SSGCID: Target Page

BaheA.00241.a

tryptophanyl-tRNA synthetase

CENTER ID:	BaheA.00241.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00241.a.A1.GE25701	Full length( BaheA.00241.a )		1	356

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00241.a.A1.PW25749	Full length( BaheA.00241.a )		1	356

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1477
RefSeq:	YP_034071.1
UniProt:	A0A0H3LXR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTFTPLVFS GVQPSGHLHL GNYLGAIKHW VELQTSYNCL YCVVDMHALT VNPDPLALRE STRAVTAAFL AAGIDPKKHI IFNQSQVFQH AELAWILNCI ARIGWLQRMT QFKDKAGKDR EKASLGLFAY PSLMAADILL YRATHVPVGE DQKQHVELTR DIAQKFNNDY AQRIVDLNFS VTMQMGEEKK QVFFPLPKAL IGDTAMRVMS LRDGTKKMSK SDPSDFSRIN LTDDADLIAK KIRKAKTDSA PLPDTLAALE ERPEIDNLLG IYAAFAQISK EKALSEFSGQ QFSLFKTTLA DLAVEKLAPI TQELRRLHQE SSYIDSVLHD GAQRASALAE KNMKKVREIV GFLHNI

NT Sequence

ATGGACACCT TCACACCACT TGTCTTTTCC GGAGTACAAC CGAGTGGTCA CTTACATCTT GGCAATTATC TTGGAGCCAT CAAGCATTGG GTTGAACTGC AAACGTCCTA TAATTGTCTG TACTGCGTTG TTGATATGCA TGCCCTCACC GTTAATCCGG ATCCACTTGC CTTGCGTGAA TCTACCAGAG CAGTTACAGC AGCCTTTTTA GCTGCTGGTA TTGATCCAAA AAAACACATT ATTTTTAATC AATCTCAAGT TTTTCAACAT GCCGAACTCG CATGGATTCT AAATTGTATC GCCCGTATTG GTTGGCTCCA GCGCATGACA CAATTTAAAG ATAAAGCAGG AAAAGATCGT GAAAAAGCAT CCCTTGGACT TTTTGCTTAT CCAAGTCTGA TGGCTGCCGA TATTTTACTC TATCGTGCCA CACATGTTCC AGTGGGCGAA GATCAAAAAC AGCATGTTGA ACTCACCCGT GATATTGCTC AAAAATTCAA TAATGATTAT GCACAGCGTA TTGTAGATTT AAACTTTTCT GTTACAATGC AAATGGGTGA GGAAAAAAAA CAAGTTTTTT TCCCTCTTCC AAAGGCGCTG ATAGGGGATA CAGCTATGCG CGTTATGTCG TTGCGCGATG GCACAAAAAA AATGTCGAAA TCGGATCCTT CCGATTTTTC GCGCATTAAT CTAACAGATG ATGCTGATCT TATCGCAAAA AAAATACGCA AAGCAAAAAC GGACTCCGCC CCTCTTCCCG ACACGCTTGC AGCCTTAGAA GAACGGCCAG AAATCGATAA CCTGCTTGGT ATTTATGCTG CCTTTGCACA AATAAGCAAA GAAAAAGCTC TTTCAGAGTT TTCCGGACAA CAATTTTCGC TTTTTAAAAC AACCTTAGCT GACCTTGCTG TCGAGAAGCT TGCACCAATC ACCCAAGAAC TCCGTCGACT TCATCAAGAA AGTAGTTATA TTGACTCTGT TTTGCACGAT GGTGCACAAC GTGCCAGTGC ATTAGCAGAA AAAAATATGA AAAAAGTCCG TGAAATTGTT GGATTTTTGC ACAATATA

Details for BaheA.00241.a.A1.GE25701

HARVESTED ON:	10/17/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDTFTPLVF SGVQPSGHLH LGNYLGAIKH WVELQTSYNC LYCVVDMHAL TVNPDPLALR ESTRAVTAAF LAAGIDPKKH IIFNQSQVFQ HAELAWILNC IARIGWLQRM TQFKDKAGKD REKASLGLFA YPSLMAADIL LYRATHVPVG EDQKQHVELT RDIAQKFNND YAQRIVDLNF SVTMQMGEEK KQVFFPLPKA LIGETAMRVM SLRDGTKKMS KSDPSDFSRI NLTDDADLIA KKIRKAKTDS APLPDTLAAL EERPEIDNLL GIYAAFAQIS KEKALSEFSG QQFSLFKTTL ADLAVEKLAP ITQELRRLHQ ESSYIDSVLH DGAQRASALA EKNMKKVREI VGF

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca ccttcacacc acttgtcttt tccggagtac aaccgagtgg tcacttacat cttggcaatt atcttggagc catcaagcat tgggttgaac tgcaaacgtc ctataattgt ctgtactgcg ttgttgatat gcatgccctc accgttaatc cggatccact tgccttgcgt gaatctacca gagcagttac agcagccttt ttagctgctg gtattgatcc aaaaaaacac attattttta atcaatctca agtttttcaa catgccgaac tcgcatggat tctaaattgt atcgcccgta ttggttggct ccagcgcatg acacaattta aagataaagc aggaaaagat cgtgaaaaag catcccttgg actttttgct tatccaagtc tgatggctgc cgatatttta ctctatcgtg ccacacatgt tccagtgggc gaagatcaaa aacagcatgt tgaactcacc cgtgatattg ctcaaaaatt caataatgat tatgcacagc gtattgtaga tttaaacttt tctgttacaa tgcaaatggg tgaggaaaaa aaacaagttt ttttccctct tccaaaggcg ctgatagggg aaacagctat gcgcgttatg tcgttgcgcg atggcacaaa aaaaatgtcg aaatcggatc cttccgattt ttcgcgcatt aatctaacag atgatgctga tcttatcgca aaaaaaatac gcaaagcaaa aacggactcc gcccctcttc ccgacacgct tgcagcctta gaagaacggc cagaaatcga taacctgctt ggtatttatg ctgcctttgc acaaataagc aaagaaaaag ctctttcaga gttttccgga caacaatttt cgctttttaa aacaacctta gctgaccttg ctgtcgagaa gcttgcacca atcacccaag aactccgtcg acttcatcaa gaaagtagtt atattgactc tgttttgcac gatggtgcac aacgtgccag tgcattagca gaaaaaaata tgaaaaaagt ccgtgaaatt gttggatttt tg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTFTPLVF SGVQPSGHLH LGNYLGAIKH WVELQTSYNC LYCVVDMHAL TVNPDPLALR ESTRAVTAAF LAAGIDPKKH IIFNQSQVFQ HAELAWILNC IARIGWLQRM TQFKDKAGKD REKASLGLFA YPSLMAADIL LYRATHVPVG EDQKQHVELT RDIAQKFNND YAQRIVDLNF SVTMQMGEEK KQVFFPLPKA LIGDTAMRVM SLRDGTKKMS KSDPSDFSRI NLTDDADLIA KKIRKAKTDS APLPDTLAAL EERPEIDNLL GIYAAFAQIS KEKALSEFSG QQFSLFKTTL ADLAVEKLAP ITQELRRLHQ ESSYIDSVLH DGAQRASALA EKNMKKVREI VGFLHNI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaccttc acaccacttg tcttttccgg agtacaaccg agtggtcact tacatcttgg caattatctt ggagccatca agcattgggt tgaactgcaa acgtcctata attgtctgta ctgcgttgtt gatatgcatg ccctcaccgt taatccggat ccacttgcct tgcgtgaatc taccagagca gttacagcag cctttttagc tgctggtatt gatccaaaaa aacacattat ttttaatcaa tctcaagttt ttcaacatgc cgaactcgca tggattctaa attgtatcgc ccgtattggt tggctccagc gcatgacaca atttaaagat aaagcaggaa aagatcgtga aaaagcatcc cttggacttt ttgcttatcc aagtctgatg gctgccgata ttttactcta tcgtgccaca catgttccag tgggcgaaga tcaaaaacag catgttgaac tcacccgtga tattgctcaa aaattcaata atgattatgc acagcgtatt gtagatttaa acttttctgt tacaatgcaa atgggtgagg aaaaaaaaca agtttttttc cctcttccaa aggcgctgat aggggataca gctatgcgcg ttatgtcgtt gcgcgatggc acaaaaaaaa tgtcgaaatc ggatccttcc gatttttcgc gcattaatct aacagatgat gctgatctta tcgcaaaaaa aatacgcaaa gcaaaaacgg actccgcccc tcttcccgac acgcttgcag ccttagaaga acggccagaa atcgataacc tgcttggtat ttatgctgcc tttgcacaaa taagcaaaga aaaagctctt tcagagtttt ccggacaaca attttcgctt tttaaaacaa ccttagctga ccttgctgtc gagaagcttg caccaatcac ccaagaactc cgtcgacttc atcaagaaag tagttatatt gactctgttt tgcacgatgg tgcacaacgt gccagtgcat tagcagaaaa aaatatgaaa aaagtccgtg aaattgttgg atttttgcac aatataaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BaheA.00241.a.A1.PW25749

PURIFICATION DATe:	9/8/2009
CONCENTRATION:	15mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTFTPLVF SGVQPSGHLH LGNYLGAIKH WVELQTSYNC LYCVVDMHAL TVNPDPLALR ESTRAVTAAF LAAGIDPKKH IIFNQSQVFQ HAELAWILNC IARIGWLQRM TQFKDKAGKD REKASLGLFA YPSLMAADIL LYRATHVPVG EDQKQHVELT RDIAQKFNND YAQRIVDLNF SVTMQMGEEK KQVFFPLPKA LIGDTAMRVM SLRDGTKKMS KSDPSDFSRI NLTDDADLIA KKIRKAKTDS APLPDTLAAL EERPEIDNLL GIYAAFAQIS KEKALSEFSG QQFSLFKTTL ADLAVEKLAP ITQELRRLHQ ESSYIDSVLH DGAQRASALA EKNMKKVREI VGFLHNI

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACACCTTC ACACCACTTG TCTTTTCCGG AGTACAACCG AGTGGTCACT TACATCTTGG CAATTATCTT GGAGCCATCA AGCATTGGGT TGAACTGCAA ACGTCCTATA ATTGTCTGTA CTGCGTTGTT GATATGCATG CCCTCACCGT TAATCCGGAT CCACTTGCCT TGCGTGAATC TACCAGAGCA GTTACAGCAG CCTTTTTAGC TGCTGGTATT GATCCAAAAA AACACATTAT TTTTAATCAA TCTCAAGTTT TTCAACATGC CGAACTCGCA TGGATTCTAA ATTGTATCGC CCGTATTGGT TGGCTCCAGC GCATGACACA ATTTAAAGAT AAAGCAGGAA AAGATCGTGA AAAAGCATCC CTTGGACTTT TTGCTTATCC AAGTCTGATG GCTGCCGATA TTTTACTCTA TCGTGCCACA CATGTTCCAG TGGGCGAAGA TCAAAAACAG CATGTTGAAC TCACCCGTGA TATTGCTCAA AAATTCAATA ATGATTATGC ACAGCGTATT GTAGATTTAA ACTTTTCTGT TACAATGCAA ATGGGTGAGG AAAAAAAACA AGTTTTTTTC CCTCTTCCAA AGGCGCTGAT AGGGGATACA GCTATGCGCG TTATGTCGTT GCGCGATGGC ACAAAAAAAA TGTCGAAATC GGATCCTTCC GATTTTTCGC GCATTAATCT AACAGATGAT GCTGATCTTA TCGCAAAAAA AATACGCAAA GCAAAAACGG ACTCCGCCCC TCTTCCCGAC ACGCTTGCAG CCTTAGAAGA ACGGCCAGAA ATCGATAACC TGCTTGGTAT TTATGCTGCC TTTGCACAAA TAAGCAAAGA AAAAGCTCTT TCAGAGTTTT CCGGACAACA ATTTTCGCTT TTTAAAACAA CCTTAGCTGA CCTTGCTGTC GAGAAGCTTG CACCAATCAC CCAAGAACTC CGTCGACTTC ATCAAGAAAG TAGTTATATT GACTCTGTTT TGCACGATGG TGCACAACGT GCCAGTGCAT TAGCAGAAAA AAATATGAAA AAAGTCCGTG AAATTGTTGG ATTTTTGCAC AATATAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA