BaheA.00720.b

TrwD protein

CENTER ID:	BaheA.00720.b
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00720.b.A1.GE31171	Full length( BaheA.00720.b )		1	353

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00720.b.A1.PS01365	Full length( BaheA.00720.b )		1	353

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130713
PATRIC ID:	fig\|283166.5.peg.1830
RefSeq:	YP_034272.1
UniProt:	A0A0H3LZX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVSLHVNP IQKDQAVAQL LQPLDPFLKD PKIIELSIGH PCEVWTKSLE GWQLHSVPEL TSSFLQTLIT AIIVYNGMVP KSINYVRLPG GQRGTIVQTP AVIEGTLSFM IRKHSLTVKT LEELNEENAF NHFTDVSFNK PSEKEANDLL SKQDFTRLEP FEIELLQFKR ERKILEFLEK CVLYKRNIII AGKTGSGKTT FARSLIEKVP VEERIITIED VHELFLPNHP NHVHMIYGDN MGRVSAEECL DACMRQSPDR IFLAELRGNE AWEYLNSLNT GHPGSITTTH ANNALQTFER CATLIKRSEV GRLLDIEMIR MVLYSTIDVV LFFKDRKLSE IFYDPIFSKS KIA

NT Sequence

ATGTCTACAG TCTCGTTACA CGTTAACCCC ATACAAAAAG ATCAAGCAGT TGCACAGCTT CTACAACCGC TTGATCCTTT CTTAAAAGAT CCTAAAATTA TTGAATTATC GATTGGTCAC CCCTGTGAAG TATGGACAAA AAGTTTAGAG GGTTGGCAGC TACATAGTGT ACCAGAGCTG ACAAGCTCTT TTTTGCAAAC GCTTATTACA GCCATTATTG TTTATAATGG GATGGTTCCG AAAAGCATCA ATTATGTGCG CTTACCCGGC GGACAACGTG GTACAATTGT GCAAACACCA GCTGTCATCG AAGGTACTTT ATCCTTTATG ATTCGGAAGC ATTCCCTCAC AGTGAAGACA CTAGAAGAAC TCAACGAAGA AAATGCATTC AATCACTTTA CTGACGTAAG TTTCAATAAA CCTTCAGAAA AAGAAGCGAA TGACTTACTA TCGAAACAGG ATTTTACACG ATTGGAGCCA TTTGAAATCG AACTTTTGCA ATTTAAACGT GAGAGAAAAA TTCTTGAATT TTTGGAAAAA TGCGTACTGT ATAAACGCAA CATCATTATT GCAGGAAAAA CAGGATCAGG TAAAACAACA TTTGCTCGTT CTTTAATCGA AAAGGTGCCT GTAGAAGAGC GTATTATTAC AATTGAAGAT GTTCATGAGC TTTTTTTACC AAATCATCCC AACCATGTGC ATATGATTTA TGGTGACAAT ATGGGTCGTG TTTCCGCTGA AGAATGTTTA GATGCGTGTA TGCGTCAATC GCCTGACCGT ATTTTTCTCG CTGAGCTACG TGGTAATGAG GCCTGGGAAT ATCTCAATTC ATTGAACACC GGTCATCCTG GATCAATTAC CACAACACAT GCGAACAATG CTTTGCAAAC ATTTGAACGT TGTGCCACAT TAATCAAAAG ATCAGAAGTT GGCCGTCTAC TCGATATAGA GATGATAAGG ATGGTACTCT ACAGCACAAT TGATGTGGTG TTGTTCTTTA AGGATAGAAA GCTTTCTGAA ATTTTTTACG ACCCGATTTT TTCTAAATCA AAGATAGCT

Details for BaheA.00720.b.A1.GE31171

HARVESTED ON:	1/11/2011
SEQUENCED ON:	1/18/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

HHHMGTLEAQ TQGPGSMSTV SLHVNPIQKD QAVAQLLQPL DPFLKDPKII ELSIGHPCEV WTKSLEGWQL HSVPELTSSF LQTLITAIIV YNGMVPKSIN YVRLPGGQRG TIVQTPAVIE GTLSFMIRKH SLTVKTLEEL NEENAFNHFT DVSFNKPSEK EANDLLSKQD FTRLEPFEIE LLQFKRERKI LEFLEKCVLY KRNIIIAGKT GSGKTTFARS LIEKVPVEER IITIEDVHEL FLPNHPNHVH MIYGDNMGRV SAEECLDACM RQSPDRIFLA ELRGNEAWEY LNSLNTGHPG SITTTHANNA LQTFERCATL IKRSEVGRLL DIEMIRMVLY STIDVVLFFK DRKLSEIFYD PIFSKSKIA

Validated NT Sequence

tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgtctacagt ctcgttacac gttaacccca tacaaaaaga tcaagcagtt gcacagcttc tacaaccgct tgatcctttc ttaaaagatc ctaaaattat tgaattatcg attggtcacc cctgtgaagt atggacaaaa agtttagagg gttggcagct acatagtgta ccagagctga caagctcttt tttgcaaacg cttattacag ccattattgt ttataatggg atggttccga aaagcatcaa ttatgtgcgc ttacccggcg gacaacgtgg tacaattgtg caaacaccag ctgtcatcg- aaggtacctt atcctttatg attcggaagc attccctcac agtgaagaca ctagaagaac tcaacgaaga aaatgcattc aatcacttta ctgacgtaag tttcaataaa ccttcagaaa aagaagcgaa tgacttacta tcgaaacagg attttacacg attggagcca tttgaaatcg aacttttgca atttaaacgt gagagaaaaa ttcttgaatt tttggaaaaa tgcgtactgt ataaacgcaa catcattatt gcaggaaaaa caggatcagg taaaacaaca tttgctcgtt ctttaatcga aaa-ggtgcc tgtagaagag cgtattatta caattgaaga tgttcatgag cttttttt-a ccaaatcatc ccaaccatgt gcatatgatt tatggtgaca atatgggtcg tg-tttccgc tgaagaatgt ttagatgcgt gtatgcgtca atcgcctgac cgtatttttc tcgctgagct acgtggtaat gaggcctggg aatatctcaa ttcattgaac accggtcatc ctggatcaat taccacaaca catgcgaaca atgctttgca aacatttgaa cgttgtgcca cattaatcaa aagatcagaa gttggccgtc tactcgatat agagatgata aggatggtac tctacagcac aattgatgtg gtgttgttct ttaaggatag aaagctttct gaaatttttt acgacccgat tttttctaaa tcaaagatag cttaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTVSLHVN PIQKDQAVAQ LLQPLDPFLK DPKIIELSIG HPCEVWTKSL EGWQLHSVPE LTSSFLQTLI TAIIVYNGMV PKSINYVRLP GGQRGTIVQT PAVIEGTLSF MIRKHSLTVK TLEELNEENA FNHFTDVSFN KPSEKEANDL LSKQDFTRLE PFEIELLQFK RERKILEFLE KCVLYKRNII IAGKTGSGKT TFARSLIEKV PVEERIITIE DVHELFLPNH PNHVHMIYGD NMGRVSAEEC LDACMRQSPD RIFLAELRGN EAWEYLNSLN TGHPGSITTT HANNALQTFE RCATLIKRSE VGRLLDIEMI RMVLYSTIDV VLFFKDRKLS EIFYDPIFSK SKIA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctacagtc tcgttacacg ttaaccccat acaaaaagat caagcagttg cacagcttct acaaccgctt gatcctttct taaaagatcc taaaattatt gaattatcga ttggtcaccc ctgtgaagta tggacaaaaa gtttagaggg ttggcagcta catagtgtac cagagctgac aagctctttt ttgcaaacgc ttattacagc cattattgtt tataatggga tggttccgaa aagcatcaat tatgtgcgct tacccggcgg acaacgtggt acaattgtgc aaacaccagc tgtcatcgaa ggtactttat cctttatgat tcggaagcat tccctcacag tgaagacact agaagaactc aacgaagaaa atgcattcaa tcactttact gacgtaagtt tcaataaacc ttcagaaaaa gaagcgaatg acttactatc gaaacaggat tttacacgat tggagccatt tgaaatcgaa cttttgcaat ttaaacgtga gagaaaaatt cttgaatttt tggaaaaatg cgtactgtat aaacgcaaca tcattattgc aggaaaaaca ggatcaggta aaacaacatt tgctcgttct ttaatcgaaa aggtgcctgt agaagagcgt attattacaa ttgaagatgt tcatgagctt tttttaccaa atcatcccaa ccatgtgcat atgatttatg gtgacaatat gggtcgtgtt tccgctgaag aatgtttaga tgcgtgtatg cgtcaatcgc ctgaccgtat ttttctcgct gagctacgtg gtaatgaggc ctgggaatat ctcaattcat tgaacaccgg tcatcctgga tcaattacca caacacatgc gaacaatgct ttgcaaacat ttgaacgttg tgccacatta atcaaaagat cagaagttgg ccgtctactc gatatagaga tgataaggat ggtactctac agcacaattg atgtggtgtt gttctttaag gatagaaagc tttctgaaat tttttacgac ccgatttttt ctaaatcaaa gatagcttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BaheA.00720.b.A1.PS01365

PURIFICATION DATe:	4/13/2011
CONCENTRATION:	16.74mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTACAGTC TCGTTACACG TTAACCCCAT ACAAAAAGAT CAAGCAGTTG CACAGCTTCT ACAACCGCTT GATCCTTTCT TAAAAGATCC TAAAATTATT GAATTATCGA TTGGTCACCC CTGTGAAGTA TGGACAAAAA GTTTAGAGGG TTGGCAGCTA CATAGTGTAC CAGAGCTGAC AAGCTCTTTT TTGCAAACGC TTATTACAGC CATTATTGTT TATAATGGGA TGGTTCCGAA AAGCATCAAT TATGTGCGCT TACCCGGCGG ACAACGTGGT ACAATTGTGC AAACACCAGC TGTCATCGAA GGTACTTTAT CCTTTATGAT TCGGAAGCAT TCCCTCACAG TGAAGACACT AGAAGAACTC AACGAAGAAA ATGCATTCAA TCACTTTACT GACGTAAGTT TCAATAAACC TTCAGAAAAA GAAGCGAATG ACTTACTATC GAAACAGGAT TTTACACGAT TGGAGCCATT TGAAATCGAA CTTTTGCAAT TTAAACGTGA GAGAAAAATT CTTGAATTTT TGGAAAAATG CGTACTGTAT AAACGCAACA TCATTATTGC AGGAAAAACA GGATCAGGTA AAACAACATT TGCTCGTTCT TTAATCGAAA AGGTGCCTGT AGAAGAGCGT ATTATTACAA TTGAAGATGT TCATGAGCTT TTTTTACCAA ATCATCCCAA CCATGTGCAT ATGATTTATG GTGACAATAT GGGTCGTGTT TCCGCTGAAG AATGTTTAGA TGCGTGTATG CGTCAATCGC CTGACCGTAT TTTTCTCGCT GAGCTACGTG GTAATGAGGC CTGGGAATAT CTCAATTCAT TGAACACCGG TCATCCTGGA TCAATTACCA CAACACATGC GAACAATGCT TTGCAAACAT TTGAACGTTG TGCCACATTA ATCAAAAGAT CAGAAGTTGG CCGTCTACTC GATATAGAGA TGATAAGGAT GGTACTCTAC AGCACAATTG ATGTGGTGTT GTTCTTTAAG GATAGAAAGC TTTCTGAAAT TTTTTACGAC CCGATTTTTT CTAAATCAAA GATAGCTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA