BaheA.00757.a

Oxidoreductase

CENTER ID:	BaheA.00757.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00757.a.A1.GE25807	Full length( BaheA.00757.a )		1	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_144629
PATRIC ID:	fig\|283166.5.peg.569
RefSeq:	YP_033359.1
UniProt:	A0A0H3M2Y6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKLDFSLFG RVALVTGASR GIGYHLALEL AERGAHIIAL ARTMSGLTEL DNQIQKKGAR ATLIPIDLHH MENIDALATS ITQDWKKLDI MIANAGILGT LSPIAHIENK VFEDIFQINL ISQWRLMKAV EPLLRKSDAG RVILLSSSVA HVARAFWGPY AASKAALEII SRCWAEELKQ TPIKINCVDP GATRTAMRAQ AMPGEDPQTL PPPQEVAEKI AHLLSPDLKE TGKLFNVRKN RFMNYHAPN

NT Sequence

ATGAAAAAAC TTGATTTTAG TCTCTTTGGT CGTGTTGCAC TCGTTACCGG TGCTTCAAGA GGAATTGGAT ATCATTTAGC ATTAGAGCTT GCAGAACGTG GCGCTCACAT TATTGCTCTT GCACGAACAA TGAGTGGGCT CACTGAACTT GACAATCAAA TCCAAAAAAA AGGTGCTCGC GCAACACTCA TTCCAATCGA CTTACATCAT ATGGAAAACA TTGATGCTCT TGCTACTTCA ATTACTCAGG ATTGGAAAAA ACTTGATATT ATGATCGCAA ATGCAGGAAT CCTTGGAACT CTTTCACCAA TAGCTCACAT AGAAAATAAG GTATTTGAAG ATATTTTTCA AATAAATCTT ATCAGCCAAT GGCGCTTAAT GAAAGCTGTT GAACCCTTAT TGCGTAAATC TGATGCTGGA CGAGTAATTC TTTTATCTTC AAGCGTTGCT CATGTTGCCC GCGCTTTCTG GGGACCCTAT GCAGCCTCTA AAGCGGCTCT AGAAATTATT TCCCGTTGTT GGGCAGAAGA ATTAAAACAA ACCCCAATCA AGATTAATTG TGTTGATCCC GGTGCAACAC GCACTGCCAT GCGCGCCCAA GCTATGCCTG GGGAAGATCC TCAAACTCTT CCTCCACCAC AAGAAGTTGC AGAAAAGATA GCACATTTAC TCTCACCTGA TTTAAAGGAA ACCGGCAAAC TCTTTAATGT GCGTAAAAAT CGATTCATGA ATTATCACGC ACCTAAT

Details for BaheA.00757.a.A1.GE25807

HARVESTED ON:	10/31/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMKKLDFS LFGRVALVTG ASRGIGYHLA LELAERGAHI IALARTMSGL TELDNQIQKK GARATLIPID LHHMENIDAL ATSITQDWKK LDIMIANAGI LGTLSPIAHI ENKVFEDIFQ INLISQWRLM KAVEPLLRKS DAGRVILLSS SVAHVARAFW GPYAASKAAL EIISRCWAEE LKQTPIKINC VDPGATRTAM RAQAMPGEDP QTLPPPQEVA EKIAHLLSPD LKETGKLFNV RKNRFMNYHA PN

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaaaaa acttgatttt agtctctttg gtcgtgttgc actcgttacc ggtgcttcaa gaggaattgg atatcattta gcattagagc ttgcagaacg tggcgctcac attattgctc ttgcacgaac aatgagtggg ctcactgaac ttgacaatca aatccaaaaa aaaggtgctc gcgcaacact cattccaatc gacttacatc atatggaaaa cattgatgct cttgctactt caattactca ggattggaaa aaacttgata ttatgatcgc aaatgcagga atccttggaa ctctttcacc aatagctcac atagaaaata aggtatttga agatattttt caaataaatc ttatcagcca atggcgctta atgaaagctg ttgaaccctt attgcgtaaa tctgatgctg gacgagtaat tcttttatct tcaagcgttg ctcatgttgc ccgcgctttc tggggaccct atgcagcctc taaagcggct ctagaaatta tttcccgttg ttgggcagaa gaattaaaac aaaccccaat caagattaat tgtgttgatc ccggtgcaac acgcactgcc atgcgcgccc aagctatgcc tggggaagat cctcaaactc ttcctccacc acaagaagtt gcagaaaaga tagcacattt actctcacct gatttaaagg aaaccggcaa actctttaat gtgcgtaaaa atcgattcat gaattatcac gcacctaatt aataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKKLDFSLF GRVALVTGAS RGIGYHLALE LAERGAHIIA LARTMSGLTE LDNQIQKKGA RATLIPIDLH HMENIDALAT SITQDWKKLD IMIANAGILG TLSPIAHIEN KVFEDIFQIN LISQWRLMKA VEPLLRKSDA GRVILLSSSV AHVARAFWGP YAASKAALEI ISRCWAEELK QTPIKINCVD PGATRTAMRA QAMPGEDPQT LPPPQEVAEK IAHLLSPDLK ETGKLFNVRK NRFMNYHAPN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaaaactt gattttagtc tctttggtcg tgttgcactc gttaccggtg cttcaagagg aattggatat catttagcat tagagcttgc agaacgtggc gctcacatta ttgctcttgc acgaacaatg agtgggctca ctgaacttga caatcaaatc caaaaaaaag gtgctcgcgc aacactcatt ccaatcgact tacatcatat ggaaaacatt gatgctcttg ctacttcaat tactcaggat tggaaaaaac ttgatattat gatcgcaaat gcaggaatcc ttggaactct ttcaccaata gctcacatag aaaataaggt atttgaagat atttttcaaa taaatcttat cagccaatgg cgcttaatga aagctgttga acccttattg cgtaaatctg atgctggacg agtaattctt ttatcttcaa gcgttgctca tgttgcccgc gctttctggg gaccctatgc agcctctaaa gcggctctag aaattatttc ccgttgttgg gcagaagaat taaaacaaac cccaatcaag attaattgtg ttgatcccgg tgcaacacgc actgccatgc gcgcccaagc tatgcctggg gaagatcctc aaactcttcc tccaccacaa gaagttgcag aaaagatagc acatttactc tcacctgatt taaaggaaac cggcaaactc tttaatgtgc gtaaaaatcg attcatgaat tatcacgcac ctaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa