattatctg tattacggaa ggcattcctg ttatggatat ggtgaaagta aaagcgagat tagaaaaatc aaaatcacgt ctcattggtc ctaattgtcc tggcattctc acaccaaatg aatgtaaaat cggtattatg cctggttcta tttttagaaa aggctctgtt ggggttgtct cacggtcggg aactttgact tatgaagccg tctttcaaac cagtcatgaa ggtcttggac agacaacagc tattggtatt ggcggcgatc ctgttaaggg cactgaattt attgatgtgt tagaaatgtt tttagcagat gatgaaactc aatctattgt tatgattggt gagattggtg gttctgccga agaagaagca gcacagtttc tccaagatga ggcaaaaaaa ggccgtaaaa agccggtggt tggttttatt gctggtcgta cagctcctcc aggacgcaca atggggcatg cgggtgctgt tatttctggt ggaaaaggtg gtgcagaaga taaaatcgca gcaatggaat cagcaggaat taaagtttct ccttcaccct cacagatagg aaagacattg atgtcagttt tgaagggaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa