BaheA.01099.a

glutamyl-tRNA synthetase

CENTER ID: BaheA.01099.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01099.a.B1.GE43957 Full length( BaheA.01099.a ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.703
RefSeq: YP_033475.1
UniProt: Q6G3V1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKVRFAPSP TGYIHIGNIR IALFNWLYAQ AHNGTFILRY DNTDVERSKQ EYIDAIAVDL EWLGIQPDEI YYQSKRFNRY DEVAEILKQR GLLYPCYETA EELDRRRKIQ LSRKLPPVYD RAALKLTPEK KREFETQGRK PHWRFLLPNF ENDPLQKKRT EVCWNDAVKG KQTIDLASLS DPVLIREDGS YLYTLPSVVD DIDMAITHII RGDDHITNTG AQIALFEALN AKLPTFGHIN LLTTLLGKGL SKRNNDLSIH SLRADGFESI AVQCLAVLIG TSQNVHPYPN QAVLLEHFNL QDTSRSVAKF DIADLLTLNS HFVHELTYEE VKKRLENLSI NGEKVECFWN AIRSNINKVN DAVLWWKMLH DEQNFDTVAL EDRAFVRQSL NLLPEGTLNE ESWKVWTVAL KEKTGRRGKA LFMPLRQALT GMDHGPEMGK ILQLLGREKV IERLIIQGE
NT Sequence
ATGATAAAAG TTCGTTTTGC ACCTTCTCCA ACAGGTTATA TTCACATTGG CAATATCCGT ATTGCCTTGT TCAACTGGCT TTATGCGCAA GCGCATAATG GAACATTTAT TTTGCGCTAT GATAATACGG ATGTTGAACG TTCTAAACAG GAATATATTG ATGCTATTGC TGTTGATCTT GAATGGCTTG GTATTCAACC AGATGAGATT TATTATCAAT CTAAGAGATT TAATCGATAT GATGAAGTTG CAGAAATTCT CAAACAACGT GGTCTTCTTT ATCCCTGTTA TGAGACAGCA GAAGAATTAG ATCGACGTCG CAAAATCCAG CTTTCACGCA AATTGCCTCC TGTCTATGAC CGCGCTGCAT TGAAATTGAC ACCAGAAAAG AAAAGAGAAT TTGAAACACA AGGTCGTAAA CCGCATTGGC GTTTTCTTCT TCCTAATTTT GAAAATGATC CTTTACAAAA AAAGCGAACA GAAGTTTGCT GGAACGATGC AGTAAAGGGA AAGCAGACAA TAGATTTGGC TTCTCTTTCA GATCCTGTTC TCATTCGTGA AGATGGAAGC TACCTTTATA CACTGCCTTC TGTGGTTGAT GATATAGATA TGGCTATTAC GCATATTATT CGTGGGGATG ATCATATTAC AAATACAGGT GCGCAGATTG CTCTTTTTGA AGCCCTCAAT GCCAAATTAC CAACTTTTGG TCATATCAAT TTATTGACTA CGCTTTTAGG AAAAGGGCTC TCAAAACGAA ACAATGATCT TTCTATCCAC TCTCTACGGG CAGATGGATT TGAATCTATA GCTGTTCAGT GTCTTGCTGT TTTGATTGGA ACATCACAAA ATGTTCATCC TTATCCTAAT CAAGCAGTTC TTTTAGAGCA TTTCAATCTG CAAGATACGT CAAGATCGGT TGCAAAATTT GATATTGCTG ATCTTCTTAC TTTGAATAGT CATTTTGTCC ATGAGTTAAC CTATGAAGAA GTTAAAAAGC GTCTTGAAAA TCTTTCTATT AATGGAGAAA AGGTGGAGTG TTTTTGGAAC GCAATAAGAA GCAATATTAA TAAAGTGAAT GATGCTGTTT TGTGGTGGAA AATGCTTCAT GATGAACAGA ACTTTGATAC AGTGGCACTT GAGGATCGCG CATTCGTTCG TCAGTCACTT AATTTATTGC CCGAAGGTAC ATTGAATGAG GAAAGTTGGA AAGTTTGGAC GGTGGCATTA AAAGAAAAAA CAGGTCGTAG AGGAAAAGCT TTATTTATGC CCTTACGTCA GGCACTTACT GGAATGGATC ATGGACCTGA AATGGGAAAA ATTTTACAGC TCTTAGGGCG TGAAAAAGTA ATAGAAAGAC TGATTATACA AGGGGAA
Details for BaheA.01099.a.B1.GE43957
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 54kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI KVRFAPSPTG YIHIGNIRIA LFNWLYAQAH NGTFILRYDN TDVERSKQEY IDAIAVDLEW LGIQPDEIYY QSKRFNRYDE VAEILKQRGL LYPCYETAXE LDRRRKIQLS RKLPPVYDRA ALKLTPEKKR EFETQGRKPH WRFLLPNFEN DPLQKKRTEV CWNDAVKGKQ TIDLASLSDP VLIREDGSYL YTLPSVVDDI DMAITHIIRG DDHITNTGAQ IALFEALNAK LPTFGHINLL TTLLGKGLSK RNNDLSIHSL RADGFESIAV QCLAVLIGTS QNVHPYPNQA VLLEHFNLQD TSRSVAKFDI ADLLTLNSHF VHELTYEEVK KRLENLSING EKVECFWNAI RSNINKVNDA VLWWKMLHDE QNFDTVALED RAFVRQSLNL LPEGTLNEES WKVWTVALKE KTGRRGKALF MPLRQALTGM DHGPEMGKIL QLLGREKVIE
Validated NT Sequence
atggctcacc accaccacca ccatatgata aaagttcgtt ttgcaccttc tccaacaggt tatattcaca ttggcaatat ccgtattgcc ttgttcaact ggctttatgc gcaagcgcat aatggaacat ttattttgcg ctatgataat acggatgttg aacgttctaa acaggaatat attgatgcta ttgctgttga tcttgaatgg cttggtattc aaccagatga gatttattat caatctaaga gatttaatcg atatgatgaa gttgcagaaa ttctcaaaca acgtggtctt ctttatccct gttatgagac agcanaagaa ttagatcgac gtcgcaaaat ccagctttca cgcaaattgc ctcctgtcta tgaccgcgct gcattgaaat tgacaccaga aaagaaaaga gaatttgaaa cacaaggtcg taaaccgcat tggcgttttc ttcttcctaa ttttgaaaat gatcctttac aaaaaaagcg aacagaagtt tgctggaacg atgcagtaaa gggaaagcag acaatagatt tggcttctct ttcagatcct gttctcattc gtgaagatgg aagctacctt tatacactgc cttctgtggt tgatgatata gatatggcta ttacgcatat tattcgtggg gatgatcata ttacaaatac aggtgcgcag attgctcttt ttgaagccct caatgccaaa ttaccaactt ttggtcatat caatttattg actacgcttt taggaaaagg gctctcaaaa cgaaacaatg atctttctat ccactctcta cgggcagatg gatttgaatc tatagctgtt cagtgtcttg ctgttttgat tggaacatca caaaatgttc atccttatcc taatcaagca gttcttttag agcatttcaa tctgcaagat acgtcaagat cggttgcaaa atttgatatt gctgatcttc ttactttgaa tagtcatttt gtccatgagt taacctatga agaagttaaa aagcgtcttg aaaatctttc tattaatgga gaaaaggtgg agtgtttttg gaacgcaata agaagcaata ttaataaagt gaatgatgct gttttgtggt ggaaaatgct tcatgatgaa cagaactttg atacagtggc acttgaggat cgcgcattcg ttcgtcagtc acttaattta ttgcccgaag gtacattgaa tgaggaaagt tggaaagttt ggacggtggc attaaaagaa aaaacaggtc gtagaggaaa agctttattt atgcccttac gtcaggcact tactggaatg gatcatggac ctgaaatggg aaaaatttta cagctcttag ggcgtgaaaa agtaatagaa a
Expected Protein Sequence
MAHHHHHHMI KVRFAPSPTG YIHIGNIRIA LFNWLYAQAH NGTFILRYDN TDVERSKQEY IDAIAVDLEW LGIQPDEIYY QSKRFNRYDE VAEILKQRGL LYPCYETAEE LDRRRKIQLS RKLPPVYDRA ALKLTPEKKR EFETQGRKPH WRFLLPNFEN DPLQKKRTEV CWNDAVKGKQ TIDLASLSDP VLIREDGSYL YTLPSVVDDI DMAITHIIRG DDHITNTGAQ IALFEALNAK LPTFGHINLL TTLLGKGLSK RNNDLSIHSL RADGFESIAV QCLAVLIGTS QNVHPYPNQA VLLEHFNLQD TSRSVAKFDI ADLLTLNSHF VHELTYEEVK KRLENLSING EKVECFWNAI RSNINKVNDA VLWWKMLHDE QNFDTVALED RAFVRQSLNL LPEGTLNEES WKVWTVALKE KTGRRGKALF MPLRQALTGM DHGPEMGKIL QLLGREKVIE RLIIQGE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgataa aagttcgttt tgcaccttct ccaacaggtt atattcacat tggcaatatc cgtattgcct tgttcaactg gctttatgcg caagcgcata atggaacatt tattttgcgc tatgataata cggatgttga acgttctaaa caggaatata ttgatgctat tgctgttgat cttgaatggc ttggtattca accagatgag atttattatc aatctaagag atttaatcga tatgatgaag ttgcagaaat tctcaaacaa cgtggtcttc tttatccctg ttatgagaca gcagaagaat tagatcgacg tcgcaaaatc cagctttcac gcaaattgcc tcctgtctat gaccgcgctg cattgaaatt gacaccagaa aagaaaagag aatttgaaac acaaggtcgt aaaccgcatt ggcgttttct tcttcctaat tttgaaaatg atcctttaca aaaaaagcga acagaagttt gctggaacga tgcagtaaag ggaaagcaga caatagattt ggcttctctt tcagatcctg ttctcattcg tgaagatgga agctaccttt atacactgcc ttctgtggtt gatgatatag atatggctat tacgcatatt attcgtgggg atgatcatat tacaaataca ggtgcgcaga ttgctctttt tgaagccctc aatgccaaat taccaacttt tggtcatatc aatttattga ctacgctttt aggaaaaggg ctctcaaaac gaaacaatga tctttctatc cactctctac gggcagatgg atttgaatct atagctgttc agtgtcttgc tgttttgatt ggaacatcac aaaatgttca tccttatcct aatcaagcag ttcttttaga gcatttcaat ctgcaagata cgtcaagatc ggttgcaaaa tttgatattg ctgatcttct tactttgaat agtcattttg tccatgagtt aacctatgaa gaagttaaaa agcgtcttga aaatctttct attaatggag aaaaggtgga gtgtttttgg aacgcaataa gaagcaatat taataaagtg aatgatgctg ttttgtggtg gaaaatgctt catgatgaac agaactttga tacagtggca cttgaggatc gcgcattcgt tcgtcagtca cttaatttat tgcccgaagg tacattgaat gaggaaagtt ggaaagtttg gacggtggca ttaaaagaaa aaacaggtcg tagaggaaaa gctttattta tgcccttacg tcaggcactt actggaatgg atcatggacc tgaaatggga aaaattttac agctcttagg gcgtgaaaaa gtaatagaaa gactgattat acaaggggaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat