BaheA.00993.a

acetylglutamate kinase

CENTER ID:	BaheA.00993.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00993.a.A1.GE25991	Full length( BaheA.00993.a )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130618
PATRIC ID:	fig\|283166.5.peg.82
RefSeq:	YP_032932.1
UniProt:	Q6G552

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDDVENGVAD VFEKQAAFLS SALPYMQKYE NETVVVKYGG HAMGDPALGR AFARDIALLK QSGINPVVVH GGGPQIAEIL MKMGIESRFE NGLRVTDERI VEVVEMVLAG SINKEIVALI NAEGEWAIGL CGKDGNMVFA EKAYKTVIDP DSHIERVLDL GFVGEPVEVD RTLLDLLACS EMIPVLAPVA PGRDGKTYNI NADIFAGAIA GALEAKRLLF LTDVPGVLDK KGKLLKELTI SEAENLIKNE TISGGMIPKV ETCMKALQNG VEAVVILNGR TPHSVLLELF TEQGAGTLIV S

NT Sequence

ATGGATGATG TTGAAAATGG TGTTGCAGAT GTTTTTGAAA AACAAGCAGC ATTTTTGTCG TCTGCTTTAC CTTATATGCA AAAATATGAA AATGAAACGG TCGTTGTAAA ATATGGCGGT CATGCTATGG GTGATCCTGC TTTGGGACGG GCGTTTGCAC GTGATATTGC TCTGTTGAAG CAATCAGGTA TTAACCCTGT TGTAGTCCAT GGTGGAGGTC CTCAAATTGC TGAAATTTTG ATGAAAATGG GGATTGAGTC GCGGTTTGAA AACGGTTTAC GCGTAACCGA TGAACGGATC GTGGAAGTAG TTGAAATGGT TTTAGCGGGT TCCATAAACA AAGAAATAGT CGCTCTCATA AACGCTGAAG GTGAATGGGC AATAGGATTG TGTGGCAAGG ATGGCAATAT GGTTTTTGCC GAAAAAGCTT ATAAAACTGT GATTGATCCT GATTCGCATA TTGAGCGTGT TTTGGATTTG GGATTTGTCG GTGAACCTGT TGAAGTTGAT CGCACATTAT TGGATCTTTT GGCATGTTCT GAAATGATTC CAGTTCTTGC TCCTGTAGCT CCAGGTCGCG ATGGCAAAAC CTATAATATT AATGCTGATA TTTTTGCTGG GGCCATTGCC GGTGCCTTAG AGGCTAAACG CCTTTTGTTT TTGACTGATG TTCCTGGTGT ATTGGATAAA AAGGGTAAAC TTTTAAAGGA ATTGACAATC TCTGAAGCTG AAAACCTCAT CAAAAATGAA ACAATTTCAG GTGGTATGAT TCCAAAAGTA GAAACTTGTA TGAAGGCTCT TCAAAATGGG GTGGAAGCTG TTGTCATTTT AAATGGCAGA ACTCCCCATT CTGTCTTACT AGAACTGTTT ACTGAACAGG GAGCTGGAAC GCTTATCGTT TCG

Details for BaheA.00993.a.A1.GE25991

HARVESTED ON:	1/9/2009
SEQUENCED ON:	7/28/2010
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDDVENGVA DVFEKQAAFL SSALPYMQKY ENETVVVKYG GHAMGDPALG RAFARDIALL KQSGINPVVV HGGGPQIAEI LMKMGIESRF ENGLRVTDER IVEVVEMVLA GSINKEIVAL INAEGEWAIG LCGKDGNMVF AEKAYKTVID PDSHIERVLD LGFVGEPVEV DRTLLDLLAC SEMIPVLAPV APGRDGKTYN INADIFAGAI AGALEAKRLL FLTDVPGVLD KKGKLLKELT ISEAENLIKN ETISGGMIPK VETCMKALQN GVEAVVILNG RTPHSVLLEL FTEQGAGTLI VS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatg atgttgaaaa tggtgttgca gatgtttttg aaaaacaagc agcatttttg tcgtctgctt taccttatat gcaaaaatat gaaaatgaaa cggtcgttgt aaaatatggc ggtcatgcta tgggtgatcc tgctttggga cgggcgtttg cacgtgatat tgctctgttg aagcaatcag gtattaaccc tgttgtagtc catggtggag gtcctcaaat tgctgaaatt ttgatgaaaa tggggattga gtcgcggttt gaaaacggtt tacgcgtaac cgatgaacgg atcgtggaag tagttgaaat ggttttagcg ggttccataa acaaagaaat agtcgctctc ataaacgctg aaggtgaatg ggcaatagga ttgtgtggca aggatggcaa tatggttttt gccgaaaaag cttataaaac tgtgattgat cctgattcgc atattgagcg tgttttggat ttgggatttg tcggtgaacc tgttgaagtt gatcgcacat tattggatct tttggcatgt tctgaaatga ttccagttct tgctcctgta gctccaggtc gcgatggcaa aacctataat attaatgctg atatttttgc tggggccatt gccggtgcct tagaggctaa acgccttttg tttttgactg atgttcctgg tgtattggat aaaaagggta aacttttaaa ggaattgaca atctctgaag ctgaaaacct catcaaaaat gaaacaattt caggtggtat gattccaaaa gtagaaactt gtatgaaggc tcttcaaaat ggggtggaag ctgttgtcat tttaaatggc agaactcccc attctgtctt actagaactg tttactgaac agggagctgg aacgcttatc gtttcgtaat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatgatgtt gaaaatggtg ttgcagatgt ttttgaaaaa caagcagcat ttttgtcgtc tgctttacct tatatgcaaa aatatgaaaa tgaaacggtc gttgtaaaat atggcggtca tgctatgggt gatcctgctt tgggacgggc gtttgcacgt gatattgctc tgttgaagca atcaggtatt aaccctgttg tagtccatgg tggaggtcct caaattgctg aaattttgat gaaaatgggg attgagtcgc ggtttgaaaa cggtttacgc gtaaccgatg aacggatcgt ggaagtagtt gaaatggttt tagcgggttc cataaacaaa gaaatagtcg ctctcataaa cgctgaaggt gaatgggcaa taggattgtg tggcaaggat ggcaatatgg tttttgccga aaaagcttat aaaactgtga ttgatcctga ttcgcatatt gagcgtgttt tggatttggg atttgtcggt gaacctgttg aagttgatcg cacattattg gatcttttgg catgttctga aatgattcca gttcttgctc ctgtagctcc aggtcgcgat ggcaaaacct ataatattaa tgctgatatt tttgctgggg ccattgccgg tgccttagag gctaaacgcc ttttgttttt gactgatgtt cctggtgtat tggataaaaa gggtaaactt ttaaaggaat tgacaatctc tgaagctgaa aacctcatca aaaatgaaac aatttcaggt ggtatgattc caaaagtaga aacttgtatg aaggctcttc aaaatggggt ggaagctgtt gtcattttaa atggcagaac tccccattct gtcttactag aactgtttac tgaacaggga gctggaacgc ttatcgtttc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa