BaheA.00888.a

adenylosuccinate synthetase

CENTER ID: BaheA.00888.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00888.a.B1.GE43956 Full length( BaheA.00888.a ) 1 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.410
RefSeq: YP_033220.1
UniProt: Q6G4G5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANVVVVGTQ WGDEGKGKIV DWLSERADII VRYQGGHNAG HTLVVNGVNY KLSLLPSGLV RGKLSIIGNG VVVDPHHFVV ELKKLRDQGV KIVPEILRIA ENAPLILSLH RDLDAIRESG FSGLKIGTTK RGIGPAYEDK VGRRAIRVMD LAEANTLMAK IKRLLRHHNA LRRGMGVAEI DSQALYDELM QVADEILPFM DCTWRLLDES YRMGKHVLFE GAQGALLDND FGTYPYVTSS NTVAGQACTG SGMGPGAIHY VLGIAKAYTT RVGEGPFPTE QINDIGEFLG IRGHEFGVVT GRKRRCGWFD AVLVRQMVTI CGVQGIALTK LDVLDGLDEI KVCVGYELDG KRIDYLPSSM GAQSRVKPIY ETLEGWKQAT AHALKWEDLP VQAIKYIRYI EELIGTQVAL LSTSPEREDT ILITDPFAN
NT Sequence
ATGGCCAATG TAGTAGTTGT AGGGACGCAA TGGGGCGATG AAGGCAAAGG TAAAATTGTA GATTGGTTGT CGGAGCGGGC AGATATCATA GTTAGATATC AGGGTGGACA CAATGCAGGC CATACATTGG TTGTTAATGG AGTTAATTAT AAATTATCAC TTTTACCGTC TGGTTTAGTT CGTGGAAAAT TATCAATTAT CGGTAATGGT GTTGTCGTTG ACCCTCATCA TTTTGTAGTA GAATTAAAAA AACTACGTGA TCAAGGTGTG AAAATTGTAC CGGAAATTTT ACGTATTGCT GAAAATGCTC CATTGATTCT TTCCTTACAT CGCGATCTTG ACGCAATTCG CGAAAGTGGT TTTTCGGGTT TAAAAATTGG TACGACAAAG CGTGGTATTG GTCCGGCTTA TGAAGATAAG GTGGGGCGTC GCGCTATCCG TGTGATGGAT TTGGCGGAAG CTAATACGCT TATGGCTAAG ATTAAACGAC TTTTGCGACA TCATAATGCT TTGCGTCGTG GGATGGGCGT TGCAGAGATT GATTCTCAAG CGCTTTATGA TGAATTAATG CAAGTGGCGG ATGAAATTTT ACCCTTTATG GATTGTACTT GGCGTCTTTT GGATGAAAGT TATCGGATGG GTAAGCACGT TCTTTTTGAA GGTGCACAAG GTGCTTTATT AGATAATGAT TTCGGGACTT ATCCTTATGT GACGTCATCC AATACAGTTG CTGGGCAGGC GTGTACTGGC TCAGGTATGG GACCTGGTGC AATTCATTAT GTTTTGGGTA TTGCAAAAGC TTATACAACA CGTGTTGGAG AAGGGCCGTT TCCGACAGAG CAGATAAATG ATATTGGCGA ATTTCTTGGA ATACGTGGGC ATGAATTTGG CGTTGTTACT GGTCGAAAAC GTCGATGTGG CTGGTTTGAC GCTGTCTTAG TCCGTCAAAT GGTAACGATT TGTGGTGTTC AGGGTATAGC ATTGACAAAG CTTGATGTTT TGGATGGTTT GGATGAAATT AAAGTCTGTG TTGGTTATGA ACTTGATGGG AAAAGAATTG ATTATTTACC CTCTTCCATG GGAGCACAAT CCCGTGTAAA GCCTATTTAT GAGACATTAG AAGGTTGGAA GCAAGCAACA GCGCATGCGT TGAAGTGGGA AGATTTACCA GTACAAGCTA TCAAATATAT ACGCTATATT GAAGAATTGA TCGGTACGCA GGTGGCTTTA TTATCCACGA GCCCTGAGCG TGAAGATACT ATCCTTATTA CGGATCCTTT TGCAAAT
Details for BaheA.00888.a.B1.GE43956
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA NVVVVGTQWG DEGKGKIVXW LSERADIIVR YQGGHNAGHT LVVNGVNYKL SLLPSGLVRG KLSIIGNGVV VDPHHFVVEL KKLRDQGVKI VPEILRIAEN APLILSLHRD LDAIRESGFS GLKIGTTKRG IGPAYEDKVG RRAIRVMDLA EANTLMAKIK RLLRHHNALR RGMGVAEIDS QALYDELMQV ADEILPFMDC TWRLLDESYR MGKHVLFEGA QGALLDNDFG TYPYVTSSNT VAGQACTGSG MGPGAIHYVL GIAKAYTTRV GEGPFPTEQI NDIGEFLGIR GHEFGVVTGR KRRCGWFDAV LVRQMVTICG VQGIALTKLD VLDGLDEIKV CVGYELDGKR IDYLPSSMGA QSRVKPIYET LEGWKQATAH ALKWEDLPVQ AIKYIRYIEE LIGTQVALLS TSPEREDTIL
Validated NT Sequence
atggctcacc accaccacca ccatatggcc aatgtagtag ttgtagggac gcaatggggc gatgaaggca aaggtaaaat tgtanattgg ttgtcggagc gggcagatat catagttaga tatcagggtg gacacaatgc aggccataca ttggttgtta atggagttaa ttataaatta tcacttttac cgtctggttt agttcgtgga aaattatcaa ttatcggtaa tggtgttgtc gttgaccctc atcattttgt agtagaatta aaaaaactac gtgatcaagg tgtgaaaatt gtaccggaaa ttttacgtat tgctgaaaat gctccattga ttctttcctt acatcgcgat cttgacgcaa ttcgcgaaag tggtttttcg ggtttaaaaa ttggtacgac aaagcgtggt attggtccgg cttatgaaga taaggtgggg cgtcgcgcta tccgtgtgat ggatttggcg gaagctaata cgcttatggc taagattaaa cgacttttgc gacatcataa tgctttgcgt cgtgggatgg gcgttgcaga gattgattct caagcgcttt atgatgaatt aatgcaagtg gcggatgaaa ttttaccctt tatggattgt acttggcgtc ttttggatga aagttatcgg atgggtaagc acgttctttt tgaaggtgca caaggtgctt tattagataa tgatttcggg acttatcctt atgtgacgtc atccaataca gttgctgggc aggcgtgtac tggctcaggt atgggacctg gtgcaattca ttatgttttg ggtattgcaa aagcttatac aacacgtgtt ggagaagggc cgtttccgac agagcagata aatgatattg gcgaatttct tggaatacgt gggcatgaat ttggcgttgt tactggtcga aaacgtcgat gtggctggtt tgacgctgtc ttagtccgtc aaatggtaac gatttgtggt gttcagggta tagcattgac aaagcttgat gttttggatg gtttggatga aattaaagtc tgtgttggtt atgaacttga tgggaaaaga attgattatt taccctcttc catgggagca caatcccgtg taaagcctat ttatgagaca ttagaaggtt ggaagcaagc aacagcgcat gcgttgaagt gggaagattt accagtacaa gctatcaaat atatacgcta tattgaagaa ttgatcggta cgcaggtggc tttattatcc acgagccctg agcgtgaaga tactatcctt att
Expected Protein Sequence
MAHHHHHHMA NVVVVGTQWG DEGKGKIVDW LSERADIIVR YQGGHNAGHT LVVNGVNYKL SLLPSGLVRG KLSIIGNGVV VDPHHFVVEL KKLRDQGVKI VPEILRIAEN APLILSLHRD LDAIRESGFS GLKIGTTKRG IGPAYEDKVG RRAIRVMDLA EANTLMAKIK RLLRHHNALR RGMGVAEIDS QALYDELMQV ADEILPFMDC TWRLLDESYR MGKHVLFEGA QGALLDNDFG TYPYVTSSNT VAGQACTGSG MGPGAIHYVL GIAKAYTTRV GEGPFPTEQI NDIGEFLGIR GHEFGVVTGR KRRCGWFDAV LVRQMVTICG VQGIALTKLD VLDGLDEIKV CVGYELDGKR IDYLPSSMGA QSRVKPIYET LEGWKQATAH ALKWEDLPVQ AIKYIRYIEE LIGTQVALLS TSPEREDTIL ITDPFAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca atgtagtagt tgtagggacg caatggggcg atgaaggcaa aggtaaaatt gtagattggt tgtcggagcg ggcagatatc atagttagat atcagggtgg acacaatgca ggccatacat tggttgttaa tggagttaat tataaattat cacttttacc gtctggttta gttcgtggaa aattatcaat tatcggtaat ggtgttgtcg ttgaccctca tcattttgta gtagaattaa aaaaactacg tgatcaaggt gtgaaaattg taccggaaat tttacgtatt gctgaaaatg ctccattgat tctttcctta catcgcgatc ttgacgcaat tcgcgaaagt ggtttttcgg gtttaaaaat tggtacgaca aagcgtggta ttggtccggc ttatgaagat aaggtggggc gtcgcgctat ccgtgtgatg gatttggcgg aagctaatac gcttatggct aagattaaac gacttttgcg acatcataat gctttgcgtc gtgggatggg cgttgcagag attgattctc aagcgcttta tgatgaatta atgcaagtgg cggatgaaat tttacccttt atggattgta cttggcgtct tttggatgaa agttatcgga tgggtaagca cgttcttttt gaaggtgcac aaggtgcttt attagataat gatttcggga cttatcctta tgtgacgtca tccaatacag ttgctgggca ggcgtgtact ggctcaggta tgggacctgg tgcaattcat tatgttttgg gtattgcaaa agcttataca acacgtgttg gagaagggcc gtttccgaca gagcagataa atgatattgg cgaatttctt ggaatacgtg ggcatgaatt tggcgttgtt actggtcgaa aacgtcgatg tggctggttt gacgctgtct tagtccgtca aatggtaacg atttgtggtg ttcagggtat agcattgaca aagcttgatg ttttggatgg tttggatgaa attaaagtct gtgttggtta tgaacttgat gggaaaagaa ttgattattt accctcttcc atgggagcac aatcccgtgt aaagcctatt tatgagacat tagaaggttg gaagcaagca acagcgcatg cgttgaagtg ggaagattta ccagtacaag ctatcaaata tatacgctat attgaagaat tgatcggtac gcaggtggct ttattatcca cgagccctga gcgtgaagat actatcctta ttacggatcc ttttgcaaat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat