BaheA.00632.a

succinyl-CoA synthetase subunit beta

CENTER ID: BaheA.00632.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00632.a.A1.GE25792 Full length( BaheA.00632.a ) 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00632.a.A1.PS00125 Full length( BaheA.00632.a ) 1 398

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1926
RefSeq: YP_034346.1
UniProt: Q6G1M1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIHEYQAKR LLHEYGAPIA NGVAVYSVEQ AEKWAKKLPG PLYVVKSQIH AGGRGKGKFK ELDPDAKGGV RLAKSVEEVV ANVKEMLGKT LVTKQTGPEG KQVNRLYIED GADIERELYL SLLVDRNVGR VAFVVSTEGG MDIETVAEET PEKILTLPIN STQGVTSSDC ARLCDALDLH DSAREDGEKL FPILYKAFCE KDMSLLEINP LIVMTNGHLR VLDAKVSFDN NALFRHPDIL ELRDTSEEDP KEIEASKHDL AYVALEGTIG CMVNGAGLAM ATMDIIKLYG AEPANFLDVG GGASKEKVTA AFKIITADPN VKGILVNIFG GIMRCDVIAE GVVAAVREVG LKVPLVVRLE GTNVEQGKAI ISDSGLNVIP ADDLDDAAQK IVAAVKGA
NT Sequence
ATGAATATCC ATGAATATCA GGCCAAACGT CTGCTTCATG AATATGGAGC ACCAATTGCA AATGGTGTTG CTGTTTATTC TGTAGAGCAA GCTGAAAAAT GGGCAAAAAA ATTGCCCGGG CCACTCTATG TGGTTAAAAG TCAGATTCAC GCTGGTGGTC GTGGTAAAGG GAAGTTTAAA GAACTAGATC CTGATGCAAA AGGTGGTGTT CGACTTGCAA AATCCGTTGA AGAAGTCGTT GCAAATGTGA AAGAGATGCT TGGTAAAACT TTGGTCACTA AGCAAACGGG ACCAGAGGGT AAGCAAGTCA ATCGCCTTTA TATTGAAGAT GGTGCTGATA TCGAACGGGA GCTTTATCTT TCGCTTTTGG TTGATCGTAA CGTTGGTCGA GTGGCTTTTG TTGTTTCAAC AGAGGGGGGA ATGGATATTG AAACAGTGGC TGAAGAAACA CCAGAAAAAA TATTAACTCT CCCCATTAAC TCTACCCAAG GTGTTACTTC TTCCGATTGT GCAAGGCTTT GTGATGCATT GGATTTACAC GATAGTGCAC GAGAAGATGG TGAAAAACTT TTCCCAATTC TCTATAAAGC GTTTTGTGAA AAAGATATGA GCCTTCTTGA AATCAATCCG CTTATTGTGA TGACAAATGG TCATTTGCGT GTTCTTGATG CAAAAGTTTC TTTTGATAAT AATGCTCTAT TTCGTCATCC AGACATTTTA GAGTTGCGCG ATACTTCAGA AGAAGATCCA AAAGAGATTG AAGCATCAAA GCATGATCTT GCTTATGTTG CTCTTGAGGG CACAATTGGT TGTATGGTCA ATGGTGCAGG TCTTGCTATG GCAACAATGG ATATCATTAA GCTTTATGGA GCTGAACCAG CAAATTTCCT TGATGTTGGT GGTGGAGCTT CAAAAGAAAA AGTAACCGCT GCTTTTAAGA TTATCACTGC TGATCCAAAT GTTAAGGGCA TTTTGGTTAA TATTTTTGGT GGAATTATGC GTTGTGATGT CATTGCTGAG GGTGTGGTTG CTGCTGTTCG GGAAGTTGGT TTAAAGGTTC CTTTGGTTGT TCGTCTTGAA GGCACGAATG TTGAGCAGGG GAAAGCGATT ATCAGTGATA GTGGTCTGAA CGTTATTCCT GCTGATGATT TAGATGATGC TGCTCAAAAA ATTGTTGCAG CCGTGAAGGG AGCT
Details for BaheA.00632.a.A1.GE25792
HARVESTED ON: 10/31/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMNIHEYQ AKRLLHEYGA PIANGVAVYS VEQAEKWAKK LPGPLYVVKS QIHAGGRGKG KFKELDPDAK GGVRLAKSVE EVVANVKEML GKTLVTKQTG PEGKQVNRLY IEDGADIERE LYLSLLVDRN VGRVAFVVST EGGMDIETVA EETPEKILTL PINSTQGVTS SDCARLCDAL DLHDSAREDG EKLFPILYKA FCEKDMSLLE INPLIVMTNG HLRVLDAKVS FDNNALFRHP DILELRDTSE EDPKEIEASK HDLAYVALEG TIGCMVNGAG LAMATMDIIK LYGAEPANFL DVGGGASKEK VTAAFKIITA DPNVKGILVN IFGGIMRCDV IAEGVVAAVR EVGLKVPLVV RLEGTNVEQG KAIISDSGLN VIPADDLDDA AQKIVAAVKG A
Validated NT Sequence
accntggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga atatccatga atatcaggcc aaacgtctgc ttcatgaata tggagcacca attgcaaatg gtgttgctgt ttattctgta gagcaagctg aaaaatgggc aaaaaaattg cccgggccac tctatgtggt taaaagtcag attcacgctg gtggtcgtgg taaagggaag tttaaagaac tagatcctga tgcaaaaggt ggtgttcgac ttgcaaaatc cgttgaagaa gtcgttgcaa atgtgaaaga gatgcttggt aaaactttgg tcactaagca aacgggacca gagggtaagc aagtcaatcg cctttatatt gaagatggtg ctgatatcga acgggagctt tatctttcgc ttttggttga tcgtaacgtt ggtcgagtgg cttttgttgt ttcaacagag gggggaatgg atattgaaac agtggctgaa gaaacaccag aaaaaatatt aactctcccc attaactcta cccaaggtgt tacttcttcc gattgtgcaa ggctttgtga tgcattggat ttacacgata gtgcacgaga agatggtgaa aaacttttcc caattctcta taaagcgttt tgtgaaaaag atatgagcct tcttgaaatc aatccgctta ttgtgatgac aaatggtcat ttgcgtgttc ttgatgcaaa agtttctttt gataataatg ctctatttcg tcatccagac attttagagt tgcgcgatac ttcagaagaa gatccaaaag agattgaagc atcaaagcat gatcttgctt atgttgctct tgagggcaca attggttgta tggtcaatgg tgcaggtctt gctatggcaa caatggatat cattaagctt tatggagctg aaccagcaaa tttccttgat gttggtggtg gagcttcaaa agaaaaagta accgctgctt ttaagattat cactgctgat ccaaatgtta agggcatttt ggttaatatt tttggtggaa ttatgcgttg tgatgtcatt gctgagggtg tggttgctgc tgttcgggaa gttggtttaa aggttccttt ggttgttcgt cttgaaggca cgaatgttga gcaggggaaa gcgattatca gtgatagtgg tctgaacgtt attcctgctg atgatttaga tgatgctgct caaaaaattg ttgcagccgt gaagggagct taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIHEYQAK RLLHEYGAPI ANGVAVYSVE QAEKWAKKLP GPLYVVKSQI HAGGRGKGKF KELDPDAKGG VRLAKSVEEV VANVKEMLGK TLVTKQTGPE GKQVNRLYIE DGADIERELY LSLLVDRNVG RVAFVVSTEG GMDIETVAEE TPEKILTLPI NSTQGVTSSD CARLCDALDL HDSAREDGEK LFPILYKAFC EKDMSLLEIN PLIVMTNGHL RVLDAKVSFD NNALFRHPDI LELRDTSEED PKEIEASKHD LAYVALEGTI GCMVNGAGLA MATMDIIKLY GAEPANFLDV GGGASKEKVT AAFKIITADP NVKGILVNIF GGIMRCDVIA EGVVAAVREV GLKVPLVVRL EGTNVEQGKA IISDSGLNVI PADDLDDAAQ KIVAAVKGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatatccat gaatatcagg ccaaacgtct gcttcatgaa tatggagcac caattgcaaa tggtgttgct gtttattctg tagagcaagc tgaaaaatgg gcaaaaaaat tgcccgggcc actctatgtg gttaaaagtc agattcacgc tggtggtcgt ggtaaaggga agtttaaaga actagatcct gatgcaaaag gtggtgttcg acttgcaaaa tccgttgaag aagtcgttgc aaatgtgaaa gagatgcttg gtaaaacttt ggtcactaag caaacgggac cagagggtaa gcaagtcaat cgcctttata ttgaagatgg tgctgatatc gaacgggagc tttatctttc gcttttggtt gatcgtaacg ttggtcgagt ggcttttgtt gtttcaacag aggggggaat ggatattgaa acagtggctg aagaaacacc agaaaaaata ttaactctcc ccattaactc tacccaaggt gttacttctt ccgattgtgc aaggctttgt gatgcattgg atttacacga tagtgcacga gaagatggtg aaaaactttt cccaattctc tataaagcgt tttgtgaaaa agatatgagc cttcttgaaa tcaatccgct tattgtgatg acaaatggtc atttgcgtgt tcttgatgca aaagtttctt ttgataataa tgctctattt cgtcatccag acattttaga gttgcgcgat acttcagaag aagatccaaa agagattgaa gcatcaaagc atgatcttgc ttatgttgct cttgagggca caattggttg tatggtcaat ggtgcaggtc ttgctatggc aacaatggat atcattaagc tttatggagc tgaaccagca aatttccttg atgttggtgg tggagcttca aaagaaaaag taaccgctgc ttttaagatt atcactgctg atccaaatgt taagggcatt ttggttaata tttttggtgg aattatgcgt tgtgatgtca ttgctgaggg tgtggttgct gctgttcggg aagttggttt aaaggttcct ttggttgttc gtcttgaagg cacgaatgtt gagcagggga aagcgattat cagtgatagt ggtctgaacg ttattcctgc tgatgattta gatgatgctg ctcaaaaaat tgttgcagcc gtgaagggag ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BaheA.00632.a.A1.PS00125
PURIFICATION DATe: 1/10/2009
CONCENTRATION: 67.53mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMNIHEYQ AKRLLHEYGA PIANGVAVYS VEQAEKWAKK LPGPLYVVKS QIHAGGRGKG KFKELDPDAK GGVRLAKSVE EVVANVKEML GKTLVTKQTG PEGKQVNRLY IEDGADIERE LYLSLLVDRN VGRVAFVVST EGGMDIETVA EETPEKILTL PINSTQGVTS SDCARLCDAL DLHDSAREDG EKLFPILYKA FCEKDMSLLE INPLIVMTNG HLRVLDAKVS FDNNALFRHP DILELRDTSE EDPKEIEASK HDLAYVALEG TIGCMVNGAG LAMATMDIIK LYGAEPANFL DVGGGASKEK VTAAFKIITA DPNVKGILVN IFGGIMRCDV IAEGVVAAVR EVGLKVPLVV RLEGTNVEQG KAIISDSGLN VIPADDLDDA AQKIVAAVKG A
Validated NT Sequence
accntggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga atatccatga atatcaggcc aaacgtctgc ttcatgaata tggagcacca attgcaaatg gtgttgctgt ttattctgta gagcaagctg aaaaatgggc aaaaaaattg cccgggccac tctatgtggt taaaagtcag attcacgctg gtggtcgtgg taaagggaag tttaaagaac tagatcctga tgcaaaaggt ggtgttcgac ttgcaaaatc cgttgaagaa gtcgttgcaa atgtgaaaga gatgcttggt aaaactttgg tcactaagca aacgggacca gagggtaagc aagtcaatcg cctttatatt gaagatggtg ctgatatcga acgggagctt tatctttcgc ttttggttga tcgtaacgtt ggtcgagtgg cttttgttgt ttcaacagag gggggaatgg atattgaaac agtggctgaa gaaacaccag aaaaaatatt aactctcccc attaactcta cccaaggtgt tacttcttcc gattgtgcaa ggctttgtga tgcattggat ttacacgata gtgcacgaga agatggtgaa aaacttttcc caattctcta taaagcgttt tgtgaaaaag atatgagcct tcttgaaatc aatccgctta ttgtgatgac aaatggtcat ttgcgtgttc ttgatgcaaa agtttctttt gataataatg ctctatttcg tcatccagac attttagagt tgcgcgatac ttcagaagaa gatccaaaag agattgaagc atcaaagcat gatcttgctt atgttgctct tgagggcaca attggttgta tggtcaatgg tgcaggtctt gctatggcaa caatggatat cattaagctt tatggagctg aaccagcaaa tttccttgat gttggtggtg gagcttcaaa agaaaaagta accgctgctt ttaagattat cactgctgat ccaaatgtta agggcatttt ggttaatatt tttggtggaa ttatgcgttg tgatgtcatt gctgagggtg tggttgctgc tgttcgggaa gttggtttaa aggttccttt ggttgttcgt cttgaaggca cgaatgttga gcaggggaaa gcgattatca gtgatagtgg tctgaacgtt attcctgctg atgatttaga tgatgctgct caaaaaattg ttgcagccgt gaagggagct taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIHEYQAK RLLHEYGAPI ANGVAVYSVE QAEKWAKKLP GPLYVVKSQI HAGGRGKGKF KELDPDAKGG VRLAKSVEEV VANVKEMLGK TLVTKQTGPE GKQVNRLYIE DGADIERELY LSLLVDRNVG RVAFVVSTEG GMDIETVAEE TPEKILTLPI NSTQGVTSSD CARLCDALDL HDSAREDGEK LFPILYKAFC EKDMSLLEIN PLIVMTNGHL RVLDAKVSFD NNALFRHPDI LELRDTSEED PKEIEASKHD LAYVALEGTI GCMVNGAGLA MATMDIIKLY GAEPANFLDV GGGASKEKVT AAFKIITADP NVKGILVNIF GGIMRCDVIA EGVVAAVREV GLKVPLVVRL EGTNVEQGKA IISDSGLNVI PADDLDDAAQ KIVAAVKGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATATCCAT GAATATCAGG CCAAACGTCT GCTTCATGAA TATGGAGCAC CAATTGCAAA TGGTGTTGCT GTTTATTCTG TAGAGCAAGC TGAAAAATGG GCAAAAAAAT TGCCCGGGCC ACTCTATGTG GTTAAAAGTC AGATTCACGC TGGTGGTCGT GGTAAAGGGA AGTTTAAAGA ACTAGATCCT GATGCAAAAG GTGGTGTTCG ACTTGCAAAA TCCGTTGAAG AAGTCGTTGC AAATGTGAAA GAGATGCTTG GTAAAACTTT GGTCACTAAG CAAACGGGAC CAGAGGGTAA GCAAGTCAAT CGCCTTTATA TTGAAGATGG TGCTGATATC GAACGGGAGC TTTATCTTTC GCTTTTGGTT GATCGTAACG TTGGTCGAGT GGCTTTTGTT GTTTCAACAG AGGGGGGAAT GGATATTGAA ACAGTGGCTG AAGAAACACC AGAAAAAATA TTAACTCTCC CCATTAACTC TACCCAAGGT GTTACTTCTT CCGATTGTGC AAGGCTTTGT GATGCATTGG ATTTACACGA TAGTGCACGA GAAGATGGTG AAAAACTTTT CCCAATTCTC TATAAAGCGT TTTGTGAAAA AGATATGAGC CTTCTTGAAA TCAATCCGCT TATTGTGATG ACAAATGGTC ATTTGCGTGT TCTTGATGCA AAAGTTTCTT TTGATAATAA TGCTCTATTT CGTCATCCAG ACATTTTAGA GTTGCGCGAT ACTTCAGAAG AAGATCCAAA AGAGATTGAA GCATCAAAGC ATGATCTTGC TTATGTTGCT CTTGAGGGCA CAATTGGTTG TATGGTCAAT GGTGCAGGTC TTGCTATGGC AACAATGGAT ATCATTAAGC TTTATGGAGC TGAACCAGCA AATTTCCTTG ATGTTGGTGG TGGAGCTTCA AAAGAAAAAG TAACCGCTGC TTTTAAGATT ATCACTGCTG ATCCAAATGT TAAGGGCATT TTGGTTAATA TTTTTGGTGG AATTATGCGT TGTGATGTCA TTGCTGAGGG TGTGGTTGCT GCTGTTCGGG AAGTTGGTTT AAAGGTTCCT TTGGTTGTTC GTCTTGAAGG CACGAATGTT GAGCAGGGGA AAGCGATTAT CAGTGATAGT GGTCTGAACG TTATTCCTGC TGATGATTTA GATGATGCTG CTCAAAAAAT TGTTGCAGCC GTGAAGGGAG CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA