BaheA.00239.a
glutathione reductase
| CENTER ID: | BaheA.00239.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00239.a.A1.GE25700 | Full length( BaheA.00239.a ) | 1 | 463 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00239.a.A1.PW25748 | Full length( BaheA.00239.a ) | 1 | 463 |
Structures
3O0H
DEPOSITED: 7/19/2010
DETERMINATION: XRay
CLONE: BaheA.00239.a.A1.GE25700
PROTEIN: BaheA.00239.a.A1.PW25748
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126785 |
| PATRIC ID: | fig|283166.5.peg.700 |
| RefSeq: | YP_033472.1 |
| UniProt: | A0A0H3LWY9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSFDFDLFV IGSGSGGVRA ARLAGALGKR VAIAEEYRIG GTCVIRGCVP KKLYFYASQY AQEFSKSIGF GWKYADPIFN WEKLVAAKNK EISRLEGLYR EGLQNSNVHI YESRAVFVDE HTLELSVTGE RISAEKILIA TGAKIVSNSA IKGSDLCLTS NEIFDLEKLP KSIVIVGGGY IGVEFANIFH GLGVKTTLLH RGDLILRNFD YDLRQLLNDA MVAKGISIIY EATVSQVQST ENCYNVVLTN GQTICADRVM LATGRVPNTT GLGLERAGVK VNEFGAVVVD EKMTTNVSHI WAVGDVTGHI QLTPVAIHDA MCFVKNAFEN TSTTPDYDLI TTAVFSQPEI GTVGLSEEDA LHRYKRVEIY RTVFRPMRNV LSGSPEKMFM KLVVDGESRI VVGAHVLGEN AGEIAQLIGI SLKGKLTKDI FDKTMAVHPT MSEELVTMYK PSYVYENGEK LDN
NT Sequence
GTGGGATCTT TTGACTTTGA TTTGTTTGTT ATTGGAAGTG GTTCTGGTGG AGTGCGTGCT GCACGCCTTG CTGGAGCACT CGGCAAGCGT GTTGCAATAG CAGAAGAGTA TCGTATAGGA GGAACTTGCG TTATTCGTGG TTGCGTCCCC AAAAAACTCT ATTTTTATGC ATCACAATAT GCACAAGAGT TTAGTAAAAG TATTGGTTTT GGATGGAAAT ACGCTGATCC AATTTTTAAC TGGGAAAAAT TGGTTGCGGC TAAAAATAAA GAAATATCAA GATTAGAAGG ACTTTATCGC GAGGGGCTAC AAAATAGCAA TGTTCATATT TATGAAAGTC GTGCTGTTTT TGTGGATGAG CATACATTAG AACTCTCTGT GACAGGTGAA CGGATAAGCG CAGAAAAAAT TTTAATTGCC ACGGGGGCAA AAATTGTATC AAACTCTGCC ATAAAAGGGA GTGATTTATG TCTTACTTCC AATGAAATTT TTGATCTTGA AAAACTACCA AAATCAATAG TCATTGTTGG GGGAGGGTAT ATTGGCGTTG AATTTGCTAA CATTTTTCAT GGATTAGGTG TAAAAACAAC GCTCCTCCAT CGTGGTGATT TAATTCTTCG TAATTTTGAT TATGATTTGC GTCAGTTGCT TAATGATGCA ATGGTTGCAA AAGGGATTTC TATTATCTAT GAAGCAACTG TTTCTCAAGT TCAGTCTACA GAGAATTGTT ATAATGTTGT TTTAACAAAT GGACAAACGA TTTGTGCCGA TCGAGTTATG TTGGCTACGG GGCGTGTGCC GAATACAACG GGTTTAGGAC TTGAGCGCGC AGGCGTTAAA GTGAATGAAT TTGGTGCAGT TGTTGTTGAT GAAAAAATGA CGACAAATGT ATCTCATATT TGGGCTGTTG GTGATGTAAC AGGTCATATT CAATTGACAC CTGTTGCTAT TCATGATGCA ATGTGTTTCG TTAAGAATGC ATTTGAGAAT ACATCCACGA CACCTGATTA TGATCTAATC ACAACAGCGG TTTTTTCACA GCCCGAAATT GGAACGGTTG GTCTTTCAGA AGAAGATGCA CTCCATCGTT ATAAGCGTGT CGAGATTTAT CGTACAGTTT TTCGTCCTAT GCGCAATGTT CTTTCCGGGA GTCCGGAAAA AATGTTTATG AAGCTTGTTG TTGATGGTGA AAGCCGTATT GTTGTGGGAG CCCATGTTTT AGGGGAAAAT GCTGGTGAGA TAGCACAGCT TATTGGCATA TCTCTCAAGG GAAAACTAAC GAAGGATATT TTCGATAAAA CTATGGCAGT GCATCCAACG ATGTCAGAAG AATTGGTAAC GATGTATAAA CCAAGTTATG TGTATGAAAA TGGGGAAAAA CTGGACAAT
Details for BaheA.00239.a.A1.GE25700
| HARVESTED ON: | 10/17/2008 |
| SEQUENCED ON: | 3/27/2012 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 66 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSFDFDL FVIGSGSGGV RAARLAGALG KRVAIAEEYR IGGTCVIRGC VPKKLYFYAS QYAQEFSKSI GFGWKYADPI FNWEKLVAAK NKEISRLEGL YREGLQNSNV HIYESRAVFV DEHTLELSVT GERISAEKIL IATGAKIVSN SAIKGSDLCL TSNEIFDLEK LPKSIVIVGG GYIGVEFANI FHGLGVKTTL LHRGDLILRN FDYDLRQLLN DAMVAKGISI IYEATVSQVQ STENCYNVVL TNGQTICADR VMLATGRVPN TTGLGLERAG VKVNEFGAXV VDEKMTTXVS HIW
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gatcttttga ctttgatttg tttgttattg gaagtggttc tggtggagtg cgtgctgcac gccttgctgg agcactcggc aagcgtgttg caatagcaga agagtatcgt ataggaggaa cttgcgttat tcgtggttgc gtccccaaaa aactctattt ttatgcatca caatatgcac aagagtttag taaaagtatt ggttttggat ggaaatacgc tgatccaatt tttaactggg aaaaattggt tgcggctaaa aataaagaaa tatcaagatt agaaggactt tatcgcgagg ggctacaaaa tagcaatgtt catatttatg aaagtcgtgc tgtttttgtg gatgagcata cattagaact ctctgtgaca ggtgaacgga taagcgcaga aaaaatttta attgccacgg gggcaaaaat tgtatcaaac tctgccataa aagggagtga tttatgtctt acttccaatg aaatttttga tcttgaaaaa ctaccaaaat caatagtcat tgttggggga gggtatattg gcgttgaatt tgctaacatt tttcatggat taggtgtaaa aacaacgctt ctccatcgtg gtgatttaat tcttcgtaat tttgattatg atttgcgtca gttgcttaat gatgcaatgg ttgcaaaagg gatttctatt atctatgaag caactgtttc tcaagttcag tctacagaga attgttataa tgttgtttta acaaatggac aaacgatttg tgccgatcga gttatgttgg ctacggggcg tgtgccgaat acnacgggtt taggacttga gcgcgcaggc gttaaagtga atgaatttgg tgcantngtt gttgatgaaa aaatgacgac nanngtatcn catatttggg ct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSFDFDL FVIGSGSGGV RAARLAGALG KRVAIAEEYR IGGTCVIRGC VPKKLYFYAS QYAQEFSKSI GFGWKYADPI FNWEKLVAAK NKEISRLEGL YREGLQNSNV HIYESRAVFV DEHTLELSVT GERISAEKIL IATGAKIVSN SAIKGSDLCL TSNEIFDLEK LPKSIVIVGG GYIGVEFANI FHGLGVKTTL LHRGDLILRN FDYDLRQLLN DAMVAKGISI IYEATVSQVQ STENCYNVVL TNGQTICADR VMLATGRVPN TTGLGLERAG VKVNEFGAVV VDEKMTTNVS HIWAVGDVTG HIQLTPVAIH DAMCFVKNAF ENTSTTPDYD LITTAVFSQP EIGTVGLSEE DALHRYKRVE IYRTVFRPMR NVLSGSPEKM FMKLVVDGES RIVVGAHVLG ENAGEIAQLI GISLKGKLTK DIFDKTMAVH PTMSEELVTM YKPSYVYENG EKLDN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggatct tttgactttg atttgtttgt tattggaagt ggttctggtg gagtgcgtgc tgcacgcctt gctggagcac tcggcaagcg tgttgcaata gcagaagagt atcgtatagg aggaacttgc gttattcgtg gttgcgtccc caaaaaactc tatttttatg catcacaata tgcacaagag tttagtaaaa gtattggttt tggatggaaa tacgctgatc caatttttaa ctgggaaaaa ttggttgcgg ctaaaaataa agaaatatca agattagaag gactttatcg cgaggggcta caaaatagca atgttcatat ttatgaaagt cgtgctgttt ttgtggatga gcatacatta gaactctctg tgacaggtga acggataagc gcagaaaaaa ttttaattgc cacgggggca aaaattgtat caaactctgc cataaaaggg agtgatttat gtcttacttc caatgaaatt tttgatcttg aaaaactacc aaaatcaata gtcattgttg ggggagggta tattggcgtt gaatttgcta acatttttca tggattaggt gtaaaaacaa cgctcctcca tcgtggtgat ttaattcttc gtaattttga ttatgatttg cgtcagttgc ttaatgatgc aatggttgca aaagggattt ctattatcta tgaagcaact gtttctcaag ttcagtctac agagaattgt tataatgttg ttttaacaaa tggacaaacg atttgtgccg atcgagttat gttggctacg gggcgtgtgc cgaatacaac gggtttagga cttgagcgcg caggcgttaa agtgaatgaa tttggtgcag ttgttgttga tgaaaaaatg acgacaaatg tatctcatat ttgggctgtt ggtgatgtaa caggtcatat tcaattgaca cctgttgcta ttcatgatgc aatgtgtttc gttaagaatg catttgagaa tacatccacg acacctgatt atgatctaat cacaacagcg gttttttcac agcccgaaat tggaacggtt ggtctttcag aagaagatgc actccatcgt tataagcgtg tcgagattta tcgtacagtt tttcgtccta tgcgcaatgt tctttccggg agtccggaaa aaatgtttat gaagcttgtt gttgatggtg aaagccgtat tgttgtggga gcccatgttt taggggaaaa tgctggtgag atagcacagc ttattggcat atctctcaag ggaaaactaa cgaaggatat tttcgataaa actatggcag tgcatccaac gatgtcagaa gaattggtaa cgatgtataa accaagttat gtgtatgaaa atggggaaaa actggacaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BaheA.00239.a.A1.PW25748
| PURIFICATION DATe: | 2/22/2010 |
| CONCENTRATION: | 29.62mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 66 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSFDFDL FVIGSGSGGV RAARLAGALG KRVAIAEEYR IGGTCVIRGC VPKKLYFYAS QYAQEFSKSI GFGWKYADPI FNWEKLVAAK NKEISRLEGL YREGLQNSNV HIYESRAVFV DEHTLELSVT GERISAEKIL IATGAKIVSN SAIKGSDLCL TSNEIFDLEK LPKSIVIVGG GYIGVEFANI FHGLGVKTTL LHRGDLILRN FDYDLRQLLN DAMVAKGISI IYEATVSQVQ STENCYNVVL TNGQTICADR VMLATGRVPN TTGLGLERAG VKVNEFGAXV VDEKMTTXVS HIW
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gatcttttga ctttgatttg tttgttattg gaagtggttc tggtggagtg cgtgctgcac gccttgctgg agcactcggc aagcgtgttg caatagcaga agagtatcgt ataggaggaa cttgcgttat tcgtggttgc gtccccaaaa aactctattt ttatgcatca caatatgcac aagagtttag taaaagtatt ggttttggat ggaaatacgc tgatccaatt tttaactggg aaaaattggt tgcggctaaa aataaagaaa tatcaagatt agaaggactt tatcgcgagg ggctacaaaa tagcaatgtt catatttatg aaagtcgtgc tgtttttgtg gatgagcata cattagaact ctctgtgaca ggtgaacgga taagcgcaga aaaaatttta attgccacgg gggcaaaaat tgtatcaaac tctgccataa aagggagtga tttatgtctt acttccaatg aaatttttga tcttgaaaaa ctaccaaaat caatagtcat tgttggggga gggtatattg gcgttgaatt tgctaacatt tttcatggat taggtgtaaa aacaacgctt ctccatcgtg gtgatttaat tcttcgtaat tttgattatg atttgcgtca gttgcttaat gatgcaatgg ttgcaaaagg gatttctatt atctatgaag caactgtttc tcaagttcag tctacagaga attgttataa tgttgtttta acaaatggac aaacgatttg tgccgatcga gttatgttgg ctacggggcg tgtgccgaat acnacgggtt taggacttga gcgcgcaggc gttaaagtga atgaatttgg tgcantngtt gttgatgaaa aaatgacgac nanngtatcn catatttggg ct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSFDFDL FVIGSGSGGV RAARLAGALG KRVAIAEEYR IGGTCVIRGC VPKKLYFYAS QYAQEFSKSI GFGWKYADPI FNWEKLVAAK NKEISRLEGL YREGLQNSNV HIYESRAVFV DEHTLELSVT GERISAEKIL IATGAKIVSN SAIKGSDLCL TSNEIFDLEK LPKSIVIVGG GYIGVEFANI FHGLGVKTTL LHRGDLILRN FDYDLRQLLN DAMVAKGISI IYEATVSQVQ STENCYNVVL TNGQTICADR VMLATGRVPN TTGLGLERAG VKVNEFGAVV VDEKMTTNVS HIWAVGDVTG HIQLTPVAIH DAMCFVKNAF ENTSTTPDYD LITTAVFSQP EIGTVGLSEE DALHRYKRVE IYRTVFRPMR NVLSGSPEKM FMKLVVDGES RIVVGAHVLG ENAGEIAQLI GISLKGKLTK DIFDKTMAVH PTMSEELVTM YKPSYVYENG EKLDN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGATCT TTTGACTTTG ATTTGTTTGT TATTGGAAGT GGTTCTGGTG GAGTGCGTGC TGCACGCCTT GCTGGAGCAC TCGGCAAGCG TGTTGCAATA GCAGAAGAGT ATCGTATAGG AGGAACTTGC GTTATTCGTG GTTGCGTCCC CAAAAAACTC TATTTTTATG CATCACAATA TGCACAAGAG TTTAGTAAAA GTATTGGTTT TGGATGGAAA TACGCTGATC CAATTTTTAA CTGGGAAAAA TTGGTTGCGG CTAAAAATAA AGAAATATCA AGATTAGAAG GACTTTATCG CGAGGGGCTA CAAAATAGCA ATGTTCATAT TTATGAAAGT CGTGCTGTTT TTGTGGATGA GCATACATTA GAACTCTCTG TGACAGGTGA ACGGATAAGC GCAGAAAAAA TTTTAATTGC CACGGGGGCA AAAATTGTAT CAAACTCTGC CATAAAAGGG AGTGATTTAT GTCTTACTTC CAATGAAATT TTTGATCTTG AAAAACTACC AAAATCAATA GTCATTGTTG GGGGAGGGTA TATTGGCGTT GAATTTGCTA ACATTTTTCA TGGATTAGGT GTAAAAACAA CGCTCCTCCA TCGTGGTGAT TTAATTCTTC GTAATTTTGA TTATGATTTG CGTCAGTTGC TTAATGATGC AATGGTTGCA AAAGGGATTT CTATTATCTA TGAAGCAACT GTTTCTCAAG TTCAGTCTAC AGAGAATTGT TATAATGTTG TTTTAACAAA TGGACAAACG ATTTGTGCCG ATCGAGTTAT GTTGGCTACG GGGCGTGTGC CGAATACAAC GGGTTTAGGA CTTGAGCGCG CAGGCGTTAA AGTGAATGAA TTTGGTGCAG TTGTTGTTGA TGAAAAAATG ACGACAAATG TATCTCATAT TTGGGCTGTT GGTGATGTAA CAGGTCATAT TCAATTGACA CCTGTTGCTA TTCATGATGC AATGTGTTTC GTTAAGAATG CATTTGAGAA TACATCCACG ACACCTGATT ATGATCTAAT CACAACAGCG GTTTTTTCAC AGCCCGAAAT TGGAACGGTT GGTCTTTCAG AAGAAGATGC ACTCCATCGT TATAAGCGTG TCGAGATTTA TCGTACAGTT TTTCGTCCTA TGCGCAATGT TCTTTCCGGG AGTCCGGAAA AAATGTTTAT GAAGCTTGTT GTTGATGGTG AAAGCCGTAT TGTTGTGGGA GCCCATGTTT TAGGGGAAAA TGCTGGTGAG ATAGCACAGC TTATTGGCAT ATCTCTCAAG GGAAAACTAA CGAAGGATAT TTTCGATAAA ACTATGGCAG TGCATCCAAC GATGTCAGAA GAATTGGTAA CGATGTATAA ACCAAGTTAT GTGTATGAAA ATGGGGAAAA ACTGGACAAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA