BaheA.01059.a

Phosphoribosylglycinamide formyltransferase

CENTER ID: BaheA.01059.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01059.a.A1.GE25990 Full length( BaheA.01059.a ) 1 203
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01059.a.A1.PS00161 Full length( BaheA.01059.a ) 1 203

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1001
RefSeq: YP_033752.1
UniProt: A0A0H3LWZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKQIVVFIS GNGSNMVALV KASKQKEYPA EIIAVICDNP HAKGIEKARD NHLPIHIIDR KDYPTKEAYE ESIFKVLAKY QPDLICFAGY MRLISSRFVK LYEGKILNIH PSLLPSFKGL KTHERVLQAG VKITGCTVHL VTEDMDSGKI LAQAAVPICP NDTADSLAQR VLKAEHKLYP EALKAFIEGN NKITDAQQQL LSF
NT Sequence
ATGAAAAAAC AAATTGTTGT TTTTATTTCC GGCAACGGCT CTAATATGGT TGCTCTGGTT AAAGCAAGCA AACAAAAAGA ATATCCTGCT GAAATCATCG CAGTTATTTG CGATAATCCT CATGCTAAAG GCATTGAAAA AGCACGTGAC AATCATTTGC CCATTCATAT TATTGATCGC AAGGATTATC CAACTAAAGA AGCATATGAA GAATCTATCT TTAAGGTTTT AGCCAAATAT CAGCCGGATC TTATCTGCTT TGCTGGTTAC ATGCGCCTCA TCTCATCTCG TTTCGTAAAA CTCTATGAAG GAAAAATTTT AAACATTCAC CCTTCTCTTT TGCCTTCATT TAAAGGTCTA AAAACACATG AACGAGTTTT ACAAGCAGGT GTTAAAATCA CTGGTTGCAC TGTTCACCTT GTTACAGAAG ATATGGATTC AGGTAAGATT CTTGCCCAAG CAGCTGTTCC AATATGTCCT AATGATACAG CCGATAGCTT AGCGCAAAGA GTTCTTAAGG CTGAACATAA ACTCTACCCC GAAGCTTTAA AAGCATTCAT TGAAGGAAAC AACAAAATAA CAGATGCTCA ACAACAACTT TTATCCTTT
Details for BaheA.01059.a.A1.GE25990
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 24kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKKQIVVFI SGNGSNMVAL VKASKQKEYP AEIIAVICDN PHAKGIEKAR DNHLPIHIID RKDYPTKEAY EESIFKVLAK YQPDLICFAG YMRLISSRFV KLYEGKILNI HPSLLPSFKG LKTHERVLQA GVKITGCTVH LVTEDMDSGK ILAQAAVPIC PNDTADSLAQ RVLKAEHKLY PEALKAFIEG NNKITDAQQQ LLSF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa aacaaattgt tgtttttatt tccggcaacg gctctaatat ggttgctctg gttaaagcaa gcaaacaaaa agaatatcct gctgaaatca tcgcagttat ttgcgataat cctcatgcta aaggcattga aaaagcacgt gacaatcatt tgcccattca tattattgat cgcaaggatt atccaactaa agaagcatat gaagaatcta tctttaaggt tttagccaaa tatcagccgg atcttatctg ctttgctggt tacatgcgcc tcatctcatc tcgtttcgta aaactctatg aaggaaaaat tttaaacatt cacccttctc ttttgccttc atttaaaggt ctaaaaacac atgaacgagt tttacaagca ggtgttaaaa tcactggttg cactgttcac cttgttacag aagatatgga ttcaggtaag attcttgccc aagcagctgt tccaatatgt cctaatgata cagccgatag cttagcgcaa agagttctta aggctgaaca taaactctac cccgaagctt taaaagcatt cattgaagga aacaacaaaa taacagatgc tcaacaacaa cttttatcct tttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKQIVVFI SGNGSNMVAL VKASKQKEYP AEIIAVICDN PHAKGIEKAR DNHLPIHIID RKDYPTKEAY EESIFKVLAK YQPDLICFAG YMRLISSRFV KLYEGKILNI HPSLLPSFKG LKTHERVLQA GVKITGCTVH LVTEDMDSGK ILAQAAVPIC PNDTADSLAQ RVLKAEHKLY PEALKAFIEG NNKITDAQQQ LLSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaaaacaa attgttgttt ttatttccgg caacggctct aatatggttg ctctggttaa agcaagcaaa caaaaagaat atcctgctga aatcatcgca gttatttgcg ataatcctca tgctaaaggc attgaaaaag cacgtgacaa tcatttgccc attcatatta ttgatcgcaa ggattatcca actaaagaag catatgaaga atctatcttt aaggttttag ccaaatatca gccggatctt atctgctttg ctggttacat gcgcctcatc tcatctcgtt tcgtaaaact ctatgaagga aaaattttaa acattcaccc ttctcttttg ccttcattta aaggtctaaa aacacatgaa cgagttttac aagcaggtgt taaaatcact ggttgcactg ttcaccttgt tacagaagat atggattcag gtaagattct tgcccaagca gctgttccaa tatgtcctaa tgatacagcc gatagcttag cgcaaagagt tcttaaggct gaacataaac tctaccccga agctttaaaa gcattcattg aaggaaacaa caaaataaca gatgctcaac aacaactttt atcctttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BaheA.01059.a.A1.PS00161
PURIFICATION DATe: 2/11/2009
CONCENTRATION: 47mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKKQIVVFI SGNGSNMVAL VKASKQKEYP AEIIAVICDN PHAKGIEKAR DNHLPIHIID RKDYPTKEAY EESIFKVLAK YQPDLICFAG YMRLISSRFV KLYEGKILNI HPSLLPSFKG LKTHERVLQA GVKITGCTVH LVTEDMDSGK ILAQAAVPIC PNDTADSLAQ RVLKAEHKLY PEALKAFIEG NNKITDAQQQ LLSF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa aacaaattgt tgtttttatt tccggcaacg gctctaatat ggttgctctg gttaaagcaa gcaaacaaaa agaatatcct gctgaaatca tcgcagttat ttgcgataat cctcatgcta aaggcattga aaaagcacgt gacaatcatt tgcccattca tattattgat cgcaaggatt atccaactaa agaagcatat gaagaatcta tctttaaggt tttagccaaa tatcagccgg atcttatctg ctttgctggt tacatgcgcc tcatctcatc tcgtttcgta aaactctatg aaggaaaaat tttaaacatt cacccttctc ttttgccttc atttaaaggt ctaaaaacac atgaacgagt tttacaagca ggtgttaaaa tcactggttg cactgttcac cttgttacag aagatatgga ttcaggtaag attcttgccc aagcagctgt tccaatatgt cctaatgata cagccgatag cttagcgcaa agagttctta aggctgaaca taaactctac cccgaagctt taaaagcatt cattgaagga aacaacaaaa taacagatgc tcaacaacaa cttttatcct tttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKQIVVFI SGNGSNMVAL VKASKQKEYP AEIIAVICDN PHAKGIEKAR DNHLPIHIID RKDYPTKEAY EESIFKVLAK YQPDLICFAG YMRLISSRFV KLYEGKILNI HPSLLPSFKG LKTHERVLQA GVKITGCTVH LVTEDMDSGK ILAQAAVPIC PNDTADSLAQ RVLKAEHKLY PEALKAFIEG NNKITDAQQQ LLSF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAAAACAA ATTGTTGTTT TTATTTCCGG CAACGGCTCT AATATGGTTG CTCTGGTTAA AGCAAGCAAA CAAAAAGAAT ATCCTGCTGA AATCATCGCA GTTATTTGCG ATAATCCTCA TGCTAAAGGC ATTGAAAAAG CACGTGACAA TCATTTGCCC ATTCATATTA TTGATCGCAA GGATTATCCA ACTAAAGAAG CATATGAAGA ATCTATCTTT AAGGTTTTAG CCAAATATCA GCCGGATCTT ATCTGCTTTG CTGGTTACAT GCGCCTCATC TCATCTCGTT TCGTAAAACT CTATGAAGGA AAAATTTTAA ACATTCACCC TTCTCTTTTG CCTTCATTTA AAGGTCTAAA AACACATGAA CGAGTTTTAC AAGCAGGTGT TAAAATCACT GGTTGCACTG TTCACCTTGT TACAGAAGAT ATGGATTCAG GTAAGATTCT TGCCCAAGCA GCTGTTCCAA TATGTCCTAA TGATACAGCC GATAGCTTAG CGCAAAGAGT TCTTAAGGCT GAACATAAAC TCTACCCCGA AGCTTTAAAA GCATTCATTG AAGGAAACAA CAAAATAACA GATGCTCAAC AACAACTTTT ATCCTTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA