BaheA.00606.a
2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase
| CENTER ID: | BaheA.00606.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00606.a.A1.GE25789 | Full length( BaheA.00606.a ) | 1 | 282 | ||
| BaheA.00606.a.AE1.GE43123 | Full length( BaheA.00606.a ) | 1 | 282 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00606.a.A1.PS00132 | Full length( BaheA.00606.a ) | 1 | 282 | ||
| BaheA.00606.a.AE1.PS38599 | Full length( BaheA.00606.a ) | 1 | 282 | ||
| BaheA.00606.a.AE1.PS38600 | Full length( BaheA.00606.a ) | 1 | 282 | ||
| BaheA.00606.a.A1.PW26985 | Full length( BaheA.00606.a ) | 1 | 282 |
Structures
6WQM
DEPOSITED: 4/29/2020
DETERMINATION: XRay
CLONE: BaheA.00606.a.AE1.GE43123
PROTEIN: BaheA.00606.a.AE1.PS38599
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_134244 |
| PATRIC ID: | fig|283166.5.peg.86 |
| RefSeq: | YP_032935.1 |
| UniProt: | Q6G549 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RD
NT Sequence
ATGACTGATC TCACACAACT TGAAATGATT ATTGAAAAAG CATTTGACGA CCGCAACTCT ATCAATACAA CCACTAAGGG TGAAATTCTT GAGAGTGTCG AACATGCGTT AAACCTTCTT GATAAAGGTG AAGTCCGTGT TGTAAAACGT CAAAAAAATG GAAAATGGCA CGTTCATCAA TGGTTGAAAA AAGCCGTTCT TCTTTCTTTT CGGCTCAATC CTATGCAAAT TATGACCGGT GGAGTAAATG GAACATCTTG GTGGGATAAA GTCCCTTCAA AATTTTCTCA TTGGCAAGAA GCTGACTTTA AAAAAGCGGA TTTCCGTTCC GTTCCTGGAG CGATTGTACG CCATTCTGCT TATATTGCAC CCAATGTTAT TCTGATGCCA TCCTTTGTCA ACCTTGGTGC TTTTGTTGAT GAAGGAACAA TGGTCGATAC ATGGGCCACT GTTGGTTCTT GTGCACAAAT TGGCAAACAT GTTCATCTTT CTGGTGGTGT TGGTATTGGA GGTGTTTTAG AACCACTGCA AGCAAACCCA ACGATTATTG AAGATCATTG TTTTATTGGT GCACGATCTG AAGTCGTTGA AGGCTGTATT ATTCGTGAAG GCTCTGTTTT AGGAATGGGA GTCTTTATTG GAAAATCTAC AAAAATTATT GACCGTACAA CAGGTGAAAT TTTTATAGGC GAAGTCCCTC CCTATTCTGT CGTTGTTCCA GGTTCTCTTC CCGGAAAACC ACTCCCAAAC GGTGAAATAG GCCCTAATCT TTATTGTGCT GTTATCGTGA AACGCGTTGA CCAAAAAACA CGAGAAAAAA CATCTATTAA TGATCTTTTG CGTGAC
Details for BaheA.00606.a.A1.GE25789
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLTQLEM IIEKAFDDRN SINTTTKGEI LESVEHALNL LDKGEVRVVK XQKNGKWHVH QWLKKAVLLS FRLNPMQIMT GGVNGTSWWD KVPSKFSHWQ EADFKKADFR SVPGAIVRHS AYIAPNVILM PSFVNLGAFV DEGTMVDTWA TVGSCAQIGK HVHLSGGVGI GGVLEPLQAN PTIIEDHCFI GARSEVVEGC IIREGSVLGM GVFIGKSTKI IDRTTGEIFI GEVPPYSVVV PGSLPGKPLP NGEIGPNLYC AVIVKRVDQK TREKTSINDL LRD
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgactgat ctcacacaac ttgaaatgat tattgaaaaa gcatttgacg accgcaactc tatcaataca accactaagg gtgaaattct tgagagtgtc gaacatgcgt taaaccttct tgataaaggt gaagtccgtg ttgtaaaacn tcaaaaaaat ggaaaatggc acgttcatca atggttgaaa aaagccgttc ttctttcttt tcggctcaat cctatgcaaa ttatgaccgg tggagtaaat ggaacatctt ggtgggataa agtcccttca aaattttctc attggcaaga agctgacttt aaaaaagcgg atttccgttc cgttcctgga gcgattgtac gccattctgc ttatattgca cccaatgtta ttctgatgcc atcctttgtc aaccttggtg cttttgttga tgaaggaaca atggtcgata catgggccac tgttggttct tgtgcacaaa ttggcaaaca tgttcatctt tctggtggtg ttggtattgg aggtgtttta gaaccactgc aagcaaaccc aacgattatt gaagatcatt gttttattgg tgcacgatct gaagtcgttg aaggctgtat tattcgtgaa ggctctgttt taggaatggg agtctttatt ggaaaatcta caaaaattat tgaccgtaca acaggtgaaa tttttatagg cgaagtccct ccctattctg tcgttgttcc aggttctctt cccggaaaac cactcccaaa cggtgaaata ggccctaatc tttattgtgc tgttatcgtg aaacgcgttg accaaaaaac acgagaaaaa acatctatta atgatctttt gcgtgactaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLTQLEM IIEKAFDDRN SINTTTKGEI LESVEHALNL LDKGEVRVVK RQKNGKWHVH QWLKKAVLLS FRLNPMQIMT GGVNGTSWWD KVPSKFSHWQ EADFKKADFR SVPGAIVRHS AYIAPNVILM PSFVNLGAFV DEGTMVDTWA TVGSCAQIGK HVHLSGGVGI GGVLEPLQAN PTIIEDHCFI GARSEVVEGC IIREGSVLGM GVFIGKSTKI IDRTTGEIFI GEVPPYSVVV PGSLPGKPLP NGEIGPNLYC AVIVKRVDQK TREKTSINDL LRD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatctc acacaacttg aaatgattat tgaaaaagca tttgacgacc gcaactctat caatacaacc actaagggtg aaattcttga gagtgtcgaa catgcgttaa accttcttga taaaggtgaa gtccgtgttg taaaacgtca aaaaaatgga aaatggcacg ttcatcaatg gttgaaaaaa gccgttcttc tttcttttcg gctcaatcct atgcaaatta tgaccggtgg agtaaatgga acatcttggt gggataaagt cccttcaaaa ttttctcatt ggcaagaagc tgactttaaa aaagcggatt tccgttccgt tcctggagcg attgtacgcc attctgctta tattgcaccc aatgttattc tgatgccatc ctttgtcaac cttggtgctt ttgttgatga aggaacaatg gtcgatacat gggccactgt tggttcttgt gcacaaattg gcaaacatgt tcatctttct ggtggtgttg gtattggagg tgttttagaa ccactgcaag caaacccaac gattattgaa gatcattgtt ttattggtgc acgatctgaa gtcgttgaag gctgtattat tcgtgaaggc tctgttttag gaatgggagt ctttattgga aaatctacaa aaattattga ccgtacaaca ggtgaaattt ttataggcga agtccctccc tattctgtcg ttgttccagg ttctcttccc ggaaaaccac tcccaaacgg tgaaataggc cctaatcttt attgtgctgt tatcgtgaaa cgcgttgacc aaaaaacacg agaaaaaaca tctattaatg atcttttgcg tgacaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.00606.a.AE1.GE43123
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RDAGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgtcacgcaa aagatcatta atagatgttt tttctcgtgt tttttggtca acgcgtttca cgataacagc acaataaaga ttagggccta tttcaccgtt tgggagtggt tttccgggaa gagaacctgg aacaacgaca gaatagggag ggacttcgcc tataaaaatt tcacctgttg tacggtcaat aatttttgta gattttccaa taaagactcc cattcctaaa acagagcctt cacgaataat acagccttca acgacttcag atcgtgcacc aataaaacaa tgatcttcaa taatcgttgg gtttgcttgc agtggttcta aaacacctcc aataccaaca ccaccagaaa gatgaacatg tttgccaatt tgtgcacaag aaccaacagt ggcccatgta tcgaccattg ttccttcatc aacaaaagca ccaaggttga caaaggatgg catcagaata acattgggtg caatataagc agaatggcgt acaatcgctc caggaacgga acggaaatcc gcttttttaa agtcagcttc ttgccaatga gaaaattttg aagggacttt atcccaccaa gatgttccat ttactccacc ggtcataatt tgcataggat tgagccgaaa agaaagaaga acggcttttt tcaaccattg atgaacgtgc cattttccat ttttttgacg ttttacaaca cggacttcac ctttatcaag aaggtttaac gcatgttcga cactctcaag aatttcaccc ttagtggttg tattgataga gttgcggtcg tcaaatgctt tttcaataat catttcaagt tgtgtgagat cagtcatggg agaga
Expected Protein Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RDGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gactgatctc acacaacttg aaatgattat tgaaaaagca tttgacgacc gcaactctat caatacaacc actaagggtg aaattcttga gagtgtcgaa catgcgttaa accttcttga taaaggtgaa gtccgtgttg taaaacgtca aaaaaatgga aaatggcacg ttcatcaatg gttgaaaaaa gccgttcttc tttcttttcg gctcaatcct atgcaaatta tgaccggtgg agtaaatgga acatcttggt gggataaagt cccttcaaaa ttttctcatt ggcaagaagc tgactttaaa aaagcggatt tccgttccgt tcctggagcg attgtacgcc attctgctta tattgcaccc aatgttattc tgatgccatc ctttgtcaac cttggtgctt ttgttgatga aggaacaatg gtcgatacat gggccactgt tggttcttgt gcacaaattg gcaaacatgt tcatctttct ggtggtgttg gtattggagg tgttttagaa ccactgcaag caaacccaac gattattgaa gatcattgtt ttattggtgc acgatctgaa gtcgttgaag gctgtattat tcgtgaaggc tctgttttag gaatgggagt ctttattgga aaatctacaa aaattattga ccgtacaaca ggtgaaattt ttataggcga agtccctccc tattctgtcg ttgttccagg ttctcttccc ggaaaaccac tcccaaacgg tgaaataggc cctaatcttt attgtgctgt tatcgtgaaa cgcgttgacc aaaaaacacg agaaaaaaca tctattaatg atcttttgcg tgacgggcac caccatcatc atcattaacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
Details for BaheA.00606.a.A1.PS00132
| PURIFICATION DATe: | 1/9/2009 |
| CONCENTRATION: | 26.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 21 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLTQLEM IIEKAFDDRN SINTTTKGEI LESVEHALNL LDKGEVRVVK XQKNGKWHVH QWLKKAVLLS FRLNPMQIMT GGVNGTSWWD KVPSKFSHWQ EADFKKADFR SVPGAIVRHS AYIAPNVILM PSFVNLGAFV DEGTMVDTWA TVGSCAQIGK HVHLSGGVGI GGVLEPLQAN PTIIEDHCFI GARSEVVEGC IIREGSVLGM GVFIGKSTKI IDRTTGEIFI GEVPPYSVVV PGSLPGKPLP NGEIGPNLYC AVIVKRVDQK TREKTSINDL LRD
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgactgat ctcacacaac ttgaaatgat tattgaaaaa gcatttgacg accgcaactc tatcaataca accactaagg gtgaaattct tgagagtgtc gaacatgcgt taaaccttct tgataaaggt gaagtccgtg ttgtaaaacn tcaaaaaaat ggaaaatggc acgttcatca atggttgaaa aaagccgttc ttctttcttt tcggctcaat cctatgcaaa ttatgaccgg tggagtaaat ggaacatctt ggtgggataa agtcccttca aaattttctc attggcaaga agctgacttt aaaaaagcgg atttccgttc cgttcctgga gcgattgtac gccattctgc ttatattgca cccaatgtta ttctgatgcc atcctttgtc aaccttggtg cttttgttga tgaaggaaca atggtcgata catgggccac tgttggttct tgtgcacaaa ttggcaaaca tgttcatctt tctggtggtg ttggtattgg aggtgtttta gaaccactgc aagcaaaccc aacgattatt gaagatcatt gttttattgg tgcacgatct gaagtcgttg aaggctgtat tattcgtgaa ggctctgttt taggaatggg agtctttatt ggaaaatcta caaaaattat tgaccgtaca acaggtgaaa tttttatagg cgaagtccct ccctattctg tcgttgttcc aggttctctt cccggaaaac cactcccaaa cggtgaaata ggccctaatc tttattgtgc tgttatcgtg aaacgcgttg accaaaaaac acgagaaaaa acatctatta atgatctttt gcgtgactaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLTQLEM IIEKAFDDRN SINTTTKGEI LESVEHALNL LDKGEVRVVK RQKNGKWHVH QWLKKAVLLS FRLNPMQIMT GGVNGTSWWD KVPSKFSHWQ EADFKKADFR SVPGAIVRHS AYIAPNVILM PSFVNLGAFV DEGTMVDTWA TVGSCAQIGK HVHLSGGVGI GGVLEPLQAN PTIIEDHCFI GARSEVVEGC IIREGSVLGM GVFIGKSTKI IDRTTGEIFI GEVPPYSVVV PGSLPGKPLP NGEIGPNLYC AVIVKRVDQK TREKTSINDL LRD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGATCTC ACACAACTTG AAATGATTAT TGAAAAAGCA TTTGACGACC GCAACTCTAT CAATACAACC ACTAAGGGTG AAATTCTTGA GAGTGTCGAA CATGCGTTAA ACCTTCTTGA TAAAGGTGAA GTCCGTGTTG TAAAACGTCA AAAAAATGGA AAATGGCACG TTCATCAATG GTTGAAAAAA GCCGTTCTTC TTTCTTTTCG GCTCAATCCT ATGCAAATTA TGACCGGTGG AGTAAATGGA ACATCTTGGT GGGATAAAGT CCCTTCAAAA TTTTCTCATT GGCAAGAAGC TGACTTTAAA AAAGCGGATT TCCGTTCCGT TCCTGGAGCG ATTGTACGCC ATTCTGCTTA TATTGCACCC AATGTTATTC TGATGCCATC CTTTGTCAAC CTTGGTGCTT TTGTTGATGA AGGAACAATG GTCGATACAT GGGCCACTGT TGGTTCTTGT GCACAAATTG GCAAACATGT TCATCTTTCT GGTGGTGTTG GTATTGGAGG TGTTTTAGAA CCACTGCAAG CAAACCCAAC GATTATTGAA GATCATTGTT TTATTGGTGC ACGATCTGAA GTCGTTGAAG GCTGTATTAT TCGTGAAGGC TCTGTTTTAG GAATGGGAGT CTTTATTGGA AAATCTACAA AAATTATTGA CCGTACAACA GGTGAAATTT TTATAGGCGA AGTCCCTCCC TATTCTGTCG TTGTTCCAGG TTCTCTTCCC GGAAAACCAC TCCCAAACGG TGAAATAGGC CCTAATCTTT ATTGTGCTGT TATCGTGAAA CGCGTTGACC AAAAAACACG AGAAAAAACA TCTATTAATG ATCTTTTGCG TGACAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BaheA.00606.a.AE1.PS38599
| PURIFICATION DATe: | 9/12/2019 |
| CONCENTRATION: | 31.38mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RDAGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgtcacgcaa aagatcatta atagatgttt tttctcgtgt tttttggtca acgcgtttca cgataacagc acaataaaga ttagggccta tttcaccgtt tgggagtggt tttccgggaa gagaacctgg aacaacgaca gaatagggag ggacttcgcc tataaaaatt tcacctgttg tacggtcaat aatttttgta gattttccaa taaagactcc cattcctaaa acagagcctt cacgaataat acagccttca acgacttcag atcgtgcacc aataaaacaa tgatcttcaa taatcgttgg gtttgcttgc agtggttcta aaacacctcc aataccaaca ccaccagaaa gatgaacatg tttgccaatt tgtgcacaag aaccaacagt ggcccatgta tcgaccattg ttccttcatc aacaaaagca ccaaggttga caaaggatgg catcagaata acattgggtg caatataagc agaatggcgt acaatcgctc caggaacgga acggaaatcc gcttttttaa agtcagcttc ttgccaatga gaaaattttg aagggacttt atcccaccaa gatgttccat ttactccacc ggtcataatt tgcataggat tgagccgaaa agaaagaaga acggcttttt tcaaccattg atgaacgtgc cattttccat ttttttgacg ttttacaaca cggacttcac ctttatcaag aaggtttaac gcatgttcga cactctcaag aatttcaccc ttagtggttg tattgataga gttgcggtcg tcaaatgctt tttcaataat catttcaagt tgtgtgagat cagtcatggg agaga
Expressed Protein Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RDGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GACTGATCTC ACACAACTTG AAATGATTAT TGAAAAAGCA TTTGACGACC GCAACTCTAT CAATACAACC ACTAAGGGTG AAATTCTTGA GAGTGTCGAA CATGCGTTAA ACCTTCTTGA TAAAGGTGAA GTCCGTGTTG TAAAACGTCA AAAAAATGGA AAATGGCACG TTCATCAATG GTTGAAAAAA GCCGTTCTTC TTTCTTTTCG GCTCAATCCT ATGCAAATTA TGACCGGTGG AGTAAATGGA ACATCTTGGT GGGATAAAGT CCCTTCAAAA TTTTCTCATT GGCAAGAAGC TGACTTTAAA AAAGCGGATT TCCGTTCCGT TCCTGGAGCG ATTGTACGCC ATTCTGCTTA TATTGCACCC AATGTTATTC TGATGCCATC CTTTGTCAAC CTTGGTGCTT TTGTTGATGA AGGAACAATG GTCGATACAT GGGCCACTGT TGGTTCTTGT GCACAAATTG GCAAACATGT TCATCTTTCT GGTGGTGTTG GTATTGGAGG TGTTTTAGAA CCACTGCAAG CAAACCCAAC GATTATTGAA GATCATTGTT TTATTGGTGC ACGATCTGAA GTCGTTGAAG GCTGTATTAT TCGTGAAGGC TCTGTTTTAG GAATGGGAGT CTTTATTGGA AAATCTACAA AAATTATTGA CCGTACAACA GGTGAAATTT TTATAGGCGA AGTCCCTCCC TATTCTGTCG TTGTTCCAGG TTCTCTTCCC GGAAAACCAC TCCCAAACGG TGAAATAGGC CCTAATCTTT ATTGTGCTGT TATCGTGAAA CGCGTTGACC AAAAAACACG AGAAAAAACA TCTATTAATG ATCTTTTGCG TGACGGGCAC CACCATCATC ATCATTAACG GATCCGAATT CGAGCTCCGT CGACAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG AT
Details for BaheA.00606.a.AE1.PS38600
| PURIFICATION DATe: | 9/12/2019 |
| CONCENTRATION: | 25.74mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RDAGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgtcacgcaa aagatcatta atagatgttt tttctcgtgt tttttggtca acgcgtttca cgataacagc acaataaaga ttagggccta tttcaccgtt tgggagtggt tttccgggaa gagaacctgg aacaacgaca gaatagggag ggacttcgcc tataaaaatt tcacctgttg tacggtcaat aatttttgta gattttccaa taaagactcc cattcctaaa acagagcctt cacgaataat acagccttca acgacttcag atcgtgcacc aataaaacaa tgatcttcaa taatcgttgg gtttgcttgc agtggttcta aaacacctcc aataccaaca ccaccagaaa gatgaacatg tttgccaatt tgtgcacaag aaccaacagt ggcccatgta tcgaccattg ttccttcatc aacaaaagca ccaaggttga caaaggatgg catcagaata acattgggtg caatataagc agaatggcgt acaatcgctc caggaacgga acggaaatcc gcttttttaa agtcagcttc ttgccaatga gaaaattttg aagggacttt atcccaccaa gatgttccat ttactccacc ggtcataatt tgcataggat tgagccgaaa agaaagaaga acggcttttt tcaaccattg atgaacgtgc cattttccat ttttttgacg ttttacaaca cggacttcac ctttatcaag aaggtttaac gcatgttcga cactctcaag aatttcaccc ttagtggttg tattgataga gttgcggtcg tcaaatgctt tttcaataat catttcaagt tgtgtgagat cagtcatggg agaga
Expressed Protein Sequence
MTDLTQLEMI IEKAFDDRNS INTTTKGEIL ESVEHALNLL DKGEVRVVKR QKNGKWHVHQ WLKKAVLLSF RLNPMQIMTG GVNGTSWWDK VPSKFSHWQE ADFKKADFRS VPGAIVRHSA YIAPNVILMP SFVNLGAFVD EGTMVDTWAT VGSCAQIGKH VHLSGGVGIG GVLEPLQANP TIIEDHCFIG ARSEVVEGCI IREGSVLGMG VFIGKSTKII DRTTGEIFIG EVPPYSVVVP GSLPGKPLPN GEIGPNLYCA VIVKRVDQKT REKTSINDLL RDGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GACTGATCTC ACACAACTTG AAATGATTAT TGAAAAAGCA TTTGACGACC GCAACTCTAT CAATACAACC ACTAAGGGTG AAATTCTTGA GAGTGTCGAA CATGCGTTAA ACCTTCTTGA TAAAGGTGAA GTCCGTGTTG TAAAACGTCA AAAAAATGGA AAATGGCACG TTCATCAATG GTTGAAAAAA GCCGTTCTTC TTTCTTTTCG GCTCAATCCT ATGCAAATTA TGACCGGTGG AGTAAATGGA ACATCTTGGT GGGATAAAGT CCCTTCAAAA TTTTCTCATT GGCAAGAAGC TGACTTTAAA AAAGCGGATT TCCGTTCCGT TCCTGGAGCG ATTGTACGCC ATTCTGCTTA TATTGCACCC AATGTTATTC TGATGCCATC CTTTGTCAAC CTTGGTGCTT TTGTTGATGA AGGAACAATG GTCGATACAT GGGCCACTGT TGGTTCTTGT GCACAAATTG GCAAACATGT TCATCTTTCT GGTGGTGTTG GTATTGGAGG TGTTTTAGAA CCACTGCAAG CAAACCCAAC GATTATTGAA GATCATTGTT TTATTGGTGC ACGATCTGAA GTCGTTGAAG GCTGTATTAT TCGTGAAGGC TCTGTTTTAG GAATGGGAGT CTTTATTGGA AAATCTACAA AAATTATTGA CCGTACAACA GGTGAAATTT TTATAGGCGA AGTCCCTCCC TATTCTGTCG TTGTTCCAGG TTCTCTTCCC GGAAAACCAC TCCCAAACGG TGAAATAGGC CCTAATCTTT ATTGTGCTGT TATCGTGAAA CGCGTTGACC AAAAAACACG AGAAAAAACA TCTATTAATG ATCTTTTGCG TGACGGGCAC CACCATCATC ATCATTAACG GATCCGAATT CGAGCTCCGT CGACAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG AT
Details for BaheA.00606.a.A1.PW26985
| PURIFICATION DATe: | 6/1/2009 |
| CONCENTRATION: | 24.62mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLTQLEM IIEKAFDDRN SINTTTKGEI LESVEHALNL LDKGEVRVVK XQKNGKWHVH QWLKKAVLLS FRLNPMQIMT GGVNGTSWWD KVPSKFSHWQ EADFKKADFR SVPGAIVRHS AYIAPNVILM PSFVNLGAFV DEGTMVDTWA TVGSCAQIGK HVHLSGGVGI GGVLEPLQAN PTIIEDHCFI GARSEVVEGC IIREGSVLGM GVFIGKSTKI IDRTTGEIFI GEVPPYSVVV PGSLPGKPLP NGEIGPNLYC AVIVKRVDQK TREKTSINDL LRD
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgactgat ctcacacaac ttgaaatgat tattgaaaaa gcatttgacg accgcaactc tatcaataca accactaagg gtgaaattct tgagagtgtc gaacatgcgt taaaccttct tgataaaggt gaagtccgtg ttgtaaaacn tcaaaaaaat ggaaaatggc acgttcatca atggttgaaa aaagccgttc ttctttcttt tcggctcaat cctatgcaaa ttatgaccgg tggagtaaat ggaacatctt ggtgggataa agtcccttca aaattttctc attggcaaga agctgacttt aaaaaagcgg atttccgttc cgttcctgga gcgattgtac gccattctgc ttatattgca cccaatgtta ttctgatgcc atcctttgtc aaccttggtg cttttgttga tgaaggaaca atggtcgata catgggccac tgttggttct tgtgcacaaa ttggcaaaca tgttcatctt tctggtggtg ttggtattgg aggtgtttta gaaccactgc aagcaaaccc aacgattatt gaagatcatt gttttattgg tgcacgatct gaagtcgttg aaggctgtat tattcgtgaa ggctctgttt taggaatggg agtctttatt ggaaaatcta caaaaattat tgaccgtaca acaggtgaaa tttttatagg cgaagtccct ccctattctg tcgttgttcc aggttctctt cccggaaaac cactcccaaa cggtgaaata ggccctaatc tttattgtgc tgttatcgtg aaacgcgttg accaaaaaac acgagaaaaa acatctatta atgatctttt gcgtgactaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLTQLEM IIEKAFDDRN SINTTTKGEI LESVEHALNL LDKGEVRVVK RQKNGKWHVH QWLKKAVLLS FRLNPMQIMT GGVNGTSWWD KVPSKFSHWQ EADFKKADFR SVPGAIVRHS AYIAPNVILM PSFVNLGAFV DEGTMVDTWA TVGSCAQIGK HVHLSGGVGI GGVLEPLQAN PTIIEDHCFI GARSEVVEGC IIREGSVLGM GVFIGKSTKI IDRTTGEIFI GEVPPYSVVV PGSLPGKPLP NGEIGPNLYC AVIVKRVDQK TREKTSINDL LRD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGATCTC ACACAACTTG AAATGATTAT TGAAAAAGCA TTTGACGACC GCAACTCTAT CAATACAACC ACTAAGGGTG AAATTCTTGA GAGTGTCGAA CATGCGTTAA ACCTTCTTGA TAAAGGTGAA GTCCGTGTTG TAAAACGTCA AAAAAATGGA AAATGGCACG TTCATCAATG GTTGAAAAAA GCCGTTCTTC TTTCTTTTCG GCTCAATCCT ATGCAAATTA TGACCGGTGG AGTAAATGGA ACATCTTGGT GGGATAAAGT CCCTTCAAAA TTTTCTCATT GGCAAGAAGC TGACTTTAAA AAAGCGGATT TCCGTTCCGT TCCTGGAGCG ATTGTACGCC ATTCTGCTTA TATTGCACCC AATGTTATTC TGATGCCATC CTTTGTCAAC CTTGGTGCTT TTGTTGATGA AGGAACAATG GTCGATACAT GGGCCACTGT TGGTTCTTGT GCACAAATTG GCAAACATGT TCATCTTTCT GGTGGTGTTG GTATTGGAGG TGTTTTAGAA CCACTGCAAG CAAACCCAAC GATTATTGAA GATCATTGTT TTATTGGTGC ACGATCTGAA GTCGTTGAAG GCTGTATTAT TCGTGAAGGC TCTGTTTTAG GAATGGGAGT CTTTATTGGA AAATCTACAA AAATTATTGA CCGTACAACA GGTGAAATTT TTATAGGCGA AGTCCCTCCC TATTCTGTCG TTGTTCCAGG TTCTCTTCCC GGAAAACCAC TCCCAAACGG TGAAATAGGC CCTAATCTTT ATTGTGCTGT TATCGTGAAA CGCGTTGACC AAAAAACACG AGAAAAAACA TCTATTAATG ATCTTTTGCG TGACAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA