BaheA.00657.a
Glycine cleavage system protein t
| CENTER ID: | BaheA.00657.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00657.a.A1.GE25796 | Full length( BaheA.00657.a ) | 1 | 372 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00657.a.A1.PS00127 | Full length( BaheA.00657.a ) | 1 | 372 |
Structures
3GIR
DEPOSITED: 3/6/2009
DETERMINATION: XRay
CLONE: BaheA.00657.a.A1.GE25796
PROTEIN: BaheA.00657.a.A1.PS00127
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127412 |
| PATRIC ID: | fig|283166.5.peg.1365 |
| RefSeq: | YP_034022.1 |
| UniProt: | A0A0H3M6H9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGTSQEFETS SLKTLPLYEL HEKAGAKFGA FAGWRMPLTY PLGVLKEHLH TRAHAGLFDI SHMKLIAVEG PKAVEFLSYA LPVDAALLKI GQSRYSYLLN ERAGILDDLI LTRLAECRFM LVANAGNAQA DFAELEKRAF GFECQVIALE RVLLALQGPQ AAAVLADAGL PGNELLFMQG FEPQQDWFIT RSGYTGEDGF EIALPIGCAR ALAEKLLGDS RVEWVGLAAR DSLRLEAGLC LHGNDITPDT TPIDAALTWA VPKNVREKAQ FYGAKAFLES LQKGPSRCRV GLKPQTRQPI RAGAVLFDNE GNRIGVVTSG GFGPSFDGPV AMGYVPVAWK VEGTEVFTEL RGKKIALSVH SLPFVEQRYF KG
NT Sequence
ATGGGTACAT CACAAGAATT TGAAACATCT TCTTTAAAAA CACTTCCTTT ATATGAATTG CATGAAAAAG CCGGAGCAAA ATTTGGTGCT TTTGCCGGTT GGCGAATGCC TTTGACTTAT CCTCTTGGGG TTTTGAAAGA GCATCTTCAT ACGCGTGCGC ATGCAGGCCT TTTTGATATT TCTCATATGA AATTGATTGC TGTTGAGGGG CCGAAAGCAG TGGAATTTTT ATCTTATGCT CTTCCTGTTG ATGCGGCACT GTTAAAGATT GGTCAATCGC GCTATAGCTA TTTGCTTAAT GAGCGGGCTG GCATTCTTGA TGATCTTATC CTTACTCGTT TGGCAGAGTG CCGCTTTATG CTGGTCGCTA ATGCTGGTAA TGCACAAGCC GACTTTGCTG AACTTGAAAA ACGTGCTTTT GGCTTTGAAT GTCAAGTGAT TGCTCTTGAA AGGGTCTTAC TTGCTCTTCA AGGGCCACAA GCGGCTGCTG TTCTAGCTGA TGCTGGTTTG CCGGGAAATG AACTGTTGTT TATGCAAGGA TTTGAACCAC AACAAGATTG GTTTATCACC CGTTCTGGTT ATACAGGAGA AGATGGTTTT GAAATTGCTC TTCCTATAGG TTGTGCACGC GCATTGGCTG AAAAGCTGCT CGGCGATTCT CGTGTTGAGT GGGTTGGTCT TGCAGCGCGT GACAGTCTTC GATTGGAGGC AGGGTTATGC TTGCATGGCA ATGATATTAC CCCTGATACT ACGCCCATTG ATGCTGCACT GACATGGGCT GTTCCCAAAA ATGTTCGAGA AAAAGCGCAA TTTTATGGAG CAAAGGCTTT TCTGGAATCT CTTCAGAAAG GTCCCTCTCG TTGTCGTGTT GGTTTAAAAC CACAAACACG CCAACCTATT CGTGCTGGTG CTGTGCTTTT CGATAATGAA GGCAATCGGA TTGGGGTGGT AACGTCAGGT GGTTTTGGCC CTTCTTTTGA TGGTCCTGTC GCGATGGGGT ATGTTCCTGT TGCTTGGAAA GTGGAAGGAA CAGAAGTATT TACAGAGCTG CGTGGCAAAA AGATTGCACT GTCTGTTCAT TCACTCCCTT TTGTTGAACA ACGTTATTTT AAAGGA
Details for BaheA.00657.a.A1.GE25796
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGTSQEFET SSLKTLPLYE LHEKAGAKFG AFAGWRMPLT YPLGVLKEHL HTRAHAGLFD ISHMKLIAVE GPKAVEFLSY ALPVDAALLK IGQSRYSYLL NERAGILDDL ILTRLAECRF MLVANAGNAQ ADFAELEKRA FGFECQVIAL ERVLLALQGP QAAAVLADAG LPGNELLFMQ GFEPQQDWFI TRSGYTGXDG FEIALPIGCA RALAEKLLGD SRVEWVGLAA RDSLRLEAGL CLHGNDITPD TTPIDAALTW AVPKNVREKA QFYGAKAXLX SLQKGPXXXX XGLXPQTXQP IXXGXVLXDN EGNRIGVVTX GGFGPSFDGP VAMGYVPVAW KVEGTEVFTX XRGKKXALSV HSLPFVEQRY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggta catcacaaga atttgaaaca tcttctttaa aaacacttcc tttatatgaa ttgcatgaaa aagccggagc aaaatttggt gcttttgccg gttggcgaat gcctttgact tatcctcttg gggttttgaa agagcatctt catacgcgtg cgcatgcagg cctttttgat atttctcata tgaaattgat tgctgttgag gggccgaaag cagtggaatt tttatcttat gctcttcctg ttgatgcggc actgttaaag attggtcaat cgcgctatag ctatttgctt aatgagcggg ctggcattct tgatgatctt atccttactc gtttggcaga gtgccgcttt atgctggtcg ctaatgctgg taatgcacaa gccgactttg ctgaacttga aaaacgtgct tttggctttg aatgtcaagt gattgctctt gaaagggtct tacttgctct tcaagggcca caagcggctg ctgttctagc tgatgctggt ttgccgggaa atgaactgtt gtttatgcaa ggatttgaac cacaacaaga ttggtttatc acccgttctg gttatacagg anaagatggt tttgaaattg ctcttcctat aggttgtgca cgcgcattgg ctgaaaagct gctcggcgat tctcgtgttg agtgggttgg tcttgcagcg cgtgacagtc ttcgattgga ggcagggtta tgcttgcatg gcaatgatat tacccctgat actacgccca ttgatgctgc actgacatgg gctgttccca aaaatgttcg agaaaaagcg caattttatg gagcaaaggc tttnctggna tctcttcaga aaggtcccnn tngtngtngt gnnggtttaa anccacaaac acnccaaccn attngngnng gtnctgtnct ttncgataat gaaggcaatc ggattggggt ggtaacgnca ggtggttttg gcccttcttt tgatggtcct gtcgcgatgg ggtatgttcc tgttgcttgg aaagtggaag gaacagaagt atttacagnn cngcgtggca aaaagantgc actgtctgtt cattcactcc cttttgttga acaacgttat t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGTSQEFET SSLKTLPLYE LHEKAGAKFG AFAGWRMPLT YPLGVLKEHL HTRAHAGLFD ISHMKLIAVE GPKAVEFLSY ALPVDAALLK IGQSRYSYLL NERAGILDDL ILTRLAECRF MLVANAGNAQ ADFAELEKRA FGFECQVIAL ERVLLALQGP QAAAVLADAG LPGNELLFMQ GFEPQQDWFI TRSGYTGEDG FEIALPIGCA RALAEKLLGD SRVEWVGLAA RDSLRLEAGL CLHGNDITPD TTPIDAALTW AVPKNVREKA QFYGAKAFLE SLQKGPSRCR VGLKPQTRQP IRAGAVLFDN EGNRIGVVTS GGFGPSFDGP VAMGYVPVAW KVEGTEVFTE LRGKKIALSV HSLPFVEQRY FKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtacatca caagaatttg aaacatcttc tttaaaaaca cttcctttat atgaattgca tgaaaaagcc ggagcaaaat ttggtgcttt tgccggttgg cgaatgcctt tgacttatcc tcttggggtt ttgaaagagc atcttcatac gcgtgcgcat gcaggccttt ttgatatttc tcatatgaaa ttgattgctg ttgaggggcc gaaagcagtg gaatttttat cttatgctct tcctgttgat gcggcactgt taaagattgg tcaatcgcgc tatagctatt tgcttaatga gcgggctggc attcttgatg atcttatcct tactcgtttg gcagagtgcc gctttatgct ggtcgctaat gctggtaatg cacaagccga ctttgctgaa cttgaaaaac gtgcttttgg ctttgaatgt caagtgattg ctcttgaaag ggtcttactt gctcttcaag ggccacaagc ggctgctgtt ctagctgatg ctggtttgcc gggaaatgaa ctgttgttta tgcaaggatt tgaaccacaa caagattggt ttatcacccg ttctggttat acaggagaag atggttttga aattgctctt cctataggtt gtgcacgcgc attggctgaa aagctgctcg gcgattctcg tgttgagtgg gttggtcttg cagcgcgtga cagtcttcga ttggaggcag ggttatgctt gcatggcaat gatattaccc ctgatactac gcccattgat gctgcactga catgggctgt tcccaaaaat gttcgagaaa aagcgcaatt ttatggagca aaggcttttc tggaatctct tcagaaaggt ccctctcgtt gtcgtgttgg tttaaaacca caaacacgcc aacctattcg tgctggtgct gtgcttttcg ataatgaagg caatcggatt ggggtggtaa cgtcaggtgg ttttggccct tcttttgatg gtcctgtcgc gatggggtat gttcctgttg cttggaaagt ggaaggaaca gaagtattta cagagctgcg tggcaaaaag attgcactgt ctgttcattc actccctttt gttgaacaac gttattttaa aggaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.00657.a.A1.PS00127
| PURIFICATION DATe: | 1/16/2009 |
| CONCENTRATION: | 44.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGTSQEFET SSLKTLPLYE LHEKAGAKFG AFAGWRMPLT YPLGVLKEHL HTRAHAGLFD ISHMKLIAVE GPKAVEFLSY ALPVDAALLK IGQSRYSYLL NERAGILDDL ILTRLAECRF MLVANAGNAQ ADFAELEKRA FGFECQVIAL ERVLLALQGP QAAAVLADAG LPGNELLFMQ GFEPQQDWFI TRSGYTGXDG FEIALPIGCA RALAEKLLGD SRVEWVGLAA RDSLRLEAGL CLHGNDITPD TTPIDAALTW AVPKNVREKA QFYGAKAXLX SLQKGPXXXX XGLXPQTXQP IXXGXVLXDN EGNRIGVVTX GGFGPSFDGP VAMGYVPVAW KVEGTEVFTX XRGKKXALSV HSLPFVEQRY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggta catcacaaga atttgaaaca tcttctttaa aaacacttcc tttatatgaa ttgcatgaaa aagccggagc aaaatttggt gcttttgccg gttggcgaat gcctttgact tatcctcttg gggttttgaa agagcatctt catacgcgtg cgcatgcagg cctttttgat atttctcata tgaaattgat tgctgttgag gggccgaaag cagtggaatt tttatcttat gctcttcctg ttgatgcggc actgttaaag attggtcaat cgcgctatag ctatttgctt aatgagcggg ctggcattct tgatgatctt atccttactc gtttggcaga gtgccgcttt atgctggtcg ctaatgctgg taatgcacaa gccgactttg ctgaacttga aaaacgtgct tttggctttg aatgtcaagt gattgctctt gaaagggtct tacttgctct tcaagggcca caagcggctg ctgttctagc tgatgctggt ttgccgggaa atgaactgtt gtttatgcaa ggatttgaac cacaacaaga ttggtttatc acccgttctg gttatacagg anaagatggt tttgaaattg ctcttcctat aggttgtgca cgcgcattgg ctgaaaagct gctcggcgat tctcgtgttg agtgggttgg tcttgcagcg cgtgacagtc ttcgattgga ggcagggtta tgcttgcatg gcaatgatat tacccctgat actacgccca ttgatgctgc actgacatgg gctgttccca aaaatgttcg agaaaaagcg caattttatg gagcaaaggc tttnctggna tctcttcaga aaggtcccnn tngtngtngt gnnggtttaa anccacaaac acnccaaccn attngngnng gtnctgtnct ttncgataat gaaggcaatc ggattggggt ggtaacgnca ggtggttttg gcccttcttt tgatggtcct gtcgcgatgg ggtatgttcc tgttgcttgg aaagtggaag gaacagaagt atttacagnn cngcgtggca aaaagantgc actgtctgtt cattcactcc cttttgttga acaacgttat t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGTSQEFET SSLKTLPLYE LHEKAGAKFG AFAGWRMPLT YPLGVLKEHL HTRAHAGLFD ISHMKLIAVE GPKAVEFLSY ALPVDAALLK IGQSRYSYLL NERAGILDDL ILTRLAECRF MLVANAGNAQ ADFAELEKRA FGFECQVIAL ERVLLALQGP QAAAVLADAG LPGNELLFMQ GFEPQQDWFI TRSGYTGEDG FEIALPIGCA RALAEKLLGD SRVEWVGLAA RDSLRLEAGL CLHGNDITPD TTPIDAALTW AVPKNVREKA QFYGAKAFLE SLQKGPSRCR VGLKPQTRQP IRAGAVLFDN EGNRIGVVTS GGFGPSFDGP VAMGYVPVAW KVEGTEVFTE LRGKKIALSV HSLPFVEQRY FKG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTACATCA CAAGAATTTG AAACATCTTC TTTAAAAACA CTTCCTTTAT ATGAATTGCA TGAAAAAGCC GGAGCAAAAT TTGGTGCTTT TGCCGGTTGG CGAATGCCTT TGACTTATCC TCTTGGGGTT TTGAAAGAGC ATCTTCATAC GCGTGCGCAT GCAGGCCTTT TTGATATTTC TCATATGAAA TTGATTGCTG TTGAGGGGCC GAAAGCAGTG GAATTTTTAT CTTATGCTCT TCCTGTTGAT GCGGCACTGT TAAAGATTGG TCAATCGCGC TATAGCTATT TGCTTAATGA GCGGGCTGGC ATTCTTGATG ATCTTATCCT TACTCGTTTG GCAGAGTGCC GCTTTATGCT GGTCGCTAAT GCTGGTAATG CACAAGCCGA CTTTGCTGAA CTTGAAAAAC GTGCTTTTGG CTTTGAATGT CAAGTGATTG CTCTTGAAAG GGTCTTACTT GCTCTTCAAG GGCCACAAGC GGCTGCTGTT CTAGCTGATG CTGGTTTGCC GGGAAATGAA CTGTTGTTTA TGCAAGGATT TGAACCACAA CAAGATTGGT TTATCACCCG TTCTGGTTAT ACAGGAGAAG ATGGTTTTGA AATTGCTCTT CCTATAGGTT GTGCACGCGC ATTGGCTGAA AAGCTGCTCG GCGATTCTCG TGTTGAGTGG GTTGGTCTTG CAGCGCGTGA CAGTCTTCGA TTGGAGGCAG GGTTATGCTT GCATGGCAAT GATATTACCC CTGATACTAC GCCCATTGAT GCTGCACTGA CATGGGCTGT TCCCAAAAAT GTTCGAGAAA AAGCGCAATT TTATGGAGCA AAGGCTTTTC TGGAATCTCT TCAGAAAGGT CCCTCTCGTT GTCGTGTTGG TTTAAAACCA CAAACACGCC AACCTATTCG TGCTGGTGCT GTGCTTTTCG ATAATGAAGG CAATCGGATT GGGGTGGTAA CGTCAGGTGG TTTTGGCCCT TCTTTTGATG GTCCTGTCGC GATGGGGTAT GTTCCTGTTG CTTGGAAAGT GGAAGGAACA GAAGTATTTA CAGAGCTGCG TGGCAAAAAG ATTGCACTGT CTGTTCATTC ACTCCCTTTT GTTGAACAAC GTTATTTTAA AGGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA