SSGCID: Target Page

BaheA.00647.a

Two-component response regulator

CENTER ID:	BaheA.00647.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00647.a.A1.GE25794	Full length( BaheA.00647.a )		1	227

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1875
RefSeq:	YP_034307.1
UniProt:	A0A0H3LYQ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNFYTLLIVE NDNDLRPILI EQLQTHKEFK IFQAKTAETG ITIAQEENVD LAIFATELPD LDGHKAVKKL RTQGFRAPII MITNYDTNCD TLLDPETGAN DYVTKPFRFA VLLARIRAQL RQYEQNDDAV LYIGPYTFKP GQKLLIDQQN NKIRLTEKEA AILKYLSCTN EQIVNRETLL EQIWGYNENV VTHTLETHIY RLRQKIEKDP SNAQILITEQ NGYRLNL

NT Sequence

ATGAATTTCT ATACTCTTTT AATTGTTGAA AATGATAATG ATTTGCGCCC TATTCTGATA GAGCAATTGC AAACTCATAA AGAATTTAAA ATTTTCCAAG CAAAAACAGC TGAAACAGGG ATAACAATAG CTCAAGAAGA GAATGTTGAT CTGGCGATTT TTGCGACTGA ACTTCCTGAT CTTGACGGCC ATAAAGCCGT TAAAAAATTA CGCACCCAAG GATTTCGTGC TCCCATTATC ATGATCACGA ATTATGACAC AAATTGCGAT ACTCTTTTAG ATCCTGAAAC AGGTGCCAAT GATTATGTGA CAAAGCCCTT TCGTTTTGCA GTACTTTTGG CACGAATTCG TGCCCAATTG CGCCAATATG AACAAAATGA CGATGCTGTT TTGTACATTG GTCCTTATAC TTTTAAACCT GGACAAAAAC TCCTCATTGA TCAACAAAAC AATAAAATTC GCCTCACAGA AAAGGAGGCT GCCATTCTTA AATATCTCTC TTGCACTAAT GAACAAATCG TCAATCGTGA AACACTTTTA GAACAGATTT GGGGCTATAA TGAAAACGTT GTTACTCATA CCTTAGAGAC CCATATTTAT CGTTTGCGAC AAAAAATTGA AAAAGACCCC TCCAACGCAC AAATCCTCAT TACAGAGCAA AACGGATATC GTCTCAACCT T

Details for BaheA.00647.a.A1.GE25794

HARVESTED ON:	10/31/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNFYTLLIV ENDNDLRPIL IEQLQTHKEF KIFQAKTAET GITIAQEENV DLAIFATELP DLDGHKAVKK LRTQGFRAPI VMITNYDTNC DTLLDPETGA NDYVTKPFRF AVLLARIRAQ LRQYEQNDDA VLYIGPYTFK PGQKLLIDQQ NNKIRLTEKE AAILKYLSCT NEQIVNRETL LEQIWGYNEN VVTHTLETHI YRLRQKIEKD PSNAQILITE QNGYRL

Validated NT Sequence

tgagacgata tccgttttgc tctgtaatga ggatttgtgc gttggagggg tctttttcaa ttttttgtcg caaacgataa atatgggtct ctaaggtatg agtaacaacg ttttcattat agccccaaat ctgttctaaa agtgtttcac gattgacgat ttgttcatta gtgcaagaga gatatttaag aatggcagcc tccttttctg tgaggcgaat tttattgttt tgttgatcaa tgaggagttt ttgtccaggt ttaaaagtat aaggaccaat gtacaaaaca gcatcgtcat tttgttcata ttggcgcaat tgggcacgaa ttcgtgccaa aagtactgca aaacgaaagg gctttgtcac ataatcattg gcacctgttt caggatctaa aagagtatcg caatttgtgt cataattcgt gatcatgaca atgggagcac gaaatccttg ggtgcgtaat tttttaacgg ctttatggcc gtcaagatca ggaagttcag tcgcaaaaat cgccagatca acattctctt cttgagctat tgttatccct gtttcagctg tttttgcttg gaaaatttta aattctttat gagtttgcaa ttgctctatc agaatagggc gcaaatcatt atcattttca acaattaaaa gagtatagaa attcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNFYTLLIV ENDNDLRPIL IEQLQTHKEF KIFQAKTAET GITIAQEENV DLAIFATELP DLDGHKAVKK LRTQGFRAPI IMITNYDTNC DTLLDPETGA NDYVTKPFRF AVLLARIRAQ LRQYEQNDDA VLYIGPYTFK PGQKLLIDQQ NNKIRLTEKE AAILKYLSCT NEQIVNRETL LEQIWGYNEN VVTHTLETHI YRLRQKIEKD PSNAQILITE QNGYRLNL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatttctat actcttttaa ttgttgaaaa tgataatgat ttgcgcccta ttctgataga gcaattgcaa actcataaag aatttaaaat tttccaagca aaaacagctg aaacagggat aacaatagct caagaagaga atgttgatct ggcgattttt gcgactgaac ttcctgatct tgacggccat aaagccgtta aaaaattacg cacccaagga tttcgtgctc ccattatcat gatcacgaat tatgacacaa attgcgatac tcttttagat cctgaaacag gtgccaatga ttatgtgaca aagccctttc gttttgcagt acttttggca cgaattcgtg cccaattgcg ccaatatgaa caaaatgacg atgctgtttt gtacattggt ccttatactt ttaaacctgg acaaaaactc ctcattgatc aacaaaacaa taaaattcgc ctcacagaaa aggaggctgc cattcttaaa tatctctctt gcactaatga acaaatcgtc aatcgtgaaa cacttttaga acagatttgg ggctataatg aaaacgttgt tactcatacc ttagagaccc atatttatcg tttgcgacaa aaaattgaaa aagacccctc caacgcacaa atcctcatta cagagcaaaa cggatatcgt ctcaacctta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a