BaheA.01128.a

fumarate hydratase

CENTER ID: BaheA.01128.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01128.a.A1.GE26085 Full length( BaheA.01128.a ) 1 462
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01128.a.A1.PS00190 Full length( BaheA.01128.a ) 1 462

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1483
RefSeq: YP_034077.1
UniProt: A0A0H3LXR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVETRQETDS FGTISVRQDR YWGAQTERSL HNFNIGIEKQ PLTIIYALSL IKKAAAVVNM EKGKLAKNIG KAIITAADEV LSGAFDTHFP LSVWQTGSGT QSNMNVNEVI ANHANMLLGG KLGSKTPVHP NDHVNMSQSS NDSFPTALHI ATTLQTRQHL FPILDALITT LKKKEEEFAD IIKIGRTHTQ DATPLTLAQE FSGYRTALEA NRQRIENALC DVQMLAQGGT AVGTGLNAPQ GFDIAFAQTI STFTGIPFKT ASNKFEALAH HGALAHFHGS LNALAADLFK IANDIRFLGS GPRSGLGELS LPENEPGSSI MPGKVNPTQC EAMTMVACQV FGNHTSVTFA ASQGHFELNV YKPVIGYNVL QSITLLGDCM RSFDLHCIQG LRANRVHIHS LMERSLMLVT ALAPEIGYEK AAAIAKAAHK NDTTLRTEAL KLGVSGEDYD RLVDPKKMIH PQ
NT Sequence
ATGGTTGAAA CACGTCAAGA AACGGATAGC TTTGGGACGA TTTCAGTCCG TCAAGACCGT TATTGGGGCG CACAAACTGA ACGCTCATTG CATAATTTTA ATATTGGCAT AGAAAAACAA CCCCTCACCA TCATCTATGC CTTAAGCCTT ATCAAAAAAG CAGCAGCTGT TGTGAATATG GAAAAAGGCA AACTCGCAAA AAACATTGGA AAAGCCATCA TTACTGCTGC CGATGAAGTG CTTTCCGGTG CATTTGACAC GCATTTCCCC CTTTCTGTTT GGCAAACAGG TTCTGGAACA CAAAGCAATA TGAACGTCAA TGAAGTGATC GCCAATCATG CCAATATGCT TTTGGGAGGA AAATTGGGTA GCAAAACACC TGTGCATCCC AATGATCACG TCAATATGAG CCAATCGTCA AACGATTCCT TTCCCACAGC ACTTCACATT GCTACTACCC TACAAACGCG CCAACACCTC TTTCCTATCC TTGACGCACT TATTACCACT TTAAAGAAAA AAGAAGAAGA ATTTGCCGAT ATCATCAAAA TTGGACGTAC TCATACCCAG GATGCCACAC CCTTAACTTT AGCGCAAGAA TTTTCGGGCT ATCGCACTGC ACTCGAAGCC AACCGCCAAC GCATTGAAAA TGCTCTTTGT GATGTTCAAA TGCTTGCCCA AGGAGGTACA GCTGTTGGAA CAGGGCTGAA CGCACCGCAA GGCTTTGACA TAGCTTTTGC GCAAACAATC AGTACATTTA CAGGAATACC CTTTAAAACC GCTAGCAATA AATTTGAAGC CCTCGCTCAT CATGGAGCTC TTGCGCATTT CCATGGTAGC TTAAATGCAT TAGCAGCTGA TCTGTTTAAA ATTGCCAATG ACATTCGGTT TTTGGGATCC GGTCCACGCT CAGGCTTGGG GGAACTAAGC TTACCAGAAA ATGAACCAGG ATCTTCCATC ATGCCAGGCA AGGTTAACCC CACACAGTGT GAAGCAATGA CCATGGTTGC TTGCCAAGTC TTTGGCAATC ATACCAGTGT AACATTTGCC GCAAGCCAAG GGCATTTTGA ACTCAACGTT TATAAACCTG TCATTGGCTA CAATGTTTTA CAATCGATTA CGCTTCTTGG GGATTGCATG CGCTCTTTTG ATCTTCATTG TATTCAAGGA TTACGCGCCA ATCGAGTTCA CATTCATTCT CTCATGGAAC GTTCTCTCAT GTTGGTGACA GCGCTTGCAC CAGAAATTGG CTATGAAAAA GCTGCCGCAA TTGCCAAAGC AGCGCATAAA AATGACACAA CTTTGCGTAC CGAAGCACTC AAACTAGGAG TTTCTGGAGA AGATTATGAT CGGTTGGTTG ATCCCAAAAA GATGATCCAT CCACAA
Details for BaheA.01128.a.A1.GE26085
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 52kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 58
Validated AA Sequence
MGTLEAQTQG PGSMVETRQE TDSFGTISVR QDRYWGAQTE RSLHNFNIGI EKQPLTIIYA LSLIKKAAAV VNMEKGKLAK NIGKAIITAA DEVLSGAFDT HFPLSVWQTG SGTQSNMNVN EVIANHANML LGGKLGSKTP VHPNDHVNMS QSSNDSFPTA LHIATTLQTR QHLFPILDAL ITTLKKKEEE FADIIKIGRT HTQDATPLTL AQEFSGYRTA LEANRQRIEX SLCDVQMLAQ GXTAXXTGLN APQGFDIXFA XTISTFTGIP FKTASNKFEA LAHH
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggttga aacacgtcaa gaaacggata gctttgggac gatttcagtc cgtcaagacc gttattgggg cgcacaaact gaacgctcat tgcataattt taatattggc atagaaaaac aacccctcac catcatctat gccttaagcc ttatcaaaaa agcagcagct gttgtgaata tggaaaaagg caaactcgca aaaaacattg gaaaagccat cattactgct gccgatgaag tgctttccgg tgcatttgac acgcatttcc ccctttctgt ttggcaaaca ggttctggaa cacaaagcaa tatgaacgtc aatgaagtga tcgccaatca tgccaatatg cttttgggag gaaaattggg tagcaaaaca cctgtgcatc ccaatgatca cgtcaatatg agccaatcgt caaacgattc ctttcccaca gcacttcaca ttgctactac cctacaaacg cgccaacacc tctttcctat ccttgacgca cttattacca ctttaaagaa aaaagaagaa gaatttgccg atatcatcaa aattggacgt actcataccc aggatgccac acccttaact ttagcgcaag aattttcggg ctatcgcact gcactcgaag ccaaccgcca acgcattgaa aantctcttt gtgatgttca aatgcttgcc caaggangta cagctgntgn aacagggctg aacgcaccgc aaggctttga catanctttt gcgcanacaa tcagtacatt tacaggaata ccctttaaaa ccgctagcaa taaatttgaa gccctcgctc atcatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVETRQETD SFGTISVRQD RYWGAQTERS LHNFNIGIEK QPLTIIYALS LIKKAAAVVN MEKGKLAKNI GKAIITAADE VLSGAFDTHF PLSVWQTGSG TQSNMNVNEV IANHANMLLG GKLGSKTPVH PNDHVNMSQS SNDSFPTALH IATTLQTRQH LFPILDALIT TLKKKEEEFA DIIKIGRTHT QDATPLTLAQ EFSGYRTALE ANRQRIENAL CDVQMLAQGG TAVGTGLNAP QGFDIAFAQT ISTFTGIPFK TASNKFEALA HHGALAHFHG SLNALAADLF KIANDIRFLG SGPRSGLGEL SLPENEPGSS IMPGKVNPTQ CEAMTMVACQ VFGNHTSVTF AASQGHFELN VYKPVIGYNV LQSITLLGDC MRSFDLHCIQ GLRANRVHIH SLMERSLMLV TALAPEIGYE KAAAIAKAAH KNDTTLRTEA LKLGVSGEDY DRLVDPKKMI HPQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgaaaca cgtcaagaaa cggatagctt tgggacgatt tcagtccgtc aagaccgtta ttggggcgca caaactgaac gctcattgca taattttaat attggcatag aaaaacaacc cctcaccatc atctatgcct taagccttat caaaaaagca gcagctgttg tgaatatgga aaaaggcaaa ctcgcaaaaa acattggaaa agccatcatt actgctgccg atgaagtgct ttccggtgca tttgacacgc atttccccct ttctgtttgg caaacaggtt ctggaacaca aagcaatatg aacgtcaatg aagtgatcgc caatcatgcc aatatgcttt tgggaggaaa attgggtagc aaaacacctg tgcatcccaa tgatcacgtc aatatgagcc aatcgtcaaa cgattccttt cccacagcac ttcacattgc tactacccta caaacgcgcc aacacctctt tcctatcctt gacgcactta ttaccacttt aaagaaaaaa gaagaagaat ttgccgatat catcaaaatt ggacgtactc atacccagga tgccacaccc ttaactttag cgcaagaatt ttcgggctat cgcactgcac tcgaagccaa ccgccaacgc attgaaaatg ctctttgtga tgttcaaatg cttgcccaag gaggtacagc tgttggaaca gggctgaacg caccgcaagg ctttgacata gcttttgcgc aaacaatcag tacatttaca ggaataccct ttaaaaccgc tagcaataaa tttgaagccc tcgctcatca tggagctctt gcgcatttcc atggtagctt aaatgcatta gcagctgatc tgtttaaaat tgccaatgac attcggtttt tgggatccgg tccacgctca ggcttggggg aactaagctt accagaaaat gaaccaggat cttccatcat gccaggcaag gttaacccca cacagtgtga agcaatgacc atggttgctt gccaagtctt tggcaatcat accagtgtaa catttgccgc aagccaaggg cattttgaac tcaacgttta taaacctgtc attggctaca atgttttaca atcgattacg cttcttgggg attgcatgcg ctcttttgat cttcattgta ttcaaggatt acgcgccaat cgagttcaca ttcattctct catggaacgt tctctcatgt tggtgacagc gcttgcacca gaaattggct atgaaaaagc tgccgcaatt gccaaagcag cgcataaaaa tgacacaact ttgcgtaccg aagcactcaa actaggagtt tctggagaag attatgatcg gttggttgat cccaaaaaga tgatccatcc acaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.01128.a.A1.PS00190
PURIFICATION DATe: 3/18/2009
CONCENTRATION: 33.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 58
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVETRQE TDSFGTISVR QDRYWGAQTE RSLHNFNIGI EKQPLTIIYA LSLIKKAAAV VNMEKGKLAK NIGKAIITAA DEVLSGAFDT HFPLSVWQTG SGTQSNMNVN EVIANHANML LGGKLGSKTP VHPNDHVNMS QSSNDSFPTA LHIATTLQTR QHLFPILDAL ITTLKKKEEE FADIIKIGRT HTQDATPLTL AQEFSGYRTA LEANRQRIEX SLCDVQMLAQ GXTAXXTGLN APQGFDIXFA XTISTFTGIP FKTASNKFEA LAHH
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggttga aacacgtcaa gaaacggata gctttgggac gatttcagtc cgtcaagacc gttattgggg cgcacaaact gaacgctcat tgcataattt taatattggc atagaaaaac aacccctcac catcatctat gccttaagcc ttatcaaaaa agcagcagct gttgtgaata tggaaaaagg caaactcgca aaaaacattg gaaaagccat cattactgct gccgatgaag tgctttccgg tgcatttgac acgcatttcc ccctttctgt ttggcaaaca ggttctggaa cacaaagcaa tatgaacgtc aatgaagtga tcgccaatca tgccaatatg cttttgggag gaaaattggg tagcaaaaca cctgtgcatc ccaatgatca cgtcaatatg agccaatcgt caaacgattc ctttcccaca gcacttcaca ttgctactac cctacaaacg cgccaacacc tctttcctat ccttgacgca cttattacca ctttaaagaa aaaagaagaa gaatttgccg atatcatcaa aattggacgt actcataccc aggatgccac acccttaact ttagcgcaag aattttcggg ctatcgcact gcactcgaag ccaaccgcca acgcattgaa aantctcttt gtgatgttca aatgcttgcc caaggangta cagctgntgn aacagggctg aacgcaccgc aaggctttga catanctttt gcgcanacaa tcagtacatt tacaggaata ccctttaaaa ccgctagcaa taaatttgaa gccctcgctc atcatg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVETRQETD SFGTISVRQD RYWGAQTERS LHNFNIGIEK QPLTIIYALS LIKKAAAVVN MEKGKLAKNI GKAIITAADE VLSGAFDTHF PLSVWQTGSG TQSNMNVNEV IANHANMLLG GKLGSKTPVH PNDHVNMSQS SNDSFPTALH IATTLQTRQH LFPILDALIT TLKKKEEEFA DIIKIGRTHT QDATPLTLAQ EFSGYRTALE ANRQRIENAL CDVQMLAQGG TAVGTGLNAP QGFDIAFAQT ISTFTGIPFK TASNKFEALA HHGALAHFHG SLNALAADLF KIANDIRFLG SGPRSGLGEL SLPENEPGSS IMPGKVNPTQ CEAMTMVACQ VFGNHTSVTF AASQGHFELN VYKPVIGYNV LQSITLLGDC MRSFDLHCIQ GLRANRVHIH SLMERSLMLV TALAPEIGYE KAAAIAKAAH KNDTTLRTEA LKLGVSGEDY DRLVDPKKMI HPQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGAAACA CGTCAAGAAA CGGATAGCTT TGGGACGATT TCAGTCCGTC AAGACCGTTA TTGGGGCGCA CAAACTGAAC GCTCATTGCA TAATTTTAAT ATTGGCATAG AAAAACAACC CCTCACCATC ATCTATGCCT TAAGCCTTAT CAAAAAAGCA GCAGCTGTTG TGAATATGGA AAAAGGCAAA CTCGCAAAAA ACATTGGAAA AGCCATCATT ACTGCTGCCG ATGAAGTGCT TTCCGGTGCA TTTGACACGC ATTTCCCCCT TTCTGTTTGG CAAACAGGTT CTGGAACACA AAGCAATATG AACGTCAATG AAGTGATCGC CAATCATGCC AATATGCTTT TGGGAGGAAA ATTGGGTAGC AAAACACCTG TGCATCCCAA TGATCACGTC AATATGAGCC AATCGTCAAA CGATTCCTTT CCCACAGCAC TTCACATTGC TACTACCCTA CAAACGCGCC AACACCTCTT TCCTATCCTT GACGCACTTA TTACCACTTT AAAGAAAAAA GAAGAAGAAT TTGCCGATAT CATCAAAATT GGACGTACTC ATACCCAGGA TGCCACACCC TTAACTTTAG CGCAAGAATT TTCGGGCTAT CGCACTGCAC TCGAAGCCAA CCGCCAACGC ATTGAAAATG CTCTTTGTGA TGTTCAAATG CTTGCCCAAG GAGGTACAGC TGTTGGAACA GGGCTGAACG CACCGCAAGG CTTTGACATA GCTTTTGCGC AAACAATCAG TACATTTACA GGAATACCCT TTAAAACCGC TAGCAATAAA TTTGAAGCCC TCGCTCATCA TGGAGCTCTT GCGCATTTCC ATGGTAGCTT AAATGCATTA GCAGCTGATC TGTTTAAAAT TGCCAATGAC ATTCGGTTTT TGGGATCCGG TCCACGCTCA GGCTTGGGGG AACTAAGCTT ACCAGAAAAT GAACCAGGAT CTTCCATCAT GCCAGGCAAG GTTAACCCCA CACAGTGTGA AGCAATGACC ATGGTTGCTT GCCAAGTCTT TGGCAATCAT ACCAGTGTAA CATTTGCCGC AAGCCAAGGG CATTTTGAAC TCAACGTTTA TAAACCTGTC ATTGGCTACA ATGTTTTACA ATCGATTACG CTTCTTGGGG ATTGCATGCG CTCTTTTGAT CTTCATTGTA TTCAAGGATT ACGCGCCAAT CGAGTTCACA TTCATTCTCT CATGGAACGT TCTCTCATGT TGGTGACAGC GCTTGCACCA GAAATTGGCT ATGAAAAAGC TGCCGCAATT GCCAAAGCAG CGCATAAAAA TGACACAACT TTGCGTACCG AAGCACTCAA ACTAGGAGTT TCTGGAGAAG ATTATGATCG GTTGGTTGAT CCCAAAAAGA TGATCCATCC ACAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA