BaheA.00313.a

ATP-dependent RNA helicase

CENTER ID: BaheA.00313.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00313.a.A1.GE25712 Full length( BaheA.00313.a ) 1 467
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00313.a.A1.PW25760 Full length( BaheA.00313.a ) 1 467

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.704
RefSeq: YP_033476.1
UniProt: A0A0H3LY24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPPLNNFDD LGLSAKVIKA VKSVGYTVPT PIQSGTIPHV LQRRDVLGIA QTGTGKTASF VLPMLTLLEK GRARARMPRT LILEPTRELA AQVEENFDKY GINHRLNVAL LIGGVSFEQQ DRKLERGVDV LIATPGRLLD HFERGKLLLM GVEILVIDEA DRMLDMGFIP EIERICKLTP FTRQTLFFSA TMAPEITKLT KQFLHSPVTV EVTKASSTAS TITQRLVKSG NKAWDKRAVL RELIHNEGDK LKNAIIFCNR KRDISELLRS LIRHNFSVGA LHGDMDQYSR MSTLADFKDN KITLLVASDV AARGLDIPAV SHVFNYDVPT HAEDYIHRIG RTGRANRSGK AFTIVTKTDQ KYINAIEEIS NEKIEWLTGD LSTLTTTDQK DDIILKRKSP TSSKKTAQKE RSVHTQKSLK DDKVGYMKAT NNKVNLLQKC HSRKNKNHTP SGFGDDIPAF MLIKIRT
NT Sequence
ATGACACCAC CTTTAAATAA TTTTGATGAT TTAGGTCTTT CTGCAAAGGT TATCAAGGCA GTAAAGTCAG TTGGATACAC TGTTCCAACA CCTATTCAGA GTGGTACAAT TCCACATGTA CTACAGAGAA GAGACGTTTT AGGAATAGCC CAAACAGGAA CTGGAAAAAC AGCCTCTTTC GTTTTGCCTA TGCTAACTCT CCTTGAAAAA GGCCGTGCAA GAGCGCGTAT GCCCCGTACT CTCATACTAG AACCAACACG GGAACTTGCA GCCCAAGTTG AAGAAAATTT CGATAAATAC GGAATAAATC ATCGTTTAAA TGTTGCTCTG CTCATCGGTG GCGTGTCTTT TGAACAGCAA GATCGTAAAC TTGAAAGGGG AGTTGATGTT CTTATAGCAA CACCAGGACG TCTTCTTGAC CATTTCGAAC GCGGTAAATT GCTCTTAATG GGTGTTGAAA TTCTTGTTAT TGATGAAGCT GATCGTATGT TGGATATGGG TTTTATTCCT GAGATTGAAC GCATCTGTAA GCTAACCCCC TTTACGCGTC AAACTTTGTT CTTCTCCGCA ACAATGGCGC CAGAAATCAC AAAACTAACA AAGCAATTTT TACATTCTCC TGTGACTGTT GAAGTTACAA AAGCATCCTC GACAGCTAGC ACAATTACAC AACGGCTCGT CAAATCTGGA AATAAGGCAT GGGATAAAAG AGCTGTTTTG CGAGAACTTA TCCACAATGA AGGTGATAAA CTTAAAAATG CTATTATTTT TTGTAACCGA AAGAGAGATA TCTCTGAACT TTTGAGATCG CTCATTAGAC ATAATTTTAG TGTAGGGGCA CTTCATGGCG ATATGGACCA ATATTCTCGT ATGAGTACAT TGGCTGACTT TAAAGACAAC AAAATCACAC TTCTTGTCGC CTCTGATGTG GCTGCACGAG GACTTGATAT TCCAGCCGTA AGCCATGTCT TCAATTATGA TGTTCCTACA CACGCTGAAG ACTACATTCA TAGAATTGGT CGTACAGGAC GTGCAAATCG TAGCGGAAAA GCTTTTACGA TTGTCACAAA AACTGATCAA AAATATATAA ATGCCATTGA AGAAATAAGC AACGAAAAAA TTGAATGGCT TACTGGAGAT CTCTCTACTT TAACAACCAC TGATCAAAAA GATGATATCA TTTTAAAGAG AAAATCTCCA ACATCGTCAA AGAAAACTGC TCAAAAAGAG CGTTCTGTTC ATACCCAAAA GTCCTTGAAA GATGATAAAG TTGGTTATAT GAAAGCCACG AATAATAAAG TCAATCTCCT TCAAAAATGT CATTCTCGAA AAAATAAAAA TCATACTCCC TCTGGATTTG GTGATGATAT ACCGGCATTT ATGCTCATAA AAATCCGCAC T
Details for BaheA.00313.a.A1.GE25712
HARVESTED ON: 10/17/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPPLNNFD DLGLSAKVIK AVKSVGYTVP TPIQSGTIPH VLQRRDVLGI AQTGTGKTAS FVLPMLTLLE KGRARARMPR TLILEPTREL AAQVEENFDK YGINHRLNVA LLIGGVSFEQ QDRKLERGVD VLIATPGRLL DHFERGKLLL MGVEILVIDE ADRMLDMGFI PEIERICKLT PFTRQTLFFS ATMAPEITKL TKQFLHSPVT VEVTKASSTA STITQRLVKS GNKAWDKRAV LRELIHNEGX NLKMLLFFVX XREXSLNFXX RSLDIILX
Validated NT Sequence
tttaagaagg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacacc acctttaaat aattttgatg atttaggtct ttctgcaaag gttatcaagg cagtaaagtc agttggatac actgttccaa cacctattca gagtggtaca attccacatg tactacagag aagagacgtt ttaggaatag cccaaacagg aactggaaaa acagcctctt tcgttttgcc tatgctaact ctccttgaaa aaggccgtgc aagagcgcgt atgccccgta ctctcatact agaaccaaca cgggaacttg cagcccaagt tgaagaaaat ttcgataaat acggaataaa tcatcgttta aatgttgctc tgctcatcgg tggcgtgtct tttgaacagc aagatcgtaa acttgaaagg ggagttgatg ttcttatagc aacaccagga cgtcttcttg accatttcga acgcggtaaa ttgctcttaa tgggtgttga aattcttgtt attgatgaag ctgatcgtat gttggatatg ggttttattc ctgagattga acgcatctgt aagctaaccc cctttacgcg tcaaactttg ttcttctccg caacaatggc gccagaaatc acaaaactaa caaagcaatt tttacattct cctgtgactg ttgaagttac aaaagcatcc tcgacagcta gcacaattac acaacggctc gtcaaatctg gaaataaggc atgggataaa agagctgttt tgcgagaact tatccacaat gaaggtgana acttaaaaat gctattattt tttgtancng naagagagan atctctgaac ttttnagntc gctcattaga cataatttta gngtaggggc anttcatggc gatanggacc antattctcg tatgagtnca ttggctgact ttaaagncaa caaaatcaca cttcttgtcg cntntgatgt ggctgcacga ggacttgata ttccagccgt aagccatgtc ttcaattatg atgttcctac acacgctgaa gactacattc atagaattgg tcgtacagga cgtgcaaatc gtagcggaaa agcttttacg attgtcacaa aaactgatca aaaatatata aatgccattg aagaaataag caacgaaaaa attgaatggc ttactggaga tctctctact ttaacaacca ctgatcaaaa agatgatatc attttaaaga gaaaatctcc aacatcgtca aagaaaactg ctcaaaaaga gcgttctgtt catacccaaa agtccttgaa agatgataaa gttggttata tgaaagccac gaataataaa gtcaatctcc ttcaaaaatg tcattctcga aaaaataaaa atcatactcc ctctggattt ggtgatgata taccggcatt tatgctcata aaaatccgca cttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPPLNNFD DLGLSAKVIK AVKSVGYTVP TPIQSGTIPH VLQRRDVLGI AQTGTGKTAS FVLPMLTLLE KGRARARMPR TLILEPTREL AAQVEENFDK YGINHRLNVA LLIGGVSFEQ QDRKLERGVD VLIATPGRLL DHFERGKLLL MGVEILVIDE ADRMLDMGFI PEIERICKLT PFTRQTLFFS ATMAPEITKL TKQFLHSPVT VEVTKASSTA STITQRLVKS GNKAWDKRAV LRELIHNEGD KLKNAIIFCN RKRDISELLR SLIRHNFSVG ALHGDMDQYS RMSTLADFKD NKITLLVASD VAARGLDIPA VSHVFNYDVP THAEDYIHRI GRTGRANRSG KAFTIVTKTD QKYINAIEEI SNEKIEWLTG DLSTLTTTDQ KDDIILKRKS PTSSKKTAQK ERSVHTQKSL KDDKVGYMKA TNNKVNLLQK CHSRKNKNHT PSGFGDDIPA FMLIKIRT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaccacct ttaaataatt ttgatgattt aggtctttct gcaaaggtta tcaaggcagt aaagtcagtt ggatacactg ttccaacacc tattcagagt ggtacaattc cacatgtact acagagaaga gacgttttag gaatagccca aacaggaact ggaaaaacag cctctttcgt tttgcctatg ctaactctcc ttgaaaaagg ccgtgcaaga gcgcgtatgc cccgtactct catactagaa ccaacacggg aacttgcagc ccaagttgaa gaaaatttcg ataaatacgg aataaatcat cgtttaaatg ttgctctgct catcggtggc gtgtcttttg aacagcaaga tcgtaaactt gaaaggggag ttgatgttct tatagcaaca ccaggacgtc ttcttgacca tttcgaacgc ggtaaattgc tcttaatggg tgttgaaatt cttgttattg atgaagctga tcgtatgttg gatatgggtt ttattcctga gattgaacgc atctgtaagc taaccccctt tacgcgtcaa actttgttct tctccgcaac aatggcgcca gaaatcacaa aactaacaaa gcaattttta cattctcctg tgactgttga agttacaaaa gcatcctcga cagctagcac aattacacaa cggctcgtca aatctggaaa taaggcatgg gataaaagag ctgttttgcg agaacttatc cacaatgaag gtgataaact taaaaatgct attatttttt gtaaccgaaa gagagatatc tctgaacttt tgagatcgct cattagacat aattttagtg taggggcact tcatggcgat atggaccaat attctcgtat gagtacattg gctgacttta aagacaacaa aatcacactt cttgtcgcct ctgatgtggc tgcacgagga cttgatattc cagccgtaag ccatgtcttc aattatgatg ttcctacaca cgctgaagac tacattcata gaattggtcg tacaggacgt gcaaatcgta gcggaaaagc ttttacgatt gtcacaaaaa ctgatcaaaa atatataaat gccattgaag aaataagcaa cgaaaaaatt gaatggctta ctggagatct ctctacttta acaaccactg atcaaaaaga tgatatcatt ttaaagagaa aatctccaac atcgtcaaag aaaactgctc aaaaagagcg ttctgttcat acccaaaagt ccttgaaaga tgataaagtt ggttatatga aagccacgaa taataaagtc aatctccttc aaaaatgtca ttctcgaaaa aataaaaatc atactccctc tggatttggt gatgatatac cggcatttat gctcataaaa atccgcacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BaheA.00313.a.A1.PW25760
PURIFICATION DATe: 2/24/2009
CONCENTRATION: 16.63mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 61
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPPLNNFD DLGLSAKVIK AVKSVGYTVP TPIQSGTIPH VLQRRDVLGI AQTGTGKTAS FVLPMLTLLE KGRARARMPR TLILEPTREL AAQVEENFDK YGINHRLNVA LLIGGVSFEQ QDRKLERGVD VLIATPGRLL DHFERGKLLL MGVEILVIDE ADRMLDMGFI PEIERICKLT PFTRQTLFFS ATMAPEITKL TKQFLHSPVT VEVTKASSTA STITQRLVKS GNKAWDKRAV LRELIHNEGX NLKMLLFFVX XREXSLNFXX RSLDIILX
Validated NT Sequence
tttaagaagg agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacacc acctttaaat aattttgatg atttaggtct ttctgcaaag gttatcaagg cagtaaagtc agttggatac actgttccaa cacctattca gagtggtaca attccacatg tactacagag aagagacgtt ttaggaatag cccaaacagg aactggaaaa acagcctctt tcgttttgcc tatgctaact ctccttgaaa aaggccgtgc aagagcgcgt atgccccgta ctctcatact agaaccaaca cgggaacttg cagcccaagt tgaagaaaat ttcgataaat acggaataaa tcatcgttta aatgttgctc tgctcatcgg tggcgtgtct tttgaacagc aagatcgtaa acttgaaagg ggagttgatg ttcttatagc aacaccagga cgtcttcttg accatttcga acgcggtaaa ttgctcttaa tgggtgttga aattcttgtt attgatgaag ctgatcgtat gttggatatg ggttttattc ctgagattga acgcatctgt aagctaaccc cctttacgcg tcaaactttg ttcttctccg caacaatggc gccagaaatc acaaaactaa caaagcaatt tttacattct cctgtgactg ttgaagttac aaaagcatcc tcgacagcta gcacaattac acaacggctc gtcaaatctg gaaataaggc atgggataaa agagctgttt tgcgagaact tatccacaat gaaggtgana acttaaaaat gctattattt tttgtancng naagagagan atctctgaac ttttnagntc gctcattaga cataatttta gngtaggggc anttcatggc gatanggacc antattctcg tatgagtnca ttggctgact ttaaagncaa caaaatcaca cttcttgtcg cntntgatgt ggctgcacga ggacttgata ttccagccgt aagccatgtc ttcaattatg atgttcctac acacgctgaa gactacattc atagaattgg tcgtacagga cgtgcaaatc gtagcggaaa agcttttacg attgtcacaa aaactgatca aaaatatata aatgccattg aagaaataag caacgaaaaa attgaatggc ttactggaga tctctctact ttaacaacca ctgatcaaaa agatgatatc attttaaaga gaaaatctcc aacatcgtca aagaaaactg ctcaaaaaga gcgttctgtt catacccaaa agtccttgaa agatgataaa gttggttata tgaaagccac gaataataaa gtcaatctcc ttcaaaaatg tcattctcga aaaaataaaa atcatactcc ctctggattt ggtgatgata taccggcatt tatgctcata aaaatccgca cttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPPLNNFD DLGLSAKVIK AVKSVGYTVP TPIQSGTIPH VLQRRDVLGI AQTGTGKTAS FVLPMLTLLE KGRARARMPR TLILEPTREL AAQVEENFDK YGINHRLNVA LLIGGVSFEQ QDRKLERGVD VLIATPGRLL DHFERGKLLL MGVEILVIDE ADRMLDMGFI PEIERICKLT PFTRQTLFFS ATMAPEITKL TKQFLHSPVT VEVTKASSTA STITQRLVKS GNKAWDKRAV LRELIHNEGD KLKNAIIFCN RKRDISELLR SLIRHNFSVG ALHGDMDQYS RMSTLADFKD NKITLLVASD VAARGLDIPA VSHVFNYDVP THAEDYIHRI GRTGRANRSG KAFTIVTKTD QKYINAIEEI SNEKIEWLTG DLSTLTTTDQ KDDIILKRKS PTSSKKTAQK ERSVHTQKSL KDDKVGYMKA TNNKVNLLQK CHSRKNKNHT PSGFGDDIPA FMLIKIRT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACCACCT TTAAATAATT TTGATGATTT AGGTCTTTCT GCAAAGGTTA TCAAGGCAGT AAAGTCAGTT GGATACACTG TTCCAACACC TATTCAGAGT GGTACAATTC CACATGTACT ACAGAGAAGA GACGTTTTAG GAATAGCCCA AACAGGAACT GGAAAAACAG CCTCTTTCGT TTTGCCTATG CTAACTCTCC TTGAAAAAGG CCGTGCAAGA GCGCGTATGC CCCGTACTCT CATACTAGAA CCAACACGGG AACTTGCAGC CCAAGTTGAA GAAAATTTCG ATAAATACGG AATAAATCAT CGTTTAAATG TTGCTCTGCT CATCGGTGGC GTGTCTTTTG AACAGCAAGA TCGTAAACTT GAAAGGGGAG TTGATGTTCT TATAGCAACA CCAGGACGTC TTCTTGACCA TTTCGAACGC GGTAAATTGC TCTTAATGGG TGTTGAAATT CTTGTTATTG ATGAAGCTGA TCGTATGTTG GATATGGGTT TTATTCCTGA GATTGAACGC ATCTGTAAGC TAACCCCCTT TACGCGTCAA ACTTTGTTCT TCTCCGCAAC AATGGCGCCA GAAATCACAA AACTAACAAA GCAATTTTTA CATTCTCCTG TGACTGTTGA AGTTACAAAA GCATCCTCGA CAGCTAGCAC AATTACACAA CGGCTCGTCA AATCTGGAAA TAAGGCATGG GATAAAAGAG CTGTTTTGCG AGAACTTATC CACAATGAAG GTGATAAACT TAAAAATGCT ATTATTTTTT GTAACCGAAA GAGAGATATC TCTGAACTTT TGAGATCGCT CATTAGACAT AATTTTAGTG TAGGGGCACT TCATGGCGAT ATGGACCAAT ATTCTCGTAT GAGTACATTG GCTGACTTTA AAGACAACAA AATCACACTT CTTGTCGCCT CTGATGTGGC TGCACGAGGA CTTGATATTC CAGCCGTAAG CCATGTCTTC AATTATGATG TTCCTACACA CGCTGAAGAC TACATTCATA GAATTGGTCG TACAGGACGT GCAAATCGTA GCGGAAAAGC TTTTACGATT GTCACAAAAA CTGATCAAAA ATATATAAAT GCCATTGAAG AAATAAGCAA CGAAAAAATT GAATGGCTTA CTGGAGATCT CTCTACTTTA ACAACCACTG ATCAAAAAGA TGATATCATT TTAAAGAGAA AATCTCCAAC ATCGTCAAAG AAAACTGCTC AAAAAGAGCG TTCTGTTCAT ACCCAAAAGT CCTTGAAAGA TGATAAAGTT GGTTATATGA AAGCCACGAA TAATAAAGTC AATCTCCTTC AAAAATGTCA TTCTCGAAAA AATAAAAATC ATACTCCCTC TGGATTTGGT GATGATATAC CGGCATTTAT GCTCATAAAA ATCCGCACTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A