SSGCID: Target Page

BaheA.00771.a

Diaminopimelate decarboxylase

CENTER ID:	BaheA.00771.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00771.a.B1.GE27110	Full length( BaheA.00771.a )		1	421

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1879
RefSeq:	YP_034310.1
UniProt:	A0A0H3M000

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFFSYNQGV LHAEGLSLSA LAQDVGTPFY CYSASAVVTN FKDYQNAFLD MPSLIAYAVK ANSNQAVLRL LAANGAGADV VSEGELRRAL AAGIPAHRIV YSGVGKTVKE IDFALAHDIY CFNVESEPEL EQLSVCAVAL SKTARVSLRI NPDVDARTHR KITTGKSENK FGIPLSLAWD VYKKAAQLPG IQVCGVDMHI GSQICDLKPF EDAFLIVADF VRQLRSHGYA LTHIDIGGGL GIDYGNEHHS VPSLSDYAAL VRKHIAPLGI HIILEPGRSI VGDAGVLVTS VLYLKRGNGR NFVIVDAAMN DLMRPTLYDA WQNVLLVEQA SKDALLMKAD IVGAVCETGD YLALDRRVPT LATGDLLAIM EAGAYGAVMA STYNSRLLVP EVLVQDTRYA VIRPRLDYAQ LIGCDYVPDW I

NT Sequence

ATGCGTTTTT TTTCTTATAA TCAGGGTGTC CTTCACGCTG AAGGTCTTTC TCTTTCTGCA TTGGCGCAGG ATGTGGGAAC ACCTTTTTAT TGTTATTCAG CCAGTGCAGT GGTGACAAAT TTTAAAGACT ATCAAAACGC CTTTCTGGAT ATGCCTAGCT TGATTGCTTA TGCAGTTAAG GCTAATTCTA ATCAAGCAGT GTTACGGCTT TTAGCGGCAA ACGGAGCGGG AGCTGATGTG GTTTCAGAAG GTGAATTGCG ACGCGCACTT GCTGCTGGTA TTCCTGCTCA TCGTATTGTT TATTCTGGTG TTGGCAAAAC AGTAAAGGAG ATAGATTTTG CTCTTGCACA CGATATTTAC TGTTTTAACG TTGAATCAGA GCCTGAACTT GAACAACTTT CGGTATGTGC TGTTGCGCTT TCAAAAACAG CACGCGTTTC ATTGCGCATT AATCCAGATG TAGATGCAAG GACTCATAGA AAAATTACAA CGGGGAAATC TGAAAATAAA TTTGGTATTC CACTGTCTTT GGCGTGGGAT GTATATAAAA AAGCAGCTCA ATTACCAGGC ATTCAGGTGT GTGGGGTAGA TATGCATATT GGCAGTCAGA TCTGTGATCT GAAACCGTTT GAAGATGCAT TTCTTATTGT TGCAGATTTT GTGCGTCAGT TAAGGAGTCA TGGCTATGCA CTTACCCATA TCGATATTGG GGGCGGTCTT GGTATTGACT ATGGGAATGA ACATCATTCA GTACCATCTC TTTCTGATTA TGCAGCACTT GTAAGGAAGC ATATTGCGCC TTTAGGGATT CATATTATCC TGGAGCCTGG GCGCAGTATT GTCGGCGATG CTGGCGTGTT GGTGACCTCT GTTCTCTACT TAAAAAGAGG GAATGGACGC AATTTTGTTA TTGTGGATGC GGCAATGAAT GATCTTATGC GTCCAACACT CTATGATGCT TGGCAAAATG TACTACTTGT GGAACAAGCA TCAAAAGATG CATTACTTAT GAAAGCAGAT ATTGTTGGTG CAGTTTGTGA AACCGGTGAT TATCTAGCAT TAGATCGTCG TGTACCGACC TTGGCGACAG GTGATCTTTT AGCCATTATG GAGGCTGGTG CTTACGGTGC TGTGATGGCA AGCACTTATA ATAGCCGACT TTTGGTGCCA GAAGTTCTCG TTCAGGATAC GCGTTATGCG GTTATTCGTC CACGTCTTGA TTATGCACAA TTGATAGGCT GTGATTATGT TCCAGATTGG ATT

Details for BaheA.00771.a.B1.GE27110

HARVESTED ON:	8/17/2009
SEQUENCED ON:	10/20/2009
EXPECTED MW:	46kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	13

Validated AA Sequence

MPSLIAYAVK ANSNQAVLRL LXXNGAGADV VSEGELXXAL AAGIPAHRIV YSGVGXTXK

Validated NT Sequence

tangcgtttt ttttcttata atcngggtgt ccttcacgct gaaggtcttt ctctttctgc attggcgcag gatgtgggaa caccttttta ttgttattca gccagtgcag tggtgacaaa ttttaaagac tatcaaaacg cctttctgga tatgcctagc ttgattgctt atgcagttaa ggctaattct aatcaagcag tgttacggct tttancgnnn aacggagcgg gagctgatgt ggtttcagaa ggtgaattgn nacncgcact tgctgctggt attcctgctc atcgtattgt ttattctggt gttggnnaaa cantaaagga

Expected Protein Sequence

MAHHHHHHMR FFSYNQGVLH AEGLSLSALA QDVGTPFYCY SASAVVTNFK DYQNAFLDMP SLIAYAVKAN SNQAVLRLLA ANGAGADVVS EGELRRALAA GIPAHRIVYS GVGKTVKEID FALAHDIYCF NVESEPELEQ LSVCAVALSK TARVSLRINP DVDARTHRKI TTGKSENKFG IPLSLAWDVY KKAAQLPGIQ VCGVDMHIGS QICDLKPFED AFLIVADFVR QLRSHGYALT HIDIGGGLGI DYGNEHHSVP SLSDYAALVR KHIAPLGIHI ILEPGRSIVG DAGVLVTSVL YLKRGNGRNF VIVDAAMNDL MRPTLYDAWQ NVLLVEQASK DALLMKADIV GAVCETGDYL ALDRRVPTLA TGDLLAIMEA GAYGAVMAST YNSRLLVPEV LVQDTRYAVI RPRLDYAQLI GCDYVPDWI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt ttttttctta taatcagggt gtccttcacg ctgaaggtct ttctctttct gcattggcgc aggatgtggg aacacctttt tattgttatt cagccagtgc agtggtgaca aattttaaag actatcaaaa cgcctttctg gatatgccta gcttgattgc ttatgcagtt aaggctaatt ctaatcaagc agtgttacgg cttttagcgg caaacggagc gggagctgat gtggtttcag aaggtgaatt gcgacgcgca cttgctgctg gtattcctgc tcatcgtatt gtttattctg gtgttggcaa aacagtaaag gagatagatt ttgctcttgc acacgatatt tactgtttta acgttgaatc agagcctgaa cttgaacaac tttcggtatg tgctgttgcg ctttcaaaaa cagcacgcgt ttcattgcgc attaatccag atgtagatgc aaggactcat agaaaaatta caacggggaa atctgaaaat aaatttggta ttccactgtc tttggcgtgg gatgtatata aaaaagcagc tcaattacca ggcattcagg tgtgtggggt agatatgcat attggcagtc agatctgtga tctgaaaccg tttgaagatg catttcttat tgttgcagat tttgtgcgtc agttaaggag tcatggctat gcacttaccc atatcgatat tgggggcggt cttggtattg actatgggaa tgaacatcat tcagtaccat ctctttctga ttatgcagca cttgtaagga agcatattgc gcctttaggg attcatatta tcctggagcc tgggcgcagt attgtcggcg atgctggcgt gttggtgacc tctgttctct acttaaaaag agggaatgga cgcaattttg ttattgtgga tgcggcaatg aatgatctta tgcgtccaac actctatgat gcttggcaaa atgtactact tgtggaacaa gcatcaaaag atgcattact tatgaaagca gatattgttg gtgcagtttg tgaaaccggt gattatctag cattagatcg tcgtgtaccg accttggcga caggtgatct tttagccatt atggaggctg gtgcttacgg tgctgtgatg gcaagcactt ataatagccg acttttggtg ccagaagttc tcgttcagga tacgcgttat gcggttattc gtccacgtct tgattatgca caattgatag gctgtgatta tgttccagat tggatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t