BaheA.00784.a

Thioredoxin reductase

CENTER ID: BaheA.00784.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00784.a.A1.GE25885 Full length( BaheA.00784.a ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00784.a.A1.PW25944 Full length( BaheA.00784.a ) 1 317

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1219
RefSeq: YP_033931.1
UniProt: A0A0H3LXW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVQSHVRLLI IGSGPAGYTA AIYAARGMLK PVLITGLQQG GQLMITTDVE NYPGFADPIQ GPWLMEQMAK QAENMGTKIV YDNITKADLS KRPFILYGDS GAQYCCDALI IATGAQARWL GLESEQVFMG GGVSACATCD GFFYRDKDVI VVGGGNTAVE EALYLSHLAK RVSVVHRRDY FRAEKILQDR LFARDNVCVL WDHVVEEIIG LPARASIGAV VTGVRLKNVK TGQKVHVDTD GIFIAIGHDP AVSLFEGQLK QKSGGYLWTA PDSTATSIAG VFAAGDVVDD VFRQAITAAG RGCMAALEAE RFLELQI
NT Sequence
ATGGTGCAGT CGCATGTTCG TCTGCTTATT ATTGGTTCTG GTCCAGCTGG TTATACAGCG GCGATCTATG CAGCGAGAGG AATGCTCAAA CCGGTTTTGA TTACTGGATT ACAGCAAGGC GGGCAGTTGA TGATTACAAC TGATGTCGAG AATTATCCAG GTTTTGCTGA TCCAATTCAG GGACCATGGT TGATGGAACA AATGGCAAAA CAAGCCGAAA ATATGGGCAC GAAAATTGTC TATGATAATA TTACAAAGGC TGATTTATCG AAGCGCCCTT TTATCTTATA CGGAGATTCA GGAGCGCAAT ATTGTTGTGA TGCGCTCATT ATTGCAACCG GAGCGCAGGC GCGCTGGCTT GGTTTGGAGA GTGAACAGGT ATTTATGGGT GGGGGTGTTT CTGCTTGCGC TACATGTGAT GGTTTCTTTT ATCGTGATAA GGACGTTATT GTTGTGGGAG GGGGAAATAC AGCCGTTGAA GAAGCCCTTT ATTTATCGCA TTTGGCGAAG AGGGTAAGCG TAGTTCATCG GCGGGATTAT TTTCGGGCAG AAAAAATTTT GCAAGATAGG TTATTTGCCC GTGATAATGT GTGTGTTCTT TGGGATCATG TGGTAGAAGA AATTATTGGT TTGCCAGCTC GTGCTTCAAT AGGAGCTGTT GTGACAGGTG TACGTCTTAA AAATGTTAAA ACCGGTCAAA AAGTTCATGT GGATACGGAT GGAATATTTA TTGCTATCGG ACATGACCCT GCTGTCTCTT TGTTTGAAGG TCAACTAAAA CAAAAATCAG GTGGCTACTT ATGGACAGCG CCGGATTCAA CAGCAACAAG CATTGCTGGT GTTTTTGCTG CGGGAGATGT CGTAGACGAC GTATTTCGTC AGGCAATAAC AGCAGCGGGG AGGGGATGTA TGGCAGCATT GGAGGCAGAA CGATTTTTGG AGTTGCAGAT A
Details for BaheA.00784.a.A1.GE25885
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSHVRLL IIGSGPAGYT AAIYAARGML KPVLITGLQQ GGQLMITTDV ENYPGFADPI QGPWLMEQMA KQAENMGTKI VYDNITKADL SKRPFILYGD SGAQYCCDAL IIATGAQARW LGLESEQVFM GGGVSACATC DGFFYRDKDV IVVGGGNTAV EEALYLSHLA KRVSVVHRRD YFRAEKILQD RLFARDNVCV LWDHVVEEII GLPARASIGA VVTGVRLKNV KTGQKVHVDT DGIFIAIGHD PAVSLFEGQL KQKSGGYLWT APDSTATSIA GVFAAGDVVD DVFRQAITAA GRGCMAALEA ERFLE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc agtcgcatgt tcgtctgctt attattggtt ctggtccagc tggttataca gcggcgatct atgcagcgag aggaatgctc aaaccggttt tgattactgg attacagcaa ggcgggcagt tgatgattac aactgatgtc gagaattatc caggttttgc tgatccaatt cagggaccat ggttgatgga acaaatggca aaacaagccg aaaatatggg cacgaaaatt gtctatgata atattacaaa ggctgattta tcgaagcgcc cttttatctt atacggagat tcaggagcgc aatattgttg tgatgcgctc attattgcaa ccggagcgca ggcgcgctgg cttggtttgg agagtgaaca ggtatttatg ggtgggggtg tttctgcttg cgctacatgt gatggtttct tttatcgtga taaggacgtt attgttgtgg gagggggaaa tacagccgtt gaagaagccc tttatttatc gcatttggcg aagagggtaa gcgtagttca tcggcgggat tattttcggg cagaaaaaat tttgcaagat aggttatttg cccgtgataa tgtgtgtgtt ctttgggatc atgtggtaga agaaattatt ggtttgccag ctcgtgcttc aataggagct gttgtgacag gtgtacgtct taaaaatgtt aaaaccggtc aaaaagttca tgtggatacg gatggaatat ttattgctat cggacatgac cctgctgtct ctttgtttga aggtcaacta aaacaaaaat caggtggcta cttatggaca gcgccggatt caacagcaac aagcattgct ggtgtttttg ctgcgggaga tgtcgtagac gacgtatttc gtcaggcaat aacagcagcg gggaggggat gtatggcagc attggaggca gaacgatttt tggagttg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSHVRLL IIGSGPAGYT AAIYAARGML KPVLITGLQQ GGQLMITTDV ENYPGFADPI QGPWLMEQMA KQAENMGTKI VYDNITKADL SKRPFILYGD SGAQYCCDAL IIATGAQARW LGLESEQVFM GGGVSACATC DGFFYRDKDV IVVGGGNTAV EEALYLSHLA KRVSVVHRRD YFRAEKILQD RLFARDNVCV LWDHVVEEII GLPARASIGA VVTGVRLKNV KTGQKVHVDT DGIFIAIGHD PAVSLFEGQL KQKSGGYLWT APDSTATSIA GVFAAGDVVD DVFRQAITAA GRGCMAALEA ERFLELQI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcagtcg catgttcgtc tgcttattat tggttctggt ccagctggtt atacagcggc gatctatgca gcgagaggaa tgctcaaacc ggttttgatt actggattac agcaaggcgg gcagttgatg attacaactg atgtcgagaa ttatccaggt tttgctgatc caattcaggg accatggttg atggaacaaa tggcaaaaca agccgaaaat atgggcacga aaattgtcta tgataatatt acaaaggctg atttatcgaa gcgccctttt atcttatacg gagattcagg agcgcaatat tgttgtgatg cgctcattat tgcaaccgga gcgcaggcgc gctggcttgg tttggagagt gaacaggtat ttatgggtgg gggtgtttct gcttgcgcta catgtgatgg tttcttttat cgtgataagg acgttattgt tgtgggaggg ggaaatacag ccgttgaaga agccctttat ttatcgcatt tggcgaagag ggtaagcgta gttcatcggc gggattattt tcgggcagaa aaaattttgc aagataggtt atttgcccgt gataatgtgt gtgttctttg ggatcatgtg gtagaagaaa ttattggttt gccagctcgt gcttcaatag gagctgttgt gacaggtgta cgtcttaaaa atgttaaaac cggtcaaaaa gttcatgtgg atacggatgg aatatttatt gctatcggac atgaccctgc tgtctctttg tttgaaggtc aactaaaaca aaaatcaggt ggctacttat ggacagcgcc ggattcaaca gcaacaagca ttgctggtgt ttttgctgcg ggagatgtcg tagacgacgt atttcgtcag gcaataacag cagcggggag gggatgtatg gcagcattgg aggcagaacg atttttggag ttgcagataa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BaheA.00784.a.A1.PW25944
PURIFICATION DATe: 2/10/2009
CONCENTRATION: 29.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 23
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSHVRLL IIGSGPAGYT AAIYAARGML KPVLITGLQQ GGQLMITTDV ENYPGFADPI QGPWLMEQMA KQAENMGTKI VYDNITKADL SKRPFILYGD SGAQYCCDAL IIATGAQARW LGLESEQVFM GGGVSACATC DGFFYRDKDV IVVGGGNTAV EEALYLSHLA KRVSVVHRRD YFRAEKILQD RLFARDNVCV LWDHVVEEII GLPARASIGA VVTGVRLKNV KTGQKVHVDT DGIFIAIGHD PAVSLFEGQL KQKSGGYLWT APDSTATSIA GVFAAGDVVD DVFRQAITAA GRGCMAALEA ERFLE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc agtcgcatgt tcgtctgctt attattggtt ctggtccagc tggttataca gcggcgatct atgcagcgag aggaatgctc aaaccggttt tgattactgg attacagcaa ggcgggcagt tgatgattac aactgatgtc gagaattatc caggttttgc tgatccaatt cagggaccat ggttgatgga acaaatggca aaacaagccg aaaatatggg cacgaaaatt gtctatgata atattacaaa ggctgattta tcgaagcgcc cttttatctt atacggagat tcaggagcgc aatattgttg tgatgcgctc attattgcaa ccggagcgca ggcgcgctgg cttggtttgg agagtgaaca ggtatttatg ggtgggggtg tttctgcttg cgctacatgt gatggtttct tttatcgtga taaggacgtt attgttgtgg gagggggaaa tacagccgtt gaagaagccc tttatttatc gcatttggcg aagagggtaa gcgtagttca tcggcgggat tattttcggg cagaaaaaat tttgcaagat aggttatttg cccgtgataa tgtgtgtgtt ctttgggatc atgtggtaga agaaattatt ggtttgccag ctcgtgcttc aataggagct gttgtgacag gtgtacgtct taaaaatgtt aaaaccggtc aaaaagttca tgtggatacg gatggaatat ttattgctat cggacatgac cctgctgtct ctttgtttga aggtcaacta aaacaaaaat caggtggcta cttatggaca gcgccggatt caacagcaac aagcattgct ggtgtttttg ctgcgggaga tgtcgtagac gacgtatttc gtcaggcaat aacagcagcg gggaggggat gtatggcagc attggaggca gaacgatttt tggagttg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSHVRLL IIGSGPAGYT AAIYAARGML KPVLITGLQQ GGQLMITTDV ENYPGFADPI QGPWLMEQMA KQAENMGTKI VYDNITKADL SKRPFILYGD SGAQYCCDAL IIATGAQARW LGLESEQVFM GGGVSACATC DGFFYRDKDV IVVGGGNTAV EEALYLSHLA KRVSVVHRRD YFRAEKILQD RLFARDNVCV LWDHVVEEII GLPARASIGA VVTGVRLKNV KTGQKVHVDT DGIFIAIGHD PAVSLFEGQL KQKSGGYLWT APDSTATSIA GVFAAGDVVD DVFRQAITAA GRGCMAALEA ERFLELQI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCAGTCG CATGTTCGTC TGCTTATTAT TGGTTCTGGT CCAGCTGGTT ATACAGCGGC GATCTATGCA GCGAGAGGAA TGCTCAAACC GGTTTTGATT ACTGGATTAC AGCAAGGCGG GCAGTTGATG ATTACAACTG ATGTCGAGAA TTATCCAGGT TTTGCTGATC CAATTCAGGG ACCATGGTTG ATGGAACAAA TGGCAAAACA AGCCGAAAAT ATGGGCACGA AAATTGTCTA TGATAATATT ACAAAGGCTG ATTTATCGAA GCGCCCTTTT ATCTTATACG GAGATTCAGG AGCGCAATAT TGTTGTGATG CGCTCATTAT TGCAACCGGA GCGCAGGCGC GCTGGCTTGG TTTGGAGAGT GAACAGGTAT TTATGGGTGG GGGTGTTTCT GCTTGCGCTA CATGTGATGG TTTCTTTTAT CGTGATAAGG ACGTTATTGT TGTGGGAGGG GGAAATACAG CCGTTGAAGA AGCCCTTTAT TTATCGCATT TGGCGAAGAG GGTAAGCGTA GTTCATCGGC GGGATTATTT TCGGGCAGAA AAAATTTTGC AAGATAGGTT ATTTGCCCGT GATAATGTGT GTGTTCTTTG GGATCATGTG GTAGAAGAAA TTATTGGTTT GCCAGCTCGT GCTTCAATAG GAGCTGTTGT GACAGGTGTA CGTCTTAAAA ATGTTAAAAC CGGTCAAAAA GTTCATGTGG ATACGGATGG AATATTTATT GCTATCGGAC ATGACCCTGC TGTCTCTTTG TTTGAAGGTC AACTAAAACA AAAATCAGGT GGCTACTTAT GGACAGCGCC GGATTCAACA GCAACAAGCA TTGCTGGTGT TTTTGCTGCG GGAGATGTCG TAGACGACGT ATTTCGTCAG GCAATAACAG CAGCGGGGAG GGGATGTATG GCAGCATTGG AGGCAGAACG ATTTTTGGAG TTGCAGATAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A