BaheA.00545.a

threonine synthase

CENTER ID: BaheA.00545.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00545.a.A1.PW25847 Full length( BaheA.00545.a ) 1 465

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.489
RefSeq: YP_033286.1
UniProt: A0A0H3LW18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQYISTRGEA PALGFIETIM TGLASDGGLY LPEKFPQLSF DALRALRGQS YMTIAKTILW PFVNNEIEYT SFENMISESY ATFHHPATCP LLQTGPNEFI LELFHGPTLA FKDVAMQFLS RLMDYVLIKK NKSATIIAAT SGDTGGAAIQ AFAGREYSDV FILFPKGRIS PVQQRQMTTS QKTNVHAIAI EGSFDDCQAL VKAMFNDHNF RQKKALSGVN SINWARIMAQ IVYYFSSALS LGAPDRAVSF TVPTGNFGDI FAGYVAARMG LPIAQLVIAT NDNDILVRTL TNGTYETQPT THTTSPSMDI QVSSNFERLL FESGDRDPVW IRNAMENLKK SGYFNLNEKQ LKNIRSLFSA GKSSIAETAK IIDSVYKESG YLVDPHTAVA LKVARENRQP HIPMIVLSTA HPAKFPDAIK NACGLNAPWP SRLNDLIERE EHFIGLANDE KIVKDYISLK SRASY
NT Sequence
ATGCAATATA TCAGCACGAG AGGTGAGGCT CCTGCTTTAG GTTTTATTGA AACTATCATG ACTGGACTGG CTAGTGATGG TGGACTTTAT CTTCCAGAAA AATTCCCCCA ATTATCATTC GATGCATTAC GAGCGCTTCG TGGCCAATCC TATATGACAA TCGCTAAAAC TATTCTTTGG CCCTTTGTGA ATAACGAAAT TGAATATACT TCTTTTGAAA ATATGATTTC TGAATCTTAT GCTACCTTCC ATCATCCTGC AACCTGTCCG TTACTGCAAA CTGGCCCAAA TGAATTTATT CTTGAACTTT TTCACGGTCC TACTTTGGCA TTTAAAGATG TTGCAATGCA GTTTCTTTCT CGACTGATGG ATTATGTGCT CATCAAAAAA AATAAATCCG CAACAATTAT TGCTGCTACA TCAGGTGATA CAGGTGGTGC CGCAATACAA GCTTTTGCTG GAAGAGAATA TTCAGACGTT TTTATCTTGT TTCCTAAAGG GCGTATATCA CCCGTTCAGC AGCGGCAAAT GACGACCAGT CAAAAAACAA ACGTGCATGC CATAGCCATT GAAGGGAGTT TTGATGATTG CCAAGCACTG GTGAAAGCAA TGTTTAATGA CCATAATTTC CGGCAAAAAA AAGCACTTTC AGGAGTTAAT TCTATAAATT GGGCGCGTAT TATGGCGCAA ATTGTTTATT ATTTTTCATC CGCACTTTCT TTAGGTGCTC CTGATAGAGC TGTCTCATTT ACTGTTCCTA CTGGAAATTT TGGTGATATT TTTGCGGGTT ATGTTGCTGC CCGTATGGGA TTACCCATTG CCCAATTGGT CATTGCAACA AATGATAATG ATATCCTCGT GCGCACATTG ACTAATGGTA CTTATGAAAC ACAGCCGACA ACCCACACAA CGTCACCCTC TATGGATATT CAAGTCTCTT CTAATTTTGA ACGCTTACTG TTTGAAAGTG GCGATCGTGA TCCTGTGTGG ATTCGCAATG CGATGGAAAA CTTGAAAAAG TCAGGATATT TTAATCTTAA TGAAAAGCAG CTCAAAAATA TTCGTTCCCT CTTTTCTGCA GGGAAAAGTA GCATAGCTGA AACGGCAAAA ATAATTGACA GTGTTTATAA GGAAAGCGGC TATCTCGTTG ATCCACATAC AGCTGTTGCG CTTAAAGTAG CCCGTGAAAA CAGACAACCA CATATTCCAA TGATCGTCCT CTCTACTGCT CATCCTGCTA AATTTCCTGA TGCCATCAAA AATGCCTGTG GACTTAATGC TCCATGGCCA TCTCGTCTCA ACGATTTAAT AGAACGCGAA GAACATTTTA TAGGTCTTGC TAATGATGAA AAAATAGTAA AAGATTACAT TTCTTTAAAA TCGCGTGCAT CTTAT
Details for BaheA.00545.a.A1.PW25847
PURIFICATION DATe: 1/28/2009
CONCENTRATION: 33.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MQYISTRGEA PALGFIETIM TGXASDGGLY LPEKFPQLSF DALRALRGQS YMTIAKTILW PFVNNEIEYT SFENMISESY ATFHHPATCP LXQTGPNEFI LELFHGPTLA FKDVAMQFLS RLMDYVLIKK NKSATIIAAT SGDTGGAAIQ AFAGXXYSDV FILFXKGXIS XVQQRQXXXX QKTNVHAIXX XXSFDDXXXX VKAXXXDHNF RQKKALSGXN SXNWARIMAQ IVYYFSXALS LGXXDRAVSF TVXTGNFGDI FAGYVAARMG LPIAQLVIAT NDNDIXVRTL TNGTYETQPT THTTSPSMDI QVSSNFERLL FESGDRDPVW IRNAMENLKK SGYFNLNEKQ LKNIRSLFSA GKSSIAETAK IIDSVYKESG YLVDPHTAVA LKVARENRQP HIPMIVLSTA HPAKFPDAIK NACGLNAPXX XRLNDLIERE EHFIGLAXDE KIVKDYISLK SRAS
Validated NT Sequence
ataagatgca cgcgatttta aagaaatgta atcttttact attttttcat cannagcaag acctataaaa tgttcttcgc gttctattaa atcgttgaga cgagnnnnnc atggagcatt aagtccacag gcatttttga tggcatcagg aaatttagca ggatgagcag tagagaggac gatcattgga atatgtggtt gtctgttttc acgggctact ttaagcgcaa cagctgtatg tggatcaacg agatagccgc tttccttata aacactgtca attatttttg ccgtttcagc tatgctactt ttccctgcag aaaagaggga acgaatattt ttgagctgct tttcattaag attaaaatat cctgactttt tcaagttttc catcgcattg cgaatccaca caggatcacg atcgccactt tcaaacagta agcgttcaaa attagaagag acttgaatat ccatagaggg tgacgttgtg tgggttgtcg gctgtgtttc ataagtacca ttagtcaatg tgcgcacgan gatatcatta tcatttgttg caatgaccaa ttgggcaatg ggtaatccca tacgggcagc aacatanccc gcaaaaatat caccaaaatt tccagtanga acagtaaatg agacagctct atcannanca cctaangaaa gtgcgnatga aaaataataa acaatttgcg ccataatacg cgcccaattn atagaattaa ntcctgaaag tgcttttttt tgncggaaat tatggtcnnt nannnttgct ttcaccnnng ntnngcnatc atcaaaactn nnnnnnntgn ntatggcatg cacgtttgtt ttttgacngn tcntcntttg ccgctgctga acggntgata tnnnnccttt angaaacaag ataaanacgt cngaatatnn nnttccagca aaagcttgta ttgcggcacc acctgtatca cctgatgtag cagcaataat tgttgcggat ttattttttt tgatgagcac ataatccatc agtcgagaaa gaaactgcat tgcaacatct ttaaatgcca aagtaggacc gtgaaaaagt tcaagaataa attcatttgg gccagtttgc ngtaacggac aggttgcagg atgatggaag gtagcataag attcagaaat catattttca aaagaagtat attcaatttc gttattcaca aagggccaaa gaatagtttt agcgattgtc atataggatt ggccacgaag cgctcgtaat gcatcgaatg ataattgggg gaatttttct ggaagataaa gtccaccatc actagccann ccagtcatga tagtttcaat aaaacctaaa gcaggagcct cacctctcgt gctgatatat tgcatcgaac caggaccctg ggtctgagct tccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYISTRGE APALGFIETI MTGLASDGGL YLPEKFPQLS FDALRALRGQ SYMTIAKTIL WPFVNNEIEY TSFENMISES YATFHHPATC PLLQTGPNEF ILELFHGPTL AFKDVAMQFL SRLMDYVLIK KNKSATIIAA TSGDTGGAAI QAFAGREYSD VFILFPKGRI SPVQQRQMTT SQKTNVHAIA IEGSFDDCQA LVKAMFNDHN FRQKKALSGV NSINWARIMA QIVYYFSSAL SLGAPDRAVS FTVPTGNFGD IFAGYVAARM GLPIAQLVIA TNDNDILVRT LTNGTYETQP TTHTTSPSMD IQVSSNFERL LFESGDRDPV WIRNAMENLK KSGYFNLNEK QLKNIRSLFS AGKSSIAETA KIIDSVYKES GYLVDPHTAV ALKVARENRQ PHIPMIVLST AHPAKFPDAI KNACGLNAPW PSRLNDLIER EEHFIGLAND EKIVKDYISL KSRASY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATATATC AGCACGAGAG GTGAGGCTCC TGCTTTAGGT TTTATTGAAA CTATCATGAC TGGACTGGCT AGTGATGGTG GACTTTATCT TCCAGAAAAA TTCCCCCAAT TATCATTCGA TGCATTACGA GCGCTTCGTG GCCAATCCTA TATGACAATC GCTAAAACTA TTCTTTGGCC CTTTGTGAAT AACGAAATTG AATATACTTC TTTTGAAAAT ATGATTTCTG AATCTTATGC TACCTTCCAT CATCCTGCAA CCTGTCCGTT ACTGCAAACT GGCCCAAATG AATTTATTCT TGAACTTTTT CACGGTCCTA CTTTGGCATT TAAAGATGTT GCAATGCAGT TTCTTTCTCG ACTGATGGAT TATGTGCTCA TCAAAAAAAA TAAATCCGCA ACAATTATTG CTGCTACATC AGGTGATACA GGTGGTGCCG CAATACAAGC TTTTGCTGGA AGAGAATATT CAGACGTTTT TATCTTGTTT CCTAAAGGGC GTATATCACC CGTTCAGCAG CGGCAAATGA CGACCAGTCA AAAAACAAAC GTGCATGCCA TAGCCATTGA AGGGAGTTTT GATGATTGCC AAGCACTGGT GAAAGCAATG TTTAATGACC ATAATTTCCG GCAAAAAAAA GCACTTTCAG GAGTTAATTC TATAAATTGG GCGCGTATTA TGGCGCAAAT TGTTTATTAT TTTTCATCCG CACTTTCTTT AGGTGCTCCT GATAGAGCTG TCTCATTTAC TGTTCCTACT GGAAATTTTG GTGATATTTT TGCGGGTTAT GTTGCTGCCC GTATGGGATT ACCCATTGCC CAATTGGTCA TTGCAACAAA TGATAATGAT ATCCTCGTGC GCACATTGAC TAATGGTACT TATGAAACAC AGCCGACAAC CCACACAACG TCACCCTCTA TGGATATTCA AGTCTCTTCT AATTTTGAAC GCTTACTGTT TGAAAGTGGC GATCGTGATC CTGTGTGGAT TCGCAATGCG ATGGAAAACT TGAAAAAGTC AGGATATTTT AATCTTAATG AAAAGCAGCT CAAAAATATT CGTTCCCTCT TTTCTGCAGG GAAAAGTAGC ATAGCTGAAA CGGCAAAAAT AATTGACAGT GTTTATAAGG AAAGCGGCTA TCTCGTTGAT CCACATACAG CTGTTGCGCT TAAAGTAGCC CGTGAAAACA GACAACCACA TATTCCAATG ATCGTCCTCT CTACTGCTCA TCCTGCTAAA TTTCCTGATG CCATCAAAAA TGCCTGTGGA CTTAATGCTC CATGGCCATC TCGTCTCAAC GATTTAATAG AACGCGAAGA ACATTTTATA GGTCTTGCTA ATGATGAAAA AATAGTAAAA GATTACATTT CTTTAAAATC GCGTGCATCT TATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA