BaheA.00711.a

Malate dehydrogenase

CENTER ID: BaheA.00711.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00711.a.A1.GE25802 Full length( BaheA.00711.a ) 1 320
BaheA.00711.a.AE1.GE43124 Full length( BaheA.00711.a ) 1 320
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00711.a.A1.PS00118 Full length( BaheA.00711.a ) 1 320
BaheA.00711.a.A1.PS00355 Full length( BaheA.00711.a ) 1 320
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126911
PATRIC ID: fig|283166.5.peg.1927
RefSeq: YP_034347.1
UniProt: Q6G1M0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARKKIALIG SGMIGGTLAH IIGLKELGDV VLFDIAEGIP QGKALDIAES SPVDGFDVSL TGANSYDVIE GADVVIVTAG VARKPGMSRD DLLGINLKVM EQVGAGIKKY ASSAFVICIT NPLDAMVWAL QKFSGLPTQK VVGMAGILDS ARFRHFLSEE FKISVKDVTA FVLGGHGDSM VPLVRYSTVG GISLPDLVKM GWTTQEKIDQ IIQRTRDGGA EIVSLLKTGS AFYAPAASAV SMAEAYLKDT KRVVPVAAYL SGQYGVKDTY VGVPVVIGAG GVERVIEIDL DKEEKAAFEK SVSAVQKLCE ACIAVAPGLK
NT Sequence
ATGGCACGAA AGAAAATAGC TCTTATTGGT TCGGGTATGA TTGGGGGTAC TTTAGCACAT ATAATTGGAC TTAAAGAACT TGGTGATGTC GTCTTATTTG ATATTGCAGA AGGTATACCA CAAGGTAAAG CTCTTGATAT CGCCGAATCT TCACCAGTTG ACGGTTTTGA TGTCAGTTTA ACCGGTGCTA ATTCCTATGA TGTGATTGAA GGTGCCGATG TTGTTATTGT AACAGCAGGT GTTGCAAGAA AACCCGGTAT GAGCCGTGAT GACCTTTTGG GCATTAATTT AAAGGTAATG GAACAAGTTG GTGCGGGGAT TAAAAAATAT GCATCTTCAG CATTCGTTAT TTGTATTACA AATCCTCTTG ATGCAATGGT CTGGGCTTTG CAAAAATTTT CAGGTCTTCC AACACAAAAA GTGGTTGGTA TGGCTGGTAT TCTCGATTCA GCACGTTTTC GTCATTTCTT ATCAGAGGAA TTTAAGATCT CTGTTAAAGA TGTGACTGCA TTCGTTTTAG GAGGACACGG TGATTCAATG GTGCCTTTGG TGCGTTATTC AACAGTTGGT GGTATTTCTC TACCCGATCT TGTGAAAATG GGCTGGACGA CGCAAGAAAA GATTGATCAA ATTATTCAAC GTACCCGTGA TGGTGGTGCA GAAATTGTTA GTTTGTTAAA AACAGGTTCT GCTTTTTATG CACCAGCAGC TTCTGCTGTC TCTATGGCTG AAGCCTATTT GAAAGACACC AAGCGTGTTG TTCCTGTCGC AGCTTATCTT TCAGGGCAAT ATGGGGTCAA AGATACCTAT GTTGGTGTTC CGGTTGTCAT TGGTGCTGGG GGCGTTGAGC GCGTGATTGA AATCGATCTT GATAAAGAGG AGAAAGCTGC TTTTGAAAAA TCAGTGAGTG CAGTGCAAAA ACTTTGTGAA GCTTGTATTG CTGTTGCACC AGGACTCAAA
Details for BaheA.00711.a.A1.GE25802
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 35kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARKKIALI GSGMIGGTLA HIIGLKELGD VVLFDIAEGI PQGKALDIAE SSPVDGFDVS LTGANSYDVI EGADVVIVTA GVARKPGMSR DDLLGINLKV MEQVGAGIKK YASSAFVICI TNPLDAMVWA LQKFSGLPTQ KVVGMAGILD SARFRHFLSE EFKISVKDVT AFVLGGHGDS MVPLVRYSTV GGISLPDLVK MGWTTQEKID QIIQRTRDGG AEIVSLLKTG SAFYAPAASA VSMAEAYLKD TKRVVPVAAY LSGQYGVKDT YVGVPVVIGA GGVERVIEID LDKEEKAAFE KSVSAVQKLC EACIAVAPGL K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gaaagaaaat agctcttatt ggttcgggta tgattggggg tactttagca catataattg gacttaaaga acttggtgat gtcgtcttat ttgatattgc agaaggtata ccacaaggta aagctcttga tatcgccgaa tcttcaccag ttgacggttt tgatgtcagt ttaaccggtg ctaattccta tgatgtgatt gaaggtgccg atgttgttat tgtaacagca ggtgttgcaa gaaaacccgg tatgagccgt gatgaccttt tgggcattaa tttaaaggta atggaacaag ttggtgcggg gattaaaaaa tatgcatctt cagcattcgt tatttgtatt acaaatcctc ttgatgcaat ggtctgggct ttgcaaaaat tttcaggtct tccaacacaa aaagtggttg gtatggctgg tattctcgat tcagcacgtt ttcgtcattt cttatcagag gaatttaaga tctctgttaa agatgtgact gcattcgttt taggaggaca cggtgattca atggtgcctt tggtgcgtta ttcaacagtt ggtggtattt ctctacccga tcttgtgaaa atgggctgga cgacgcaaga aaagattgat caaattattc aacgtacccg tgatggtggt gcagaaattg ttagtttgtt aaaaacaggt tctgcttttt atgcaccagc agcttctgct gtctctatgg ctgaagccta tttgaaagac accaagcgtg ttgttcctgt cgcagcttat ctttcagggc aatatggggt caaagatacc tatgttggtg ttccggttgt cattggtgct gggggcgttg agcgcgtgat tgaaatcgat cttgataaag aggagaaagc tgcttttgaa aaatcagtga gtgcagtgca aaaactttgt gaagcttgta ttgctgttgc accaggactc aaataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARKKIALI GSGMIGGTLA HIIGLKELGD VVLFDIAEGI PQGKALDIAE SSPVDGFDVS LTGANSYDVI EGADVVIVTA GVARKPGMSR DDLLGINLKV MEQVGAGIKK YASSAFVICI TNPLDAMVWA LQKFSGLPTQ KVVGMAGILD SARFRHFLSE EFKISVKDVT AFVLGGHGDS MVPLVRYSTV GGISLPDLVK MGWTTQEKID QIIQRTRDGG AEIVSLLKTG SAFYAPAASA VSMAEAYLKD TKRVVPVAAY LSGQYGVKDT YVGVPVVIGA GGVERVIEID LDKEEKAAFE KSVSAVQKLC EACIAVAPGL K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgaaag aaaatagctc ttattggttc gggtatgatt gggggtactt tagcacatat aattggactt aaagaacttg gtgatgtcgt cttatttgat attgcagaag gtataccaca aggtaaagct cttgatatcg ccgaatcttc accagttgac ggttttgatg tcagtttaac cggtgctaat tcctatgatg tgattgaagg tgccgatgtt gttattgtaa cagcaggtgt tgcaagaaaa cccggtatga gccgtgatga ccttttgggc attaatttaa aggtaatgga acaagttggt gcggggatta aaaaatatgc atcttcagca ttcgttattt gtattacaaa tcctcttgat gcaatggtct gggctttgca aaaattttca ggtcttccaa cacaaaaagt ggttggtatg gctggtattc tcgattcagc acgttttcgt catttcttat cagaggaatt taagatctct gttaaagatg tgactgcatt cgttttagga ggacacggtg attcaatggt gcctttggtg cgttattcaa cagttggtgg tatttctcta cccgatcttg tgaaaatggg ctggacgacg caagaaaaga ttgatcaaat tattcaacgt acccgtgatg gtggtgcaga aattgttagt ttgttaaaaa caggttctgc tttttatgca ccagcagctt ctgctgtctc tatggctgaa gcctatttga aagacaccaa gcgtgttgtt cctgtcgcag cttatctttc agggcaatat ggggtcaaag atacctatgt tggtgttccg gttgtcattg gtgctggggg cgttgagcgc gtgattgaaa tcgatcttga taaagaggag aaagctgctt ttgaaaaatc agtgagtgca gtgcaaaaac tttgtgaagc ttgtattgct gttgcaccag gactcaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BaheA.00711.a.AE1.GE43124
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MARKKIALIG SGMIGGTLAH IIGLKELGDV VLFDIAEGIP QGKALDIAES SPVDGFDVSL TGANSYDVIE GADVVIVTAG VARKPGMSRD DLLGINLKVM EQVGAGIKKY ASSAFVICIT NPLDAMVWAL QKFSGLPTQK VVGMAGILDS ARFRHFLSEE FKISVKDVTA FVLGGHGDSM VPLVRYSTVG GISLPDLVKM GWTTQEKIDQ IIQRTRDGGA EIVSLLKTGS AFYAPAASAV SMAEAYLKDT KRVVPVAAYL SGQYGVKDTY VGVPVVIGAG GVERVIEIDL DKEEKAAFEK SVSAVQKLCE ACIAVAPGLK AGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctttgagtcc tggtgcaaca gcaatacaag cttcacaaag tttttgcact gcactcactg atttttcaaa agcagctttc tcctctttat caagatcgat ttcaatcacg cgctcaacgc ccccagcacc aatgacaacc ggaacaccaa cataggtatc tttgacccca tattgccctg aaagataagc tgcgacagga acaacacgct tggtgtcttt caaataggct tcagccatag agacagcaga agctgctggt gcataaaaag cagaacctgt ttttaacaaa ctaacaattt ctgcaccacc atcacgggta cgttgaataa tttgatcaat cttttcttgc gtcgtccagc ccattttcac aagatcgggt agagaaatac caccaactgt tgaataacgc accaaaggca ccattgaatc accgtgtcct cctaaaacga atgcagtcac atctttaaca gagatcttaa attcctctga taagaaatga cgaaaacgtg ctgaatcgag aataccagcc ataccaacca ctttttgtgt tggaagacct gaaaattttt gcaaagccca gaccattgca tcaagaggat ttgtaataca aataacgaat gctgaagatg catatttttt aatccccgca ccaacttgtt ccattacctt taaattaatg cccaaaaggt catcacggct cataccgggt tttcttgcaa cacctgctgt tacaataaca acatcggcac cttcaatcac atcataggaa ttagcaccgg ttaaactgac atcaaaaccg tcaactggtg aagattcggc gatatcaaga gctttacctt gtggtatacc ttctgcaata tcaaataaga cgacatcacc aagttcttta agtccaatta tatgtgctaa agtaccccca atcatacccg aaccaataag agctattttc tttcgtgcca tgggagaga
Expected Protein Sequence
MARKKIALIG SGMIGGTLAH IIGLKELGDV VLFDIAEGIP QGKALDIAES SPVDGFDVSL TGANSYDVIE GADVVIVTAG VARKPGMSRD DLLGINLKVM EQVGAGIKKY ASSAFVICIT NPLDAMVWAL QKFSGLPTQK VVGMAGILDS ARFRHFLSEE FKISVKDVTA FVLGGHGDSM VPLVRYSTVG GISLPDLVKM GWTTQEKIDQ IIQRTRDGGA EIVSLLKTGS AFYAPAASAV SMAEAYLKDT KRVVPVAAYL SGQYGVKDTY VGVPVVIGAG GVERVIEIDL DKEEKAAFEK SVSAVQKLCE ACIAVAPGLK GHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggcacgaaag aaaatagctc ttattggttc gggtatgatt gggggtactt tagcacatat aattggactt aaagaacttg gtgatgtcgt cttatttgat attgcagaag gtataccaca aggtaaagct cttgatatcg ccgaatcttc accagttgac ggttttgatg tcagtttaac cggtgctaat tcctatgatg tgattgaagg tgccgatgtt gttattgtaa cagcaggtgt tgcaagaaaa cccggtatga gccgtgatga ccttttgggc attaatttaa aggtaatgga acaagttggt gcggggatta aaaaatatgc atcttcagca ttcgttattt gtattacaaa tcctcttgat gcaatggtct gggctttgca aaaattttca ggtcttccaa cacaaaaagt ggttggtatg gctggtattc tcgattcagc acgttttcgt catttcttat cagaggaatt taagatctct gttaaagatg tgactgcatt cgttttagga ggacacggtg attcaatggt gcctttggtg cgttattcaa cagttggtgg tatttctcta cccgatcttg tgaaaatggg ctggacgacg caagaaaaga ttgatcaaat tattcaacgt acccgtgatg gtggtgcaga aattgttagt ttgttaaaaa caggttctgc tttttatgca ccagcagctt ctgctgtctc tatggctgaa gcctatttga aagacaccaa gcgtgttgtt cctgtcgcag cttatctttc agggcaatat ggggtcaaag atacctatgt tggtgttccg gttgtcattg gtgctggggg cgttgagcgc gtgattgaaa tcgatcttga taaagaggag aaagctgctt ttgaaaaatc agtgagtgca gtgcaaaaac tttgtgaagc ttgtattgct gttgcaccag gactcaaagg gcaccaccat catcatcatt aacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat
Details for BaheA.00711.a.A1.PS00118
PURIFICATION DATe: 1/14/2009
CONCENTRATION: 69.73mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARKKIALI GSGMIGGTLA HIIGLKELGD VVLFDIAEGI PQGKALDIAE SSPVDGFDVS LTGANSYDVI EGADVVIVTA GVARKPGMSR DDLLGINLKV MEQVGAGIKK YASSAFVICI TNPLDAMVWA LQKFSGLPTQ KVVGMAGILD SARFRHFLSE EFKISVKDVT AFVLGGHGDS MVPLVRYSTV GGISLPDLVK MGWTTQEKID QIIQRTRDGG AEIVSLLKTG SAFYAPAASA VSMAEAYLKD TKRVVPVAAY LSGQYGVKDT YVGVPVVIGA GGVERVIEID LDKEEKAAFE KSVSAVQKLC EACIAVAPGL K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gaaagaaaat agctcttatt ggttcgggta tgattggggg tactttagca catataattg gacttaaaga acttggtgat gtcgtcttat ttgatattgc agaaggtata ccacaaggta aagctcttga tatcgccgaa tcttcaccag ttgacggttt tgatgtcagt ttaaccggtg ctaattccta tgatgtgatt gaaggtgccg atgttgttat tgtaacagca ggtgttgcaa gaaaacccgg tatgagccgt gatgaccttt tgggcattaa tttaaaggta atggaacaag ttggtgcggg gattaaaaaa tatgcatctt cagcattcgt tatttgtatt acaaatcctc ttgatgcaat ggtctgggct ttgcaaaaat tttcaggtct tccaacacaa aaagtggttg gtatggctgg tattctcgat tcagcacgtt ttcgtcattt cttatcagag gaatttaaga tctctgttaa agatgtgact gcattcgttt taggaggaca cggtgattca atggtgcctt tggtgcgtta ttcaacagtt ggtggtattt ctctacccga tcttgtgaaa atgggctgga cgacgcaaga aaagattgat caaattattc aacgtacccg tgatggtggt gcagaaattg ttagtttgtt aaaaacaggt tctgcttttt atgcaccagc agcttctgct gtctctatgg ctgaagccta tttgaaagac accaagcgtg ttgttcctgt cgcagcttat ctttcagggc aatatggggt caaagatacc tatgttggtg ttccggttgt cattggtgct gggggcgttg agcgcgtgat tgaaatcgat cttgataaag aggagaaagc tgcttttgaa aaatcagtga gtgcagtgca aaaactttgt gaagcttgta ttgctgttgc accaggactc aaataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARKKIALI GSGMIGGTLA HIIGLKELGD VVLFDIAEGI PQGKALDIAE SSPVDGFDVS LTGANSYDVI EGADVVIVTA GVARKPGMSR DDLLGINLKV MEQVGAGIKK YASSAFVICI TNPLDAMVWA LQKFSGLPTQ KVVGMAGILD SARFRHFLSE EFKISVKDVT AFVLGGHGDS MVPLVRYSTV GGISLPDLVK MGWTTQEKID QIIQRTRDGG AEIVSLLKTG SAFYAPAASA VSMAEAYLKD TKRVVPVAAY LSGQYGVKDT YVGVPVVIGA GGVERVIEID LDKEEKAAFE KSVSAVQKLC EACIAVAPGL K
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACGAAAG AAAATAGCTC TTATTGGTTC GGGTATGATT GGGGGTACTT TAGCACATAT AATTGGACTT AAAGAACTTG GTGATGTCGT CTTATTTGAT ATTGCAGAAG GTATACCACA AGGTAAAGCT CTTGATATCG CCGAATCTTC ACCAGTTGAC GGTTTTGATG TCAGTTTAAC CGGTGCTAAT TCCTATGATG TGATTGAAGG TGCCGATGTT GTTATTGTAA CAGCAGGTGT TGCAAGAAAA CCCGGTATGA GCCGTGATGA CCTTTTGGGC ATTAATTTAA AGGTAATGGA ACAAGTTGGT GCGGGGATTA AAAAATATGC ATCTTCAGCA TTCGTTATTT GTATTACAAA TCCTCTTGAT GCAATGGTCT GGGCTTTGCA AAAATTTTCA GGTCTTCCAA CACAAAAAGT GGTTGGTATG GCTGGTATTC TCGATTCAGC ACGTTTTCGT CATTTCTTAT CAGAGGAATT TAAGATCTCT GTTAAAGATG TGACTGCATT CGTTTTAGGA GGACACGGTG ATTCAATGGT GCCTTTGGTG CGTTATTCAA CAGTTGGTGG TATTTCTCTA CCCGATCTTG TGAAAATGGG CTGGACGACG CAAGAAAAGA TTGATCAAAT TATTCAACGT ACCCGTGATG GTGGTGCAGA AATTGTTAGT TTGTTAAAAA CAGGTTCTGC TTTTTATGCA CCAGCAGCTT CTGCTGTCTC TATGGCTGAA GCCTATTTGA AAGACACCAA GCGTGTTGTT CCTGTCGCAG CTTATCTTTC AGGGCAATAT GGGGTCAAAG ATACCTATGT TGGTGTTCCG GTTGTCATTG GTGCTGGGGG CGTTGAGCGC GTGATTGAAA TCGATCTTGA TAAAGAGGAG AAAGCTGCTT TTGAAAAATC AGTGAGTGCA GTGCAAAAAC TTTGTGAAGC TTGTATTGCT GTTGCACCAG GACTCAAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BaheA.00711.a.A1.PS00355
PURIFICATION DATe: 9/18/2009
CONCENTRATION: 41.89mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARKKIALI GSGMIGGTLA HIIGLKELGD VVLFDIAEGI PQGKALDIAE SSPVDGFDVS LTGANSYDVI EGADVVIVTA GVARKPGMSR DDLLGINLKV MEQVGAGIKK YASSAFVICI TNPLDAMVWA LQKFSGLPTQ KVVGMAGILD SARFRHFLSE EFKISVKDVT AFVLGGHGDS MVPLVRYSTV GGISLPDLVK MGWTTQEKID QIIQRTRDGG AEIVSLLKTG SAFYAPAASA VSMAEAYLKD TKRVVPVAAY LSGQYGVKDT YVGVPVVIGA GGVERVIEID LDKEEKAAFE KSVSAVQKLC EACIAVAPGL K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gaaagaaaat agctcttatt ggttcgggta tgattggggg tactttagca catataattg gacttaaaga acttggtgat gtcgtcttat ttgatattgc agaaggtata ccacaaggta aagctcttga tatcgccgaa tcttcaccag ttgacggttt tgatgtcagt ttaaccggtg ctaattccta tgatgtgatt gaaggtgccg atgttgttat tgtaacagca ggtgttgcaa gaaaacccgg tatgagccgt gatgaccttt tgggcattaa tttaaaggta atggaacaag ttggtgcggg gattaaaaaa tatgcatctt cagcattcgt tatttgtatt acaaatcctc ttgatgcaat ggtctgggct ttgcaaaaat tttcaggtct tccaacacaa aaagtggttg gtatggctgg tattctcgat tcagcacgtt ttcgtcattt cttatcagag gaatttaaga tctctgttaa agatgtgact gcattcgttt taggaggaca cggtgattca atggtgcctt tggtgcgtta ttcaacagtt ggtggtattt ctctacccga tcttgtgaaa atgggctgga cgacgcaaga aaagattgat caaattattc aacgtacccg tgatggtggt gcagaaattg ttagtttgtt aaaaacaggt tctgcttttt atgcaccagc agcttctgct gtctctatgg ctgaagccta tttgaaagac accaagcgtg ttgttcctgt cgcagcttat ctttcagggc aatatggggt caaagatacc tatgttggtg ttccggttgt cattggtgct gggggcgttg agcgcgtgat tgaaatcgat cttgataaag aggagaaagc tgcttttgaa aaatcagtga gtgcagtgca aaaactttgt gaagcttgta ttgctgttgc accaggactc aaataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARKKIALI GSGMIGGTLA HIIGLKELGD VVLFDIAEGI PQGKALDIAE SSPVDGFDVS LTGANSYDVI EGADVVIVTA GVARKPGMSR DDLLGINLKV MEQVGAGIKK YASSAFVICI TNPLDAMVWA LQKFSGLPTQ KVVGMAGILD SARFRHFLSE EFKISVKDVT AFVLGGHGDS MVPLVRYSTV GGISLPDLVK MGWTTQEKID QIIQRTRDGG AEIVSLLKTG SAFYAPAASA VSMAEAYLKD TKRVVPVAAY LSGQYGVKDT YVGVPVVIGA GGVERVIEID LDKEEKAAFE KSVSAVQKLC EACIAVAPGL K
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACGAAAG AAAATAGCTC TTATTGGTTC GGGTATGATT GGGGGTACTT TAGCACATAT AATTGGACTT AAAGAACTTG GTGATGTCGT CTTATTTGAT ATTGCAGAAG GTATACCACA AGGTAAAGCT CTTGATATCG CCGAATCTTC ACCAGTTGAC GGTTTTGATG TCAGTTTAAC CGGTGCTAAT TCCTATGATG TGATTGAAGG TGCCGATGTT GTTATTGTAA CAGCAGGTGT TGCAAGAAAA CCCGGTATGA GCCGTGATGA CCTTTTGGGC ATTAATTTAA AGGTAATGGA ACAAGTTGGT GCGGGGATTA AAAAATATGC ATCTTCAGCA TTCGTTATTT GTATTACAAA TCCTCTTGAT GCAATGGTCT GGGCTTTGCA AAAATTTTCA GGTCTTCCAA CACAAAAAGT GGTTGGTATG GCTGGTATTC TCGATTCAGC ACGTTTTCGT CATTTCTTAT CAGAGGAATT TAAGATCTCT GTTAAAGATG TGACTGCATT CGTTTTAGGA GGACACGGTG ATTCAATGGT GCCTTTGGTG CGTTATTCAA CAGTTGGTGG TATTTCTCTA CCCGATCTTG TGAAAATGGG CTGGACGACG CAAGAAAAGA TTGATCAAAT TATTCAACGT ACCCGTGATG GTGGTGCAGA AATTGTTAGT TTGTTAAAAA CAGGTTCTGC TTTTTATGCA CCAGCAGCTT CTGCTGTCTC TATGGCTGAA GCCTATTTGA AAGACACCAA GCGTGTTGTT CCTGTCGCAG CTTATCTTTC AGGGCAATAT GGGGTCAAAG ATACCTATGT TGGTGTTCCG GTTGTCATTG GTGCTGGGGG CGTTGAGCGC GTGATTGAAA TCGATCTTGA TAAAGAGGAG AAAGCTGCTT TTGAAAAATC AGTGAGTGCA GTGCAAAAAC TTTGTGAAGC TTGTATTGCT GTTGCACCAG GACTCAAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA