BaheA.00852.a

GTP-binding protein EngA

CENTER ID: BaheA.00852.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00852.a.A1.GE25891 Full length( BaheA.00852.a ) 1 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00852.a.A1.PS00148 Full length( BaheA.00852.a ) 1 475

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.443
RefSeq: YP_033248.1
UniProt: Q6G5J7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLTIAIVGR PNVGKSTLFN RLVGQKLALV DDKPGVTRDR RVHAATLQDL RFDVIDTAGL EETGDHTLEG RMRAHTKAAI DEADLILFVF DAKSGITPSD LNFASLVRKS GKPIVLVANK SESKAAIGGE YEAWSLGLGE PCSISAEHGL GLSDLRDAIM KASGGERIFA SKNQEEHIAL QSASLDDYID NLEEKGDVYD KSKPLRIAVA GRPNTGKSTL INRMLGQDRL LTGPEAGLTR DSISVDWEWR GRHIKLFDTA GLRRKSKIQE KLEKLSVADT LRAIRFAEVV VIVFDATTPF EKQDLQIADL VIREGRVPLI AFNKWDLIEN SQATLVDLHE KCIRLLPQVR GLRAVPLSGQ YGQGIDKLME NVMMMHRVWN RRISTGKLNR WLETIVAHHP PPAVLGRRLK VKYVTQVKTR PPGFVFSCSR PKLMPQSYLR YLSNELRNTF DMPGIPIRIS LRASDNPFAT RSKKK
NT Sequence
ATGAGCCTTA CCATTGCTAT AGTTGGTCGT CCTAATGTTG GAAAGTCAAC GCTTTTTAAT CGTTTGGTTG GGCAAAAATT GGCTTTGGTT GATGATAAGC CTGGTGTGAC ACGCGATCGG CGTGTTCATG CTGCAACACT TCAAGATCTG CGTTTTGATG TGATTGATAC GGCTGGGTTG GAAGAAACCG GTGATCATAC ACTCGAAGGT CGTATGCGTG CTCATACAAA AGCTGCTATC GATGAAGCGG ATCTTATTTT ATTTGTATTT GATGCAAAGA GTGGAATAAC GCCGAGTGAT TTAAATTTTG CGTCACTGGT TCGCAAATCA GGCAAGCCGA TTGTGCTTGT TGCCAATAAA TCTGAGTCAA AAGCGGCAAT TGGGGGAGAA TATGAGGCAT GGTCGTTAGG GTTGGGGGAG CCTTGTTCAA TATCTGCTGA ACATGGTTTA GGTCTTTCGG ATCTTCGTGA TGCAATTATG AAGGCTAGTG GGGGAGAGAG AATTTTTGCA AGCAAAAACC AAGAAGAACA TATTGCTTTA CAGTCAGCAT CTCTTGATGA TTATATTGAT AATTTAGAAG AAAAGGGGGA TGTTTACGAT AAAAGTAAAC CCCTTCGGAT TGCAGTTGCT GGTCGCCCAA ACACCGGAAA ATCAACTCTC ATCAATAGGA TGTTAGGGCA AGATCGTTTG TTGACAGGAC CTGAAGCAGG ATTAACTCGT GATTCCATTT CTGTGGATTG GGAATGGCGC GGTCGTCATA TTAAGCTTTT TGATACTGCA GGTTTACGTC GAAAGTCGAA AATTCAAGAA AAATTAGAAA AACTTTCTGT TGCAGATACT TTGCGTGCAA TTCGTTTTGC GGAAGTGGTA GTAATTGTTT TTGATGCAAC TACACCTTTT GAAAAGCAGG ATTTACAAAT TGCTGATCTT GTGATTCGTG AAGGGCGTGT TCCACTTATT GCTTTCAATA AGTGGGATCT TATTGAAAAT AGTCAGGCAA CATTGGTTGA TTTGCATGAA AAATGCATCC GCCTTCTTCC TCAAGTTCGT GGTTTGAGAG CTGTTCCTTT ATCGGGTCAA TATGGTCAAG GGATTGATAA ATTGATGGAA AATGTCATGA TGATGCACCG TGTGTGGAAT CGTCGTATTT CCACGGGAAA ATTGAACCGA TGGCTTGAAA CGATCGTTGC GCATCATCCC CCGCCTGCGG TTTTGGGGCG TCGACTTAAG GTCAAATATG TCACACAGGT TAAGACACGT CCTCCTGGAT TTGTGTTTTC TTGCTCGCGA CCAAAGCTCA TGCCTCAATC GTATTTACGT TATCTTTCTA ATGAATTGCG TAATACGTTT GATATGCCTG GGATACCAAT TCGCATATCA TTACGGGCAT CTGATAATCC CTTTGCAACG CGTTCCAAAA AGAAA
Details for BaheA.00852.a.A1.GE25891
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 48
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLTIAIVG RPNVGKSTLF NRLVGQKLAL VDDKPGVTRD RRVHAATLQD LRFDVIDTAG LEETGDHTLE GRMRAHTKAA IDEADLILFV FDAKSGITPS DLNFASLVRK SGKPIVLVAN KSESKAAIGG EYEAWSLGLG EPCSISAEHG LGLSDLRDAI MKASGGERIF ASKNQEEHIA LQSASLDDYI DXLXEKGDVY XKSKPXSDCS CWSPXXRKXN SHQ
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagcctt accattgcta tagttggtcg tcctaatgtt ggaaagtcaa cgctttttaa tcgtttggtt gggcaaaaat tggctttggt tgatgataag cctggtgtga cacgcgatcg gcgtgttcat gctgcaacac ttcaagatct gcgttttgat gtgattgata cggctgggtt ggaagaaacc ggtgatcata cactcgaagg tcgtatgcgt gctcatacaa aagctgctat cgatgaagcg gatcttattt tatttgtatt tgatgcaaag agtggaataa cgccgagtga tttaaatttt gcgtcactgg ttcgcaaatc aggcaagccg attgtgcttg ttgccaataa atctgagtca aaagcggcaa ttgggggaga atatgaggca tggtcgttag ggttggggga gccttgttca atatctgctg aacatggttt aggtctttcg gatcttcgtg atgcaattat gaaggctagt gggggagaga gaatttttgc aagcaaaaac caagaagaac atattgcttt acagtcagca tctcttgatg attatattga tantttanaa gaaaaggggg atgtttacna naaaagtaaa ccccnttcgg attgcagttg ctggtcnccc naacnccgga aantcaactc tcatcaatag gatgttaggg cnngntcntt ngnngacnng acctnaagca ggattaactc gtgattccat ttctgtngat ngggaatggc gcggtcgtca tattaagctt tttgatactg caggtttacg tcgaaagtcg aaaattcaag aaaaattaga aaaactttct gttgcagata ctttgcgtgc aattcgtttt gcggaagtgg tagtaattgt tttngatgca actacacctt ttgaaaagca ggatttacaa attgctgatc ttgtgattcg tgaagggcgt gttccactta ttgctttcaa taagtgggat cttattgaaa atagtcaggc aacattggtt gatttgcatg aaaaatgcat ccgccttctt cctcaagttc gtggtttgag agctgttcct ttatcgggtc aatatggtca agggattgat aaattgatgg aaaatgtcat gatgatgcac cgtgtgtgga atcgtcgtat ttccacggga aaattgaacc gatggcttga aacgatcgtt gcgcatcatc ccccgcctgc ggttttgggg cgtcgactta aggtcaaata tgtcacacag gttaagacac gtcctcctgg atttgtgttt tcttgctcgc gaccaaagct catgcctcaa tcgtatttac gttatctttc taatgaattg cgtaatacgt ttgatatgcc tgggatacca attcgcatat cattacgggc atctgataat ccctttgcaa cgcgttccaa aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLTIAIVG RPNVGKSTLF NRLVGQKLAL VDDKPGVTRD RRVHAATLQD LRFDVIDTAG LEETGDHTLE GRMRAHTKAA IDEADLILFV FDAKSGITPS DLNFASLVRK SGKPIVLVAN KSESKAAIGG EYEAWSLGLG EPCSISAEHG LGLSDLRDAI MKASGGERIF ASKNQEEHIA LQSASLDDYI DNLEEKGDVY DKSKPLRIAV AGRPNTGKST LINRMLGQDR LLTGPEAGLT RDSISVDWEW RGRHIKLFDT AGLRRKSKIQ EKLEKLSVAD TLRAIRFAEV VVIVFDATTP FEKQDLQIAD LVIREGRVPL IAFNKWDLIE NSQATLVDLH EKCIRLLPQV RGLRAVPLSG QYGQGIDKLM ENVMMMHRVW NRRISTGKLN RWLETIVAHH PPPAVLGRRL KVKYVTQVKT RPPGFVFSCS RPKLMPQSYL RYLSNELRNT FDMPGIPIRI SLRASDNPFA TRSKKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccttacc attgctatag ttggtcgtcc taatgttgga aagtcaacgc tttttaatcg tttggttggg caaaaattgg ctttggttga tgataagcct ggtgtgacac gcgatcggcg tgttcatgct gcaacacttc aagatctgcg ttttgatgtg attgatacgg ctgggttgga agaaaccggt gatcatacac tcgaaggtcg tatgcgtgct catacaaaag ctgctatcga tgaagcggat cttattttat ttgtatttga tgcaaagagt ggaataacgc cgagtgattt aaattttgcg tcactggttc gcaaatcagg caagccgatt gtgcttgttg ccaataaatc tgagtcaaaa gcggcaattg ggggagaata tgaggcatgg tcgttagggt tgggggagcc ttgttcaata tctgctgaac atggtttagg tctttcggat cttcgtgatg caattatgaa ggctagtggg ggagagagaa tttttgcaag caaaaaccaa gaagaacata ttgctttaca gtcagcatct cttgatgatt atattgataa tttagaagaa aagggggatg tttacgataa aagtaaaccc cttcggattg cagttgctgg tcgcccaaac accggaaaat caactctcat caataggatg ttagggcaag atcgtttgtt gacaggacct gaagcaggat taactcgtga ttccatttct gtggattggg aatggcgcgg tcgtcatatt aagctttttg atactgcagg tttacgtcga aagtcgaaaa ttcaagaaaa attagaaaaa ctttctgttg cagatacttt gcgtgcaatt cgttttgcgg aagtggtagt aattgttttt gatgcaacta caccttttga aaagcaggat ttacaaattg ctgatcttgt gattcgtgaa gggcgtgttc cacttattgc tttcaataag tgggatctta ttgaaaatag tcaggcaaca ttggttgatt tgcatgaaaa atgcatccgc cttcttcctc aagttcgtgg tttgagagct gttcctttat cgggtcaata tggtcaaggg attgataaat tgatggaaaa tgtcatgatg atgcaccgtg tgtggaatcg tcgtatttcc acgggaaaat tgaaccgatg gcttgaaacg atcgttgcgc atcatccccc gcctgcggtt ttggggcgtc gacttaaggt caaatatgtc acacaggtta agacacgtcc tcctggattt gtgttttctt gctcgcgacc aaagctcatg cctcaatcgt atttacgtta tctttctaat gaattgcgta atacgtttga tatgcctggg ataccaattc gcatatcatt acgggcatct gataatccct ttgcaacgcg ttccaaaaag aaaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BaheA.00852.a.A1.PS00148
PURIFICATION DATe: 2/19/2009
CONCENTRATION: 6.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 48
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLTIAIVG RPNVGKSTLF NRLVGQKLAL VDDKPGVTRD RRVHAATLQD LRFDVIDTAG LEETGDHTLE GRMRAHTKAA IDEADLILFV FDAKSGITPS DLNFASLVRK SGKPIVLVAN KSESKAAIGG EYEAWSLGLG EPCSISAEHG LGLSDLRDAI MKASGGERIF ASKNQEEHIA LQSASLDDYI DXLXEKGDVY XKSKPXSDCS CWSPXXRKXN SHQ
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagcctt accattgcta tagttggtcg tcctaatgtt ggaaagtcaa cgctttttaa tcgtttggtt gggcaaaaat tggctttggt tgatgataag cctggtgtga cacgcgatcg gcgtgttcat gctgcaacac ttcaagatct gcgttttgat gtgattgata cggctgggtt ggaagaaacc ggtgatcata cactcgaagg tcgtatgcgt gctcatacaa aagctgctat cgatgaagcg gatcttattt tatttgtatt tgatgcaaag agtggaataa cgccgagtga tttaaatttt gcgtcactgg ttcgcaaatc aggcaagccg attgtgcttg ttgccaataa atctgagtca aaagcggcaa ttgggggaga atatgaggca tggtcgttag ggttggggga gccttgttca atatctgctg aacatggttt aggtctttcg gatcttcgtg atgcaattat gaaggctagt gggggagaga gaatttttgc aagcaaaaac caagaagaac atattgcttt acagtcagca tctcttgatg attatattga tantttanaa gaaaaggggg atgtttacna naaaagtaaa ccccnttcgg attgcagttg ctggtcnccc naacnccgga aantcaactc tcatcaatag gatgttaggg cnngntcntt ngnngacnng acctnaagca ggattaactc gtgattccat ttctgtngat ngggaatggc gcggtcgtca tattaagctt tttgatactg caggtttacg tcgaaagtcg aaaattcaag aaaaattaga aaaactttct gttgcagata ctttgcgtgc aattcgtttt gcggaagtgg tagtaattgt tttngatgca actacacctt ttgaaaagca ggatttacaa attgctgatc ttgtgattcg tgaagggcgt gttccactta ttgctttcaa taagtgggat cttattgaaa atagtcaggc aacattggtt gatttgcatg aaaaatgcat ccgccttctt cctcaagttc gtggtttgag agctgttcct ttatcgggtc aatatggtca agggattgat aaattgatgg aaaatgtcat gatgatgcac cgtgtgtgga atcgtcgtat ttccacggga aaattgaacc gatggcttga aacgatcgtt gcgcatcatc ccccgcctgc ggttttgggg cgtcgactta aggtcaaata tgtcacacag gttaagacac gtcctcctgg atttgtgttt tcttgctcgc gaccaaagct catgcctcaa tcgtatttac gttatctttc taatgaattg cgtaatacgt ttgatatgcc tgggatacca attcgcatat cattacgggc atctgataat ccctttgcaa cgcgttccaa aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLTIAIVG RPNVGKSTLF NRLVGQKLAL VDDKPGVTRD RRVHAATLQD LRFDVIDTAG LEETGDHTLE GRMRAHTKAA IDEADLILFV FDAKSGITPS DLNFASLVRK SGKPIVLVAN KSESKAAIGG EYEAWSLGLG EPCSISAEHG LGLSDLRDAI MKASGGERIF ASKNQEEHIA LQSASLDDYI DNLEEKGDVY DKSKPLRIAV AGRPNTGKST LINRMLGQDR LLTGPEAGLT RDSISVDWEW RGRHIKLFDT AGLRRKSKIQ EKLEKLSVAD TLRAIRFAEV VVIVFDATTP FEKQDLQIAD LVIREGRVPL IAFNKWDLIE NSQATLVDLH EKCIRLLPQV RGLRAVPLSG QYGQGIDKLM ENVMMMHRVW NRRISTGKLN RWLETIVAHH PPPAVLGRRL KVKYVTQVKT RPPGFVFSCS RPKLMPQSYL RYLSNELRNT FDMPGIPIRI SLRASDNPFA TRSKKK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCTTACC ATTGCTATAG TTGGTCGTCC TAATGTTGGA AAGTCAACGC TTTTTAATCG TTTGGTTGGG CAAAAATTGG CTTTGGTTGA TGATAAGCCT GGTGTGACAC GCGATCGGCG TGTTCATGCT GCAACACTTC AAGATCTGCG TTTTGATGTG ATTGATACGG CTGGGTTGGA AGAAACCGGT GATCATACAC TCGAAGGTCG TATGCGTGCT CATACAAAAG CTGCTATCGA TGAAGCGGAT CTTATTTTAT TTGTATTTGA TGCAAAGAGT GGAATAACGC CGAGTGATTT AAATTTTGCG TCACTGGTTC GCAAATCAGG CAAGCCGATT GTGCTTGTTG CCAATAAATC TGAGTCAAAA GCGGCAATTG GGGGAGAATA TGAGGCATGG TCGTTAGGGT TGGGGGAGCC TTGTTCAATA TCTGCTGAAC ATGGTTTAGG TCTTTCGGAT CTTCGTGATG CAATTATGAA GGCTAGTGGG GGAGAGAGAA TTTTTGCAAG CAAAAACCAA GAAGAACATA TTGCTTTACA GTCAGCATCT CTTGATGATT ATATTGATAA TTTAGAAGAA AAGGGGGATG TTTACGATAA AAGTAAACCC CTTCGGATTG CAGTTGCTGG TCGCCCAAAC ACCGGAAAAT CAACTCTCAT CAATAGGATG TTAGGGCAAG ATCGTTTGTT GACAGGACCT GAAGCAGGAT TAACTCGTGA TTCCATTTCT GTGGATTGGG AATGGCGCGG TCGTCATATT AAGCTTTTTG ATACTGCAGG TTTACGTCGA AAGTCGAAAA TTCAAGAAAA ATTAGAAAAA CTTTCTGTTG CAGATACTTT GCGTGCAATT CGTTTTGCGG AAGTGGTAGT AATTGTTTTT GATGCAACTA CACCTTTTGA AAAGCAGGAT TTACAAATTG CTGATCTTGT GATTCGTGAA GGGCGTGTTC CACTTATTGC TTTCAATAAG TGGGATCTTA TTGAAAATAG TCAGGCAACA TTGGTTGATT TGCATGAAAA ATGCATCCGC CTTCTTCCTC AAGTTCGTGG TTTGAGAGCT GTTCCTTTAT CGGGTCAATA TGGTCAAGGG ATTGATAAAT TGATGGAAAA TGTCATGATG ATGCACCGTG TGTGGAATCG TCGTATTTCC ACGGGAAAAT TGAACCGATG GCTTGAAACG ATCGTTGCGC ATCATCCCCC GCCTGCGGTT TTGGGGCGTC GACTTAAGGT CAAATATGTC ACACAGGTTA AGACACGTCC TCCTGGATTT GTGTTTTCTT GCTCGCGACC AAAGCTCATG CCTCAATCGT ATTTACGTTA TCTTTCTAAT GAATTGCGTA ATACGTTTGA TATGCCTGGG ATACCAATTC GCATATCATT ACGGGCATCT GATAATCCCT TTGCAACGCG TTCCAAAAAG AAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA