SSGCID: Target Page

BaheA.01104.a

Nitrogenase cofactor synthesis protein nifS

CENTER ID:	BaheA.01104.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01104.a.A1.GE26077	Full length( BaheA.01104.a )		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.01104.a.A1.PS00209	Full length( BaheA.01104.a )		1	384

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.914
RefSeq:	YP_033669.1
UniProt:	A0A0H3M3E9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVVKRRYFDH NATTPLKKAA EVALLESLEI FGNPSSVHTE GRAAKALLQK ARRQIAKSLN ANPDHVVFTS GASEAAMTLL TPFYNMGSSK VQFSHLYIGA TEHPSVAEGG RFPKEFISTI AVDQDGLIQQ DKLLSLLSFH DKTKGLPLVA IQAANGETGV MQQIKEIAAI VRNCGGTLIV DLTQYIDKNF VDVHQMEGDF FILSAHKIGG PKGIGAFVSC GNLLMPYPLI VGGGQEKGLR GGTEALPLIA AFGAAMADCY NQEEIKQLLY LRHKLEDGIQ KICHDVEIFG KKVQRLPNTT YFTVRNIKAE TLQISFDLAG FSVSAGSACS SGKVKQSRVL EAMGYDVPNG AIRISTGRCT TSKDIDDFLL FFSQIINKKK AGTL

NT Sequence

ATGGTTGTAA AACGTCGATA TTTTGATCAT AACGCGACAA CACCGCTTAA GAAGGCGGCA GAGGTAGCGT TACTGGAATC TTTAGAGATC TTTGGTAATC CCTCATCCGT TCATACGGAA GGACGTGCTG CTAAAGCTTT ATTACAAAAG GCACGTCGAC AAATTGCTAA AAGTCTTAAC GCAAATCCAG ATCATGTTGT ATTCACGTCT GGTGCAAGTG AAGCAGCCAT GACTTTATTA ACACCCTTTT ATAATATGGG AAGCTCTAAA GTTCAATTTT CCCATCTTTA CATTGGGGCA ACTGAACATC CATCTGTTGC AGAAGGTGGA CGTTTTCCTA AAGAATTCAT TAGTACAATT GCTGTTGATC AAGATGGACT TATTCAACAA GATAAATTGC TCTCTCTCTT AAGCTTTCAT GATAAAACAA AGGGACTTCC TCTTGTTGCT ATTCAAGCCG CAAATGGTGA AACGGGTGTT ATGCAACAAA TAAAAGAAAT AGCCGCTATT GTTAGGAACT GTGGAGGTAC TCTTATTGTT GATTTGACAC AGTATATAGA TAAAAATTTT GTTGATGTTC ATCAGATGGA AGGAGATTTT TTTATATTGT CTGCGCATAA AATTGGTGGT CCTAAAGGGA TTGGTGCTTT TGTTTCATGC GGAAACCTTT TGATGCCGTA TCCTCTTATT GTTGGAGGAG GGCAAGAAAA AGGTTTGCGT GGAGGAACAG AAGCATTGCC ACTTATTGCT GCTTTTGGGG CAGCAATGGC TGATTGCTAT AACCAAGAAG AAATAAAGCA ATTACTCTAT TTACGCCATA AATTAGAAGA TGGAATACAG AAAATATGTC ATGATGTTGA AATCTTTGGC AAAAAAGTTC AGCGTTTACC AAACACAACT TATTTCACTG TGCGCAACAT AAAAGCTGAA ACTTTACAAA TTAGTTTTGA TTTGGCAGGA TTTTCGGTAT CAGCAGGCTC TGCTTGCTCT TCAGGAAAAG TAAAACAAAG CCGTGTTTTG GAGGCAATGG GATATGATGT TCCAAATGGT GCAATTCGTA TTTCAACAGG ACGGTGCACG ACTTCCAAAG ATATTGATGA TTTTTTATTG TTTTTTTCTC AAATTATAAA TAAGAAAAAA GCTGGAACTT TA

Details for BaheA.01104.a.A1.GE26077

HARVESTED ON:	1/9/2009
SEQUENCED ON:	7/28/2010
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	80
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVVKRRYFD HNATTPLKKA AEVALLESLE IFGNPSSVHT EGRAAKALLQ KARRQIAKSL NANPDHVVFT SGASEAAMTL LTPFYNMGSS KVQFSHLYIG ATEHPSVAEG GRFPKEFIST IAVDQDGLIQ QDKLLSLLSF HDKTKGLPLV AIQAANGETG VMXQIKEIXA IVRNCGGTLI VDLTQYIXKN XVDVHXMXXX FFILSAHXIX XPKGIXAFVS CGNLLMPYPL IVGGGQEKGX RGGTXXXPXX XAXGAAXXXX XXXXXXXXXL XXXXXXEDGI QKXXHXXXXX XXXVQRXPNX XYFXVXXIKX ETLQIXXXLX GXSXXXGSAX XSGKXKQSRX LEAMXYDVXN XAIRISXGRX XTSKDIDDXX LFFS

Validated NT Sequence

taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gttgtaaaac gtcgatattt tgatcataac gcgacaacac cgcttaagaa ggcggcagag gtagcgttac tggaatcttt agagatcttt ggtaatccct catccgttca tacggaagga cgtgctgcta aagctttatt acaaaaggca cgtcgacaaa ttgctaaaag tcttaacgca aatccagatc atgttgtatt cacgtctggt gcaagtgaag cagccatgac tttattaaca cccttttata atatgggaag ctctaaagtt caattttccc atctttacat tggggcaact gaacatccat ctgttgcaga aggtggacgt tttcctaaag aattcattag tacaattgct gttgatcaag atggacttat tcaacaagat aaattgctct ctctcttaag ctttcatgat aaaacaaagg gacttcctct tgttgctatt caagccgcaa atggtgaaac gggtgttatg cnncaaataa aagaaatanc cgctattgtt aggaactgtg gaggtactct tattgttgat ttgacacagt atatanataa aaattntgtt gatgttcatc anatgnaang agantttttt atattgtctg cgcataanat tgntgntcct aaagggattg nngcttttgt ttcatgcgga aaccttttga tgccgtatcc tcttattgtt ggaggagggc aagaaaaagg ttngcgtgga ggaacagann natnnccnnt tannnnngct tnnggggcag caanggntnn ntnnnnnnnc nnngannnna nnnngnannn nctctannnn nnccannaat nagaagatgg aatacagaaa ntangncatg nnnnnnaant ctntngcaan naagttcagc gnttnccaaa cncannttat ttcnctgtgn gcaanataaa agnngaaact ttacaaattn gtttngnttt gnnnggannt tcnntannnn caggctctgc nngctnttca ggaaaantaa aacaaagccg tgnnttggag gcaatgngat atgatgttnc aaatnntgca attcgtattt cancaggncg gtgnnngact tccaaagata ttgatgattn tntattgttt ttttctcaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVVKRRYFD HNATTPLKKA AEVALLESLE IFGNPSSVHT EGRAAKALLQ KARRQIAKSL NANPDHVVFT SGASEAAMTL LTPFYNMGSS KVQFSHLYIG ATEHPSVAEG GRFPKEFIST IAVDQDGLIQ QDKLLSLLSF HDKTKGLPLV AIQAANGETG VMQQIKEIAA IVRNCGGTLI VDLTQYIDKN FVDVHQMEGD FFILSAHKIG GPKGIGAFVS CGNLLMPYPL IVGGGQEKGL RGGTEALPLI AAFGAAMADC YNQEEIKQLL YLRHKLEDGI QKICHDVEIF GKKVQRLPNT TYFTVRNIKA ETLQISFDLA GFSVSAGSAC SSGKVKQSRV LEAMGYDVPN GAIRISTGRC TTSKDIDDFL LFFSQIINKK KAGTL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgtaaaa cgtcgatatt ttgatcataa cgcgacaaca ccgcttaaga aggcggcaga ggtagcgtta ctggaatctt tagagatctt tggtaatccc tcatccgttc atacggaagg acgtgctgct aaagctttat tacaaaaggc acgtcgacaa attgctaaaa gtcttaacgc aaatccagat catgttgtat tcacgtctgg tgcaagtgaa gcagccatga ctttattaac acccttttat aatatgggaa gctctaaagt tcaattttcc catctttaca ttggggcaac tgaacatcca tctgttgcag aaggtggacg ttttcctaaa gaattcatta gtacaattgc tgttgatcaa gatggactta ttcaacaaga taaattgctc tctctcttaa gctttcatga taaaacaaag ggacttcctc ttgttgctat tcaagccgca aatggtgaaa cgggtgttat gcaacaaata aaagaaatag ccgctattgt taggaactgt ggaggtactc ttattgttga tttgacacag tatatagata aaaattttgt tgatgttcat cagatggaag gagatttttt tatattgtct gcgcataaaa ttggtggtcc taaagggatt ggtgcttttg tttcatgcgg aaaccttttg atgccgtatc ctcttattgt tggaggaggg caagaaaaag gtttgcgtgg aggaacagaa gcattgccac ttattgctgc ttttggggca gcaatggctg attgctataa ccaagaagaa ataaagcaat tactctattt acgccataaa ttagaagatg gaatacagaa aatatgtcat gatgttgaaa tctttggcaa aaaagttcag cgtttaccaa acacaactta tttcactgtg cgcaacataa aagctgaaac tttacaaatt agttttgatt tggcaggatt ttcggtatca gcaggctctg cttgctcttc aggaaaagta aaacaaagcc gtgttttgga ggcaatggga tatgatgttc caaatggtgc aattcgtatt tcaacaggac ggtgcacgac ttccaaagat attgatgatt ttttattgtt tttttctcaa attataaata agaaaaaagc tggaacttta aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BaheA.01104.a.A1.PS00209

PURIFICATION DATe:	3/20/2009
CONCENTRATION:	54mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	80
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVVKRRYFD HNATTPLKKA AEVALLESLE IFGNPSSVHT EGRAAKALLQ KARRQIAKSL NANPDHVVFT SGASEAAMTL LTPFYNMGSS KVQFSHLYIG ATEHPSVAEG GRFPKEFIST IAVDQDGLIQ QDKLLSLLSF HDKTKGLPLV AIQAANGETG VMQQIKEIAA IVRNCGGTLI VDLTQYIDKN FVDVHQMEGD FFILSAHKIG GPKGIGAFVS CGNLLMPYPL IVGGGQEKGL RGGTEALPLI AAFGAAMADC YNQEEIKQLL YLRHKLEDGI QKICHDVEIF GKKVQRLPNT TYFTVRNIKA ETLQISFDLA GFSVSAGSAC SSGKVKQSRV LEAMGYDVPN GAIRISTGRC TTSKDIDDFL LFFSQIINKK KAGTL

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGTAAAA CGTCGATATT TTGATCATAA CGCGACAACA CCGCTTAAGA AGGCGGCAGA GGTAGCGTTA CTGGAATCTT TAGAGATCTT TGGTAATCCC TCATCCGTTC ATACGGAAGG ACGTGCTGCT AAAGCTTTAT TACAAAAGGC ACGTCGACAA ATTGCTAAAA GTCTTAACGC AAATCCAGAT CATGTTGTAT TCACGTCTGG TGCAAGTGAA GCAGCCATGA CTTTATTAAC ACCCTTTTAT AATATGGGAA GCTCTAAAGT TCAATTTTCC CATCTTTACA TTGGGGCAAC TGAACATCCA TCTGTTGCAG AAGGTGGACG TTTTCCTAAA GAATTCATTA GTACAATTGC TGTTGATCAA GATGGACTTA TTCAACAAGA TAAATTGCTC TCTCTCTTAA GCTTTCATGA TAAAACAAAG GGACTTCCTC TTGTTGCTAT TCAAGCCGCA AATGGTGAAA CGGGTGTTAT GCAACAAATA AAAGAAATAG CCGCTATTGT TAGGAACTGT GGAGGTACTC TTATTGTTGA TTTGACACAG TATATAGATA AAAATTTTGT TGATGTTCAT CAGATGGAAG GAGATTTTTT TATATTGTCT GCGCATAAAA TTGGTGGTCC TAAAGGGATT GGTGCTTTTG TTTCATGCGG AAACCTTTTG ATGCCGTATC CTCTTATTGT TGGAGGAGGG CAAGAAAAAG GTTTGCGTGG AGGAACAGAA GCATTGCCAC TTATTGCTGC TTTTGGGGCA GCAATGGCTG ATTGCTATAA CCAAGAAGAA ATAAAGCAAT TACTCTATTT ACGCCATAAA TTAGAAGATG GAATACAGAA AATATGTCAT GATGTTGAAA TCTTTGGCAA AAAAGTTCAG CGTTTACCAA ACACAACTTA TTTCACTGTG CGCAACATAA AAGCTGAAAC TTTACAAATT AGTTTTGATT TGGCAGGATT TTCGGTATCA GCAGGCTCTG CTTGCTCTTC AGGAAAAGTA AAACAAAGCC GTGTTTTGGA GGCAATGGGA TATGATGTTC CAAATGGTGC AATTCGTATT TCAACAGGAC GGTGCACGAC TTCCAAAGAT ATTGATGATT TTTTATTGTT TTTTTCTCAA ATTATAAATA AGAAAAAAGC TGGAACTTTA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA