BaheA.00339.a
Alanine racemase
| CENTER ID: | BaheA.00339.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00339.a.A1.GE25763 | Full length( BaheA.00339.a ) | 1 | 372 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00339.a.A1.PS00157 | Full length( BaheA.00339.a ) | 1 | 372 | ||
| BaheA.00339.a.A1.PW27468 | Full length( BaheA.00339.a ) | 1 | 372 |
Structures
3KW3
DEPOSITED: 11/30/2009
DETERMINATION: XRay
CLONE: BaheA.00339.a.A1.GE25763
PROTEIN: BaheA.00339.a.A1.PS00157
Publications by SSGCID
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PMID: 21359836; PMCID: PMC3123459
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131271 |
| PATRIC ID: | fig|283166.5.peg.1362 |
| RefSeq: | YP_034019.1 |
| UniProt: | A0A0H3LZE4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKSAKDTAN PLFPATAIAT IDVRAIVANY RTLAQHVAPT ECSAVVKANA YGLGAHKIAP ALYQAGCRTF FVAQIEEALQ LKAVLPENVM IALLNGFPHK AEEFVAQSGI IPLLNSWSTI EDWQTLCQKK NKKFPAIIQV DTNMSRLGLD KKELQKLIKN PTIFEKAEIK YILSHLANGE DASHSSNNKQ LAAFKRVLAQ LPTCKVSFAN SGGIFLGSDF YFDLVRPGIA LYGVDPHGKH PTPLKAVVKV EAQVLQSRFI DAGIPVGYRE SFMTRRPSTL ATISIGYADG WPRILSNKGT VYFNGHKLPI VGHISMDSII VDATDLDKKP QRGDWVELIG PHQPLEKVST DTNTIPHEIL TSLGKRYKRI YI
NT Sequence
ATGAACAAAT CCGCTAAGGA TACAGCAAAC CCATTATTTC CTGCTACAGC TATAGCAACC ATCGACGTGC GTGCTATTGT TGCAAATTAT AGAACTTTAG CCCAACATGT TGCTCCAACT GAGTGTTCAG CGGTAGTGAA AGCTAATGCC TATGGGTTAG GGGCACATAA AATTGCCCCG GCACTTTATC AAGCTGGTTG CCGCACTTTT TTTGTTGCCC AAATCGAAGA AGCGCTTCAA TTAAAAGCCG TTTTACCAGA AAATGTCATG ATTGCTCTTC TGAATGGTTT TCCACATAAA GCGGAAGAAT TTGTCGCACA ATCGGGTATT ATCCCTCTTT TGAACTCTTG GTCTACTATC GAAGATTGGC AAACACTTTG CCAAAAAAAG AATAAAAAAT TTCCCGCAAT CATTCAAGTC GATACCAATA TGAGCCGATT AGGGCTTGAT AAAAAAGAAT TACAAAAACT CATCAAAAAC CCTACAATTT TTGAAAAAGC AGAAATAAAA TATATTCTCA GTCACCTTGC GAATGGAGAG GACGCCTCTC ATTCATCCAA TAATAAACAA TTAGCTGCTT TCAAGAGAGT ACTTGCACAG TTACCTACCT GTAAAGTCTC ATTTGCCAAT TCTGGAGGCA TTTTTCTTGG TTCAGATTTT TATTTTGATC TTGTTCGCCC CGGTATTGCA CTTTACGGTG TTGATCCCCA TGGCAAACAT CCAACACCTC TTAAAGCTGT TGTAAAAGTT GAAGCCCAAG TTCTTCAAAG CCGCTTTATT GACGCAGGAA TACCTGTTGG TTATCGAGAA AGCTTTATGA CCCGCAGACC AAGCACTCTT GCAACCATTT CTATTGGCTA TGCAGATGGT TGGCCACGGA TTCTTTCGAA TAAAGGTACC GTTTATTTCA ATGGGCACAA ACTCCCTATT GTTGGACATA TTTCTATGGA TTCTATCATA GTCGATGCTA CTGATCTCGA TAAAAAACCT CAAAGAGGTG ATTGGGTAGA GCTCATTGGC CCTCATCAAC CCCTTGAAAA AGTATCTACA GATACCAATA CCATTCCTCA TGAAATTCTC ACTTCTCTAG GAAAACGCTA TAAGCGCATT TACATT
Details for BaheA.00339.a.A1.GE25763
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNKSAKDTA NPLFPATAIA TIDVRAIVAN YRTLAQHVAP TECSAVVKAN AYGLGAHKIA PALYQAGCRT FFVAQIEEAL QLKAVLPENV MIALLNGFPH KAEEFVAQSG IIPLLNSWST IEDWQTLCQK KNKKFPAIIQ VDTNMSRLGL DKKELQKLIK NPTIFEKAEI KYILSHLANG EDASHSSNNK QLAAFKRVLA QLPTCKVSFA NSGGIFLGSD FYFDLVRPGI ALYGVDPHGK HPTPLKAVVK VEAQVLQSRF IDAGIPVGYR ESFMTRRPST LATISIGYAD GWPRILSNKG TVYFNGHKLP IVGHISMDSI IVDATDLDKK PQRGDWVELI GPHQPLEKVS TDTNTIPHEI LTSLGKRYKR I
Validated NT Sequence
aaatgcgctt atagcgtttt cctagagaag tgagaatttc atgaggaatg gtattggtat ctgtagatac tttttcaagg ggttgatgag ggccaatgag ctctacccaa tcacctcttt gaggtttttt atcgagatca gtagcatcga ctatgataga atccatagaa atatgtccaa caatagggag tttgtgccca ttgaaataaa cggtaccttt attcgaaaga atccgtggcc aaccatctgc atagccaata gaaatggttg caagagtgct tggtctgcgg gtcataaagc tttctcgata accaacaggt attcctgcgt caataaagcg gctttgaaga acttgggctt caacttttac aacagcttta agaggtgttg gatgtttgcc atggggatca acaccgtaaa gtgcaatacc ggggcgaaca agatcaaaat aaaaatctga accaagaaaa atgcctccag aattggcaaa tgagacttta caggtaggta actgtgcaag tactctcttg aaagcagcta attgtttatt attggatgaa tgagaggcgt cctctccatt cgcaaggtga ctgagaatat attttatttc tgctttttca aaaattgtag ggtttttgat gagtttttgt aattcttttt tatcaagccc taatcggctc atattggtat cgacttgaat gattgcggga aattttttat tctttttttg gcaaagtgtt tgccaatctt cgatagtaga ccaagagttc aaaagaggga taatacccga ttgtgcgaca aattcttccg ctttatgtgg aaaaccattc agaagagcaa tcatgacatt ttctggtaaa acggctttta attgaagcgc ttcttcgatt tgggcaacaa aaaaagtgcg gcaaccagct tgataaagtg ccggggcaat tttatgtgcc cctaacccat aggcattagc tttcactacc gctgaacact cagttggagc aacatgttgg gctaaagttc tataatttgc aacaatagca cgcacgtcga tggttgctat agctgtagca ggaaataatg ggtttgctgt atccttagcg gatttgttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNKSAKDTA NPLFPATAIA TIDVRAIVAN YRTLAQHVAP TECSAVVKAN AYGLGAHKIA PALYQAGCRT FFVAQIEEAL QLKAVLPENV MIALLNGFPH KAEEFVAQSG IIPLLNSWST IEDWQTLCQK KNKKFPAIIQ VDTNMSRLGL DKKELQKLIK NPTIFEKAEI KYILSHLANG EDASHSSNNK QLAAFKRVLA QLPTCKVSFA NSGGIFLGSD FYFDLVRPGI ALYGVDPHGK HPTPLKAVVK VEAQVLQSRF IDAGIPVGYR ESFMTRRPST LATISIGYAD GWPRILSNKG TVYFNGHKLP IVGHISMDSI IVDATDLDKK PQRGDWVELI GPHQPLEKVS TDTNTIPHEI LTSLGKRYKR IYI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaaatcc gctaaggata cagcaaaccc attatttcct gctacagcta tagcaaccat cgacgtgcgt gctattgttg caaattatag aactttagcc caacatgttg ctccaactga gtgttcagcg gtagtgaaag ctaatgccta tgggttaggg gcacataaaa ttgccccggc actttatcaa gctggttgcc gcactttttt tgttgcccaa atcgaagaag cgcttcaatt aaaagccgtt ttaccagaaa atgtcatgat tgctcttctg aatggttttc cacataaagc ggaagaattt gtcgcacaat cgggtattat ccctcttttg aactcttggt ctactatcga agattggcaa acactttgcc aaaaaaagaa taaaaaattt cccgcaatca ttcaagtcga taccaatatg agccgattag ggcttgataa aaaagaatta caaaaactca tcaaaaaccc tacaattttt gaaaaagcag aaataaaata tattctcagt caccttgcga atggagagga cgcctctcat tcatccaata ataaacaatt agctgctttc aagagagtac ttgcacagtt acctacctgt aaagtctcat ttgccaattc tggaggcatt tttcttggtt cagattttta ttttgatctt gttcgccccg gtattgcact ttacggtgtt gatccccatg gcaaacatcc aacacctctt aaagctgttg taaaagttga agcccaagtt cttcaaagcc gctttattga cgcaggaata cctgttggtt atcgagaaag ctttatgacc cgcagaccaa gcactcttgc aaccatttct attggctatg cagatggttg gccacggatt ctttcgaata aaggtaccgt ttatttcaat gggcacaaac tccctattgt tggacatatt tctatggatt ctatcatagt cgatgctact gatctcgata aaaaacctca aagaggtgat tgggtagagc tcattggccc tcatcaaccc cttgaaaaag tatctacaga taccaatacc attcctcatg aaattctcac ttctctagga aaacgctata agcgcattta cattaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.00339.a.A1.PS00157
| PURIFICATION DATe: | 2/4/2009 |
| CONCENTRATION: | 42.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNKSAKDTA NPLFPATAIA TIDVRAIVAN YRTLAQHVAP TECSAVVKAN AYGLGAHKIA PALYQAGCRT FFVAQIEEAL QLKAVLPENV MIALLNGFPH KAEEFVAQSG IIPLLNSWST IEDWQTLCQK KNKKFPAIIQ VDTNMSRLGL DKKELQKLIK NPTIFEKAEI KYILSHLANG EDASHSSNNK QLAAFKRVLA QLPTCKVSFA NSGGIFLGSD FYFDLVRPGI ALYGVDPHGK HPTPLKAVVK VEAQVLQSRF IDAGIPVGYR ESFMTRRPST LATISIGYAD GWPRILSNKG TVYFNGHKLP IVGHISMDSI IVDATDLDKK PQRGDWVELI GPHQPLEKVS TDTNTIPHEI LTSLGKRYKR I
Validated NT Sequence
aaatgcgctt atagcgtttt cctagagaag tgagaatttc atgaggaatg gtattggtat ctgtagatac tttttcaagg ggttgatgag ggccaatgag ctctacccaa tcacctcttt gaggtttttt atcgagatca gtagcatcga ctatgataga atccatagaa atatgtccaa caatagggag tttgtgccca ttgaaataaa cggtaccttt attcgaaaga atccgtggcc aaccatctgc atagccaata gaaatggttg caagagtgct tggtctgcgg gtcataaagc tttctcgata accaacaggt attcctgcgt caataaagcg gctttgaaga acttgggctt caacttttac aacagcttta agaggtgttg gatgtttgcc atggggatca acaccgtaaa gtgcaatacc ggggcgaaca agatcaaaat aaaaatctga accaagaaaa atgcctccag aattggcaaa tgagacttta caggtaggta actgtgcaag tactctcttg aaagcagcta attgtttatt attggatgaa tgagaggcgt cctctccatt cgcaaggtga ctgagaatat attttatttc tgctttttca aaaattgtag ggtttttgat gagtttttgt aattcttttt tatcaagccc taatcggctc atattggtat cgacttgaat gattgcggga aattttttat tctttttttg gcaaagtgtt tgccaatctt cgatagtaga ccaagagttc aaaagaggga taatacccga ttgtgcgaca aattcttccg ctttatgtgg aaaaccattc agaagagcaa tcatgacatt ttctggtaaa acggctttta attgaagcgc ttcttcgatt tgggcaacaa aaaaagtgcg gcaaccagct tgataaagtg ccggggcaat tttatgtgcc cctaacccat aggcattagc tttcactacc gctgaacact cagttggagc aacatgttgg gctaaagttc tataatttgc aacaatagca cgcacgtcga tggttgctat agctgtagca ggaaataatg ggtttgctgt atccttagcg gatttgttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNKSAKDTA NPLFPATAIA TIDVRAIVAN YRTLAQHVAP TECSAVVKAN AYGLGAHKIA PALYQAGCRT FFVAQIEEAL QLKAVLPENV MIALLNGFPH KAEEFVAQSG IIPLLNSWST IEDWQTLCQK KNKKFPAIIQ VDTNMSRLGL DKKELQKLIK NPTIFEKAEI KYILSHLANG EDASHSSNNK QLAAFKRVLA QLPTCKVSFA NSGGIFLGSD FYFDLVRPGI ALYGVDPHGK HPTPLKAVVK VEAQVLQSRF IDAGIPVGYR ESFMTRRPST LATISIGYAD GWPRILSNKG TVYFNGHKLP IVGHISMDSI IVDATDLDKK PQRGDWVELI GPHQPLEKVS TDTNTIPHEI LTSLGKRYKR IYI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACAAATCC GCTAAGGATA CAGCAAACCC ATTATTTCCT GCTACAGCTA TAGCAACCAT CGACGTGCGT GCTATTGTTG CAAATTATAG AACTTTAGCC CAACATGTTG CTCCAACTGA GTGTTCAGCG GTAGTGAAAG CTAATGCCTA TGGGTTAGGG GCACATAAAA TTGCCCCGGC ACTTTATCAA GCTGGTTGCC GCACTTTTTT TGTTGCCCAA ATCGAAGAAG CGCTTCAATT AAAAGCCGTT TTACCAGAAA ATGTCATGAT TGCTCTTCTG AATGGTTTTC CACATAAAGC GGAAGAATTT GTCGCACAAT CGGGTATTAT CCCTCTTTTG AACTCTTGGT CTACTATCGA AGATTGGCAA ACACTTTGCC AAAAAAAGAA TAAAAAATTT CCCGCAATCA TTCAAGTCGA TACCAATATG AGCCGATTAG GGCTTGATAA AAAAGAATTA CAAAAACTCA TCAAAAACCC TACAATTTTT GAAAAAGCAG AAATAAAATA TATTCTCAGT CACCTTGCGA ATGGAGAGGA CGCCTCTCAT TCATCCAATA ATAAACAATT AGCTGCTTTC AAGAGAGTAC TTGCACAGTT ACCTACCTGT AAAGTCTCAT TTGCCAATTC TGGAGGCATT TTTCTTGGTT CAGATTTTTA TTTTGATCTT GTTCGCCCCG GTATTGCACT TTACGGTGTT GATCCCCATG GCAAACATCC AACACCTCTT AAAGCTGTTG TAAAAGTTGA AGCCCAAGTT CTTCAAAGCC GCTTTATTGA CGCAGGAATA CCTGTTGGTT ATCGAGAAAG CTTTATGACC CGCAGACCAA GCACTCTTGC AACCATTTCT ATTGGCTATG CAGATGGTTG GCCACGGATT CTTTCGAATA AAGGTACCGT TTATTTCAAT GGGCACAAAC TCCCTATTGT TGGACATATT TCTATGGATT CTATCATAGT CGATGCTACT GATCTCGATA AAAAACCTCA AAGAGGTGAT TGGGTAGAGC TCATTGGCCC TCATCAACCC CTTGAAAAAG TATCTACAGA TACCAATACC ATTCCTCATG AAATTCTCAC TTCTCTAGGA AAACGCTATA AGCGCATTTA CATTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BaheA.00339.a.A1.PW27468
| PURIFICATION DATe: | 7/14/2009 |
| CONCENTRATION: | 19.62mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNKSAKDTA NPLFPATAIA TIDVRAIVAN YRTLAQHVAP TECSAVVKAN AYGLGAHKIA PALYQAGCRT FFVAQIEEAL QLKAVLPENV MIALLNGFPH KAEEFVAQSG IIPLLNSWST IEDWQTLCQK KNKKFPAIIQ VDTNMSRLGL DKKELQKLIK NPTIFEKAEI KYILSHLANG EDASHSSNNK QLAAFKRVLA QLPTCKVSFA NSGGIFLGSD FYFDLVRPGI ALYGVDPHGK HPTPLKAVVK VEAQVLQSRF IDAGIPVGYR ESFMTRRPST LATISIGYAD GWPRILSNKG TVYFNGHKLP IVGHISMDSI IVDATDLDKK PQRGDWVELI GPHQPLEKVS TDTNTIPHEI LTSLGKRYKR I
Validated NT Sequence
aaatgcgctt atagcgtttt cctagagaag tgagaatttc atgaggaatg gtattggtat ctgtagatac tttttcaagg ggttgatgag ggccaatgag ctctacccaa tcacctcttt gaggtttttt atcgagatca gtagcatcga ctatgataga atccatagaa atatgtccaa caatagggag tttgtgccca ttgaaataaa cggtaccttt attcgaaaga atccgtggcc aaccatctgc atagccaata gaaatggttg caagagtgct tggtctgcgg gtcataaagc tttctcgata accaacaggt attcctgcgt caataaagcg gctttgaaga acttgggctt caacttttac aacagcttta agaggtgttg gatgtttgcc atggggatca acaccgtaaa gtgcaatacc ggggcgaaca agatcaaaat aaaaatctga accaagaaaa atgcctccag aattggcaaa tgagacttta caggtaggta actgtgcaag tactctcttg aaagcagcta attgtttatt attggatgaa tgagaggcgt cctctccatt cgcaaggtga ctgagaatat attttatttc tgctttttca aaaattgtag ggtttttgat gagtttttgt aattcttttt tatcaagccc taatcggctc atattggtat cgacttgaat gattgcggga aattttttat tctttttttg gcaaagtgtt tgccaatctt cgatagtaga ccaagagttc aaaagaggga taatacccga ttgtgcgaca aattcttccg ctttatgtgg aaaaccattc agaagagcaa tcatgacatt ttctggtaaa acggctttta attgaagcgc ttcttcgatt tgggcaacaa aaaaagtgcg gcaaccagct tgataaagtg ccggggcaat tttatgtgcc cctaacccat aggcattagc tttcactacc gctgaacact cagttggagc aacatgttgg gctaaagttc tataatttgc aacaatagca cgcacgtcga tggttgctat agctgtagca ggaaataatg ggtttgctgt atccttagcg gatttgttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNKSAKDTA NPLFPATAIA TIDVRAIVAN YRTLAQHVAP TECSAVVKAN AYGLGAHKIA PALYQAGCRT FFVAQIEEAL QLKAVLPENV MIALLNGFPH KAEEFVAQSG IIPLLNSWST IEDWQTLCQK KNKKFPAIIQ VDTNMSRLGL DKKELQKLIK NPTIFEKAEI KYILSHLANG EDASHSSNNK QLAAFKRVLA QLPTCKVSFA NSGGIFLGSD FYFDLVRPGI ALYGVDPHGK HPTPLKAVVK VEAQVLQSRF IDAGIPVGYR ESFMTRRPST LATISIGYAD GWPRILSNKG TVYFNGHKLP IVGHISMDSI IVDATDLDKK PQRGDWVELI GPHQPLEKVS TDTNTIPHEI LTSLGKRYKR IYI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACAAATCC GCTAAGGATA CAGCAAACCC ATTATTTCCT GCTACAGCTA TAGCAACCAT CGACGTGCGT GCTATTGTTG CAAATTATAG AACTTTAGCC CAACATGTTG CTCCAACTGA GTGTTCAGCG GTAGTGAAAG CTAATGCCTA TGGGTTAGGG GCACATAAAA TTGCCCCGGC ACTTTATCAA GCTGGTTGCC GCACTTTTTT TGTTGCCCAA ATCGAAGAAG CGCTTCAATT AAAAGCCGTT TTACCAGAAA ATGTCATGAT TGCTCTTCTG AATGGTTTTC CACATAAAGC GGAAGAATTT GTCGCACAAT CGGGTATTAT CCCTCTTTTG AACTCTTGGT CTACTATCGA AGATTGGCAA ACACTTTGCC AAAAAAAGAA TAAAAAATTT CCCGCAATCA TTCAAGTCGA TACCAATATG AGCCGATTAG GGCTTGATAA AAAAGAATTA CAAAAACTCA TCAAAAACCC TACAATTTTT GAAAAAGCAG AAATAAAATA TATTCTCAGT CACCTTGCGA ATGGAGAGGA CGCCTCTCAT TCATCCAATA ATAAACAATT AGCTGCTTTC AAGAGAGTAC TTGCACAGTT ACCTACCTGT AAAGTCTCAT TTGCCAATTC TGGAGGCATT TTTCTTGGTT CAGATTTTTA TTTTGATCTT GTTCGCCCCG GTATTGCACT TTACGGTGTT GATCCCCATG GCAAACATCC AACACCTCTT AAAGCTGTTG TAAAAGTTGA AGCCCAAGTT CTTCAAAGCC GCTTTATTGA CGCAGGAATA CCTGTTGGTT ATCGAGAAAG CTTTATGACC CGCAGACCAA GCACTCTTGC AACCATTTCT ATTGGCTATG CAGATGGTTG GCCACGGATT CTTTCGAATA AAGGTACCGT TTATTTCAAT GGGCACAAAC TCCCTATTGT TGGACATATT TCTATGGATT CTATCATAGT CGATGCTACT GATCTCGATA AAAAACCTCA AAGAGGTGAT TGGGTAGAGC TCATTGGCCC TCATCAACCC CTTGAAAAAG TATCTACAGA TACCAATACC ATTCCTCATG AAATTCTCAC TTCTCTAGGA AAACGCTATA AGCGCATTTA CATTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA