BaheA.00557.a

ribose-phosphate pyrophosphokinase

CENTER ID: BaheA.00557.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00557.a.A1.GE25787 Full length( BaheA.00557.a ) 1 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1237
RefSeq: YP_033947.1
UniProt: A0A0H3M6B3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLFCGNSNP RLAKEVTNYL SIPLGKATVK RFADQEIFVE LHENVRGQDV FVLQSTSYPA NDHLMELLIM IDALRRSSAR RITAVIPYFG YARQDRKPGP RTPISAKLVA NLITEAGAHR VLTLDLHAGQ IQGFFDIPTD NLYAVPVIAR DVKMHYSLEN VIVVSPDVGG VVRARSLAKR LNSLLAIVDK RRERPGESEV MNIIGDVSGK NCLLLDDIVD SGGTLCNAAS TLLKHGANSV TAYITHGVLS GNAIERITNS EMKELVITNS IMPTEEIEKA HNIRVLPITD LIGEAIARTA AEQSVSSLFG
NT Sequence
ATGAAACTTT TCTGCGGCAA TTCTAATCCA CGCCTTGCTA AAGAAGTTAC AAATTATTTA AGTATTCCCT TAGGCAAAGC AACTGTAAAA CGTTTTGCTG ATCAAGAAAT TTTCGTAGAG TTACATGAAA ATGTACGCGG ACAAGATGTA TTTGTTTTGC AATCTACATC CTATCCAGCA AACGATCATT TAATGGAATT ACTCATCATG ATTGATGCGC TTCGCCGTTC TTCGGCGCGT CGCATCACAG CTGTAATCCC CTATTTTGGT TACGCCCGCC AAGACCGTAA ACCTGGACCA CGAACCCCCA TTTCCGCAAA ACTTGTTGCC AACCTGATCA CTGAAGCAGG AGCCCATCGC GTTTTAACAT TGGACCTTCA TGCAGGACAG ATCCAAGGTT TTTTTGATAT TCCAACGGAT AATCTCTATG CTGTTCCGGT CATTGCTCGC GATGTCAAAA TGCATTATTC CCTTGAAAAT GTTATCGTTG TTTCACCTGA TGTTGGTGGT GTAGTACGAG CACGCTCGCT TGCCAAGCGC TTGAATAGTT TACTAGCTAT TGTTGATAAA CGCCGCGAAC GTCCCGGTGA ATCAGAAGTG ATGAACATCA TTGGAGATGT ATCTGGAAAA AATTGTCTTT TGCTTGATGA TATTGTTGAT TCAGGTGGAA CGCTATGCAA TGCAGCAAGT ACTCTACTAA AGCACGGCGC CAATAGTGTC ACGGCTTACA TCACACACGG TGTTCTTTCT GGAAATGCTA TTGAACGCAT TACCAACTCA GAAATGAAAG AATTAGTTAT TACGAATTCA ATTATGCCGA CAGAAGAAAT TGAGAAAGCC CATAATATCC GTGTTTTGCC AATTACTGAC CTCATTGGAG AAGCAATCGC AAGAACAGCA GCAGAGCAAT CTGTCTCCAG CTTATTCGGT
Details for BaheA.00557.a.A1.GE25787
HARVESTED ON: 10/31/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKLFCGN SNPRLAKEVT NYLSIPLGKA TVKRFADQEI FVELHENVRG QDVFVLQSTS YPANDHLMEL LIMIDALRRS SARRITAVIP YFGYARQDRK PGPRTPISAK LVANLITEAG AHRVLTLDLH AGQIQGFFDI PTDNLYAVPV IARDVKMHYS LENVIVVSPD VGGVVRARSL AKRLNSLLAI VDKRRERPGE SEVMNIIGDV SGKNCLLLDD IVDSGGTLCN AASTLLKHGA NSVTAYITHG VLSGNAIERI TNSEMKELVI TNSIMPTEEI EKAHNIRVLP ITDLIGEAIA RTAAEQSVSS LFG
Validated NT Sequence
gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgaaacttt tctgcggcaa ttctaatcca cgccttgcta aagaagttac aaattattta agtattccct taggcaaagc aactgtaaaa cgttttgctg atcaagaaat tttcgtagag ttacatgaaa atgtacgcgg acaagatgta tttgttttgc aatctacatc ctatccagca aacgatcatt taatggaatt actcatcatg attgatgcgc ttcgccgttc ttcggcgcgt cgcatcacag ctgtaatccc ctattttggt tacgcccgcc aagaccgtaa acctggacca cgaaccccca tttccgcaaa acttgttgcc aacctgatca ctgaagcagg agcccatcgc gttttaacat tggaccttca tgcaggacag atccaaggtt tttttgatat tccaacggat aatctctatg ctgttccggt cattgctcgc gatgtcaaaa tgcattattc ccttgaaaat gttatcgttg tttcacctga tgttggtggt gtagtacgag cacgctcgct tgccaagcgc ttgaatagtt tactagctat tgttgataaa cgccgcgaac gtcccggtga atcagaagtg atgaacatca ttggagatgt atctggaaaa aattgtcttt tgcttgatga tattgttgat tcaggtggaa cgctatgcaa tgcagcaagt actctactaa agcacggcgc caatagtgtc acggcttaca tcacacacgg tgttctttct ggaaatgcta ttgaacgcat taccaactca gaaatgaaag aattagttat tacgaattca attatgccga cagaagaaat tgagaaagcc cataatatcc gtgttttgcc aattactgac ctcattggag aagcaatcgc aagaacagca gcggagcaat ctgtctccag cttattcggt taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLFCGNSN PRLAKEVTNY LSIPLGKATV KRFADQEIFV ELHENVRGQD VFVLQSTSYP ANDHLMELLI MIDALRRSSA RRITAVIPYF GYARQDRKPG PRTPISAKLV ANLITEAGAH RVLTLDLHAG QIQGFFDIPT DNLYAVPVIA RDVKMHYSLE NVIVVSPDVG GVVRARSLAK RLNSLLAIVD KRRERPGESE VMNIIGDVSG KNCLLLDDIV DSGGTLCNAA STLLKHGANS VTAYITHGVL SGNAIERITN SEMKELVITN SIMPTEEIEK AHNIRVLPIT DLIGEAIART AAEQSVSSLF G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacttttc tgcggcaatt ctaatccacg ccttgctaaa gaagttacaa attatttaag tattccctta ggcaaagcaa ctgtaaaacg ttttgctgat caagaaattt tcgtagagtt acatgaaaat gtacgcggac aagatgtatt tgttttgcaa tctacatcct atccagcaaa cgatcattta atggaattac tcatcatgat tgatgcgctt cgccgttctt cggcgcgtcg catcacagct gtaatcccct attttggtta cgcccgccaa gaccgtaaac ctggaccacg aacccccatt tccgcaaaac ttgttgccaa cctgatcact gaagcaggag cccatcgcgt tttaacattg gaccttcatg caggacagat ccaaggtttt tttgatattc caacggataa tctctatgct gttccggtca ttgctcgcga tgtcaaaatg cattattccc ttgaaaatgt tatcgttgtt tcacctgatg ttggtggtgt agtacgagca cgctcgcttg ccaagcgctt gaatagttta ctagctattg ttgataaacg ccgcgaacgt cccggtgaat cagaagtgat gaacatcatt ggagatgtat ctggaaaaaa ttgtcttttg cttgatgata ttgttgattc aggtggaacg ctatgcaatg cagcaagtac tctactaaag cacggcgcca atagtgtcac ggcttacatc acacacggtg ttctttctgg aaatgctatt gaacgcatta ccaactcaga aatgaaagaa ttagttatta cgaattcaat tatgccgaca gaagaaattg agaaagccca taatatccgt gttttgccaa ttactgacct cattggagaa gcaatcgcaa gaacagcagc agagcaatct gtctccagct tattcggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa