BaheA.00617.a
glyceraldehyde-3-phosphate dehydrogenase
| CENTER ID: | BaheA.00617.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00617.a.A1.GE25791 | Full length( BaheA.00617.a ) | 1 | 335 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00617.a.A1.PS00362 | Full length( BaheA.00617.a ) | 1 | 335 |
Structures
3L0D
DEPOSITED: 12/9/2009
DETERMINATION: XRay
CLONE: BaheA.00617.a.A1.GE25791
PROTEIN: BaheA.00617.a.A1.PS00362
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126628 |
| PATRIC ID: | fig|283166.5.peg.1736 |
| RefSeq: | YP_034206.1 |
| UniProt: | Q8L201 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVRVAINGF GRIGRNILRA IIESGRQDIE VVALNDLGSV ETNAHLLRYD SVHGCFPGTV QVVGDAIDIG SSLIKVFAER DPAQLPWKAL DIDIALECTG IFTARDKASA HLDAGAKRVL VSAPSEGADL TVVYGVNHQF LSKEHHVISN ASCTTNCLAP VAQVLHNTVG IEKGFMTTIH SYTGDQPVLD TMHRDLYRAR AAALSMIPTS TGAAKAVGLV LPELKGLLDG VSIRVPTPNV SVVDLTFTAK RSTTIEEINT AIRTAAQGSL KGILDYTDEK LVSCDFNHNP HSAIFHNDQT KVIDGQLCRV LVWYDNEWGF SNRMCDTAVA FAKTI
NT Sequence
ATGAGTGTTC GCGTTGCAAT TAATGGGTTT GGGCGTATCG GACGCAATAT TTTGCGTGCC ATTATCGAAA GTGGACGACA AGATATTGAA GTTGTGGCCC TTAATGATTT AGGATCGGTG GAAACCAATG CCCATTTGTT GCGCTATGAT TCTGTTCATG GATGTTTCCC TGGCACTGTT CAAGTTGTTG GTGATGCCAT TGATATCGGT TCTAGTTTGA TAAAAGTGTT TGCAGAGCGT GATCCAGCAC AATTGCCTTG GAAGGCTTTG GATATTGATA TTGCTTTGGA GTGTACAGGT ATTTTTACCG CTCGTGACAA AGCAAGTGCC CACTTGGATG CGGGGGCAAA GCGTGTTCTT GTTTCTGCAC CCTCAGAAGG CGCAGATCTT ACTGTGGTTT ATGGAGTAAA TCATCAGTTT TTGAGCAAAG AACACCATGT TATTTCGAAT GCTTCTTGCA CAACCAACTG TTTGGCGCCT GTTGCACAAG TTTTGCACAA CACTGTTGGC ATTGAAAAGG GCTTTATGAC AACAATCCAT TCTTATACGG GCGATCAACC TGTGTTGGAT ACAATGCATC GTGACCTTTA TCGTGCACGT GCGGCGGCTC TTTCTATGAT CCCTACCTCA ACAGGGGCAG CGAAAGCTGT GGGATTGGTT TTGCCAGAAT TAAAAGGATT GCTTGATGGG GTGTCGATTC GCGTTCCAAC GCCGAATGTT TCCGTTGTTG ATTTGACGTT TACTGCTAAG CGTTCCACAA CGATTGAAGA AATTAATACT GCTATTCGTA CTGCTGCACA GGGGTCACTC AAGGGAATTT TAGACTATAC TGACGAAAAG CTCGTGAGTT GTGATTTTAA CCATAATCCA CATTCTGCTA TTTTTCATAA TGATCAAACC AAGGTCATTG ATGGTCAACT TTGTCGTGTT CTGGTTTGGT ATGATAATGA ATGGGGTTTC TCAAACCGCA TGTGTGATAC GGCTGTGGCT TTTGCTAAAA CAATA
Details for BaheA.00617.a.A1.GE25791
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMSVRVAI NGFGRIGRNI LRAIIESGRQ DIEVVALNDL GSVETNAHLL RYDSVHGCFP GTVQVVGDAI DIGSSLIKVF AERDPAQLPW KALDIDIALE CTGIFTARDK ASAHLDAGAK RVLVSAPSEG ADLTVVYGVN HQFLSKEHHV ISNASCTTNC LAPVAQVLHN TVGIEKGFMT TIHSYTGDQP VLDTMHRDLY RARAAALSMI PTSTGAAKAV GLVLPELKGL LDGVSIRVPT PNVSVVDLTF TAKRSTTIEE INTAIRTAAQ GSLKGILDYT DEKLVSCDFN HNPHSAIFHN DQTKVIDGQL CRVLVWYDNE WGFSNRMCDT AVAFAKTI
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgttcgc gttgcaatta atgggtttgg gcgtatcgga cgcaatattt tgcgtgccat tatcgaaagt ggacgacaag atattgaagt tgtggccctt aatgatttag gatcggtgga aaccaatgcc catttgttgc gctatgattc tgttcatgga tgtttccctg gcactgttca agttgttggt gatgccattg atatcggttc tagtttgata aaagtgtttg cagagcgtga tccagcacaa ttgccttgga aggctttgga tattgatatt gctttggagt gtacaggtat ttttaccgct cgtgacaaag caagtgccca cttggatgcg ggggcaaagc gtgttcttgt ttctgcaccc tcagaaggcg cagatcttac tgtggtttat ggagtaaatc atcagttttt gagcaaagaa caccatgtta tttcgaatgc ttcttgcaca accaactgtt tggcgcctgt tgcacaagtt ttgcacaaca ctgttggcat tgaaaagggc tttatgacaa caatccattc ttatacgggc gatcaacctg tgttggatac aatgcatcgt gacctttatc gtgcacgtgc ggcggctctt tctatgatcc ctacctcaac aggggcagcg aaagctgtgg gattggtttt gccagaatta aaaggattgc ttgatggggt gtcgattcgc gttccaacgc cgaatgtttc cgttgttgat ttgacgttta ctgctaagcg ttccacaacg attgaagaaa ttaatactgc tattcgtact gctgcacagg ggtcactcaa gggaatttta gactatactg acgaaaagct cgtgagttgt gattttaacc ataatccaca ttctgctatt tttcataatg atcaaaccaa ggtcattgat ggtcaacttt gtcgtgttct ggtttggtat gataatgaat ggggtttctc aaaccgcatg tgtgatacgg ctgtggcttt tgctaaaaca atataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVRVAING FGRIGRNILR AIIESGRQDI EVVALNDLGS VETNAHLLRY DSVHGCFPGT VQVVGDAIDI GSSLIKVFAE RDPAQLPWKA LDIDIALECT GIFTARDKAS AHLDAGAKRV LVSAPSEGAD LTVVYGVNHQ FLSKEHHVIS NASCTTNCLA PVAQVLHNTV GIEKGFMTTI HSYTGDQPVL DTMHRDLYRA RAAALSMIPT STGAAKAVGL VLPELKGLLD GVSIRVPTPN VSVVDLTFTA KRSTTIEEIN TAIRTAAQGS LKGILDYTDE KLVSCDFNHN PHSAIFHNDQ TKVIDGQLCR VLVWYDNEWG FSNRMCDTAV AFAKTI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgttcgc gttgcaatta atgggtttgg gcgtatcgga cgcaatattt tgcgtgccat tatcgaaagt ggacgacaag atattgaagt tgtggccctt aatgatttag gatcggtgga aaccaatgcc catttgttgc gctatgattc tgttcatgga tgtttccctg gcactgttca agttgttggt gatgccattg atatcggttc tagtttgata aaagtgtttg cagagcgtga tccagcacaa ttgccttgga aggctttgga tattgatatt gctttggagt gtacaggtat ttttaccgct cgtgacaaag caagtgccca cttggatgcg ggggcaaagc gtgttcttgt ttctgcaccc tcagaaggcg cagatcttac tgtggtttat ggagtaaatc atcagttttt gagcaaagaa caccatgtta tttcgaatgc ttcttgcaca accaactgtt tggcgcctgt tgcacaagtt ttgcacaaca ctgttggcat tgaaaagggc tttatgacaa caatccattc ttatacgggc gatcaacctg tgttggatac aatgcatcgt gacctttatc gtgcacgtgc ggcggctctt tctatgatcc ctacctcaac aggggcagcg aaagctgtgg gattggtttt gccagaatta aaaggattgc ttgatggggt gtcgattcgc gttccaacgc cgaatgtttc cgttgttgat ttgacgttta ctgctaagcg ttccacaacg attgaagaaa ttaatactgc tattcgtact gctgcacagg ggtcactcaa gggaatttta gactatactg acgaaaagct cgtgagttgt gattttaacc ataatccaca ttctgctatt tttcataatg atcaaaccaa ggtcattgat ggtcaacttt gtcgtgttct ggtttggtat gataatgaat ggggtttctc aaaccgcatg tgtgatacgg ctgtggcttt tgctaaaaca ataaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BaheA.00617.a.A1.PS00362
| PURIFICATION DATe: | 9/22/2009 |
| CONCENTRATION: | 23.65mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSVRVAI NGFGRIGRNI LRAIIESGRQ DIEVVALNDL GSVETNAHLL RYDSVHGCFP GTVQVVGDAI DIGSSLIKVF AERDPAQLPW KALDIDIALE CTGIFTARDK ASAHLDAGAK RVLVSAPSEG ADLTVVYGVN HQFLSKEHHV ISNASCTTNC LAPVAQVLHN TVGIEKGFMT TIHSYTGDQP VLDTMHRDLY RARAAALSMI PTSTGAAKAV GLVLPELKGL LDGVSIRVPT PNVSVVDLTF TAKRSTTIEE INTAIRTAAQ GSLKGILDYT DEKLVSCDFN HNPHSAIFHN DQTKVIDGQL CRVLVWYDNE WGFSNRMCDT AVAFAKTI
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgttcgc gttgcaatta atgggtttgg gcgtatcgga cgcaatattt tgcgtgccat tatcgaaagt ggacgacaag atattgaagt tgtggccctt aatgatttag gatcggtgga aaccaatgcc catttgttgc gctatgattc tgttcatgga tgtttccctg gcactgttca agttgttggt gatgccattg atatcggttc tagtttgata aaagtgtttg cagagcgtga tccagcacaa ttgccttgga aggctttgga tattgatatt gctttggagt gtacaggtat ttttaccgct cgtgacaaag caagtgccca cttggatgcg ggggcaaagc gtgttcttgt ttctgcaccc tcagaaggcg cagatcttac tgtggtttat ggagtaaatc atcagttttt gagcaaagaa caccatgtta tttcgaatgc ttcttgcaca accaactgtt tggcgcctgt tgcacaagtt ttgcacaaca ctgttggcat tgaaaagggc tttatgacaa caatccattc ttatacgggc gatcaacctg tgttggatac aatgcatcgt gacctttatc gtgcacgtgc ggcggctctt tctatgatcc ctacctcaac aggggcagcg aaagctgtgg gattggtttt gccagaatta aaaggattgc ttgatggggt gtcgattcgc gttccaacgc cgaatgtttc cgttgttgat ttgacgttta ctgctaagcg ttccacaacg attgaagaaa ttaatactgc tattcgtact gctgcacagg ggtcactcaa gggaatttta gactatactg acgaaaagct cgtgagttgt gattttaacc ataatccaca ttctgctatt tttcataatg atcaaaccaa ggtcattgat ggtcaacttt gtcgtgttct ggtttggtat gataatgaat ggggtttctc aaaccgcatg tgtgatacgg ctgtggcttt tgctaaaaca atataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVRVAING FGRIGRNILR AIIESGRQDI EVVALNDLGS VETNAHLLRY DSVHGCFPGT VQVVGDAIDI GSSLIKVFAE RDPAQLPWKA LDIDIALECT GIFTARDKAS AHLDAGAKRV LVSAPSEGAD LTVVYGVNHQ FLSKEHHVIS NASCTTNCLA PVAQVLHNTV GIEKGFMTTI HSYTGDQPVL DTMHRDLYRA RAAALSMIPT STGAAKAVGL VLPELKGLLD GVSIRVPTPN VSVVDLTFTA KRSTTIEEIN TAIRTAAQGS LKGILDYTDE KLVSCDFNHN PHSAIFHNDQ TKVIDGQLCR VLVWYDNEWG FSNRMCDTAV AFAKTI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGTTCGC GTTGCAATTA ATGGGTTTGG GCGTATCGGA CGCAATATTT TGCGTGCCAT TATCGAAAGT GGACGACAAG ATATTGAAGT TGTGGCCCTT AATGATTTAG GATCGGTGGA AACCAATGCC CATTTGTTGC GCTATGATTC TGTTCATGGA TGTTTCCCTG GCACTGTTCA AGTTGTTGGT GATGCCATTG ATATCGGTTC TAGTTTGATA AAAGTGTTTG CAGAGCGTGA TCCAGCACAA TTGCCTTGGA AGGCTTTGGA TATTGATATT GCTTTGGAGT GTACAGGTAT TTTTACCGCT CGTGACAAAG CAAGTGCCCA CTTGGATGCG GGGGCAAAGC GTGTTCTTGT TTCTGCACCC TCAGAAGGCG CAGATCTTAC TGTGGTTTAT GGAGTAAATC ATCAGTTTTT GAGCAAAGAA CACCATGTTA TTTCGAATGC TTCTTGCACA ACCAACTGTT TGGCGCCTGT TGCACAAGTT TTGCACAACA CTGTTGGCAT TGAAAAGGGC TTTATGACAA CAATCCATTC TTATACGGGC GATCAACCTG TGTTGGATAC AATGCATCGT GACCTTTATC GTGCACGTGC GGCGGCTCTT TCTATGATCC CTACCTCAAC AGGGGCAGCG AAAGCTGTGG GATTGGTTTT GCCAGAATTA AAAGGATTGC TTGATGGGGT GTCGATTCGC GTTCCAACGC CGAATGTTTC CGTTGTTGAT TTGACGTTTA CTGCTAAGCG TTCCACAACG ATTGAAGAAA TTAATACTGC TATTCGTACT GCTGCACAGG GGTCACTCAA GGGAATTTTA GACTATACTG ACGAAAAGCT CGTGAGTTGT GATTTTAACC ATAATCCACA TTCTGCTATT TTTCATAATG ATCAAACCAA GGTCATTGAT GGTCAACTTT GTCGTGTTCT GGTTTGGTAT GATAATGAAT GGGGTTTCTC AAACCGCATG TGTGATACGG CTGTGGCTTT TGCTAAAACA ATAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA