BaheA.00498.a
Pantoate-beta-alanine ligase
| CENTER ID: | BaheA.00498.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00498.a.B1.GE43953 | Full length( BaheA.00498.a ) | 1 | 286 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00498.a.B1.PW38921 | Full length( BaheA.00498.a ) | 1 | 286 |
Structures
7TOT
DEPOSITED: 1/24/2022
DETERMINATION: XRay
CLONE: BaheA.00498.a.B1.GE43953
PROTEIN: BaheA.00498.a.B1.PW38921
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129569 |
| PATRIC ID: | fig|283166.5.peg.556 |
| RefSeq: | YP_033348.1 |
| UniProt: | Q6G456 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKMRVLKTIP EVRQSITEER RLGFSIGLVP TMGALHNGHI ALVRRARAMC DRVLVSIFVN PKQFGPDEDF DKYPRDLKGD CALLEEAGVE YLFTPSVEEM WPPGNETIVN VEKLSRMLIG KLRPGHFCGV TSVVAKLFNI VQPDKAFFGE KDFQQILIVR RMVEDLAFPI EVVGVPVLRE ADGVASSSRN QFLTLEDRKA AKIIPESGKA AENLYRQGER SVDKLCKIVR DILQQESRAI IESIDLRDME TLSVVKGRLD KSAVLLLTVR FGEIRLIDQY ILQEKG
NT Sequence
ATGAAGATGA GAGTCTTAAA AACAATTCCC GAAGTACGCC AATCTATCAC AGAAGAACGA CGTTTAGGAT TTTCGATTGG TTTGGTTCCA ACTATGGGAG CATTGCATAA TGGTCACATC GCATTAGTAC GGCGAGCAAG GGCAATGTGT GATCGAGTAT TGGTTTCTAT TTTTGTCAAT CCGAAGCAAT TTGGTCCCGA TGAGGATTTT GATAAATATC CAAGAGATTT GAAAGGCGAT TGTGCTTTGT TAGAAGAAGC CGGTGTTGAA TATCTTTTTA CACCTTCTGT AGAAGAAATG TGGCCACCGG GAAATGAGAC AATTGTGAAT GTAGAGAAAT TGTCACGTAT GTTGATAGGG AAATTACGTC CAGGACATTT TTGTGGTGTG ACCAGCGTTG TTGCGAAACT TTTTAACATC GTTCAGCCGG ATAAAGCTTT TTTTGGGGAA AAGGATTTTC AACAGATTTT GATTGTTCGT CGTATGGTTG AAGACTTAGC TTTTCCTATT GAAGTTGTAG GAGTGCCTGT TTTACGTGAA GCAGATGGTG TAGCGAGTTC TTCCCGTAAT CAGTTTTTAA CATTAGAAGA TCGAAAAGCG GCAAAAATTA TTCCTGAGAG CGGTAAAGCT GCTGAAAATC TCTATCGCCA AGGGGAACGG TCGGTTGATA AATTATGTAA AATTGTTCGG GATATTTTAC AGCAAGAATC GCGCGCGATT ATTGAATCAA TAGACTTACG GGATATGGAA ACTTTATCTG TGGTTAAGGG GAGATTGGAT AAATCGGCAG TTTTATTATT AACTGTCCGT TTTGGTGAAA TTAGGCTTAT TGATCAATAC ATATTGCAAG AAAAGGGC
Details for BaheA.00498.a.B1.GE43953
| HARVESTED ON: | 12/3/2020 |
| SEQUENCED ON: | 12/16/2020 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 4 |
Validated AA Sequence
MAHHHHHHMK MRV
Validated NT Sequence
atggctcacc accaccacca ccatatgaag atgagagtct aaaaannaat tcccgaagta cgccaatcta tcacagaana ncnacgttta ggatttcgat tggtttggtt ccaactatgg gagcattgca taatggtcac atcgcattag tacggcgagc aagggcaatg tgtgatcgag tattggtttc tattttttgt caatcngaag caatttggtc ccgatgagga ttttgataaa tatccaagag atttgaaagg cgattgtgct ttgttagaag aagccggtgt tgaatatctt tttacncctt ctgtagaaga aatgtggcca ccgggaaatg agacaattgt gaatgtagag aaattgtcac gtatgttgat agggaaatta cgtccaggac atttttgtgg tgtgaccagc gttgttgcga aactttttaa catcgttcag ccggataaag ctttttttgg ggaaaaggat tttcaacaga ttttgattgt tcgtcgtatg gttgaagact tagcttttcc tattgaagtt gtaggagtgc ctgttttacg tgaagcagat ggtgtagcga gttcttcccg taatcagttt ttaacattag aagatcgaaa agcggcaaaa attattcctg agagcggtaa agctgctgaa aatctctatc gccnngngga acggtcggtt gataaattat gtnnaattgt tcgggatatt ttacagcaag aatcgcgcgc gattattgaa tcaatagact tacgggatat ggaaacttta tctgtggtta aggggagatt ggataaatcg gcagttttat tattanctgt ccgttttggt gaaattaggc ttattgatca atacatattg ca
Expected Protein Sequence
MAHHHHHHMK MRVLKTIPEV RQSITEERRL GFSIGLVPTM GALHNGHIAL VRRARAMCDR VLVSIFVNPK QFGPDEDFDK YPRDLKGDCA LLEEAGVEYL FTPSVEEMWP PGNETIVNVE KLSRMLIGKL RPGHFCGVTS VVAKLFNIVQ PDKAFFGEKD FQQILIVRRM VEDLAFPIEV VGVPVLREAD GVASSSRNQF LTLEDRKAAK IIPESGKAAE NLYRQGERSV DKLCKIVRDI LQQESRAIIE SIDLRDMETL SVVKGRLDKS AVLLLTVRFG EIRLIDQYIL QEKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga tgagagtctt aaaaacaatt cccgaagtac gccaatctat cacagaagaa cgacgtttag gattttcgat tggtttggtt ccaactatgg gagcattgca taatggtcac atcgcattag tacggcgagc aagggcaatg tgtgatcgag tattggtttc tatttttgtc aatccgaagc aatttggtcc cgatgaggat tttgataaat atccaagaga tttgaaaggc gattgtgctt tgttagaaga agccggtgtt gaatatcttt ttacaccttc tgtagaagaa atgtggccac cgggaaatga gacaattgtg aatgtagaga aattgtcacg tatgttgata gggaaattac gtccaggaca tttttgtggt gtgaccagcg ttgttgcgaa actttttaac atcgttcagc cggataaagc tttttttggg gaaaaggatt ttcaacagat tttgattgtt cgtcgtatgg ttgaagactt agcttttcct attgaagttg taggagtgcc tgttttacgt gaagcagatg gtgtagcgag ttcttcccgt aatcagtttt taacattaga agatcgaaaa gcggcaaaaa ttattcctga gagcggtaaa gctgctgaaa atctctatcg ccaaggggaa cggtcggttg ataaattatg taaaattgtt cgggatattt tacagcaaga atcgcgcgcg attattgaat caatagactt acgggatatg gaaactttat ctgtggttaa ggggagattg gataaatcgg cagttttatt attaactgtc cgttttggtg aaattaggct tattgatcaa tacatattgc aagaaaaggg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BaheA.00498.a.B1.PW38921
| PURIFICATION DATe: | 1/11/2021 |
| CONCENTRATION: | 21mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 4 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK MRV
Validated NT Sequence
atggctcacc accaccacca ccatatgaag atgagagtct aaaaannaat tcccgaagta cgccaatcta tcacagaana ncnacgttta ggatttcgat tggtttggtt ccaactatgg gagcattgca taatggtcac atcgcattag tacggcgagc aagggcaatg tgtgatcgag tattggtttc tattttttgt caatcngaag caatttggtc ccgatgagga ttttgataaa tatccaagag atttgaaagg cgattgtgct ttgttagaag aagccggtgt tgaatatctt tttacncctt ctgtagaaga aatgtggcca ccgggaaatg agacaattgt gaatgtagag aaattgtcac gtatgttgat agggaaatta cgtccaggac atttttgtgg tgtgaccagc gttgttgcga aactttttaa catcgttcag ccggataaag ctttttttgg ggaaaaggat tttcaacaga ttttgattgt tcgtcgtatg gttgaagact tagcttttcc tattgaagtt gtaggagtgc ctgttttacg tgaagcagat ggtgtagcga gttcttcccg taatcagttt ttaacattag aagatcgaaa agcggcaaaa attattcctg agagcggtaa agctgctgaa aatctctatc gccnngngga acggtcggtt gataaattat gtnnaattgt tcgggatatt ttacagcaag aatcgcgcgc gattattgaa tcaatagact tacgggatat ggaaacttta tctgtggtta aggggagatt ggataaatcg gcagttttat tattanctgt ccgttttggt gaaattaggc ttattgatca atacatattg ca
Expressed Protein Sequence
MAHHHHHHMK MRVLKTIPEV RQSITEERRL GFSIGLVPTM GALHNGHIAL VRRARAMCDR VLVSIFVNPK QFGPDEDFDK YPRDLKGDCA LLEEAGVEYL FTPSVEEMWP PGNETIVNVE KLSRMLIGKL RPGHFCGVTS VVAKLFNIVQ PDKAFFGEKD FQQILIVRRM VEDLAFPIEV VGVPVLREAD GVASSSRNQF LTLEDRKAAK IIPESGKAAE NLYRQGERSV DKLCKIVRDI LQQESRAIIE SIDLRDMETL SVVKGRLDKS AVLLLTVRFG EIRLIDQYIL QEKG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA TGAGAGTCTT AAAAACAATT CCCGAAGTAC GCCAATCTAT CACAGAAGAA CGACGTTTAG GATTTTCGAT TGGTTTGGTT CCAACTATGG GAGCATTGCA TAATGGTCAC ATCGCATTAG TACGGCGAGC AAGGGCAATG TGTGATCGAG TATTGGTTTC TATTTTTGTC AATCCGAAGC AATTTGGTCC CGATGAGGAT TTTGATAAAT ATCCAAGAGA TTTGAAAGGC GATTGTGCTT TGTTAGAAGA AGCCGGTGTT GAATATCTTT TTACACCTTC TGTAGAAGAA ATGTGGCCAC CGGGAAATGA GACAATTGTG AATGTAGAGA AATTGTCACG TATGTTGATA GGGAAATTAC GTCCAGGACA TTTTTGTGGT GTGACCAGCG TTGTTGCGAA ACTTTTTAAC ATCGTTCAGC CGGATAAAGC TTTTTTTGGG GAAAAGGATT TTCAACAGAT TTTGATTGTT CGTCGTATGG TTGAAGACTT AGCTTTTCCT ATTGAAGTTG TAGGAGTGCC TGTTTTACGT GAAGCAGATG GTGTAGCGAG TTCTTCCCGT AATCAGTTTT TAACATTAGA AGATCGAAAA GCGGCAAAAA TTATTCCTGA GAGCGGTAAA GCTGCTGAAA ATCTCTATCG CCAAGGGGAA CGGTCGGTTG ATAAATTATG TAAAATTGTT CGGGATATTT TACAGCAAGA ATCGCGCGCG ATTATTGAAT CAATAGACTT ACGGGATATG GAAACTTTAT CTGTGGTTAA GGGGAGATTG GATAAATCGG CAGTTTTATT ATTAACTGTC CGTTTTGGTG AAATTAGGCT TATTGATCAA TACATATTGC AAGAAAAGGG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT