BaheA.00293.a

DNA polymerase, bacteriophage type

CENTER ID: BaheA.00293.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00293.a.B1.GE27118 Full length( BaheA.00293.a ) 1 275
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.526
RefSeq: YP_033321.1
UniProt: A0A0H3LXU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNHTPCSIT YEELLSFYKE SGVDTALTDL PIDRFSQSAS LEKKSTQVVK ISHNQQTSPS IKLDNHPLSN STIIQGELSA IESAKNANTL DELKSALLAF NGCSLKLTAK NTCFSDGTAG SPLMLIGEAP GREEDIQGIP FVGKAGMLLN KILASIGLTR NSVYIANTIP WRPPGNRTPT PREVALCRPF IERQIYLANP RILIALGGVA TQFLTGAQNG IIRTRGKWLT YKSENNIKIP VMPTFHPAYL LRTPSQKKLT WLDFLEVKNR LDSLL
NT Sequence
ATGTTAAATC ATACTCCGTG CTCAATCACA TATGAAGAAC TGTTAAGCTT CTATAAAGAA AGCGGTGTAG ATACTGCCTT AACAGATCTA CCTATTGATC GCTTTAGTCA ATCTGCTTCT TTAGAAAAAA AATCAACACA AGTAGTAAAG ATTTCTCATA ATCAACAGAC ATCGCCATCC ATAAAGTTAG ACAATCATCC GCTTTCTAAC TCTACTATCA TACAAGGTGA ACTTTCGGCT ATAGAGAGTG CAAAAAATGC GAATACACTT GATGAATTAA AATCTGCTCT CCTCGCGTTT AATGGCTGCT CATTAAAATT GACAGCAAAA AATACCTGTT TTTCAGATGG AACAGCAGGA AGTCCTCTCA TGCTTATAGG AGAAGCACCA GGACGAGAGG AAGATATACA AGGAATTCCT TTTGTAGGGA AAGCAGGAAT GTTACTGAAT AAAATTCTTG CATCAATTGG CTTAACAAGA AATAGTGTTT ACATAGCTAA TACTATTCCT TGGCGTCCAC CAGGAAATCG CACACCCACG CCAAGAGAAG TTGCATTATG CCGCCCTTTC ATTGAACGGC AAATTTATTT AGCTAATCCT CGTATTCTTA TAGCACTGGG AGGGGTTGCG ACACAGTTCC TTACGGGAGC GCAAAATGGA ATTATCCGAA CCCGAGGTAA ATGGCTCACC TATAAAAGCG AGAACAACAT AAAAATACCT GTTATGCCAA CCTTTCACCC TGCTTATCTT CTTCGAACGC CGAGTCAGAA AAAGCTTACG TGGCTAGACT TTCTAGAAGT AAAAAATCGC TTAGACAGCC TTTTA
Details for BaheA.00293.a.B1.GE27118
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 40
Validated AA Sequence
MLXNKILASI GLTRNSVYIA NTIPWRXPGN XTPTPREVAL XRPFIXRQVX LXNPXILIAL GGVATQFLTG AQNGIIRTRG KWLTYKSENN IKIPVMPTFH PAYLLRTPSQ KKLRG
Validated NT Sequence
tttacttcta gaaagtctag ccacgaagct ttttctgact cggcgttcga agaagataag cagggtgaaa ggttggcata acaggtattt ttatgttgtt ctcgctttta taggtgagcc atttacctcg ggttcggata attccatttt gcgctcccgt aaggaactgt gtcgcaaccc ctcccagngc tataagaata cnaggattan ctaantaaac ttgccgntca atgaaagggc ggnataatgc aacttctctt ggcgtgggtg tgcnatttcc tggtgnacgc caaggaatag tattagctat gtaaacacta tttcttgtta agccaattga tgcaagaatt ttattcanta acattcctgc tttccctaca naaggaattc cttgtatatc ttccnctcgn cctgnnnnnn ntcctataan nnnganagga cttcctgctg ttccntctga aaaacaggta ttttnnnctn tcaannntna tgagcagcca tnnnncncga ggagagcann nnnnaattca tcaagnntnn nngcattttt tncncnctcn ntannnnnna gttcacctng tntnatngnn gannnnnnaa nnngatgatn gtcnancnnn ntnnnnggcg atgtntnnng nttatgagaa atctttacta cttgtgttga ttttttttnt aaagaagcag attgantaaa gcgntcnntn ggtagatctg ttaaggcagt atctacaccg ctttcttnat agaagcntaa cagttcttca tatgngatng agc
Expected Protein Sequence
MAHHHHHHML NHTPCSITYE ELLSFYKESG VDTALTDLPI DRFSQSASLE KKSTQVVKIS HNQQTSPSIK LDNHPLSNST IIQGELSAIE SAKNANTLDE LKSALLAFNG CSLKLTAKNT CFSDGTAGSP LMLIGEAPGR EEDIQGIPFV GKAGMLLNKI LASIGLTRNS VYIANTIPWR PPGNRTPTPR EVALCRPFIE RQIYLANPRI LIALGGVATQ FLTGAQNGII RTRGKWLTYK SENNIKIPVM PTFHPAYLLR TPSQKKLTWL DFLEVKNRLD SLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa atcatactcc gtgctcaatc acatatgaag aactgttaag cttctataaa gaaagcggtg tagatactgc cttaacagat ctacctattg atcgctttag tcaatctgct tctttagaaa aaaaatcaac acaagtagta aagatttctc ataatcaaca gacatcgcca tccataaagt tagacaatca tccgctttct aactctacta tcatacaagg tgaactttcg gctatagaga gtgcaaaaaa tgcgaataca cttgatgaat taaaatctgc tctcctcgcg tttaatggct gctcattaaa attgacagca aaaaatacct gtttttcaga tggaacagca ggaagtcctc tcatgcttat aggagaagca ccaggacgag aggaagatat acaaggaatt ccttttgtag ggaaagcagg aatgttactg aataaaattc ttgcatcaat tggcttaaca agaaatagtg tttacatagc taatactatt ccttggcgtc caccaggaaa tcgcacaccc acgccaagag aagttgcatt atgccgccct ttcattgaac ggcaaattta tttagctaat cctcgtattc ttatagcact gggaggggtt gcgacacagt tccttacggg agcgcaaaat ggaattatcc gaacccgagg taaatggctc acctataaaa gcgagaacaa cataaaaata cctgttatgc caacctttca ccctgcttat cttcttcgaa cgccgagtca gaaaaagctt acgtggctag actttctaga agtaaaaaat cgcttagaca gccttttatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 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