BaheA.00944.a
ribose-5-phosphate isomerase A
| CENTER ID: | BaheA.00944.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00944.a.A1.GE25979 | Full length( BaheA.00944.a ) | 1 | 234 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3HHE
DEPOSITED: 5/15/2009
DETERMINATION: XRay
CLONE: BaheA.00944.a.A1.GE25979
PROTEIN: BaheA.00944.a.A1.PW26001
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127414 |
| PATRIC ID: | fig|283166.5.peg.698 |
| RefSeq: | YP_033470.1 |
| UniProt: | Q6G3V6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVQQLKKMA ALKALEFVED DMRLGIGSGS TVNEFIPLLG ERVANGLRVT CVATSQYSEQ LCHKFGVPIS TLEKIPELDL DIDGADEIGP EMTLIKGGGG ALLHEKIVAS ASRAMFVIAD ETKMVKTLGA FALPIEVNPF GIHATRIAIE KAADNLGLSG EITLRMNGDD PFKTDGGHFI FDAFWGRILQ PKLLSEALLA IPGVVEHGLF LGLASRAIVA MADSQIKVLE PFDF
NT Sequence
ATGAATGTTC AGCAATTAAA AAAAATGGCG GCTTTAAAAG CACTTGAATT TGTTGAAGAT GATATGCGGC TTGGTATCGG ATCTGGTTCG ACAGTAAATG AGTTTATCCC TCTTCTTGGT GAGCGTGTTG CGAATGGTCT GCGCGTGACT TGTGTTGCAA CCTCGCAGTA TTCTGAACAA CTCTGTCATA AATTTGGCGT GCCTATCAGC ACCTTAGAAA AAATACCTGA ACTAGACCTT GATATTGATG GGGCGGATGA AATTGGTCCA GAGATGACTC TTATTAAAGG AGGGGGTGGA GCATTGTTGC ATGAAAAAAT TGTAGCATCG GCTTCTCGTG CAATGTTTGT CATCGCGGAT GAAACAAAAA TGGTAAAAAC GCTTGGTGCT TTTGCATTGC CGATTGAAGT TAATCCGTTT GGTATACATG CTACACGCAT CGCCATTGAA AAAGCAGCTG ATAATTTAGG ACTTTCGGGG GAAATTACAT TACGAATGAA TGGCGACGAT CCTTTTAAAA CAGATGGTGG TCATTTCATT TTTGATGCAT TTTGGGGACG CATTTTACAG CCAAAATTAT TATCGGAGGC ACTTCTTGCA ATTCCTGGTG TTGTTGAGCA TGGTCTTTTT TTGGGATTGG CTTCACGTGC AATTGTAGCG ATGGCGGATA GTCAGATAAA AGTTTTAGAA CCGTTTGATT TT
Details for BaheA.00944.a.A1.GE25979
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNVQQLKKM AALKALEFVE DDMRLGIGSG STVNEFIPLL GERVANGLRV TCVATSQYSE QLCHKFGVPI STLEKIPELD LDIDGADEIG PEMTLIKGGG GALLHEKIVA SASRAMFVIA DETKMVKTLG AFALPIEVNP FGIHATRIAI EKAADNLGLS GKITLRMNGD DPFKTDGGHF IFDAFWGRIL QPKLLSEALL AIPGVVEHGL FLGLASRAIV AMADSQIKVL EPF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatg ttcagcaatt aaaaaaaatg gcggctttaa aagcacttga atttgttgaa gatgatatgc ggcttggtat cggatctggt tcgacagtaa atgagtttat ccctcttctt ggtgagcgtg ttgcgaatgg tctgcgcgtg acttgtgttg caacctcgca gtattctgaa caactctgtc ataaatttgg cgtgcctatc agcaccttag aaaaaatacc tgaactagac cttgatattg atggggcgga tgaaattggt ccagagatga ctcttattaa aggagggggt ggagcattgt tgcatgaaaa aattgtagca tcggcttctc gtgcaatgtt tgtcatcgcg gatgaaacaa aaatggtaaa aacgcttggt gcttttgcat tgccgattga agttaatccg tttggtatac atgctacacg catcgccatt gaaaaagcag ctgataattt aggactttcg gggaaaatta cattacgaat gaatggcgac gatcctttta aaacagatgg tggtcatttc atttttgatg cattttgggg acgcatttta cagccaaaat tattatcgga ggcacttctt gcaattcctg gtgttgttga gcatggtctt tttttgggat tggcttcacg tgcaattgta gcgatggcgg atagtcagat aaaagtttta gaaccgtttg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNVQQLKKM AALKALEFVE DDMRLGIGSG STVNEFIPLL GERVANGLRV TCVATSQYSE QLCHKFGVPI STLEKIPELD LDIDGADEIG PEMTLIKGGG GALLHEKIVA SASRAMFVIA DETKMVKTLG AFALPIEVNP FGIHATRIAI EKAADNLGLS GEITLRMNGD DPFKTDGGHF IFDAFWGRIL QPKLLSEALL AIPGVVEHGL FLGLASRAIV AMADSQIKVL EPFDF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgttcag caattaaaaa aaatggcggc tttaaaagca cttgaatttg ttgaagatga tatgcggctt ggtatcggat ctggttcgac agtaaatgag tttatccctc ttcttggtga gcgtgttgcg aatggtctgc gcgtgacttg tgttgcaacc tcgcagtatt ctgaacaact ctgtcataaa tttggcgtgc ctatcagcac cttagaaaaa atacctgaac tagaccttga tattgatggg gcggatgaaa ttggtccaga gatgactctt attaaaggag ggggtggagc attgttgcat gaaaaaattg tagcatcggc ttctcgtgca atgtttgtca tcgcggatga aacaaaaatg gtaaaaacgc ttggtgcttt tgcattgccg attgaagtta atccgtttgg tatacatgct acacgcatcg ccattgaaaa agcagctgat aatttaggac tttcggggga aattacatta cgaatgaatg gcgacgatcc ttttaaaaca gatggtggtc atttcatttt tgatgcattt tggggacgca ttttacagcc aaaattatta tcggaggcac ttcttgcaat tcctggtgtt gttgagcatg gtcttttttt gggattggct tcacgtgcaa ttgtagcgat ggcggatagt cagataaaag ttttagaacc gtttgatttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa