BaheA.00763.a

riboflavin synthase subunit alpha

CENTER ID: BaheA.00763.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00763.a.A1.GE25809 Full length( BaheA.00763.a ) 1 206
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00763.a.A1.PS00159 Full length( BaheA.00763.a ) 1 206

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.810
RefSeq: YP_033569.1
UniProt: Q9REF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTGIVTDIG CVEDIQSLKQ GMRFNISTRY SIENLEIGAS IACSGVCLTI VERGSKQQAK INWFAVEAWE EALRLTNLTQ WTKGTFINLE RSLRLGDEMG GHLVSGHIDG LAEIIDQKSE GDAVRFFLQV PKRFIPFIVN KGSIALNGTS LTVNCVEEHI FDVLIIRHTL EMTTWRQAKI GDLVNLEIDQ LARYVAKLSG FRIKDE
NT Sequence
ATGTTCACAG GAATTGTAAC GGATATCGGT TGTGTTGAAG ATATTCAATC CTTAAAGCAG GGGATGCGAT TTAATATTTC TACACGTTAT AGTATCGAAA ACTTAGAAAT TGGTGCATCA ATTGCGTGTT CAGGAGTTTG TCTAACAATT GTTGAGCGAG GATCAAAACA ACAAGCGAAG ATCAATTGGT TTGCTGTAGA AGCGTGGGAA GAAGCGTTGC GTTTGACTAA TCTTACACAA TGGACGAAAG GAACATTTAT TAATTTAGAA CGTTCGCTAC GATTGGGTGA TGAAATGGGT GGGCATTTGG TTTCTGGTCA TATTGATGGT TTGGCTGAGA TTATTGATCA GAAAAGCGAA GGTGATGCAG TTCGTTTTTT CCTACAAGTA CCAAAGCGTT TTATACCTTT TATTGTAAAT AAAGGTTCTA TCGCACTTAA TGGAACATCT TTGACCGTTA ATTGTGTTGA AGAGCATATT TTCGATGTTC TTATTATTCG CCATACACTT GAAATGACAA CCTGGAGACA AGCTAAAATT GGAGATCTGG TCAATTTAGA AATTGATCAA CTTGCCCGTT ATGTTGCAAA ACTTTCTGGC TTTAGAATAA AAGATGAA
Details for BaheA.00763.a.A1.GE25809
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFTGIVTDI GCVEDIQSLK QGMRFNISTR YSIENLEIGA SIACSGVCLT IVERGSKQQA KINWFAVEAW EEALRLTNLT QWTKGTFINL ERSLRLGDEM GGHLVSGHID GLAEIIDQKS EGDAVRFFLQ VPKRFIPFIV NKGSIALNGT SLTVNCVEEH IFDVLIIRHT LEMTTWRQAK IGDLVNLEID QLARYVAKLS GFRIK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca caggaattgt aacggatatc ggttgtgttg aagatattca atccttaaag caggggatgc gatttaatat ttctacacgt tatagtatcg aaaacttaga aattggtgca tcaattgcgt gttcaggagt ttgtctaaca attgttgagc gaggatcaaa acaacaagcg aagatcaatt ggtttgctgt agaagcgtgg gaagaagcgt tgcgtttgac taatcttaca caatggacga aaggaacatt tattaattta gaacgttcgc tacgattggg tgatgaaatg ggtgggcatt tggtttctgg tcatattgat ggtttggctg agattattga tcagaaaagc gaaggtgatg cagttcgttt tttcctacaa gtaccaaagc gttttatacc ttttattgta aataaaggtt ctatcgcact taatggaaca tctttgaccg ttaattgtgt tgaagagcat attttcgatg ttcttattat tcgccataca cttgaaatga caacctggag acaagctaaa attggagatc tggtcaattt agaaattgat caacttgccc gttatgttgc aaaactttct ggctttagaa taaaag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFTGIVTDI GCVEDIQSLK QGMRFNISTR YSIENLEIGA SIACSGVCLT IVERGSKQQA KINWFAVEAW EEALRLTNLT QWTKGTFINL ERSLRLGDEM GGHLVSGHID GLAEIIDQKS EGDAVRFFLQ VPKRFIPFIV NKGSIALNGT SLTVNCVEEH IFDVLIIRHT LEMTTWRQAK IGDLVNLEID QLARYVAKLS GFRIKDE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcacagga attgtaacgg atatcggttg tgttgaagat attcaatcct taaagcaggg gatgcgattt aatatttcta cacgttatag tatcgaaaac ttagaaattg gtgcatcaat tgcgtgttca ggagtttgtc taacaattgt tgagcgagga tcaaaacaac aagcgaagat caattggttt gctgtagaag cgtgggaaga agcgttgcgt ttgactaatc ttacacaatg gacgaaagga acatttatta atttagaacg ttcgctacga ttgggtgatg aaatgggtgg gcatttggtt tctggtcata ttgatggttt ggctgagatt attgatcaga aaagcgaagg tgatgcagtt cgttttttcc tacaagtacc aaagcgtttt atacctttta ttgtaaataa aggttctatc gcacttaatg gaacatcttt gaccgttaat tgtgttgaag agcatatttt cgatgttctt attattcgcc atacacttga aatgacaacc tggagacaag ctaaaattgg agatctggtc aatttagaaa ttgatcaact tgcccgttat gttgcaaaac tttctggctt tagaataaaa gatgaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BaheA.00763.a.A1.PS00159
PURIFICATION DATe: 2/6/2009
CONCENTRATION: 29.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFTGIVTDI GCVEDIQSLK QGMRFNISTR YSIENLEIGA SIACSGVCLT IVERGSKQQA KINWFAVEAW EEALRLTNLT QWTKGTFINL ERSLRLGDEM GGHLVSGHID GLAEIIDQKS EGDAVRFFLQ VPKRFIPFIV NKGSIALNGT SLTVNCVEEH IFDVLIIRHT LEMTTWRQAK IGDLVNLEID QLARYVAKLS GFRIK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca caggaattgt aacggatatc ggttgtgttg aagatattca atccttaaag caggggatgc gatttaatat ttctacacgt tatagtatcg aaaacttaga aattggtgca tcaattgcgt gttcaggagt ttgtctaaca attgttgagc gaggatcaaa acaacaagcg aagatcaatt ggtttgctgt agaagcgtgg gaagaagcgt tgcgtttgac taatcttaca caatggacga aaggaacatt tattaattta gaacgttcgc tacgattggg tgatgaaatg ggtgggcatt tggtttctgg tcatattgat ggtttggctg agattattga tcagaaaagc gaaggtgatg cagttcgttt tttcctacaa gtaccaaagc gttttatacc ttttattgta aataaaggtt ctatcgcact taatggaaca tctttgaccg ttaattgtgt tgaagagcat attttcgatg ttcttattat tcgccataca cttgaaatga caacctggag acaagctaaa attggagatc tggtcaattt agaaattgat caacttgccc gttatgttgc aaaactttct ggctttagaa taaaag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFTGIVTDI GCVEDIQSLK QGMRFNISTR YSIENLEIGA SIACSGVCLT IVERGSKQQA KINWFAVEAW EEALRLTNLT QWTKGTFINL ERSLRLGDEM GGHLVSGHID GLAEIIDQKS EGDAVRFFLQ VPKRFIPFIV NKGSIALNGT SLTVNCVEEH IFDVLIIRHT LEMTTWRQAK IGDLVNLEID QLARYVAKLS GFRIKDE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCACAGGA ATTGTAACGG ATATCGGTTG TGTTGAAGAT ATTCAATCCT TAAAGCAGGG GATGCGATTT AATATTTCTA CACGTTATAG TATCGAAAAC TTAGAAATTG GTGCATCAAT TGCGTGTTCA GGAGTTTGTC TAACAATTGT TGAGCGAGGA TCAAAACAAC AAGCGAAGAT CAATTGGTTT GCTGTAGAAG CGTGGGAAGA AGCGTTGCGT TTGACTAATC TTACACAATG GACGAAAGGA ACATTTATTA ATTTAGAACG TTCGCTACGA TTGGGTGATG AAATGGGTGG GCATTTGGTT TCTGGTCATA TTGATGGTTT GGCTGAGATT ATTGATCAGA AAAGCGAAGG TGATGCAGTT CGTTTTTTCC TACAAGTACC AAAGCGTTTT ATACCTTTTA TTGTAAATAA AGGTTCTATC GCACTTAATG GAACATCTTT GACCGTTAAT TGTGTTGAAG AGCATATTTT CGATGTTCTT ATTATTCGCC ATACACTTGA AATGACAACC TGGAGACAAG CTAAAATTGG AGATCTGGTC AATTTAGAAA TTGATCAACT TGCCCGTTAT GTTGCAAAAC TTTCTGGCTT TAGAATAAAA GATGAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA