BaheA.01050.a

GTP cyclohydrolase II

CENTER ID: BaheA.01050.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01050.a.A1.GE25997 Full length( BaheA.01050.a ) 1 368
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.01050.a.A1.PS00160 Full length( BaheA.01050.a ) 1 368
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127275
PATRIC ID: fig|283166.5.peg.474
RefSeq: YP_033271.1
UniProt: A0A0H3LXQ3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYDQKKIVS ALRAFERGEI VVVTDDDDRE NEGDLIVAAV HCTEEKMAFI IRHTTGIVCA PMPKEEAQRF NLTPMVLDNN SVHRTQFTVT VDFKHGTTTG ISAHDRTLAV RNLANSNAGA NDFIRPGHIF PLIAHEGGVL MRSGHTEAAV DLCKLAGLPP VGVIGELVND DGSVKHGDEV TRFAQENQFH IITVADLIAY RQRKEMLIQH VGEMHIETLV GPAVVQSYQL PWEAVQHVAI IFGDIRDGED IPVRLHRENI VNDVFGQTSD ILEIMRRMMK KEKRGVFVYL REGSVGVKSA VSIQALAMRG GESHTQAIER EEEWRQIGLG SQILKHLGIS SVILYASKER HYVGLEGFGI RISRTDIL
NT Sequence
ATGACGTATG ATCAAAAAAA AATTGTTTCT GCACTTCGCG CTTTTGAACG CGGTGAAATT GTGGTGGTTA CAGATGATGA TGATCGTGAA AACGAAGGTG ATTTGATTGT TGCCGCTGTC CATTGCACAG AAGAAAAGAT GGCATTTATC ATTCGCCATA CAACGGGTAT TGTTTGTGCT CCTATGCCAA AAGAAGAAGC GCAACGGTTT AATCTTACTC CTATGGTGTT AGATAATAAT TCAGTGCATC GTACCCAATT TACTGTGACT GTTGATTTTA AACATGGAAC AACGACAGGG ATTTCAGCAC ACGACCGTAC ATTAGCGGTG CGTAATCTTG CTAATTCGAA TGCTGGTGCG AACGATTTTA TCCGTCCAGG ACATATTTTC CCATTGATTG CGCATGAAGG GGGAGTTCTC ATGCGTTCAG GCCACACGGA GGCAGCTGTT GATTTATGTA AATTGGCGGG TTTGCCACCA GTTGGCGTTA TTGGTGAATT GGTCAATGAT GACGGTAGCG TGAAACATGG AGATGAAGTT ACAAGATTTG CACAAGAGAA TCAGTTTCAT ATCATAACAG TTGCAGATTT GATTGCTTAT CGCCAACGTA AGGAAATGTT GATCCAACAT GTTGGAGAAA TGCATATTGA AACATTAGTA GGACCAGCTG TCGTTCAAAG TTATCAACTT CCTTGGGAAG CTGTTCAACA TGTTGCAATC ATTTTTGGTG ATATCCGTGA CGGTGAGGAT ATTCCTGTGC GCTTGCATCG TGAAAATATT GTGAACGATG TTTTTGGTCA AACTTCAGAT ATTTTAGAAA TCATGCGGCG TATGATGAAA AAAGAAAAAC GTGGTGTGTT TGTTTATTTG CGTGAAGGAT CTGTTGGTGT AAAATCCGCT GTTAGTATTC AGGCTCTAGC AATGAGGGGG GGAGAAAGCC ATACGCAAGC GATTGAACGC GAAGAAGAAT GGCGCCAAAT TGGTTTAGGA TCACAAATTT TAAAGCATTT AGGGATTAGT TCTGTTATCC TTTATGCTTC TAAAGAGCGT CATTATGTAG GTTTGGAAGG TTTTGGAATT CGTATATCCA GAACAGATAT CTTG
Details for BaheA.01050.a.A1.GE25997
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYDQKKIV SALRAFERGE IVVVTDDDDR ENEGDLIVAA VHCTEEKMAF IIRHTTGIVC APMPKEEAQR FNLTPMVLDN NSVHRTQFTV TVDFKHGTTT GISAHDRTLA VRNLANSNAG ANDFIRPGHI FPLIAHEGGV LMRSGHTEAA VDLCKLAGLP PVGVIGELVN DDGSVKHGDE VTRFAQENQF HIITVADLIA YRQRKEMLIQ HVGEMHIETL VGPAVVQSYQ LPWEAVQHVA IIFGDIRDGE DIPVRLHREN IVNDVFGQTS DILEIMRRMM KKEKRGVFVY LREGSVGVKS AVSIQALAMR GGESHTQAIE REEEWRQIGL GSQILKHLGI SSVILYASKE RHYVGLEGFG IRISRTDIL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgt atgatcaaaa aaaaattgtt tctgcacttc gcgcttttga acgcggtgaa attgtggtgg ttacagatga tgatgatcgt gaaaacgaag gtgatttgat tgttgccgct gtccattgca cagaagaaaa gatggcattt atcattcgcc atacaacggg tattgtttgt gctcctatgc caaaagaaga agcgcaacgg tttaatctta ctcctatggt gttagataat aattcagtgc atcgtaccca atttactgtg actgttgatt ttaaacatgg aacaacgaca gggatttcag cacacgaccg tacattagcg gtgcgtaatc ttgctaattc gaatgctggt gcgaacgatt ttatccgtcc aggacatatt ttcccattga ttgcgcatga agggggagtt ctcatgcgtt caggccacac ggaggcagct gttgatttat gtaaattggc gggtttgcca ccagttggcg ttattggtga attggtcaat gatgacggta gcgtgaaaca tggagatgaa gttacaagat ttgcacaaga gaatcagttt catatcataa cagttgcaga tttgattgct tatcgccaac gtaaggaaat gttgatccaa catgttggag aaatgcatat tgaaacatta gtaggaccag ctgtcgttca aagttatcaa cttccttggg aagctgttca acatgttgca atcatttttg gtgatatccg tgacggtgag gatattcctg tgcgcttgca tcgtgaaaat attgtgaacg atgtttttgg tcaaacttca gatattttag aaatcatgcg gcgtatgatg aaaaaagaaa aacgtggtgt gtttgtttat ttgcgtgaag gatctgttgg tgtaaaatcc gctgttagta ttcaggctct agcaatgagg gggggagaaa gccatacgca agcgattgaa cgcgaagaag aatggcgcca aattggttta ggatcacaaa ttttaaagca tttagggatt agttctgtta tcctttatgc ttctaaagag cgtcattatg taggtttgga aggttttgga attcgtatat ccagaacaga tatcttgtaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYDQKKIV SALRAFERGE IVVVTDDDDR ENEGDLIVAA VHCTEEKMAF IIRHTTGIVC APMPKEEAQR FNLTPMVLDN NSVHRTQFTV TVDFKHGTTT GISAHDRTLA VRNLANSNAG ANDFIRPGHI FPLIAHEGGV LMRSGHTEAA VDLCKLAGLP PVGVIGELVN DDGSVKHGDE VTRFAQENQF HIITVADLIA YRQRKEMLIQ HVGEMHIETL VGPAVVQSYQ LPWEAVQHVA IIFGDIRDGE DIPVRLHREN IVNDVFGQTS DILEIMRRMM KKEKRGVFVY LREGSVGVKS AVSIQALAMR GGESHTQAIE REEEWRQIGL GSQILKHLGI SSVILYASKE RHYVGLEGFG IRISRTDIL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgtatgat caaaaaaaaa ttgtttctgc acttcgcgct tttgaacgcg gtgaaattgt ggtggttaca gatgatgatg atcgtgaaaa cgaaggtgat ttgattgttg ccgctgtcca ttgcacagaa gaaaagatgg catttatcat tcgccataca acgggtattg tttgtgctcc tatgccaaaa gaagaagcgc aacggtttaa tcttactcct atggtgttag ataataattc agtgcatcgt acccaattta ctgtgactgt tgattttaaa catggaacaa cgacagggat ttcagcacac gaccgtacat tagcggtgcg taatcttgct aattcgaatg ctggtgcgaa cgattttatc cgtccaggac atattttccc attgattgcg catgaagggg gagttctcat gcgttcaggc cacacggagg cagctgttga tttatgtaaa ttggcgggtt tgccaccagt tggcgttatt ggtgaattgg tcaatgatga cggtagcgtg aaacatggag atgaagttac aagatttgca caagagaatc agtttcatat cataacagtt gcagatttga ttgcttatcg ccaacgtaag gaaatgttga tccaacatgt tggagaaatg catattgaaa cattagtagg accagctgtc gttcaaagtt atcaacttcc ttgggaagct gttcaacatg ttgcaatcat ttttggtgat atccgtgacg gtgaggatat tcctgtgcgc ttgcatcgtg aaaatattgt gaacgatgtt tttggtcaaa cttcagatat tttagaaatc atgcggcgta tgatgaaaaa agaaaaacgt ggtgtgtttg tttatttgcg tgaaggatct gttggtgtaa aatccgctgt tagtattcag gctctagcaa tgaggggggg agaaagccat acgcaagcga ttgaacgcga agaagaatgg cgccaaattg gtttaggatc acaaatttta aagcatttag ggattagttc tgttatcctt tatgcttcta aagagcgtca ttatgtaggt ttggaaggtt ttggaattcg tatatccaga acagatatct tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BaheA.01050.a.A1.PS00160
PURIFICATION DATe: 3/5/2009
CONCENTRATION: 3.54mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYDQKKIV SALRAFERGE IVVVTDDDDR ENEGDLIVAA VHCTEEKMAF IIRHTTGIVC APMPKEEAQR FNLTPMVLDN NSVHRTQFTV TVDFKHGTTT GISAHDRTLA VRNLANSNAG ANDFIRPGHI FPLIAHEGGV LMRSGHTEAA VDLCKLAGLP PVGVIGELVN DDGSVKHGDE VTRFAQENQF HIITVADLIA YRQRKEMLIQ HVGEMHIETL VGPAVVQSYQ LPWEAVQHVA IIFGDIRDGE DIPVRLHREN IVNDVFGQTS DILEIMRRMM KKEKRGVFVY LREGSVGVKS AVSIQALAMR GGESHTQAIE REEEWRQIGL GSQILKHLGI SSVILYASKE RHYVGLEGFG IRISRTDIL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgt atgatcaaaa aaaaattgtt tctgcacttc gcgcttttga acgcggtgaa attgtggtgg ttacagatga tgatgatcgt gaaaacgaag gtgatttgat tgttgccgct gtccattgca cagaagaaaa gatggcattt atcattcgcc atacaacggg tattgtttgt gctcctatgc caaaagaaga agcgcaacgg tttaatctta ctcctatggt gttagataat aattcagtgc atcgtaccca atttactgtg actgttgatt ttaaacatgg aacaacgaca gggatttcag cacacgaccg tacattagcg gtgcgtaatc ttgctaattc gaatgctggt gcgaacgatt ttatccgtcc aggacatatt ttcccattga ttgcgcatga agggggagtt ctcatgcgtt caggccacac ggaggcagct gttgatttat gtaaattggc gggtttgcca ccagttggcg ttattggtga attggtcaat gatgacggta gcgtgaaaca tggagatgaa gttacaagat ttgcacaaga gaatcagttt catatcataa cagttgcaga tttgattgct tatcgccaac gtaaggaaat gttgatccaa catgttggag aaatgcatat tgaaacatta gtaggaccag ctgtcgttca aagttatcaa cttccttggg aagctgttca acatgttgca atcatttttg gtgatatccg tgacggtgag gatattcctg tgcgcttgca tcgtgaaaat attgtgaacg atgtttttgg tcaaacttca gatattttag aaatcatgcg gcgtatgatg aaaaaagaaa aacgtggtgt gtttgtttat ttgcgtgaag gatctgttgg tgtaaaatcc gctgttagta ttcaggctct agcaatgagg gggggagaaa gccatacgca agcgattgaa cgcgaagaag aatggcgcca aattggttta ggatcacaaa ttttaaagca tttagggatt agttctgtta tcctttatgc ttctaaagag cgtcattatg taggtttgga aggttttgga attcgtatat ccagaacaga tatcttgtaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYDQKKIV SALRAFERGE IVVVTDDDDR ENEGDLIVAA VHCTEEKMAF IIRHTTGIVC APMPKEEAQR FNLTPMVLDN NSVHRTQFTV TVDFKHGTTT GISAHDRTLA VRNLANSNAG ANDFIRPGHI FPLIAHEGGV LMRSGHTEAA VDLCKLAGLP PVGVIGELVN DDGSVKHGDE VTRFAQENQF HIITVADLIA YRQRKEMLIQ HVGEMHIETL VGPAVVQSYQ LPWEAVQHVA IIFGDIRDGE DIPVRLHREN IVNDVFGQTS DILEIMRRMM KKEKRGVFVY LREGSVGVKS AVSIQALAMR GGESHTQAIE REEEWRQIGL GSQILKHLGI SSVILYASKE RHYVGLEGFG IRISRTDIL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGTATGAT CAAAAAAAAA TTGTTTCTGC ACTTCGCGCT TTTGAACGCG GTGAAATTGT GGTGGTTACA GATGATGATG ATCGTGAAAA CGAAGGTGAT TTGATTGTTG CCGCTGTCCA TTGCACAGAA GAAAAGATGG CATTTATCAT TCGCCATACA ACGGGTATTG TTTGTGCTCC TATGCCAAAA GAAGAAGCGC AACGGTTTAA TCTTACTCCT ATGGTGTTAG ATAATAATTC AGTGCATCGT ACCCAATTTA CTGTGACTGT TGATTTTAAA CATGGAACAA CGACAGGGAT TTCAGCACAC GACCGTACAT TAGCGGTGCG TAATCTTGCT AATTCGAATG CTGGTGCGAA CGATTTTATC CGTCCAGGAC ATATTTTCCC ATTGATTGCG CATGAAGGGG GAGTTCTCAT GCGTTCAGGC CACACGGAGG CAGCTGTTGA TTTATGTAAA TTGGCGGGTT TGCCACCAGT TGGCGTTATT GGTGAATTGG TCAATGATGA CGGTAGCGTG AAACATGGAG ATGAAGTTAC AAGATTTGCA CAAGAGAATC AGTTTCATAT CATAACAGTT GCAGATTTGA TTGCTTATCG CCAACGTAAG GAAATGTTGA TCCAACATGT TGGAGAAATG CATATTGAAA CATTAGTAGG ACCAGCTGTC GTTCAAAGTT ATCAACTTCC TTGGGAAGCT GTTCAACATG TTGCAATCAT TTTTGGTGAT ATCCGTGACG GTGAGGATAT TCCTGTGCGC TTGCATCGTG AAAATATTGT GAACGATGTT TTTGGTCAAA CTTCAGATAT TTTAGAAATC ATGCGGCGTA TGATGAAAAA AGAAAAACGT GGTGTGTTTG TTTATTTGCG TGAAGGATCT GTTGGTGTAA AATCCGCTGT TAGTATTCAG GCTCTAGCAA TGAGGGGGGG AGAAAGCCAT ACGCAAGCGA TTGAACGCGA AGAAGAATGG CGCCAAATTG GTTTAGGATC ACAAATTTTA AAGCATTTAG GGATTAGTTC TGTTATCCTT TATGCTTCTA AAGAGCGTCA TTATGTAGGT TTGGAAGGTT TTGGAATTCG TATATCCAGA ACAGATATCT TGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA