BaheA.00720.a

virB11 protein homolog (VirB type IV secretion machinery)

CENTER ID:	BaheA.00720.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00720.a.B1.GE27109	Full length( BaheA.00720.a )		1	356

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130713
PATRIC ID:	fig\|283166.5.peg.1455
RefSeq:	YP_034060.1
UniProt:	Q9RNC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQNLHTLSD ETVAIVLTKL EPISTFLKDE NLFEIVINRP YQVMTEGVEG WKTIETPALS FNELMGIAKV VASYSKQNIS EKNPILSATL PGNERIQIVI PPAVEKNTIS MTIRKPSSRS FSLEDLANKG LFSVCEQVSF TPLNNYLSHL SELKHIDHDL VRAYAKKDFV FFLNQAVQCQ KNILIAGKTG SGKTTLSKAL IAKIPDDERI ITIEDTPELV VPQPNYVSMI YSKDGQGLAS VGPKELLESA LRMRPDRILL QELRDGTAFY YIRNVNSGHP GSITTVHAST ALAAFEQMTL LVKESEGGGD LERDDIRGLL ISMIDIIIQC KRIEGKFKVT EIYYDPFKQR NIFGGN

NT Sequence

ATGAACCAAA ACTTGCACAC ATTGAGCGAT GAAACCGTCG CGATTGTTTT AACAAAACTA GAACCTATCA GTACCTTTCT AAAAGATGAA AATCTTTTTG AAATTGTCAT CAATCGTCCC TATCAAGTAA TGACAGAAGG GGTTGAGGGA TGGAAAACCA TAGAAACACC AGCTCTGTCA TTTAATGAGC TTATGGGAAT TGCTAAAGTT GTAGCGTCTT ATTCTAAGCA AAATATATCG GAAAAAAATC CAATATTATC AGCCACCTTA CCAGGCAATG AGCGTATTCA AATTGTTATT CCCCCTGCGG TAGAAAAAAA CACCATCAGT ATGACAATTC GCAAACCCTC ATCACGGAGT TTTTCCCTCG AAGATCTGGC AAATAAAGGG CTCTTTTCGG TGTGTGAACA AGTTTCCTTC ACGCCCTTGA ATAATTATCT CTCACATTTG AGTGAACTCA AACATATTGA TCATGACTTA GTCCGTGCTT ATGCAAAGAA GGATTTTGTC TTCTTTTTAA ATCAGGCTGT ACAATGCCAA AAAAACATTT TGATTGCCGG AAAAACCGGC TCGGGTAAAA CAACGCTTTC AAAGGCATTA ATCGCCAAAA TTCCCGATGA TGAACGTATT ATCACCATTG AAGATACACC AGAATTGGTC GTTCCACAGC CTAATTATGT TTCTATGATC TATTCAAAAG ATGGTCAAGG TTTAGCCTCT GTTGGTCCAA AAGAACTGCT TGAATCTGCT TTACGCATGC GTCCTGACCG CATTCTTTTA CAAGAGCTAC GAGATGGTAC CGCCTTTTAT TATATCCGCA ATGTTAATTC AGGACATCCA GGTTCAATTA CCACGGTTCA TGCCTCAACA GCACTTGCTG CCTTTGAGCA AATGACCCTT TTGGTGAAAG AAAGTGAGGG TGGAGGAGAC TTAGAGCGTG ACGATATTCG AGGGCTGTTG ATTTCAATGA TTGATATCAT CATTCAATGT AAACGGATTG AGGGAAAATT TAAGGTCACA GAAATTTATT ATGATCCCTT TAAACAAAGA AACATCTTTG GTGGGAAT

Details for BaheA.00720.a.B1.GE27109

HARVESTED ON:	8/17/2009
SEQUENCED ON:	9/29/2009
EXPECTED MW:	40kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	84

Validated AA Sequence

MTEGVEGWKT IETPALSFNE LMGIAKVVAS YSKQNISEKN PILSATLPGN ERIQIVIPPA VEKNTISMTI RKPSSRSFSL EDLANKGLFS VCEQVSFTPL NNYLSHLSEL KHIDHDLVRA YAKKDFVFFL NQAVQCQKNI LIAGKTGSGK TTLSKALIAK IPDDERIITI EDTPELVVPQ PNYVSMIYSK DGQGLASVGP KELLESALRM RPDRILLQEL RDGTTFYYIR NVNSGHPGSI TTVHASTALA AFEQMTLLVK ESEGGGDLER DDIRGLLISM IDIIIQCKRI EGKFKVTEIY YDPFKQ

Validated NT Sequence

tttgtttaaa gggatcataa taaatttctg tgaccttaaa ttttccctca atccgtttac attgaatgat gatatcaatc attgaaatca acagccctcg aatatcgtca cgctctaagt ctcctccacc ctcactttct ttcaccaaaa gggtcatttg ctcaaaggca gcaagtgctg ttgaggcatg aaccgtggta attgaacctg gatgtcctga attaacattg cggatataat aaaaggtggt accatctcgt agctcttgta aaagaatgcg gtcaggacgc atgcgtaaag cagattcaag cagttctttt ggaccaacag aggctaaacc ttgaccatct tttgaataga tcatagaaac ataattaggc tgtggaacga ccaattctgg tgtatcttca atggtgataa tacgttcatc atcgggaatt ttggcgatta atgcctttga aagcgttgtt ttacccgagc cggtttttcc ggcaatcaaa atgttttttt ggcattgtac agcctgattt aaaaagaaga caaaatcctt ctttgcataa gcacggacta agtcatgatc aatatgtttg agttcactca aatgtgagag ataattattc aagggcgtga aggaaacttg ttcacacacc gaaaagagcc ctttatttgc cagatcttcg agggaaaaac tccgtgatga gggtttgcga attgtcatac tgattgtgtt tttttctacc gcagggggaa taacaatttg aatacgctca ttgcctggta aggtggctga taatattgga tttttttccg atatattttg cttagaataa gacgctacaa ctttagcaat tcccataagc tcattaaatg acagagctgg tgtttctatg gttttccatc cctcaacccc ttctgtcatt acttgatagg gacgattgat gacaatttca aaaagatttt catcttttag aaaggtactg ataggttcta gttttgttaa aacaatcgcg aaggtttcat cgctcaatgt gtgcaagttt tgg

Expected Protein Sequence

MAHHHHHHMN QNLHTLSDET VAIVLTKLEP ISTFLKDENL FEIVINRPYQ VMTEGVEGWK TIETPALSFN ELMGIAKVVA SYSKQNISEK NPILSATLPG NERIQIVIPP AVEKNTISMT IRKPSSRSFS LEDLANKGLF SVCEQVSFTP LNNYLSHLSE LKHIDHDLVR AYAKKDFVFF LNQAVQCQKN ILIAGKTGSG KTTLSKALIA KIPDDERIIT IEDTPELVVP QPNYVSMIYS KDGQGLASVG PKELLESALR MRPDRILLQE LRDGTAFYYI RNVNSGHPGS ITTVHASTAL AAFEQMTLLV KESEGGGDLE RDDIRGLLIS MIDIIIQCKR IEGKFKVTEI YYDPFKQRNI FGGN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc aaaacttgca cacattgagc gatgaaaccg tcgcgattgt tttaacaaaa ctagaaccta tcagtacctt tctaaaagat gaaaatcttt ttgaaattgt catcaatcgt ccctatcaag taatgacaga aggggttgag ggatggaaaa ccatagaaac accagctctg tcatttaatg agcttatggg aattgctaaa gttgtagcgt cttattctaa gcaaaatata tcggaaaaaa atccaatatt atcagccacc ttaccaggca atgagcgtat tcaaattgtt attccccctg cggtagaaaa aaacaccatc agtatgacaa ttcgcaaacc ctcatcacgg agtttttccc tcgaagatct ggcaaataaa gggctctttt cggtgtgtga acaagtttcc ttcacgccct tgaataatta tctctcacat ttgagtgaac tcaaacatat tgatcatgac ttagtccgtg cttatgcaaa gaaggatttt gtcttctttt taaatcaggc tgtacaatgc caaaaaaaca ttttgattgc cggaaaaacc ggctcgggta aaacaacgct ttcaaaggca ttaatcgcca aaattcccga tgatgaacgt attatcacca ttgaagatac accagaattg gtcgttccac agcctaatta tgtttctatg atctattcaa aagatggtca aggtttagcc tctgttggtc caaaagaact gcttgaatct gctttacgca tgcgtcctga ccgcattctt ttacaagagc tacgagatgg taccgccttt tattatatcc gcaatgttaa ttcaggacat ccaggttcaa ttaccacggt tcatgcctca acagcacttg ctgcctttga gcaaatgacc cttttggtga aagaaagtga gggtggagga gacttagagc gtgacgatat tcgagggctg ttgatttcaa tgattgatat catcattcaa tgtaaacgga ttgagggaaa atttaaggtc acagaaattt attatgatcc ctttaaacaa agaaacatct ttggtgggaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat