SSGCID: Target Page

BaheA.01110.a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE/YchB)

CENTER ID:	BaheA.01110.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01110.a.A1.GE34611	Full length( BaheA.01110.a )		1	302

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.458
RefSeq:	YP_033260.1
UniProt:	Q6G4E4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MISNGQAFFQ APSYLFTPIK LNLALHVVGQ RADGYHLIES LVYFSLSGDC LSCTPFGSNR FVLTGPFADE LVSDAENLVV RAHDFMCNTF PECAKPAFFQ LVKLLPVASG VGGGSGNAAG VLSLLRQQWA LDCSCEKLAE MSLVLGADVP MCLFALEYQQ PLFVKGIGQD ITRLKEACSL AMVLVNHGQQ IATKTVFKAL EKRDHPPLKI DSAALKTVDS LVEALQETRN DLFVPALKIA PQLSEVLCAL DESGALFSRM SGTGATCFGI FKDKQAAQQA ALFIKAMYPD WFVKPIVTLA KL

NT Sequence

ATGATATCGA ATGGACAAGC TTTTTTTCAA GCGCCTTCTT ATTTATTTAC CCCTATTAAA TTGAACTTAG CTTTGCATGT TGTAGGGCAA CGCGCTGATG GTTATCATTT AATAGAAAGT TTGGTTTATT TTAGTCTCAG TGGTGATTGT TTAAGCTGCA CACCTTTTGG GAGTAACCGC TTTGTTTTAA CAGGTCCTTT TGCAGATGAG CTTGTTTCTG ATGCAGAAAA TTTGGTTGTT CGTGCACATG ATTTTATGTG TAACACCTTT CCTGAATGTG CTAAACCTGC TTTTTTTCAA CTTGTTAAAT TATTGCCGGT TGCTTCAGGG GTTGGTGGTG GTTCGGGTAA TGCTGCTGGT GTTTTGAGTC TGTTGCGTCA GCAATGGGCT CTTGATTGCT CTTGTGAAAA ATTAGCGGAA ATGAGTTTAG TTCTTGGCGC TGATGTACCA ATGTGTCTTT TTGCATTGGA GTATCAGCAG CCACTTTTTG TTAAAGGAAT TGGGCAAGAT ATAACACGGC TAAAAGAGGC TTGTTCTCTT GCAATGGTTT TAGTTAATCA TGGTCAACAG ATTGCGACAA AAACTGTTTT TAAGGCTTTA GAGAAGCGCG ATCATCCACC CTTGAAAATT GATTCGGCAG CTCTAAAGAC GGTTGATTCA TTGGTTGAAG CTTTACAGGA AACGCGTAAC GATCTTTTTG TTCCTGCCTT AAAAATTGCA CCTCAACTGT CTGAGGTTTT ATGTGCATTA GATGAGAGTG GAGCTCTTTT TTCTCGCATG TCCGGGACTG GTGCAACTTG TTTTGGTATT TTTAAAGATA AGCAAGCAGC GCAACAGGCT GCTCTTTTTA TTAAAGCGAT GTATCCAGAC TGGTTTGTTA AACCTATTGT AACTTTAGCA AAGCTC

Details for BaheA.01110.a.A1.GE34611

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	92

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMISNGQAFF QAPSYLFTPI KLNLALHVVG QRADGYHLIE SLVYFSLSGD CLSCTPFGSN RFVLTGPFAD ELVSDAENLV VRAHDFMCNT FPECAKPAFF QLVKLLPVAS GVGGGSGNAA GVLSLLRQQW ALDCSCEKLA EMSLVLGADV PMCLFALEYQ QPLFVKGIGQ DITRLKEACS LAMVLVNHGQ QIATKTVFKA LEKRDHPPLK IDSAALKTVD SLVEALQETR NDLFVPALKI APQLSXVLCA LDESGALFSR MSXTGATCFG IFKXXQAAQ

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatat cgaatggaca agcttttttt caagcgcctt cttatttatt tacccctatt aaattgaact tagctttgca tgttgtaggg caacgcgctg atggttatca tttaatagaa agtttggttt attttagtct cagtggtgat tgtttaagct gcacaccttt tgggagtaac cgctttgttt taacaggtcc ttttgcagat gagcttgttt ctgatgcaga aaatttggtt gttcgtgcac atgattttat gtgtaacacc tttcctgaat gtgctaaacc tgcttttttt caacttgtta aattattgcc ggttgcttca ggggttggtg gtggttcggg taatgctgct ggtgttttga gtctgttgcg tcagcaatgg gctcttgatt gctcttgtga aaaattagcg gaaatgagtt tagttcttgg cgctgatgta ccaatgtgtc tttttgcatt ggagtatcag cagccacttt ttgttaaagg aattgggcaa gatataacac ggctaaaaga ggcttgttct cttgcaatgg ttttagttaa tcatggtcaa cagattgcga caaaaactgt ttttaaggct ttagagaagc gcgatcatcc acccttgaaa attgattcgg cagctctaaa gacggttgat tcattggttg aagctttaca ggaaacgcgt aacgatcttt ttgttcctgc cttaaaaatt gcacctcaac tgtctgangt tttatgtgca ttagatgaga gtggagctct tttttctcgc atgtccngga ctggtgcaac ttgttttggt atttttaaan anaancaagc agcgcaac

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatatcgaat ggacaagctt tttttcaagc gccttcttat ttatttaccc ctattaaatt gaacttagct ttgcatgttg tagggcaacg cgctgatggt tatcatttaa tagaaagttt ggtttatttt agtctcagtg gtgattgttt aagctgcaca ccttttggga gtaaccgctt tgttttaaca ggtccttttg cagatgagct tgtttctgat gcagaaaatt tggttgttcg tgcacatgat tttatgtgta acacctttcc tgaatgtgct aaacctgctt tttttcaact tgttaaatta ttgccggttg cttcaggggt tggtggtggt tcgggtaatg ctgctggtgt tttgagtctg ttgcgtcagc aatgggctct tgattgctct tgtgaaaaat tagcggaaat gagtttagtt cttggcgctg atgtaccaat gtgtcttttt gcattggagt atcagcagcc actttttgtt aaaggaattg ggcaagatat aacacggcta aaagaggctt gttctcttgc aatggtttta gttaatcatg gtcaacagat tgcgacaaaa actgttttta aggctttaga gaagcgcgat catccaccct tgaaaattga ttcggcagct ctaaagacgg ttgattcatt ggttgaagct ttacaggaaa cgcgtaacga tctttttgtt cctgccttaa aaattgcacc tcaactgtct gaggttttat gtgcattaga tgagagtgga gctctttttt ctcgcatgtc cgggactggt gcaacttgtt ttggtatttt taaagataag caagcagcgc aacaggctgc tctttttatt aaagcgatgt atccagactg gtttgttaaa cctattgtaa ctttagcaaa gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa