BaheA.00996.a

pantothenate kinase

CENTER ID: BaheA.00996.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00996.a.B1.GE26935 Full length( BaheA.00996.a ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00996.a.B1.PW26915 Full length( BaheA.00996.a ) 1 332

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.234
RefSeq: YP_033058.1
UniProt: A0A0H3LXA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDTILPKIN QEDNLVKSIS DRYSPYRVFT AQEWSQFRAD TPLTLTFDEI KRLRSIDDPI DLEEVQRIYL SLSRLLSCHV EAVQELFHKR QQFLYQEGTR KTPFIIGIAG SVAVGKSTTA RILQELLKRW TSSLKVDLIT TDGFLYPNAV LQQKNRMNRK GFPDSYDIKK LLCFLSAIKA GIGNVRAPLY SHMIYDVLEN QTVTIDRPDI LIVEGINVLQ VNDLPKDGKI IPFVSDFFDF SIYVDAETEV IRHWYLERFK RLRKTAFQNP KSYFHRYALM HEEESLKIAE NIWQTINLKN LQENILPTRP RSNLILRKGK KHLVEYVALR RL
NT Sequence
ATGATTGATA CAATTCTCCC AAAAATTAAT CAGGAAGATA ATTTAGTCAA ATCGATTTCC GATCGATATT CGCCCTATCG TGTCTTTACT GCACAAGAAT GGTCTCAATT TCGTGCAGAT ACACCGCTTA CTTTGACCTT TGATGAAATC AAACGTTTAC GCTCAATTGA TGATCCAATT GATCTTGAAG AAGTGCAACG TATTTATCTT TCTTTATCAC GTTTGTTATC ATGCCATGTT GAAGCTGTAC AAGAACTTTT TCACAAGCGT CAACAATTTT TATATCAAGA AGGAACGAGA AAAACTCCTT TTATCATTGG AATTGCTGGC TCTGTTGCAG TAGGAAAATC AACTACCGCT CGTATTCTAC AAGAACTTTT GAAACGTTGG ACTTCCAGTC TTAAAGTTGA TTTGATTACA ACGGATGGTT TTCTCTATCC TAACGCTGTT TTACAGCAAA AAAATCGTAT GAATCGTAAA GGATTTCCAG ATTCCTATGA TATTAAGAAA TTACTGTGCT TTCTTTCTGC TATAAAAGCC GGTATTGGTA ATGTACGTGC CCCACTTTAT TCGCATATGA TTTATGATGT TTTAGAAAAT CAAACAGTTA CCATTGATCG TCCCGATATC TTAATTGTTG AAGGGATTAA CGTGTTACAA GTCAATGACC TCCCTAAAGA CGGTAAAATT ATTCCTTTTG TTTCAGATTT TTTTGATTTT TCAATCTATG TAGATGCTGA AACAGAAGTC ATTCGACACT GGTATTTGGA ACGTTTTAAA CGTTTGCGTA AAACAGCTTT TCAAAATCCT AAGTCCTATT TTCATCGTTA TGCACTCATG CATGAAGAAG AGTCCTTAAA AATCGCTGAA AATATCTGGC AAACAATTAA TTTGAAAAAT TTACAAGAAA ATATATTGCC AACACGACCA CGGTCTAATC TCATTCTACG CAAGGGAAAA AAACATTTGG TTGAATATGT GGCACTTCGA CGACTA
Details for BaheA.00996.a.B1.GE26935
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI DTILPKINQE DNLVKSISDR YSPYRVFTAQ EWSQFRADTP LTLTFDEIKR LRSIDDPIDL EEVQRIYLSL SRLLSCHVEA VQELFHKRQQ FLYQEGTRKT PFIIGIAGSV AVGKSTTARI LQELLKRWTS SLKVDLITTD GFLYPNAVLQ QKNRMNRKGF PDSYDIKKLL CFLSAIKAGI GNVRAPLYSH MIYDVLENQT VTIDRPDILI VEGINVLQVN DLPKDGKIIP FVSDFFDFSI YVDAETEVIR HWYLERFKRL RKTAFQNPKS YFHRYALMHE EESLKIAENI WQTINLKNLQ ENISPTRPRS NLILRKGKKH LVEYVALRRL
Validated NT Sequence
atggctcacc accaccacca ccatatgatt gatacaattc tcccaaaaat taatcaggaa gataatttag tcaaatcgat ttccgatcga tattcgccct atcgtgtctt tactgcacaa gaatggtctc aatttcgtgc agatacaccg cttactttga cctttgatga aatcaaacgt ttacgctcaa ttgatgatcc aattgatctt gaagaagtgc aacgtattta tctttcttta tcacgtttgt tatcatgcca tgttgaagct gtacaagaac tttttcacaa gcgtcaacaa tttttatatc aagaaggaac gagaaaaact ccttttatca ttggaattgc tggctctgtt gcagtaggaa aatcaactac cgctcgtatt ctacaagaac ttttgaaacg ttggacttcc agtcttaaag ttgatttgat tacaacggat ggttttctct atcctaacgc tgttttacag caaaaaaatc gtatgaatcg taaaggattt ccagattcct atgatattaa gaaattactg tgctttcttt ctgctataaa agccggtatt ggtaatgtac gtgccccact ttattcgcat atgatttatg atgttttaga aaatcaaaca gttaccattg atcgtcccga tatcttaatt gttgaaggga ttaacgtgtt acaagtcaat gacctcccta aagacggtaa aattattcct tttgtttcag atttttttga tttttcaatc tatgtagatg ctgaaacaga agtcattcga cactggtatt tggaacgttt taaacgtttg cgtaaaacag cttttcaaaa tcctaagtcc tattttcatc gttatgcact catgcatgaa gaagagtcct taaaaatcgc tgaaaatatc tggcaaacaa ttaatttgaa aaatttacaa gaaaatatat cgccaacacg accacggtct aatctcattc tacgcaaggg aaaaaaacat ttggttgaat atgtggcact tcgacgacta taagtgagta aga
Expected Protein Sequence
MAHHHHHHMI DTILPKINQE DNLVKSISDR YSPYRVFTAQ EWSQFRADTP LTLTFDEIKR LRSIDDPIDL EEVQRIYLSL SRLLSCHVEA VQELFHKRQQ FLYQEGTRKT PFIIGIAGSV AVGKSTTARI LQELLKRWTS SLKVDLITTD GFLYPNAVLQ QKNRMNRKGF PDSYDIKKLL CFLSAIKAGI GNVRAPLYSH MIYDVLENQT VTIDRPDILI VEGINVLQVN DLPKDGKIIP FVSDFFDFSI YVDAETEVIR HWYLERFKRL RKTAFQNPKS YFHRYALMHE EESLKIAENI WQTINLKNLQ ENILPTRPRS NLILRKGKKH LVEYVALRRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg atacaattct cccaaaaatt aatcaggaag ataatttagt caaatcgatt tccgatcgat attcgcccta tcgtgtcttt actgcacaag aatggtctca atttcgtgca gatacaccgc ttactttgac ctttgatgaa atcaaacgtt tacgctcaat tgatgatcca attgatcttg aagaagtgca acgtatttat ctttctttat cacgtttgtt atcatgccat gttgaagctg tacaagaact ttttcacaag cgtcaacaat ttttatatca agaaggaacg agaaaaactc cttttatcat tggaattgct ggctctgttg cagtaggaaa atcaactacc gctcgtattc tacaagaact tttgaaacgt tggacttcca gtcttaaagt tgatttgatt acaacggatg gttttctcta tcctaacgct gttttacagc aaaaaaatcg tatgaatcgt aaaggatttc cagattccta tgatattaag aaattactgt gctttctttc tgctataaaa gccggtattg gtaatgtacg tgccccactt tattcgcata tgatttatga tgttttagaa aatcaaacag ttaccattga tcgtcccgat atcttaattg ttgaagggat taacgtgtta caagtcaatg acctccctaa agacggtaaa attattcctt ttgtttcaga tttttttgat ttttcaatct atgtagatgc tgaaacagaa gtcattcgac actggtattt ggaacgtttt aaacgtttgc gtaaaacagc ttttcaaaat cctaagtcct attttcatcg ttatgcactc atgcatgaag aagagtcctt aaaaatcgct gaaaatatct ggcaaacaat taatttgaaa aatttacaag aaaatatatt gccaacacga ccacggtcta atctcattct acgcaaggga aaaaaacatt tggttgaata tgtggcactt cgacgactat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaheA.00996.a.B1.PW26915
PURIFICATION DATe: 8/4/2009
CONCENTRATION: 4.84mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI DTILPKINQE DNLVKSISDR YSPYRVFTAQ EWSQFRADTP LTLTFDEIKR LRSIDDPIDL EEVQRIYLSL SRLLSCHVEA VQELFHKRQQ FLYQEGTRKT PFIIGIAGSV AVGKSTTARI LQELLKRWTS SLKVDLITTD GFLYPNAVLQ QKNRMNRKGF PDSYDIKKLL CFLSAIKAGI GNVRAPLYSH MIYDVLENQT VTIDRPDILI VEGINVLQVN DLPKDGKIIP FVSDFFDFSI YVDAETEVIR HWYLERFKRL RKTAFQNPKS YFHRYALMHE EESLKIAENI WQTINLKNLQ ENISPTRPRS NLILRKGKKH LVEYVALRRL
Validated NT Sequence
atggctcacc accaccacca ccatatgatt gatacaattc tcccaaaaat taatcaggaa gataatttag tcaaatcgat ttccgatcga tattcgccct atcgtgtctt tactgcacaa gaatggtctc aatttcgtgc agatacaccg cttactttga cctttgatga aatcaaacgt ttacgctcaa ttgatgatcc aattgatctt gaagaagtgc aacgtattta tctttcttta tcacgtttgt tatcatgcca tgttgaagct gtacaagaac tttttcacaa gcgtcaacaa tttttatatc aagaaggaac gagaaaaact ccttttatca ttggaattgc tggctctgtt gcagtaggaa aatcaactac cgctcgtatt ctacaagaac ttttgaaacg ttggacttcc agtcttaaag ttgatttgat tacaacggat ggttttctct atcctaacgc tgttttacag caaaaaaatc gtatgaatcg taaaggattt ccagattcct atgatattaa gaaattactg tgctttcttt ctgctataaa agccggtatt ggtaatgtac gtgccccact ttattcgcat atgatttatg atgttttaga aaatcaaaca gttaccattg atcgtcccga tatcttaatt gttgaaggga ttaacgtgtt acaagtcaat gacctcccta aagacggtaa aattattcct tttgtttcag atttttttga tttttcaatc tatgtagatg ctgaaacaga agtcattcga cactggtatt tggaacgttt taaacgtttg cgtaaaacag cttttcaaaa tcctaagtcc tattttcatc gttatgcact catgcatgaa gaagagtcct taaaaatcgc tgaaaatatc tggcaaacaa ttaatttgaa aaatttacaa gaaaatatat cgccaacacg accacggtct aatctcattc tacgcaaggg aaaaaaacat ttggttgaat atgtggcact tcgacgacta taagtgagta aga
Expressed Protein Sequence
MAHHHHHHMI DTILPKINQE DNLVKSISDR YSPYRVFTAQ EWSQFRADTP LTLTFDEIKR LRSIDDPIDL EEVQRIYLSL SRLLSCHVEA VQELFHKRQQ FLYQEGTRKT PFIIGIAGSV AVGKSTTARI LQELLKRWTS SLKVDLITTD GFLYPNAVLQ QKNRMNRKGF PDSYDIKKLL CFLSAIKAGI GNVRAPLYSH MIYDVLENQT VTIDRPDILI VEGINVLQVN DLPKDGKIIP FVSDFFDFSI YVDAETEVIR HWYLERFKRL RKTAFQNPKS YFHRYALMHE EESLKIAENI WQTINLKNLQ ENILPTRPRS NLILRKGKKH LVEYVALRRL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTG ATACAATTCT CCCAAAAATT AATCAGGAAG ATAATTTAGT CAAATCGATT TCCGATCGAT ATTCGCCCTA TCGTGTCTTT ACTGCACAAG AATGGTCTCA ATTTCGTGCA GATACACCGC TTACTTTGAC CTTTGATGAA ATCAAACGTT TACGCTCAAT TGATGATCCA ATTGATCTTG AAGAAGTGCA ACGTATTTAT CTTTCTTTAT CACGTTTGTT ATCATGCCAT GTTGAAGCTG TACAAGAACT TTTTCACAAG CGTCAACAAT TTTTATATCA AGAAGGAACG AGAAAAACTC CTTTTATCAT TGGAATTGCT GGCTCTGTTG CAGTAGGAAA ATCAACTACC GCTCGTATTC TACAAGAACT TTTGAAACGT TGGACTTCCA GTCTTAAAGT TGATTTGATT ACAACGGATG GTTTTCTCTA TCCTAACGCT GTTTTACAGC AAAAAAATCG TATGAATCGT AAAGGATTTC CAGATTCCTA TGATATTAAG AAATTACTGT GCTTTCTTTC TGCTATAAAA GCCGGTATTG GTAATGTACG TGCCCCACTT TATTCGCATA TGATTTATGA TGTTTTAGAA AATCAAACAG TTACCATTGA TCGTCCCGAT ATCTTAATTG TTGAAGGGAT TAACGTGTTA CAAGTCAATG ACCTCCCTAA AGACGGTAAA ATTATTCCTT TTGTTTCAGA TTTTTTTGAT TTTTCAATCT ATGTAGATGC TGAAACAGAA GTCATTCGAC ACTGGTATTT GGAACGTTTT AAACGTTTGC GTAAAACAGC TTTTCAAAAT CCTAAGTCCT ATTTTCATCG TTATGCACTC ATGCATGAAG AAGAGTCCTT AAAAATCGCT GAAAATATCT GGCAAACAAT TAATTTGAAA AATTTACAAG AAAATATATT GCCAACACGA CCACGGTCTA ATCTCATTCT ACGCAAGGGA AAAAAACATT TGGTTGAATA TGTGGCACTT CGACGACTAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT