SSGCID: Target Page

BaheA.00867.a

Phosphomethylpyrimidine kinase

CENTER ID:	BaheA.00867.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00867.a.B1.GE27112	Full length( BaheA.00867.a )		1	252

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.527
RefSeq:	YP_033322.1
UniProt:	A0A0H3M558

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALMPSILIV AGTDPTGGAG IVRDIETAAH FQIKANLAIT CINVQDDNHV AEIVPMCEKL IAAQMRAALE ANPISAIKIG MTGNQAIIAA ICNVIEDYPH IPVILDPVLF ASSGGKLTTE PIIEIMINKL LPYVDLLTPN REELALLSQT PLASCHKQAI QQAKKLLSFG PRSILIKGGH ADGPLATDSF IDKTEIIDIS TVRLTRTMRG TGCILSSAIA AHLALNESMI QAIKNAKEYI YRLLLNHNQK GD

NT Sequence

ATGGCGTTGA TGCCTTCTAT TCTTATTGTT GCAGGCACCG ATCCAACAGG AGGTGCTGGT ATTGTTCGTG ATATAGAAAC AGCTGCACAT TTTCAAATAA AAGCAAATTT AGCAATTACT TGCATTAACG TACAAGATGA TAACCATGTT GCTGAAATTG TTCCAATGTG CGAAAAACTC ATAGCTGCGC AAATGCGCGC AGCCTTAGAG GCCAATCCAA TAAGCGCAAT AAAAATTGGG ATGACAGGAA ATCAAGCAAT TATCGCAGCA ATCTGTAATG TCATAGAAGA CTATCCTCAT ATTCCGGTTA TTCTTGACCC GGTACTTTTT GCCTCATCAG GAGGAAAACT AACAACGGAA CCAATCATAG AAATTATGAT CAATAAACTT CTCCCTTACG TTGATCTTTT AACACCCAAT AGGGAAGAGC TCGCCCTTCT CAGCCAAACC CCATTGGCAT CTTGTCATAA GCAAGCAATA CAACAAGCAA AAAAACTTCT GTCATTTGGT CCACGTTCTA TCTTGATAAA AGGTGGACAT GCAGATGGTC CCCTTGCAAC TGATAGCTTC ATTGATAAAA CTGAAATCAT CGATATTTCC ACTGTGCGCT TAACGAGAAC AATGCGTGGA ACAGGTTGCA TCCTCTCCAG CGCTATTGCA GCACATTTGG CATTAAATGA ATCTATGATA CAAGCTATAA AAAATGCAAA AGAATATATT TACAGATTAC TGTTAAATCA CAATCAAAAA GGTGAT

Details for BaheA.00867.a.B1.GE27112

HARVESTED ON:	8/17/2009
SEQUENCED ON:	9/29/2009
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMX LMPSILIVAG TDPTGGAGIV RDIETAAHFQ IKANLAITCI NVQDDNHVAE IVPMCEKLIA AQMREALEAN PISAIKIGMT GNQAIIAAIC NVIEDYPHIP VILDPVLFVS SGGKLTTEPI IEIMINKLLP YVDLLKPNRE ELALLSQTPL ASCHKQAIQQ AKKPLSFGPR SILIKGGHAD GPLATDSFID KTEIIDIXTV RLTRTMRGTG CILSSAIAAH LALNEXMIQA IKNAKEYIYR LLLNHNQKGI K

Validated NT Sequence

anaccatggc tcaccaccac caccaccata tggngttgat gccttctatt cttattgttg caggcaccga tccaacagga ggtgctggta ttgttcgtga tatagaaaca gctgcacatt ttcaaataaa agcaaattta gcaattactt gcattaacgt acaagatgat aaccatgttg ctgaaattgt tccaatgtgc gaaaaactca tagctgcgca aatgcgcgaa gccttagagg ccaatccaat aagcgcaata aaaattggga tgacaggaaa tcaagcaatt atcgcagcaa tctgtaatgt catagaagac tatcctcata ttccggttat tcttgacccg gtactttttg tctcatcagg aggaaaacta acaacggaac caatcataga aattatgatc aataaacttc tcccttacgt tgatctttta aaacccaata gggaagagct cgcccttctc agccaaaccc cattggcatc ttgtcataag caagcaatac aacaagcaaa aaaacctctg tcatttggtc cacgttctat cttgataaaa ggtggacatg cagatggtcc ccttgcaact gatagcttca ttgataaaac tgaaatcatc gatatttnca ctgtgcgctt aacgagaaca atgcgtggaa caggttgcat cctctccagc gctattgcag cacatttggc attaaatgaa tntatgatac aagctataaa aaatgcaaaa gaatatattt acagattact gttaaatcac aatcaaaaag gtattaagtg agtaagatag g

Expected Protein Sequence

MAHHHHHHMA LMPSILIVAG TDPTGGAGIV RDIETAAHFQ IKANLAITCI NVQDDNHVAE IVPMCEKLIA AQMRAALEAN PISAIKIGMT GNQAIIAAIC NVIEDYPHIP VILDPVLFAS SGGKLTTEPI IEIMINKLLP YVDLLTPNRE ELALLSQTPL ASCHKQAIQQ AKKLLSFGPR SILIKGGHAD GPLATDSFID KTEIIDISTV RLTRTMRGTG CILSSAIAAH LALNESMIQA IKNAKEYIYR LLLNHNQKGD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgt tgatgccttc tattcttatt gttgcaggca ccgatccaac aggaggtgct ggtattgttc gtgatataga aacagctgca cattttcaaa taaaagcaaa tttagcaatt acttgcatta acgtacaaga tgataaccat gttgctgaaa ttgttccaat gtgcgaaaaa ctcatagctg cgcaaatgcg cgcagcctta gaggccaatc caataagcgc aataaaaatt gggatgacag gaaatcaagc aattatcgca gcaatctgta atgtcataga agactatcct catattccgg ttattcttga cccggtactt tttgcctcat caggaggaaa actaacaacg gaaccaatca tagaaattat gatcaataaa cttctccctt acgttgatct tttaacaccc aatagggaag agctcgccct tctcagccaa accccattgg catcttgtca taagcaagca atacaacaag caaaaaaact tctgtcattt ggtccacgtt ctatcttgat aaaaggtgga catgcagatg gtccccttgc aactgatagc ttcattgata aaactgaaat catcgatatt tccactgtgc gcttaacgag aacaatgcgt ggaacaggtt gcatcctctc cagcgctatt gcagcacatt tggcattaaa tgaatctatg atacaagcta taaaaaatgc aaaagaatat atttacagat tactgttaaa tcacaatcaa aaaggtgatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat