BaheA.01015.a
tRNA (guanine-n1)-methyltransferase
| CENTER ID: | BaheA.01015.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.01015.a.A1.GE25996 | Full length( BaheA.01015.a ) | 1 | 232 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3IEF
DEPOSITED: 7/22/2009
DETERMINATION: XRay
CLONE: BaheA.01015.a.A1.GE25996
PROTEIN: BaheA.01015.a.A1.PW25986
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132581 |
| PATRIC ID: | fig|283166.5.peg.1838 |
| RefSeq: | YP_034278.1 |
| UniProt: | Q6G1R9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFQARVLTL YPEMFPGFLG CSLAGQALKQ GIWSLETVQI RDFALDKHHS VDDTPAGGGA GMVMRADVLA AALDSCPNDS PRLLMSPRGR LLNQAYARSL ARSSGVTLVC GRFEGVDERI IEARELEEVS IGDYILSGGE TAALVLLDAI VRLLPGVMGN EISAKCESFE NGLLEHPQYT RPAVFEGRGI PPVLTSGHHK AIANWRQQQA ESLTRQRRPD LYALYNKNRQ KT
NT Sequence
ATGAAATTTC AAGCACGTGT TTTAACACTT TATCCTGAAA TGTTTCCTGG TTTTTTGGGT TGTTCTTTAG CAGGGCAAGC TTTAAAGCAG GGGATATGGT CGCTTGAGAC AGTGCAGATT AGGGATTTTG CTCTTGATAA ACATCATAGC GTTGATGATA CGCCTGCTGG TGGTGGTGCT GGTATGGTGA TGCGGGCTGA TGTGTTAGCG GCGGCACTGG ATAGTTGTCC AAATGATTCC CCAAGATTGT TAATGAGCCC GCGTGGGAGA CTCTTGAATC AGGCTTATGC ACGTTCTTTG GCACGGTCTT CGGGAGTGAC ATTGGTTTGT GGGCGTTTTG AAGGAGTCGA TGAGCGGATA ATAGAAGCAC GAGAATTAGA AGAAGTGTCC ATTGGTGATT ATATTCTTTC AGGGGGCGAA ACGGCGGCAC TGGTTCTTTT GGATGCTATT GTGCGTCTTC TTCCCGGTGT TATGGGTAAT GAGATCTCCG CAAAGTGTGA AAGTTTTGAG AATGGTTTGC TTGAGCACCC TCAATATACG CGTCCTGCTG TTTTTGAAGG GCGTGGTATT CCGCCAGTGT TGACGTCAGG TCATCACAAA GCTATCGCCA ATTGGCGTCA ACAGCAAGCC GAATCGTTAA CACGGCAACG TCGCCCTGAT CTTTACGCTC TTTATAATAA AAATCGTCAG AAAACT
Details for BaheA.01015.a.A1.GE25996
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFQARVLT LYPEMFPGFL GCSLAGQALK QGIWSLETVQ IRDFALDKHH SVDDTPAGGG AGMVMRADVL AAALDSCPND SPRLLMSPRG RLLNQAYARS LARSSGVTLV CGRFEGVDER IIEARELEEV SIGDYILSGG ETAALVLLDA IVRLLPGVMG NEISAKCESF ENGLLEHPQY TRPAVFEGRG IPPVLTSGHH KAIANWRQQQ AESLTRQRRP DLYALYNKNR Q
Validated NT Sequence
tctgacgatt tttattataa agagcgtaaa gatcagggcg acgttgccgt gttaacgatt cggcttgctg ttgacgccaa ttggcgatag ctttgtgatg acctgacgtc aacactggcg gaataccacg cccttcaaaa acagcaggac gcgtatattg agggtgctca agcaaaccat tctcaaaact ttcacacttt gcggagatct cattacccat aacaccggga agaagacgca caatagcatc caaaagaacc agtgccgccg tttcgccccc tgaaagaata taatcaccaa tggacacttc ttctaattct cgtgcttcta ttatccgctc atcgactcct tcaaaacgcc cacaaaccaa tgtcactccc gaagaccgtg ccaaagaacg tgcataagcc tgattcaaga gtctcccacg cgggctcatt aacaatcttg gggaatcatt tggacaacta tccagtgccg ccgctaacac atcagcccgc atcaccatac cagcaccacc accagcaggc gtatcatcaa cgctatgatg tttatcaaga gcaaaatccc taatctgcac tgtctcaagc gaccatatcc cctgctttaa agcttgccct gctaaagaac aacccaaaaa accaggaaac atttcaggat aaagtgttaa aacacgtgct tgaaatttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFQARVLT LYPEMFPGFL GCSLAGQALK QGIWSLETVQ IRDFALDKHH SVDDTPAGGG AGMVMRADVL AAALDSCPND SPRLLMSPRG RLLNQAYARS LARSSGVTLV CGRFEGVDER IIEARELEEV SIGDYILSGG ETAALVLLDA IVRLLPGVMG NEISAKCESF ENGLLEHPQY TRPAVFEGRG IPPVLTSGHH KAIANWRQQQ AESLTRQRRP DLYALYNKNR QKT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatttcaa gcacgtgttt taacacttta tcctgaaatg tttcctggtt ttttgggttg ttctttagca gggcaagctt taaagcaggg gatatggtcg cttgagacag tgcagattag ggattttgct cttgataaac atcatagcgt tgatgatacg cctgctggtg gtggtgctgg tatggtgatg cgggctgatg tgttagcggc ggcactggat agttgtccaa atgattcccc aagattgtta atgagcccgc gtgggagact cttgaatcag gcttatgcac gttctttggc acggtcttcg ggagtgacat tggtttgtgg gcgttttgaa ggagtcgatg agcggataat agaagcacga gaattagaag aagtgtccat tggtgattat attctttcag ggggcgaaac ggcggcactg gttcttttgg atgctattgt gcgtcttctt cccggtgtta tgggtaatga gatctccgca aagtgtgaaa gttttgagaa tggtttgctt gagcaccctc aatatacgcg tcctgctgtt tttgaagggc gtggtattcc gccagtgttg acgtcaggtc atcacaaagc tatcgccaat tggcgtcaac agcaagccga atcgttaaca cggcaacgtc gccctgatct ttacgctctt tataataaaa atcgtcagaa aactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa